ABSTRACT
Objective: To identificate the bioactive natural products, and study the chemical constituents in the flower buds of Rosa rugosa. Methods: The chemical constituents in the flower buds of R. rugosa were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatography, and high performance liquid chromatography (HPLC) methods. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Results: Nine flavonoids were isolated from the flower buds of R. rugosa, and identified as 8-acetyl-4',7-dimethoxy-6-methyl-flavone (1), kaempferol (2), kaempferol-3-O-β-D-glucopyranoside (3), kaempferol-3-O-β-D-rutinside (4), quercetin (5), quercetin-3-O-β-D-glucopyranoside (6), rutin (7), luteolin (8), and luteolin-7-O-β-D-glucopyranoside (9). Conclusion: Compound 1 is a new compound and it displays the cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC50 values of 6.8, 5.6, 2.2, 1.8, and 7.4 μmol/L, respectively. Compound 9 is isolated from R. rugosa for the first time.
ABSTRACT
Objective: To study the chemical constituents from the herbs of Bidens bipinnata. Methods: The compounds were isolated and purified by various column chromatographies. Their structures were elucidated by means of physicochemical properties and spectral analyses (MS, 1H-NMR, and 13C-NMR). Results: Eighteen compounds were isolated from 95% ethanol extract of B. bipinnata and identified as quercetin-5-O-β-D-glucopyranoside (1), okanin-4′-O-β-D-(2″, 4″, 6″-triacetyl)-glucopyranoside (2), okanin-4′-O-β-D-(3″, 4″-diacetyl-6″-trans-p-coumaroyl)-glucopyranoside (3), luteolin-7-O-β-D-glucopyranoside (4), kaempferol-7-O-β-D-glucopyranoside (5), kaempferol-3-O-β-D-rutinoside (6), quercetin-3-O-β-D-glucopyranoside (7), quercetin-7-O-β-D-glucopyranoside (8), hyperoside (9), rutin (10), naringenin (11), apigenin (12), quercetin (13), 5, 7-dihydroxy chromone-7-O-β-D-glucoside (14), caffeic acid (15), gallic acid (16), β-sitosterol (17), and sitosterol-3-O-glucopyranoside (18). Conclusion: Compounds 1 and 11 are obtained from the plants in Bidens L. for the first time, and compounds 3-7, 14, 15, and 18 are firstly obtained from this plant.