ABSTRACT
Objective: To study the chemical constituents from the roots of Pfaffia paniculata. Methods: The compounds were isolated and purified by means of chromatographic separation techniques and their structures were identified based on spectral features. Results: Fourteen known compounds, including 11 30-noroleanane triterpenoids and three ecdysterones, named pfaffianol A (1), 3-oxo-akebonoic acid (2), 16β-hydroxyl-3-oxo-akebonoic acid (3), pfaffiaglycoside A (4), pfameric acid (5), 2α,3β,20α-trihydroxy-30-norolean-12-en-28-oic acid (6), 2α,3β-dihydroxy-23-oxo-30-norolean-12,20(29)-dien-28-oic acid (7), 2α,3β-dihydroxy-30-norolean-12,20(29)-dien-28-oic acid (8), 2α,3α-dihydroxy-30-norolean-12,20(29)-dien-28-oic acid (9), quinatic acid (10), 3β-hydroxy-30-norhederagenin (11), diaulusterol B (12), 20,22-didehydrotaxisterone (13), and ecdysterone (14) were isolated from the roots of P. paniculata. Conclusion: Compounds 10-12 are obtained from P. paniculata for the first time. Compounds 6-9, and 13 are isolated from the genus of Pfaffia for the first time.
ABSTRACT
Objective: To establish and optimize the technique of tissue culture and rapid propagation of Pfaffia paniculata. Methods: Using MS and 1/2MS basic media, the effects of various combinations of plant growth regulators (6-BA, NAA, IBA, and IAA) on the seedlings subculture and rooting culture were studied by orthogonal design. Results: The effective medium for cluster buds-inducing and subculture was MS + 6-BA 1.5 mg/L + NAA 0.1 mg/L + IBA 0.2 mg/L, and the propagation coefficient was over 6.0 per 30 d; The best rooting medium was 1/2 MS + NAA 1.0 mg/L + IBA 0.2 mg/L, and the rooting rate was 100%. The rooting seedlings were transplanted in well-drained sand bed and the survival rate was 98%. Conclusion: The method in the experiment could provide a basis on protecting the mild resources of P. paniculata exploring the artificial resources, and discussing the new way breedings.