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OBJECTIVE: To analyze the differences in chemical composition of Pseudostellariae Radix from different germplasms. METHODS: Tweny-four batches of samples were determined by UPLC-Triple TOF-MS/MS. Characteristic peaks were extracted through treatment of mass spectrometry data including peak matching, peak alignment and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. Component identification was carried out based on the mass spectrometry accurate mass and secondary mass spectrometry fragmentation information combined with database search and literature. RESULTS: The differences among samples of Pseudostellariae Radix of different germplasms were distinguishable. Twenty-one chemical compositions with significant differences were screened, among which 10 common chemical compositions showed different changing laws. CONCLUSION: This study provides experimental data for revealing the laws of Pseudostellariae Radix metabolites from different germplasms.
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[ ABSTRACT] AIM:To study the role of Radix Pseudostellariae polysaccharide ( RPP) in hepatic insulin resist-ance.METHODS:Six-week-old C57BL/6J mice were randomly divided into low-fat diet (LFD) control group and high-fat diet ( HFD) model group.After 16 weeks, intraperitoneal pyruvate tolerance test ( IPPTT) was performed to determine the establishment of the HFD-induced hepatic insulin resistance model.HFD containing RPP (500 mg/kg) was given for 4 consecutive weeks.IPPTT, liver malondialdehyde ( MDA) level and liver mitochondrial MDA level were measured.The protein levels of p-AKT (Ser473/Thr308), p-AMPK, nuclear factor E2-related factor 2 (Nrf2), NQO1 and IκBαin the liver tissues were measured by Western blot.RESULTS:After administration of RPP, a significant reduction in the levels of blood glucose and hepatic mitochondrial MDA was observed.The levels of p-AKT ( Ser473/Thr308) and p-AMPK were significantly elevated in the liver tissues.The hepatic IκBαlevels were up-regulated.RPP also enhanced the expression of Nrf2 system-regulated proteins NQO1 and HO-1 in the liver tissues.CONCLUSION:Radix Pseudostellariae polysaccha-rides effectively reduce HFD-induced hepatic insulin resistance in C57BL/6J mice and improves liver glucose metabolism by ameliorating HFD-impaired hepatic transduction of insulin signaling, activating Nrf2-associated signaling and inhibiting the expression of inflammatory signaling proteins.
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Objective:To investigate the ameliorated effects of n-butanol extract from Radix Pseudostellariae(RP) on rat experimental cardiopulmonary Injury induced by acute myocardial infarction.Methods:The experimental rat cardiac and lung injury was reproduced by acute cardiac infraction ligating left coronary artery for 4 weeks.Cardiac function were assayed by hemodynamics,heart index and lung index were detected by weighting,pathologic change was checked out by histophathology,and cardiac infarction size was assayed by image analysis system.Results:After operation 4 weeks,cardiac function was disturbed according to the results of hemodynamics.Cardiac and lung index,and cardiac infarction size was significantly increased in model group.Results of histopathology indicated that cardiac myocyte decrease,cardiac fibrosis functional disorder of the heart,increase of heart index and lun index,inflammatory cell infiltration,and pulmonary congestion and edema and so on indicated the model was succeed.Continued administration of n-butanol extract from RP for 4 weeks can remarkable ameliorate cardiac function,decrease the cardiac and lung index,and attenuate the heart failure pathological change.Conclusion:N-butanol extract from RP can alleviate rat cardiopulmonary injury induced by acute myocardial infarction.The present research provided the pharmacological base of replenishing qi for invigorating the spleen and promoting body fluid production for nourishing lung.
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AIM: To investigate the effects of radix pseudostellariae on cardiac function and matrix metalloproteinases(MMPs) in rat chronic heart failure induced by coronary artery ligation.METHODS: Rat model of chronic heart failure was produced by ligating the coronary artery for 6 weeks.The cardiac functions were determined by observing the hemodynamics parameters.mRNA expressions of MMP-2 and MMP-9 in left ventricular tissue were detected by RT-PCR and activities of MMPs were assayed by zemography.RESULTS: Cardiac function was remarkable turbulence after coronary artery ligation 6 weeks,according to the results of the hemodynamics.The mRNA contents and activities of MMP-2 and MMP-9 in left ventricular tissue were significantly elevated.Radix pseudostellariae remarkable ameliorated the cardiac functions,inhibited the mRNA contents and activities of MMP-2 and MMP-9 in left ventricular tissue of rat with chronic heart failure after continued 5 weeks treated with decoction of radix pseudostellariae.CONCLUSION: The decoction of radix pseudostellariae ameliorates cardiac functions of rat with chronic heart failure.The mechanism maybe involves in the inhibition of the MMP-2 and MMP-9 mRNA expression and activities in cardiac ventricles.
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AIM:To establish the analytical method for the fingerprint of Radix Pseudostellariae by HPLC-MS and to estimate the quality of Radix Pseudostellariae from different habitats. METHODS: Radix Pseudostellariae from different habitats were analyzed and the total ion current(TIC) chromatographic fingerprint were determined by HPLC-MS.Some characteristic peaks were identified preliminarily based on the MS spectra and literature data. RESULTS: HPLC-MS fingerprint of 10 main peaks was established preliminarily.It was found that a small number of samples differed from others obviously. CONCLUSION: The method is reliable,accurate and can be used for quality control of Radix Pseudostellariae.