ABSTRACT
Study of secondary plant products is important for understanding their functions and for obtaining biologically active compounds for practical use in different spheres of human activity. Among other plant substances, phytoecdysteroids attract great scientific interest, because of their potential contribution to crop protection strategies and their possible medical uses. Silene repens is widespread in Mongolia. Seed of Silene repens were collected in 2008 from Mongolian and Russian Altai and were cultivated in Siberian Botanical Garden at Tomsk State University, Russia.GoalStudy the dynamics of the 20-hydroxyecdysone in various plant organs of cultivated Silene repens.Material and MethodsThe seasonal dynamics of phytoecdysteroid content (20-hydroxyecdysone) observed in the vegetative and generative organs of cultivated Silene repens was investigated. The quantity of phytoecdysteroids was determined by spectrophotometer UV-1800 (Shimadzu, Japan).ResultsThe content of 20-hydroxyecdysone varied from 0.001 to 1.07 % depending on the organ and growth stage. The maximum content of 20-hydroxyecdysone was observed in actively developing parts of organs: during beginning vegetation in roots (0.3 %); during budding, in the bud (1.00 %); during flowering, in the flowers (1.07 %); during fruiting, in roots (0.31 %). The general trends seen in the distribution of 20-hydroxyecdysone found for cultivated Silene repens from Mongolian and Russian Altai during the plant growth cycle. A high content of 20-hydroxyecdysone during flowering and a low amount during fruiting.ConclusionA high content of 20-hydroxyecdysone during flowering in the flowers and buds (1.07 -1,00 %) and low content of 20-hydroxyecdysone during fruiting in the stem (0.001-0.12 %). The seasonal dynamics of phytoecdysteroids in cultivated Silene repens has been studied for the first time.
ABSTRACT
Silene is a genus of the Caryophyllaceae family, contains more than 700 species, which are widely distributed in Northern Hemisphere, but also in Africa, Asia and South American. Phytochemical investigations of Silene species have revealed that many components from this genus are highly bioactive. More than 400 compounds has been isolated, among them major are phytoecdysteroids. The paper reviews the biological (antitumour, antibacterial and antioxidant) activities and the phytoecdysteroids of genus Silene. We summarized the phytoecdysteroids content referring to 171 species from the genus Silene and list 93 phytoecdysteroids isolated over the past few decades. There are also reports on the mentioned folk and traditional effects of Silene plants.
Silene es un género de la familia Caryophyllaceae, conteniendo más de 70 especies, ampliamente distribuidas no solo en el hemisferio norte, sino que también en Africa, Asia y sud América. Investigaciones fotoquímicas de las especies de Silene han revelado que muchos componentes de este género son altamente bioactivos. Más de 400 compuestos han sido aislados, entre ellos los mayoritarios correspondientes a fitoecdiesteroides. El manuscrito revisa las actividades biológicas (antitumorales, antibacterianas y antioxidantes) y los fitoecdiesteroides del género Silene. Resumimos el contenido de fitoecdiesteroides en 171 especies del género Silene y listamos 93 fitoecdiesteroides aislados desde hace unas pocas décadas. Hay también reportes mencionados acerca de los efectos tradicionales y folclóricos de la plantas del género Silene.
Subject(s)
Anti-Bacterial Agents , Antineoplastic Agents, Phytogenic , Antioxidants , Ecdysteroids , Plants, Medicinal/classification , Silene , Ethnopharmacology , Medicine, Traditional , Species SpecificityABSTRACT
BackgroundPhytoecdysteroids are secondary metabolites produced by many plants. Ecdysteroid containing plants have widely been used in medicine and show pharmacological effects such as: anabolic, adaptogenic, genoprotection, hepatoprotection, antiradiation, anti-inflammatory, antidiabetic and anticancerous.GoalThe aim of this study was to determination, isolation and purification of phytoecdysteroids in the Mongolian flora.Materials and МetodsPlants collected during flowering in western and central regions of Mongolia in 2006-2008 and its flowering stage. Plants speciesclassification was identified by Professor D. Oyunchimeg (Department of botany, Hovd university, Mongolia), Professor A. I Pyak and A. L. Ebeli (Department of botany, Tomsk State University, Russia). The main ecdysteroid components were identified in Silene repens by HPLC using the UV spectrometry. The absorption maximum is due to the presence of a conjugated ketone and occurs near 240-250 nm.Results The ecdysteroid containing plants of Mongolian flora has been studied for the first time. Total 297 species from 171 genera in 50 families for ecdysteroid were screened. Most of them represent a random sampling of plants growing in the selected regions. The highest concentration of ecdysteroids was detected in the family of Caryophyllaceae (genera Silene, Elisanthe, Melandrium), Chenopodiaceae (genera Chenopodium and Axyris) and Asteraceae (genera Rhaponticum and Serratula). Ecdysteroids were investigated in 16 from 297 species of the families Сaryophyllaсеае, Asteraceae and Chenopodiaceae of Mongolian flora, but in 6 of them: Silene ichebogdo, Silene mongolica, Elisanthe aprica, Serratula marginata, Chenopodium frutescens, Axyris prostrate at the first time. Ecdysteroid profile has isolated from aerial parts of Silene repens 5 phytoecdysteroids such as: 2-deoxyecdysone, 2-deoxy-20-hydroxyecdysone, polypodine B, 20-hydroxyecdysone, integristerone A and identified by column chromatography and HPLC methods.ConclusionEcdysteroid profile of 297 plant species was investigated. Five ecdysteroids were isolated, their structure were analyzed by HPLC analysis and UV spectrometry in Silene repens. The basic esdysteroids concentrations in plants of Silene jenissensis, Silene repens, Serratula marginata, Stemmacantha uniflorum and Axyris prostrate L. comprise 2.2%, 1.1%, 1.0%, 1.3% and 0.9% dry weight, respectively. Regarding those three species, there appeared a good source for bioactive substance and a new class of pharmaceutical preparation from its aboveground parts is being developed.