Chemical constituents and antitumor activity from stems and leaves of Dioscorea opposita / 中草药

Objective: To investigate the chemical constituents from the stems and leaves of Dioscorea opposita. Methods: The compounds were isolated and purified by various column chromatographies, and their structures were identified by physiochemical properties and spectroscopic data. The effects of the compounds on the proliferation of human breast cancer MCF-7 cells and human liver cancer HepG2 cells were investigated. Results: Twenty compounds were isolated from the 50% acetone extract of the stems and leaves of D. opposita, which were identified as 1H-indazole (1), 1H-indole-3-carboxaldehyde (2), 1H-indole-3- carboxylic acid (3), 3-(2-oxopropyl)-3-hydroxy-indolin-2-one (4), thymidine (5), uridine (6), 3-hydroxy-3-(2-oxopropyl)-2,4-(1H,3H)- quinolinedione (7), hematinic acid (8), allantoin (9), 2-ethyl-3-methyl-maleimide-N-β-D-glucopyranoside (10), paulownin (11), (+)-8-hydroxypinoresinol (12), (+)-syringaresinol (13), (-)-dihydrodehydrodiconiferyl alcohol (14), (7R,8S)-dihydrodehydro- diconiferyl alcohol-4-O-β-D-glucopyranoside (15), (2E,6S)-6,7-dihydroxy-3,7-dimethyl-2-octenoic acid (16), (2E,4S)-4-hydroxy-2- nonenoic acid (17), (2E,6S)-6-hydroxy-2,6-dimethyl-2,7-dienoic acid (18), amarantholidoside IV (19), (9Z,11E)-13-methoxy- 9,11-octadecadienoic acid methyl ester (20), and their effects on proliferation of MCF-7 cells and HepG2 cells were investigated. compounds 1, 4, 8, 11-15, 18, 20 at a dose of 25μmol/L inhibited the proliferation of MCF-7 cells, and compounds 1, 4, 8, 12-14, 18, 20 at a dose of 25 μmol/L inhibited the proliferation of HepG2 cells. Conclusion: Compounds 1, 3-5, 10-12 and 15-20 are isolated from this plant for the first time, compounds 1, 4,8, 11-15, 18, 20 inhibited the the proliferation of MCF-7 and HepG2 cellssignificantly at certain concentration, showing protent antitumor activity.

Study on lignans compounds from Lavandula angustifolia / 中草药

Objective: To investigate the lignans compounds constituents of Lavandula angustifolia. Methods: The chemical constituents were isolated and purified by TLC, silica gel, MCI-gel, and RP-HPLC, and their structures were identified by analysis of spectroscopic evidences and physicochemical properties. Results: A total of 11 constituents were isolated from L. angustifolia and elucidated as pinoresinol (1), syringaresinol (2), fraxiresinol-4'-O-β-D-glucopyranoside (3), syringaresinol-4'-O-β-D-glucopyranoside (4), 8-hydroxypinoresinol-4-O-β-D-glucopyranoside (5), rel-(2α,3β)-7-O-methylcedrusin (6), lariciresinol-4'-O-β-D-glucoside (7), (2S,3R)-2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxybenzofuran-5-(trans) propen-1-ol-3-O-β-glucoside (8), (7S,8R)-dihydrodehydrodiconiferyl alcohol-9-β-D-glucopyranoside (9), (7R,8R)-7,8-dihydro-9'-hydroxyl-3'-methoxyl-8- hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-1'-benzofuranpropanol-9'-O-β-D-glucopyranoside (10), and (E)-3-((2S,3S)-2-(4- hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxy-2,3-dihydrobenzofuran-5-yl) allyl-2-hydroxyacetate (11). Conclusion: The 11 compounds are isolated from this plant for first time.

AnewlignanfromstemsofTrigonostemonlutescens / 中草药

Objective: To study the chemical constituents in the stems of Trigonostemonlutescens. Methods:Silica gel column chromatography and semi-preparative high performance liquid chromatography were used for separation and purification. The structures were identified by NMR, IR, MS and other spectral techniques. Toxicity of compounds 1-4 on human lung cancer A549 cells, cervical cancer Hela cells and breast cancer MCF-7 cells was determined by the MTT method. Results: Four lignans were isolated from the stems of T. lutescens. The structures were identified as 5,5'-dimethoxylclemaphenol A (1), syringaresinol (2), lirioresinol B dimethyl ether (3) and clemaphenol A (4). Conclusion:Compound1 is a new compound, and compounds 2-4 are isolated from this genus for the first time. Compounds 1-4 have certain cytotoxic activity on A549, HeLa and MCF-7 human tumor cells.

Protective Effect of Syringaresinol on Excitatory Damage Induced by Sodium Glutamate in SH-SY5Y Cells / 中国实验方剂学杂志

Objective: To establish a model for the injury of human neuroblastoma cell (SH-SY5Y) induced by sodium glutamate, and to observe the protective effect of syringaresinol on cell damage from Viscum liquidambaricolum hayataon, and to explore its mechanism. Method: Construction of SH-SY5Y cell injury model using sodium glutamate.The experiment was divided into normal cell group, injury model group (sodium glutamate 50 mmol·L-1, sodium glutamate 50 mmol·L-1 + DMSO),syringaresinol experimental group (6.25, 12.5, 25 μmol·L-1), by cell counting, cell morphology observation, Annexin V-FITC/PI apoptosis detection, ROS reactive oxygen species detection, mitochondrial membrane potential, and Western blot, evaluation of syringaresinol on glutamate-induced neuronal excitability injury neuroprotective activity. Result: Compared with normal group, the cell survival rate of the model group was significantly decreased (PPPPP-1) showed a concentration-dependent increase in cells. Survival rate (PPPPPConclusion: Syringaresinol has significant protective activity against excitatory damage induced by sodium glutamate in SH-SY5Y neurons, the mechanism may be through anti-oxidative stress, repairing mitochondrial function and DNA damage to significantly reduce sodium glutamate-induced neuronal apoptosis.

Chemical constituents from rhizomes of Cimicifuga dahurica / 中草药

To isolate and identify the chemical constituents from the rhizome of Cimicifuga dahurica. Methods The isolation and purification of 60% EtOH extract of the rhizomes of C. dahurica were carried out through various modern chromatographic separation techniques: HP-20, silica gel, ODS, Sephadex LH-20 column and semi-preparative HPLC. And the structures of the compounds were identified based on spectroscopic data and physicochemical properties. Results Twenty compounds were isolated and identified as cimicifugaside F (1), (+) (2S,3R)-2-(4-hydroxy-3-methoxyphenyl)-3-[(β-D-glucopyranosyloxy) methyl]-7-methoxybenzofuran-5-propenoic acid (2), 5-hydroxy-2-methoxybenzoic acid (3), benzoic acid 4-O-β-D-glucoside (4), isoferulic acid (5), ferulic acid (6), trans-ferulic acid 4-O-β-D-allopyranoside (7), trans-ferulic acid 4-O-β-D-glucoside (8), (E)-sinapic acid 4-O-β-D-glucoside (9), 6,6’-di-O-sinapoylsurcose (10), piscidic acid (11), fukinolic acid (12), N-trans-feruloyltyramine 4-O-β-D-allopyranoside (13), N-trans-3’-methoxy-4’-feruloyltyramine-4-O-β-D-allopyranoside (14), N-trans-3’-methoxy-4’- feruloyltyramine-4-O-β-D-glucoside (15), grevilloside G (16), (-)-syringaresinol (17), (-)-syringaresinol 4,4’-di-O-β-D- allopyranoside (18), (+)-isolarisiresinol 3a-O-β-D-glucoside (19), (-)-5’-methoxyisolariciresinol 3a-O-β-D-glucoside (20). Conclusion Compound 1 was identified as a new lignan, and compounds 2-4, 8-10, 15-17 and 20 were isolated from Cimicifuga genus for the first time.

In vitro antileishmanial and antitrypanosomal activity of compounds isolated from the roots of Zanthoxylum tingoassuiba

Abstract Five coumarins (5,7,8-trimethoxycoumarin (1), sabandin (2), cubreuva lactone (3), 5,7-dimethoxycoumarin (5) and braylin (6)), seven furoquinoline alkaloids (isopimpinelin (4), pteleine (7), maculine (8), skimianine (10), robustine (11), y-fagarine (12) and dictamine (13) and the furofuran type lignin syringaresinol (9)) have been identified for the first time in the roots of Zanthoxylum tingoassuiba A. St.-Hil., Rutaceae. Pure compounds 1, 6, 9, 12 were tested against Leishmania amazonensis parasites and epimastigotes forms of Trypanosoma cruzi. All the tested products displayed an antiparasitic activity similar to that of the positive controls (benznidazole and amphotericin B). Compound 9 was the most active against both parasites with IC50 values of 11.98 µM and 7.55 µM against L. amazonensis and T. cruzi, respectively.

Chemical constituents from noni enzyme and their antitumor activities / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Noni enzyme (Morinda citrifolia L.) and their antitumor activities. METHODS: Compounds were isolated by various chromatographic techniques, including silica gel, TLC, sephadex LH-20, and semi-preparative HPLC, and their structures were identified by their physicochemical properties and 1H-NMR and 13C-NMR data. The in vitro antitumor activities of the isolated compounds were studied by MTT method. RESULTS: Sixteen compounds were isolated from Noni enzyme. They were xylogranatinin(1), pelargonic acid(2), 1,5,15-tri-O-methylmorindol(3), sesquipinsapol B(4), (+)-syringaresinol(5), pinonesinol(6), 3-methylhexahydropyrrolopyrazine-1,4-dione(7), (2S)-3′-hydroxybutan-2-yl-2-hydroxypropanoate (8), 3-(sec-butyl)-6-methylpiperazine-2,5-dione(9), cyclo-(L-Pro-L-Leu)(10), gentisic acid(11), vomifoliol(12), scopoletin(13), 3-(2-hydroxy-4,5-dimethoxyphenyl) propanoic acid(14), medioresinol(15), hydroxychavicol(16). CONCLUSION: Compounds 1-10, 12 and 14-16 are isolated from Noni enzyme for the first time. Compound 10 displays the stronger cytotoxicity against HepG2 and HeLa cells with an IC50 value of 23.73, 16.55 μg•mL-1. Compound 5 had a certain inhibitory activity against HeLa cells with an IC50 value of 47.12 μg•mL-1.

Lignans chemical constituents from Diaphragma Juglandis Fructus and activity of inhibiting HIV / 中草药

Objective To study the lignans chemical constituents from Diaphragma Juglandis Fructus and their activity of inhibiting HIV. Methods The constituents were isolated from Diaphragma Juglandis Fructus and purified by column chromatography, and the structures were identified by spectra analysis and chemical methods.The activity of anti-HIV-1 were detected by LEDGF/p75-IN proteins complex ELISA kit. Results Sixteen compounds were isolated from Diaphragma Juglandis Fructus and the structures were identified as (-)-syringaresinol (1), (+)-pinoresinol (2), (+)-(7R,7’R,7″S,7’’’S,8S,8’S,8″S,8’’’S)-4″,4’’’-dihydroxy-3,3’,3″,3’’’,5,5’- hexamethoxy-7,9’;7’,9-diepoxy-4,8″;4’,8’’’-bisoxy-8,8’-dineolignan-7″,7’’’,9″,9’’’-tetraol (3), 2,3-dihydroxy-1-(4’-hydroxy-3’-methoxy- phenyl)-propan-1-one (4), 3-hydroxy-1-(4’-hydroxy-3’-methoxyphenyl)-propan-1-one (5), 3’,4’-dimethoxyphenylpropanediol (6), (2S)-3,3-di-(4-hydroxy-3-methoxyphenyl)-propane-1,2-diol (7), 3-hydroxy-1-(4’-hydroxy-3’,5’-dimethoxy phenyl)-propan-1-one (8), (1R,5R,6R)-6-{4’-O-[8″-(7″-(4″-hydroxy-3″-methoxyphenyl)) glyceol]-3’,5’-dimethoxyphenyl}-3,7-dioxabicyclo [3.3.0] octan-2-one (9), curcasinlignan B (10), evofolin-B (11), (7S,8R)-dihydrodehydrodiconiferyl alcohol (12), pinnatifidanin C I (13), (+)-(7S,8S)-4,1’-dihydroxy-3,3’,5’-trimethoxy-7,8,9-trinor-8,4’-oxyneolignan-7,9-diol (14), dysosmarol (15), and 1-(4’-hydroxy-3’-methoxyphenyl)-2- [4″-(3-hydroxypropyl)-2″,6″-dimethoxyphenoxy]-propane-1,3-diol (16). Conclusion All compounds are isolated from Diaphragma Juglandis Fructus for the first time. Compound 13 has the potential activity of inhibiting HIV-1.

Lignans from stems of Clausena emarginata / 中草药

Objective To investigate the lignans from the stems of Clausena emarginata. Methods The compounds were isolated and purified by chromatography on kieselguhr, silica gel, MPLC, and preparative HPLC. Their structures were identified on the basis of spectral data and physicochemical properties. Inhibitory activities on LPS-induced NO production of the lignans were initially investigated. Results Sixteen lignans were isolated from the CHCl3 fractions of 95% ethanol extract of the stems of C. emarginata, and their structures were identified as buddlenol C (1), hedyotol D (2), hedyotol C (3), 3-(2,4-dihydroxy-3-methoxybenzyl)-4- (4-hydroxy-3-methoxybenzyl) tetrahydrofuran (4), tripterygiol (5), busaliol (6), 2,3-bis [(4-hydroxy-3,5-dimethoxyphenyl)-methyl]- 1,4-butanediol (7), polystachyol (8), syringaresinol (9), nitidanin (10), 4-[3-hydroxymethyl-5-((E)-3-hydroxypropenyl)-7-methoxy- 2,3-dihydrobenzofuran-2-yl]-2,6-dimethoxy-phenol (11), erythro-guaiacylglycerol-β-O-4’-sinapyl ether (12), erythro-guaiacylglycerol- 8-O-4’-(coniferyl alcohol) ether (13), erythro-1-(4-hydroxy-3-methoxyphenyl)-2-(4-formyl-2-methoxyphenoxy)-propane-1,3-diol (14), rosalaevin B (15), and dehydroconiferyl alcohol (16). Conclusion Compounds 1-15 are isolated from this plant for the first time. Compounds 8 and 16 show inhibitory effects against LPS-induced NO production in microglia BV2 Cell.

Chemical constituents from ethyl acetate extract of Gleditsiae Spina / 中草药

Objective To investigate the chemical constituents from the ethyl acetate extract of Gleditsiae Spina. Methods The chemical constituents were isolated and identified by chromatography on silica gel, Toyopearl HW-40C, Sephadex LH-20, MCI Gel CHP-20, ODS, and RP-HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral analyses. Results Eighteen compounds were isolated from the ethyl acetate extract of Gleditsiae Spina, and identified as scopoletin (1), (-)-(7R,8S)-erythro-guaiacylglycerol (2), 5-methoxy-guaiacylglycerol (3), (-)-(7R,8R)-threo-guaiacylglycerol (4), 3,4’- dihydroxypropiophenone (5), dihydroferulic acid (6), p-hydroxybenzoic acid (7), indole-3-carbaldehyde (8), protocatechuic acid methyl (9), protocatechuic aldehyde (10), syringic acid (11), 2-guaiacylpropane-1,3-diol (12), (2R*,3R*,4S*)-2,3-diguaiacyl-4- hydroxyl tetrahydrofuran (13), 3-(2-oxopropyl)-3-hydroxy-indolin-2-one (14), juglanin D (15), C-veratroylglycol (16), 3-(4-hydroxyl-3-methoxyphenyl)-propan-1,2-diol (17), and (-)-syringaresinol (18). Conclusion Eighteen compounds are isolated from this plant for the first time.

Chemical constituents from aerial parts of Leonurus macranthus / 中草药

Objective: To investigate the chemical constituents from the aerial parts of Leonurus macranthus. Methods: The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and semi-preparative HPLC. Their structures were determined by physicochemical properties and spectroscopic data. Results: Nineteen compounds were isolated from the CH2Cl2 layer of 70% aqueous acetone extract in the aerial parts of L. macranthus, and identified as (+)-syringaresinol (1), (+)-1-hydroxysyringaresinol (2), rayalinol (3), erythro-guaiacylglycerol-β-O-4'-coniferyl ether (4), (7R, 7'R, 7″S, 8S, 8'S, 8″S)-3', 4″- dihydroxy-3, 5, 4', 5″-tetramethoxy-7, 9': 7', 9-diepoxy-4, 8″-oxy-8, 8'-sesquineo-lignan-7″, 9″-diol (5), (7R, 7'R, 7″S, 8S, 8'S, 8″S)-4', 5″- dihydroxy-3, 5, 3', 4″-tetramethoxy-7, 9': 7', 9-diepoxy-4, 8″-oxy-8, 8'-sesquineo-lignan-7″, 9″-diol (6), genkwanin (7), 3'-hydroxy- genkwanin (8), eriodictyol (9), isoscopoletin (10), p-coumaric acid (11), caffeic acid methyl ester (12), trans-ferulic acid (13), syringic aldehyde (14), vanillic acid (15), oct-1-en-3-yl β-glucopyranoside (16), 5-hydroxy-2-pyrrolidone (17), pterolactam (18), and nicotinamide (19), respectively. Conclusion: Compounds 1-6 and 9-19 are isolated from the plants of genus Leonurus Linn. for the first time, and compounds 7 and 8 are found from L. macranthus for the first time.

Syringaresinol-4---d-glucoside alters lipid and glucose metabolism in HepG2 cells and C2C12 myotubes

Syringaresinol-4---d-glucoside (SSG), a furofuran-type lignan, was found to modulate lipid and glucose metabolism through an activity screen of lipid accumulation and glucose consumption, and was therefore considered as a promising candidate for the prevention and treatment of metabolic disorder, especially in lipid and glucose metabolic homeostasis. In this study, the effects of SSG on lipogenesis and glucose consumption in HepG2 cells and C2C12 myotubes were further investigated. Treatment with SSG significantly inhibited lipid accumulation by oil red O staining and reduced the intracellular contents of total lipid, cholesterol and triglyceride in HepG2 cells. No effect was observed on cell viability in the MTT assay at concentrations of 0.1-10 μmol/L. SSG also increased glucose consumption by HepG2 cells and glucose uptake by C2C12 myotubes. Furthermore, real-time quantitative PCR revealed that the beneficial effects were associated with the down-regulation of sterol regulatory element-binding proteins-1c, -2 (), fatty acid synthase (), acetyl CoA carboxylase () and hydroxyl methylglutaryl CoA reductase (), and up-regulation of peroxisome proliferator-activated receptors alpha and gamma (and). SSG also significantly elevated transcription activity oftested by luciferase assay. These results suggest that SSG is an effective regulator of lipogenesis and glucose consumption and might be a candidate for further research in the prevention and treatment of lipid and glucose metabolic diseases.

Chemical constituents from aerial part of Polygonatum cyrtonema / 中草药

Objective: To study the chemical constituents from the aerial part of Polygonatum cyrtonema. Methods: The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Results: Fifteen known compounds were isolated in methanol extract from the aerial part of P. cyrtonema and their structures were identified as (3R)-5,7-dihydroxy-3-(2'-hydroxy-4'-methoxybenzyl)-chroman-4-one (1), 5,7-dihydroxy-6-methyl-3-(2',4'-dihydroxybenzyl)- chroman-4-one (2), 5,7-dihydroxy-6-methyl-3-(4'-hydroxybenzyl)-chroman-4-one (3), (3S)-3,7-dihydroxy-8-methoxy-3-(3',4'- methylenedioxybenzyl)-chroman-4-one (4), apigenin (5), kaempferol (6), vanillic acid (7), trans-p-hydroxycinnamic acid (8), trans-p-hydroxycinnamic acid methyl ester (9), saliylic acid (10), (+)-syringaresinol (11), balanophonin B (12), caffeic acid (13), phenylalanine (14), and coniferaldehyde (15). Conclusion: Compounds 1, 2, 7, 11, 14, and 15 are firstly obtained from P. cyrtonema. Compounds 3, 4, and 12 are isolated from the plants of Polygonatum Mill. for the first time.

Chemical Constituents of Ixora philippinensis Merr.

Chemical investigations of the dichloromethane extracts of Ixora philippinensis Merr., a plant endemic to the Philippines, led to the isolation of syringaresinol (1), pinoresinol (2), isoscopoletin (3), squalene (4), β-sitosterol (5a), and stigmasterol (5b) from the stems; and 4, 5a, 5b, lupeol (6), and lutein (7) from the leaves. The structures of 1 and 3 were elucidated by extensive 1D and 2D NMR spectroscopy, while those of 2 and 4-7 were identified by comparison of their NMR data with literature data.

In vitro metabolic transformation of syringin by rat intestinal flora / 中草药

Objective: To study the in vitro metabolic transformation of syringin by rat intestinal flora in vitro. Methods: When syringin was incubated for 0, 4, 8, 12, 24, and 48 h with rat intestinal flora in vitro, the incubation medium was respectively extracted with n-BuOH, and then HPLC and LC-MS were applied for the qualitative and quantitative analysis of the metabolites. The metabolites were extracted with n-BuOH, isolated by ODS column chromatography, and recrystallization from the extended medium after 24 h of incubation, and their structures were determined on the basis of the NMR experiments. Results: Syringin could be easily metabolized by rat intestinal flora in vitro. About 81% of the syringin was metabolized at 12 h, and it was completely metabolized at 24 h. Two metabolites, sinapyl alcohol (M1) and (+)-syringaresinol (M2), in the incubation medium of experimental group were identified within 48 h. The major metabolite was M1 during the first 12 h, and M2 was the only metabolite identified after 24 h. Conclusion: In vitro, syringin could be completely metabolized to M1, and then converted into M2 by rat intestinal flora within 24 h, and the metabolism of syringin to M1 is proceeded most rapidly in 8~12 h.

Chemical constituents of Aganosma marginata / 中草药

Objective: To investigate the chemical constituents from Aganosma marginata and to provide the material basis for the quality control. Methods: The chemical constituents were separated and purified by silica gel Sephadex LH-20, MCI. Their structures were determined by physicochemical properties and spectral data analyses. Results: Twenty three compounds were isolated from A. marginata and identified as: periseoside C (1), 3-O-β-D-glucopyranosyl-3β,15α-dihydroxypregn-5-en-20-one (2), 3β,20α-dihydroxy- 5-en-pregane (3), 28-methylstigmast-5-en-3-ol (4), 29-norcycloart-23-ene-3,25-diol (5), 29-norcycloartan-3-ol (6), geniposidic acid (7), syringaresinol (8), syringaresinol-4,4'-O-bis-β-D-glucopyranoside (9), syringic acid 4-O-β-D-glucopyranoside (10), salicylic acid (11), scopoletin (12), azelaic acid (13), 3-O-[β-D-xylopyranosyl]-(1→4)-β-D-allopyranoside-14-hydroxycard-20 (22)-enolide (14), bis (2-ethylhexyl) phthalate (15), kaempferol-3-O-α-L-rhamnopyranosyl-(l→4)-α-L-glucopyranoside (16), kaempferol-3-O-α-L- glucopyranoside-(1→2)-β-D-glucopyranoside (17), kaempferol-3-O-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl (1→4)]-β- D-glucopyranoside (18), kaempferol-3-O-α-L-rhamnopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside (19), hexacosanoicacid 1-carbonate (20), 5,8,12-trihydroxy-9-octadecenoic acid (21), (2S,3S,4R)-phytosphingosine (22), and nebularine (23). Conclusion: All the compounds are obtained from plants of Aganosma G. Don for the first time.

Chemical constituents of stems of Acanthopanax senticosus / 中国药学杂志

OBJECTIVE: To study the chemical constituents in the stems of Acanthopanax senticosus from Changbai Mountain area.

Chemical constituents from Orobanche cernua / 中草药

Objective: To study the chemical constituents from Orobanche cernua in Orobanchaceae. Methods: The chemical constituents were separated and purified by macroporous resin, silica gel, Sephadex LH-20, and MCI column chromatographies. Their structures were determined by physicochemical properties and spectral data. Results: Twelve compounds were isolated from 70% ethanol extract of O. cernua. Among them, eight phenylpropanoid glycosides were identified as acteoside (1), campneoside II (2), crenatoside (3), campneoside I (4), isocrenatoside (5), isoacteoside (6), leucosceptoside A (7), and cistanoside F (8), three liganans were identified as (+)-syringaresinol-4'-O-β-D-glucopyranoside (9), (+)-pinoresinol-4'-O-β-D-glucopyranoside (10), and isoeucommin A (11), and one steroide was stigmasterol-3-O-β-D-glucoside (12). Conclusion: Compounds 7 and 12 are isolated from the family Orobanchaceae for the first time; Compounds 4, 7, and 9-12 are isolated from the plants of Orobanche L. firstly, and compounds 2, 4, and 6-12 are found from O. cernua for the first time.

Low-polarity chemical constituents from Scrophularia dentata grown in Tibet / 中草药

Objective: To study the low-polarity chemical constituents from the whole plants of Scrophularia dentata grown in Tibet. Methods: The compounds were isolated and purified by various column chromatography. Their structures were elucidated by means of spectral analyses (MS, 1H-NMR, and 13C-NMR). Results: Fifteen compounds were isolated and identified as oleanolic acid (1), 3-O-acetyloleanolic acid (2), stigmast-4-en-3-one (3), stigmast-4, 22-dien-3-one (4), 2, 6-dimethoxybenzoquinone (5), cerevisterol (6), cinnamic acid (7), spinasterol (8), β-sitosterol (9), 2-tritriacontanone (10), 2-(4-hydroxyphen-yl)-ethyl 1-dodecyloctadecanoate (11), apigenin (12), chrysoeriol (13), medioresinol (14), and syringaresinol (15). Conclusion: All the compounds are firstly isolated from this plant and compounds 2-6 and 10-15 are isolated from the plants of this genus for the first time.

Chemical constituents of Whole Plant of Veronicastrum axillare (Sieb. et Zucc.) Yamazaki / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Whole Plant to the whole plant of Veronicastrum axillare(Sieb. et Zucc.)Yamazaki. METHODS: The compounds were isolated and purified by various chromatographic techniques, and their structures were identified on the basis of physicochemical properties and spectral analysis. RESULTS: Twenty-three compounds were identified as 5-hydroxy-7, 4'-dimethoxyflavone(1), acacetin(2), 5, 7, 4'-trihydroxy-8-methoxyflavone(3), 5, 7, 3'-trihydroxy-4'-methoxyflavone(4), luteolin(5), oleic acid(6), syringaresinol(7), glyceryl ferulate(8), cinnamic acid(9), p-hydroxycinnamic acid(10), caffic acid(11), ferulic acid(12), 3, 4-dimethoxycinnamic acid(13), p-hydroxybenzoic acid(14), protocatechuic acid(15), vanillic acid(16), isovanillic acid(17), protocatechuic aldehyde(18), hydroquinone(19), 1, 2, 4-benzenetriol(20), D-mannitol(21), sucrose(22), and β-sitosterol(23). CONCLUSION: All the compounds are isolated from this plant for the first time except for compounds 2, 5, 19, 21 and 23, and from the genus Veronicastrum for the first time except for compounds 2, 5, 6, 12, 13, 19, 21 and 23. The 13C-NMR data of compound 8 is reported for the first time.