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OBJECTIVE:To establish a dualwavelength TLC-scanning method for the determination of nucleosides in the preparation of Cordyceps sinensis.METHODS: The sample was analyzed on silica GF254 thin layer plate using 1% CMC-Na as adhesive and chloroform-ethylacetate-isopropanol-water-ammonia(8∶2∶6∶0.5∶0.12) as developing agent and detected by dualwavelength reflection sawtooth scanning(?s=254 nm and ?R=300 nm).RESULTS:Adenosine,Uracil and Uridnine had good linearity in the ranges of 11.16～58.03 ?g(r= 0.998 5),1.04～6.24 ?g(r=0.991 5) and 0.8～4.8 ?g(r=0.991 2) respectively,and their average recovery rates were 95.559%,96.366% and 95.270% and RSD were 0.74%,0.73% and 0.98% respectively(n=6).CONCLUSION: The method is simple,accurate,reproducible,and can be used as the quality control of Cordyceps sinensis preparation.
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Objective To select the best condition of preparation process for the water-extraction and alcohol-precipitation method of Radix astragali in “Leifengguan” granules. Methods The effective compound astragaloside IV of Radix astragali was determined with the methods of TLC-scanning. The preparation process was screened with orthogonal design [L9(34)] and single factor analysis. Results The best condition of preparation process for water-extraction of Radix astragali was A3B2C2 that the drug materials were decocted for 3 times with 8 times amount of water, each time for 1 hour. After the solution was concentrated to proportion as 1 g/mL (drug materials/solution), alcohol was added to the solution to 60% alcohol. Conclusions The optimized preparation process was found to be stable with a good reproducibility.
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Objective To establish quality standard for Liangsang Diandiye. Method Radix et Rhizoma Notoginseng, Flos Carthami and Radix Salviae Miltiorrhiizae were identified by TLC. Ginsenoside Rg1 was determined by dual-wavelength TLC-scanning with 525 nm and 700 nm as the detective wavelength. Results Radix et Rhizoma Notoginseng, Flos Carthami and Radix Salviae Miltiorrhiizae can be identified by TLC. A good line relationship was noted at the range of 1.53～7.65 ?g Ginsenoside Rg1 (r =0.9991). The average recovery was 99.10%, and RSD was 0.27%. Conclusion The established method can be used for the quality control of Liangsang Diandiye.
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Objective To establish a method for determination of chrysophanol,emodin and rhein in Huangzhi capsules.Method Chrysophanol,emodin and rhein in Huangzhi capsules was determined by TLC-scanning with a mixture of n-hexane-ethylacetate-formic acid(30∶10∶0.5) as the developing system.The detection wavelength was 435 nm and 610 nm.Results The calibraction curve of chrysophanol,emodin and rhein was linear in the range of 0.014 65～0.073 25,0.013 35～0.066 75,0.012～0.072 ?g respectively.Conclusion The method was simple,accurate with good repeatability,and suitable for the content determination of chrysophanol,emodin and rhein in Huangzhi capsules.
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OBJECTIVE:To establish TLC scanner method for the determination of stachydrine hydrochloride in xue?niaokang granule.METHODS:Silica gel G thin layer plate was adopted in the determination with n-butanol-hydrochloric acid-ethyl acetate(8∶3∶1)used as developer and thin potassium heptaiodobismuthate test solution used as color-developing agent,the detection wavelength was515nm,the reference wavelength was700nm and the slit size was6.00mm?0.45mm.RESULTS:Good linear correlation with the peak area score of spots achieved when the concentration range of stachydrine hydrochloride was within5.06?g～25.32?g(r=0.9978),the average recovery was96.67%(RSD=2.01%).CONCLUSION:The method was accurate,simple,reliable,sensitive and reproducible,and it can be used for the determination of xueniaokang granule and its quality control.
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OBJECTIVE:To establish the quality control method for compound seabuckthorn oil granule.METHODS:Radix astragali,angelica root,rhizoma chuanxiong,seabuckthorn seed oil were identified by TLC and the content of astragalosideⅠin compound seabuckthorn oil granule was determined by TLC-scanning method.RESULTS:Radix astragali,angelica root,rhizoma chuanxiong,seabuckthorn seed oil could be detected by TLC.Good liner relationship achieved when the sample application size of astregalosideⅠwas with the range of 0.98?g～4.90?g,the average recovery was 99.62%(RSD=2.51%).CONCLUSION:This method is simple,accurate with good reproducibility and strong specificity,and it can be used for the quality control of compound seabuckthorn oil granule.
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OBJECTIVE:To establish a method for the determination of ursolic acid in pedo-stomach-invigorating&digestion-promoting granule.METHODS:The determination was performed by TLC-scanning method in which chloro-form-acetone-glacial acetic acid(18∶2∶0.2)was used as developing solvent,and10%sulphuric acid ethanol solution was taken as developer,which were baked under110℃until limpid prunosus spots were occurred,the scanning method was dual wavelength reflection zigzag scanning with detection wavelength at520nm,reference wavelength at700nm,linearity parameter Sx=3and slit size at0.4mm?0.4mm.RESULTS:Ursolic acid has a good linear relation with peak area score when its sample size was within the range of2?g～10?g(r=0.9996),the average recovery is97.8%(RSD=1.4%).CONCLUSION:This me_ thod can be served as a quality control for pedo-stomach-invigorating&digestion-promoting granule.
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OBJECTIVE:To determine the content of the total flavonoid in Fructus Rosae Laevigatae.METHODS:Dual wavelength TLC scanner was selected with rutin as standard substance.The developing solvent was acetic ether-formic acid-water(8∶1∶1);?s=266 nm,and ?R=230 nm.RESULTS:The linear range of Fructus Rosae Laevigatae was 1～6 ?g,and the average recovery was 98.9% 2.397%(RSD=1.39%,n=6).CONCLUSION:The method is simple and accurate,and it is applicable for the quality control of Fructus Rosae Laevigatae.
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OBJECTIVE:To establish determine the content of berberine hydrochloride in the pessums.METHODS:Ben-zene-ethyl acetate-isopropanol-methanol-water(6∶3∶1.5∶1.5∶3)were taken as developers,which were detected under the UV light(365nm)and scanned by TLC scanner with the detective wavelength at430nm.RESULTS:The linear range of berberine hydrochloride on calibration curve was sample size0.25?g～1.25?g(r=0.9987),the average recovery rate was97.63%(RSD=1.88%).CONCLUSIONS:The method is simple,accurate and available for the quality control of pessums.
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OBJECTIVE:To prepare Gongjinkang Effervescent Tablets and to establish a method of quality control for this tablets.METHODS:The Gongjinkang Effervescent Tablets was prepared with mixture of purified powdered extract from6kinds of traditional Chinese medicine such as Flos Mume and Borneol,Sodium bicarbonate,tartaric acid,etc.;the Flos Mume,Phellodendron and borneol were identified by the TLC;the content of berberine hydrochloride was determined by TLC-scanning.RESULTS:The TLC chraomatogram spots were clear and no interference was found in the negative reference;the linear range of berberine hydrochloride on calibration curve was0.174?g～1.044?g(r=0.9995),the average recovery was98.01%～100.80%and RSD
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OBJECTIVE:To determine the contents of hydrochloric stachydrine in the shenning granule by TLC-scanning Method.METHODS:The acetone-dehydrated alcohol-HCl(10∶6∶1)were used as developing agents,the potassium hep-taiodobismuthate test solution-1%trichloride ferric dehydrated alchhol solution(2∶1)were used as the color-developing a-gents,the detection wavelength was530nm,the reference wavelength was670nm,the slit size was0.4mm?0.4mm,the lin-earization parameter Sx=3.RESULTS:The spot peak area score assumed a satisfactory linear relationship when the electric sample size for the hydrochloric stachydrine at a range of2.412?g～6.432?g(r=0.9972),the recovery was98.93%(RSD=1.15%).CONCLUSION:This method is simple,accurate,and this determination method has a good reproducibility,which can be used for the quality control of the shenning granule.
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OBJECTIVE:To optimize the extraction craft of gentiopicrin from gentian.METHODS:The extraction craft was optimized by orthogonal test with gentiopicrin as an index,and the content of gentiopicroside was determined by TLC scanning method.RESTLUTS:The quantity of menstruum and the extraction times of gentiopicrin had significant influence in the gentiopicrin extraction(P
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OBJECTIVE:To establish the standard for quality control of Bushenxiaoshi granules.METHODS:TLC was applied to identify the properties of Rhizoma Corydalis,Rhizoma Alismatis and Rhizoma Dioscoreae;TLC scanning method was applied to determine the content of Tetrahydropalmatine in Bushenxiaoshi granules.RESULTS:The three herbs could be identified by TLC.There was a good linear relationship with Tetrahydropalmatine at the range of 0.408～ 2.04? g(r=0.997 5),and the average recovery is 95.94%(RSD=1.85%).CONCLUSION:The methods could be used for quality control of Bushenxiaoshi granules.
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Objective To establish a quality control method for tanshinone ⅡA in Niaotong Tablets.Methods The content of tanshinone ⅡA was determined by TLC-scanning method. Benzene-ethyl acetate(19 ∶1) was used as the developer, ?s = 470nm,?R = 620nm,SX=3.Results A linearity was obtained from 0.44?g to 2.20 ?g of tanshinone ⅡA in Niaotong Tablets (r=0.9993,n=5);the average recovery rate was 98.08 %,RSD=3.29 %.Conclusion This method is simple, sensitive and reproducible for the determination of tanshinone ⅡA in Niaotong Tablets and Radix Salviae Miltiorrhizae.
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OBJECTIVE: Simulateous determination of ten psychosedatives in serum was made to help doctors make effective rescue. METHOD: The qualitative separation was done by TLC. The quantitative determination was made by TLC scanning. RESULTS: Ten psychosedatives were separated very well by one mobile phase. The recovery, accuracy and stability of TLC scanning on varied wave-lengths were satisfatory. CONCLUSION: This method is simple, rapid, and accurate. It can be used in poison analysis and therapeutic drug monitoring.
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Object To investigate the effects of different processed on content of matrine in the root of Sophora tonkinensis Gapnep. Methods The determination of matrine was carried out by double wavelength TLC scanning for different processed products. Results There were obviously differences between the crude and kinds of different processed products of matrine in the root of S. tonkinensis. Conclusion The different processing methods and adjuvant materials can make the contents of matrine in the root of S. tonkinensis change before and after processing.
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Objective To establish a determination method for dehydrocostus lactone in Dahuolu Pill.Methods TLC-Scanning was used.Results The average recovery was 98.30 %and RSD was 1.90 %.Conlusion The method is simple,accurate,repeatable,and can be used for the reference of the quality control to Dahuoluo Pill.
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The dual wavelength TLC-scanning method was used to determine the content of ephedrine and amygdalin in Sanao Decoction. The average recovery of both ephedrine and amygdalin are 99.51％ and 98.40％, respectively.
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The content of geniposide in Compound Zhizi Granules was determined With TLC-scanner. The optimum experiment design obtained with orthogonal test could eliminate the disturbance of other components. The result was satisfactory.
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Objective: To establish a quantitative method for determination of protocatechuic acid in Xueniao Capsules. Methods: The capsules were extracted with alcohol acetic acid (10∶1) and then solved with absolute alcohol. The sample was applied on a silica H plate, developed and separated in the solution of chloroform acetone methanol acetic acid (7∶2∶1.5∶0.5). The separated spots were determined by TLC scanning at 530nm(? s). Results: The average recovery was 101.4%. Conclusion: This method for determination of protocatechuic acid in Xueniao Capsules was sensitive, accurate and specific. It could be used as quality control for Xueniao Capsules.