ABSTRACT
Objective To investigate the effects of Liujing Toutong Tablets (LTT) on isolated vascular smooth muscle and the expression of calcitonin gene-related peptide (CGRP) in trigeminus. Methods Rats’ thoracic aorta and trigeminus were isolated to investigate the exact effect by measuring the contractile response of vascular and the expression of CGRP in trigeminus. Results The LTT could reduce the quantity of positive CGRP cells expressed in trigeminus (P < 0.05) and weaken the contractile response of thoracic aorta induced by KCl and NE, and the IC50 is 0.97 mg/mL and 0.55 mg/mL, respectively. Conclusion LTT could inhibit contractile response of vascular smooth muscle, meantime, reduce the quantity of positive CGRP cells expressed in trigeminus. The results show that LTT can stabilize vascular systaltic property.
ABSTRACT
Objective To investigate how SDF-1α and c-MYC protein regulates TACRl-Tr expression. Methods c-myc shRNA vector was constructed, small interfering RNA was employed for silencing c-myc gene in MCF-7 breast cancer cell. SDF-1α neutralized antibody was used in c-myc+ cell group and c-myc- cell group, while other c-myc+ cell group and c-myc- cells group were cultured under normal condition. The mRNA level of TACRl-Tr was determined by real-time PCR. Results c-myc shRNA vector was constructed successfully, in the normal presence of SDF-la, the level of TACRl-Tr mRNA in c-myc- cell group were lower than that in c-myc+ cell group( P < 0.05). But in the presence of SDF-la neutralized antibody, TACRl-Tr mRNA level of c-myc- cell group was higher than that of c-myc+ cell group(P < 0.05). Conclusion In the normal culture condition, c-MYC protein may transactivate TACRl-Tr transcription in MCF-7 cell, in the presence of SDF-1α neutral antibody, c-MYC protein lost the activity of transactivating for TACRl-Tr transciption.