ABSTRACT
OBJECTIVE@#To investigate the therapeutic effects of Hedyotis diffusa Willd.on type Ⅱ collagen-induced rheumatoid arthritis in rats.@*METHODS@#According to the random number table, 60 SD rats were divided into the normal control group (=10, normal saline) and model group (=50).The collagen-induced arthritis model was established with the injection of type Ⅱ collagen into the back in rats other than the normal group and evaluated by arthritis score, then the model rats were randomly divided into model group (normal saline), tripterygium wilfordii polyglycoside (GTW) 6 mg/kg group (daily dose:0.4 mg/kg), HD 3, 6, 12 g/kg groups (daily dose:3, 6 and 12 g/kg, respectively), with 10 rats in each group. The rats were treated with corresponding agents by intragastric administration.The arthritis index and the pain threshold of all rats at different time points were observed and measured weekly.After treated by intragastric administration for 28 days, all rats were killed to measure the changes of serum cytokine levels including interleukin 1β (IL-lβ), tumor necrosis factor a (TNF-a), prostaglandin (PGE), receptor activator for nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG).@*RESULTS@#Compared with the control group, the arthritis index and the serum levels of IL-lβ, TNF-a, PGE, RANKL, OPG and RANKL/OPG of the model group were increased significantly (<0.05), the pain threshold of the model group was decreased significantly (<0.05); compared with the model group, the arthritis index and the serum levels of IL-lβ, TNF-a, PGE, RANKL, OPG and RANKL/OPG of the GTW group, HD low-dose, medium-dose, high dose groups were decreased significantly (<0.05), the pain threshold of the model group was increased significantly (<0.05).@*CONCLUSIONS@#Hedyotis diffusa Willd.can significantly reduce arthritis index and increase pain threshold, reduce the level of IL-lβ, TNF-a, PGE, RANKL, OPG, and RANKL/OPG, then can prevent CIA effectively.
Subject(s)
Animals , Rats , Arthritis, Experimental , Arthritis, Rheumatoid , Collagen Type II , Hedyotis , RANK Ligand , Rats, Sprague-DawleyABSTRACT
Objective: To observe the therapeutical effect of Yishen Juanbi Pill (YJP) on type II collagen induced arthritis (CIA) in rats and the histopathologic changes in ankle joint and to explore its possible mechanism on rheumatoid arthritis (RA). Methods: The therapeutical effect of YJP on CIA rat paw swelling and the histopathologic morphology of the local tissue of ankle joint were observed by the type II CIA rat model. The expression of VEGF of the synovial membrane was assayed by the immunohistochemical method. The changes in VEGF gene expression of peripheral lymphocytes and hepatic tissue were analyzed with gene chip after the treatment with YJP. Results: The decreased swelling degrees of foot of rats in low-, mid-, and high-dose YJP groups were increased obviously than that of the model group (P 1.5). Conclusion: YJP can significantly inhibit joint swelling. YJP can significantly down-regulate the VEGF-B gene expression, thereby inhibit the formation of synovial tissue pannus and reduce cartilage and bone damages.
ABSTRACT
Objective To discuss effects of anti-inflammatory mechanism of amygdalin on rats with type II collagen-induced arthritis (CIA). Methods Wistar rats were randomized into normal group, model group, amygdalin group, and tripterygium group. Type II CIA rat models were established. From the 15th day after the modeling establishment, each administration group was given corresponding dose of medicine for continuous 28 days. Levels of TNF-αand sICAM-1 were detected by ELISA in serum of rats, and expression of TNF-α was detected by immuno-histochemical method. Results TNF-α positive expression in amygdalin group and tripterygium group was similar and significantly reduced compared with model group. Levels TNF-α and sICAM-1 in amygdalin group and tripterygium group significantly decreased compared with those in model group (P0.05). Conclusion Amygdalin can inhibit the expression of TNF-α and levels of TNF-α and sICAM-1, in order to treat rheumatoid arthritis.
ABSTRACT
Aim: To study the therapeutic effects of recombinant human interleukin 1 receptor antagonist (rhIL-1ra) on type II collagen-induced arthritis (CIA) rats. Methods: SD rats were divided randomly into six groups including normal, model, rhIL-1ra (7.5,30,120 mg·kg-1) and anakinra(120 mg· kg-1) groups. Collagen II emulsion was used to induce CIA model in rats. The body weight was observed once a week. Paw swelling of CIA rats was measured with volume meter. C II induced delayed-type hypersensitivity (DTH) was measured. Meanwhile, the level of anti-C II IgG antibody in serum was assayed by ELISA. Results: The onset of paw swelling was on dlO after injection of emulsion. The level of serum anti-C II IgG antibody was increased significantly in CIA. Pathological changes in joints of CIA rats showed hyperplastic synovium of CIA, inflammatory cells infiltration, pannus, destruction of cartilage and bone. rhIL-1ra(7.5,30,120 mg·kg-1·d-1 x 7 d) and anakinra (120 mg·kg-1·d-1 x 7 d) subcutaneous injection (sc) inhibited inflammatory swelling. rhIL-1ra(30, 120 mg·kg-1·d-1 x 7 d) significantly suppressed the DTH reaction induced with C II in CIA rats. Moreover, rhIL-1ra reduced the level of anti-C II IgG antibody in serum. Pathological examination showed rhIL-1ra(120 mg·kg-1·d-1 x 7 d) significantly improved subchondral inflammation, synovium hyperplasia, pannus and cartilage damage. Conclusion: rhIL-1ra has therapeutic effects on CIA rats.
ABSTRACT
PURPOSE: Rat arthritis was induced by bovine type II collagen. To present the remedial effects on type II collagen arthritis and clinical efficacy, a high dose of methotrexate, of a level similar to that used in cancer patients was given to rats. MATERIALS AND METHODS: Healthy male Brown Norway rats were used, of mean weight; 300 g. There were 7 subgroups: a control group, given collagen only, an orally methotrexate treated (2.5mg/week), high methotrexate dose groups; 10 mg/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg and a low dose methotrexate group (0.375 mg/kg/week, 4 times) were included in the present study. Type II collagen was injected for arthritis induction and a single intraperitoneal injection of methotrexate was administered after 15 days. Orally treated group was administered 4 times (2.5 mg/week). Histopathologic findings were evaluated. RESULTS: 50 mg/kg methotrexate was effectively reduced the arthritis index and significantly decreased inflammatory cell number between 2nd and 6 weeks. CONCLUSION: A high dose of methotrexate may be clinically effective in the short period treatment of rheumatoid arthritis patients.
Subject(s)
Animals , Humans , Male , Rats , Arthritis , Arthritis, Rheumatoid , Cell Count , Collagen , Collagen Type II , Injections, Intraperitoneal , Methotrexate , NorwayABSTRACT
PURPOSE: We had examined the effect of high dose MTX loaded acrylic cement in the treatment of type II collagen induced arthritis. MATERIALS AND METHODS: we used inbreed rat(16weeks - 20weeks) and divided into control group and experimental groups. inbreed rat was induced arthritis by prepared collagen through injection at the tail base. The control group was injected with collagen only. but the experimental groups were inserted intraperitoneally MTX-loaded acryl cement: 25mg, 20mg, 15mg, and 10mg MTX in acrylic cement (0.5gm) respectively. The histopathologic results were investigated to determine the effect of MTX on the 3rd, 7th, 14th, 28th, 42th, and 56th day after inserting high dose MTX loaded acrylic cement which was inserted intraperitoneally 2weeks later of collagenase injection RESULTS: The arthritis index of the MTX-loaded groups had reduced than arthritis index of the control group according to the dosage and In histopathological test, the experimental group injected with 15mg, 20mg, and 25mg of MTX had distinctive effects, compared to the control group, as dosage chronologically. CONCLUSION: The treatment of high-dose MTX loaded acrylic cement might be effective on rheumatoid arthritis managment.