Antihypertensive drug-related genes polymorphisms in hypertensive patients at a certain hospital / 中国热带医学

@#Abstract: Objective　By analyzing the frequency distribution of antihypertensive drug-related genotypes in hypertensionpatients treated in our hospital, so as to provide a clinical basis for individualized treatment of hypertension patients. Methods　A total of 72 hypertensive patients treated in Hainan Hospital of PLA General Hospital from June 2021 to April 2022 were collected. PCR-melting curve method was used to detect CYP2D6*10 (c.100 C>T), CYP2C9*3 (c.1075 A>C), ADRB1 (c.1165 G>C), AGTR1 (c.1166 A>C), ACE (I/D), NPPA (T2238C) and CYP3A5*3 (A6986G), and the relationship between different genotypes and biochemical indexes was analyzed. Results　According to the statistics of the gene and genotype frequency of each point in 72 patients, the gene frequencies of 7 sites all conformed to Hardy Weinberg equilibrium. There were gender differences in ADRB1 genotypes (χ2 = 5.878, P<0.05). There were statistical differences in triglycerides [AA: 1.4 (1.0, 2.0)mmol/L; AC: 2.2 (1.5, 2.5)mmol/L; P=0.038], total cholesterol [AA: 4.0 (3.1, 4.9) mmol/L; AC: 4.8 (4.0, 5.3) mmol/L; P=0.040] and low-density lipoprotein cholesterol [(AA: 2.4 (1.8, 3.3) mmol/L; AC: 3.2 (2.5, 3.5) mmol/L; P=0.035] among patients with different genotypes of AGTR1 locus. The patients with different genotypes of CYP2C9 locus had significant differences in their alanine transferase (ALT) [AA:16.9 (11.4,30.2) mmol/L; AC:10.4 (9.4, 18.2) mmol/L; P=0.040]. Aftergene-directed individualized therapy, different genotypes of CYP3A5 andAGTR1 affected the heart rate [CYP3A5: AA: (79.3±7.0) beats/min; AG： (69.8±6.8) beats/min; GG： (68.8±7.3) beats/min; P=0.010], systolic blood pressure [AGTR1: AA: (131.3±16.7) mmHg; AC: (140.6±11.8) mmHg; P=0.014] and diastolic blood pressure [CYP3A5: AA: (90.0±8.3) mmHg; AG: (78.7±10.8) mmHg; GG: (74.9±10.7) mmHg; P=0.025; AGTR1: AA: (75.3±10.2) mmHg; AC: (86.3±10.6) mmHg; P=0.001] of patients. Conclusions　The related gene loci of antihypertensive drugs are an important basis for guiding the diversification and individualization of clinical medication. Clinicians need to consider the impact of related genes on drug efficacy and adverse reactions when prescribing.

Exploration on the Medication Rules of Traditional Chinese Medicine for Hypertension Treatment through Parallel K-means Optimization Algorithm / 医学信息学杂志

The paper introduces relevant concepts of parallelization,including Mapreduce programming frame and distributed document system of Hadoop,optimizes the traditional K-means algorithm,establishes parallel K-means clustering model,explores the medication rules of Traditional Chinese Medicine (TCM) for hypertension treatment,digs into 8 groups of prescriptions for differentiation treatment of hypertension through TCM,verifies authenticity of the conclusion by combining the theories of TCM.

Design, synthesis and evaluation of antihypertensive activity of novel nitric oxide-releasing N-phenyl-1H-pyrrole derivatives / 中国药学杂志

OBJECTIVE: To synthesize and evaluate of antihypertensive activity of novel nitric oxide-releasing N-phenyl-1H-pyrrole derivatives. METHODS: By connecting key structural elements present in an AT1 receptor antagonist irbesartan with N-phenyl-1H-pyrrole carboxylic acid, a novel AT1 antagonist compound 4 was designed and synthesized, and a series of novel NO-donating derivatives (IN 1-10) were obtained by introducing NO donor. The amount of NO production in vitro of the target compounds were determined by Greiss assay. And the antagonism of Ang II induced vascular contraction assay was used to value the inhibition rate. RESULTS: The antagonism of Ang II induced vascular contraction assay indicated that the novel compound exhibited similar activity as losartan. The NO derivative, compound IN9, found to release the maximum amount of NO during the NO releasing assay, was more potent than the lead compound 4 and positive control losartan. CONCLUSION: These date indicate that the improved activities of these hybrid molecules contribute to the NO donor and the protection ability of NO donor make them promising candidates as antihypertensive agents.

Effect of Polyphenols fromRubus suavissirnuS. Lee on Spontaneous Hypertensive Rats / 世界科学技术-中医药现代化

This article was aimed to study the effect of polyphenols fromRubus suavissirnusS. Lee (RSLP) on spontaneous hypertensive rats (SHR) and to explore its mechanism of anti-hypertensive. The water extraction of RSLP was prepared. And the polyphenols was extracted with macroporous resin. The non-invasive blood pressure analysis system was used to detect the blood pressure. SHR model was selected to study the anti-hypertensive effect. The 16 normal Wistar rats were randomly divided into the control group and the normal RSLP high-dose group (RSLP-NH). The 40 SHR were randomly divided into the model group, Captopril group, RSLP-L group, RSLP-M group and RSLP-H group. SBP, DBP, HR, body weight and organ index were observed after the drug administration for 8 weeks and drug withdrawal for 2 weeks. The contents of SOD, MDA, GSH-Px, NO, NOS and ANP in serum were measured. The results showed that the blood pressure of SHR was significantly higher than that of the control group, which can be used for anti-hypertensive studies. Each RSLP group can obviously reduce the SBP and DBP of SHR (P 0.05). RSLP can elevate GSH-Px, SOD levels and reduce the activity of MDA (P < 0.05). RSLP can reduce NO, NOS and ANP contents in serum (P < 0.05). It was concluded that RSLP can significantly reduce the SBP and DBP of SHR, but it had no significant effect on HR. It can increase the activity of GSH-Px, SOD, NO, NOS levels, and reduce the contents of MDA, ANP in serum. It had certain inhibitory effect on the left ventricular hypertrophy.

The analysis of current situation of hypertension control and its influencing factors among geriatric patients in Hangzhou, Jiaxing and Shaoxing in Zhejiang province / 中华老年医学杂志

Objective From October 2010 to explore the current situation of hypertension control and its influencing factors among geriatric patients in Hangzhou,Jiaxing and Shaoxing in Zhejiang province.Methods To April 2011,10 644 geriatric patients aged 60 years and over were included,and related questionnaire survey,physical examination and laboratory examination were carried out.The situation of hypertension control and influencing factors were analyzed.Results The control rate of hypertension was 38.6％,and its standardized rate was 27.6％.It showed that female,inhabited city,high level of education degree and regular exercise were positively correlated with hypertension control,while age,smoking,high salt diet and abdominal obesity were negatively correlated with hypertension control,and standardized treatment had a close relationship with hypertension control.Conclusions The situations of hypertensive control among geriatric patients in Hangzhou,Jiaxing,Shaoxing in Zhejiang province are still unsatisfactory.Strengthening standardization management measures for improving the control rate of hypertension is needed and has actual value.

Hemodynamic effects of aqueous extract of Artemisia campestris in adult men.

Arterial hypertension is a worldwide health problem with multiple origins. Its management remains difficult, particularly because of the pathology dependence on human lifestyle. Several ethno pharmaceutical are used to treat hypertension. Herein we investigated, for the first time, the effect of the water-boiled extract of Artemisia campestris on Human hemodynamic system. To do so, the blood pressure parameters of two groups of adult volunteers (smokers and non-smokers) were recorded before and each 15 minutes after drinking 20 ml of the boiled extract (20g of dried leaves / 1 L of water). The diastolic pressure and heart rate significantly diminished following the treatment, in both smoker and non-smoker men. In exception to a punctual reduction at 45 minutes in non- smokers, the systolic pressure did not significantly change. The classification of subjects in accordance to their hypertension statute at each time-point of recording, showed an important reduction of the stage 2 in smoking men, following the consumption of the extract. It is concluded that the water-boiled extract of Artemisia campestris could tip over arterial hypertension, especially in smokers. It is suggested that the mechanism of such effect involves epinephrine synthesis or binding to its receptor inhibition.

Use of anti-hypertension drugs in childhood hypertensive crisis / 中国小儿急救医学

Childhood hypertensive crisis is a relatively rare emergency event,and is associated with a high risk of morbidity and mortality.Children in this situation need rapid,safe and effective treatment to release clinical symptoms,relieve injuries of target organs,improve quality of life,and reduce mortality.Many kinds of anti-hypertension drugs have been used in children for decades,but most data were extrapolated from studies in adult.There is limited number of randomized controlled trials in children.Therefore it is still difficult to choose drugs for pediatric hypertensive crisis based on evidence-based medicine.This paper sought to summarize the experience of medical management of childhood hypertensive crisis in children.

Non-anti-hyperlipemic effects of Monascus-fermented rice and its mechanism: Recent advances / 中华临床营养杂志

Monascus-fermented rice is a natural food and traditional Chinese medicine. Besides its wellknown effectiveness in lowering blood lipids, Monascus-fermented rice is also beneficial for decreasing blood pressure, preventing osteoporosis, and lowering blood sugar. This article reviews recent advances in its non-anti-hyperlipemic effects and their potential mechanisms.

Effect of Anti-hypertensive Therapy on the Characteristics of Platelet in Primary Hypertension Patients / 中华高血压杂志

Objective To study the effect of anti-hypertensive therapy on the characteristics of platelet in essential hypertension(EH) patients. Methods Thirty-two healthy people(CT) and 37 patients with primary hypertension (gradeⅠ) were recruited . Blood pressure and 4 platelet parameters: platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW) and platelet hematocrit (PCT) were determined by whole blood automatic analysis apparatus. Results The 4 platelet parameters were increased in hypertension patients compared with healthy people and decreased after anti-hypertensive therapy[PLT:(before 201.8?72.9 vs after 187.1?68.6 )10~ 9 L+{-1}; MPV:(before 10.3?1.9 vs after 10.0?1.9)fL, PDW:(before 15.5?1.1 vs after 15.2?1.0)fL; PCT:(before 0.198%?0.047% vs after 0.187%?0.036%)](P

Comparação dos perfis dos pacientes hipertensos atendidos em pronto-socorro e em tratamento ambulatorial / Perfil de los pacientes hipertensos atendidos en un servicio de emergencia: comparación con hipertensos en tratamiento ambulatorio / Comparison of the profile of hypertensive patients seen in emergency unit with those receiving outpatient clinic treatment

O estudo comparou 100 hipertensos atendidos no Pronto-socorro com 100 pacientes do Ambulatório. Os hipertensos do Pronto-Socorro foram diferentes (p < 0,05) em relação a: maior pressão arterial; menor renda salarial; maior consumo de bebida alcoólica; não pertenciam à comunidade do hospital; descobriram ser hipertensos por sentirem-se mal; mediram menos a pressão; e deixaram de tomar mais medicamentos. A análise multivariada revelou diferenças significativas entre os dois grupos quanto à renda, ao local onde é medida a pressão e não tomar os medicamentos. Concluiu-se que características desfavoráveis podem contribuir para não realizar o tratamento anti-hipertensivo, levando a atendimentos em unidades de emergência.

This study compares 100 hypertensive patients in emergency units with those who were seen in outpatient clinics. Hypertensive patients seen at the emergency unit showed to be different (p < 0.05) from those seen at the outpatient clinic regarding: higher blood pressure; lower income; not belonging to the hospital community; greater alcohol intake; became aware of their hypertension because felt bad; measured blood pressure less often; did not take the medicine more often. In conclusion, unfavorable characteristics can contribute to hypertensive patients who do not follow anti-hypertension treatment adequately, leading to care in emergency units.

Este estudio comparó 100 hipertensos atendidos en un servicio de emergencia, con 100 pacientes de consulta externa. Los hipertensos de emergencia fueron diferentes (p<0,05) en relación a: presión arterial más elevada; menor renta salarial; no pertenecían a la comunidad del hospital; mayor consumo de bebidas alcohólicas; descubrieron ser hipertensos por sentirse mal; se midieron menos la presión y dejaron de tomar los medicamentos. El análisis multivariado muestra que la renta, el local donde se mide la presión y dejar de tomar los medicamentos marcaron diferencias entre los dos grupos. Se concluyó que estas características desfavorables pueden contribuir al abandono del tratamiento antihipertensivo llevando a los pacientes a ser atendidos en unidades de emergencia.