MicroRNA-490-5p inhibits invasion and migration of human intrahepatic cholangiocarcinoma RBE cells / 肿瘤

Objective: To investigate the expression of microRNA-490-5p (miR-490-5p) in human intrahepatic cholangiocarcinoma tissues, and to study its impacts oninvasion and migration of intrahepatic cholangiocarcinoma RBE cells and the underlyingmolecular mechanism.Methods: The expression level of miR-490-5p in 5 pairs of intrahepatic cholangiocarcinomatissues and the adjacent normal tissues was detected by real-time fluorescent quantitativePCR. Intrahepatic cholangiocarcinoma RBE cells were transfected with miR-490-5p-mimicsand the negative control by LipofectAMINE 2000, respectively. Then the cell migration andinvasion abilities were explored by scratch wound healing assay and Transwell chamberassay, the proliferation of RBE cells were measured by CCK-8 assay, the colony formationability was detected by colony formation assay, the apoptosis rate was detected by FCMmethod. The target genes of miR-490-5p were predicted by online miRNA-targeted geneprediction software, and validated by Western blotting.Results: The expression level of miR-490-5p was significantly down-regulated in humanintrahepatic cholangiocarcinoma tissue as compared with the adjacent normal tissues (P 0.05), but the scratch healing, migrationand invasion abilities of RBE cells were significantly decreased (all P < 0.05). TargetScanwebsite predicted that the potential target gene of miR-490-5p was FBJ osteosarcomaoncogene (c -Fos ), and Western blotting verified that the expression of c-Fos was significantlydown-regulated in RBE cells transfected with miR-490-5p-mimics.Conclusion: MiR-490-5p is lowly expressed in human intrahepatic cholangiocarcinoma, andmay regulate the migration and invasion of intrahepatic cholangiocarcinoma RBE cells bydown-regulating the expression of c -Fos gene.

The expression of c-fos gene in retina of flickering light-induced and form deprivation myopia / 中华实验眼科杂志

Background Flickering light is different from the normal light environment.Animal experiment proved that flickering light can induce myopia.But its mechanism remains unclear.Objective This study was to investigate the expression of c-fos gene in retina of myopic C57BL/6J mice induced by flickering light and monocular form deprivation.Methods Ninety clean C57BL/6J mice aged 28-day-old with the similar refraction in both eyes were randomly assigned to five groups.Fifteen mice in the control group were exposed to continuous white light environment.The white flickering light with the frequency of 10,5,2 Hz were used to irradiate the mice respectively in high frequency flickering group (15 mice),moderate frequency flickering group (15 mice) and low frequency flickering group (15 mice),respectively.The right eyes of other 30 mice were monocularly occluded with a semitransparent hemispherical thin plastic shell to establish the form deprivation models and then were exposed to white light environment.The diopter and ocular axial length were measured by murine-specific eccentric infrared photorefraction and A-scan ultrasonography before experiment and two weeks after the treatments.At the end of experiment,the mice were sacrificed by neck dislocation.Mice eyes were enucleated and retinal samples were prepared for the detect of c-fos protein and its mRNA by immunohistochemistry,Western blot and reverse transcription polymerase chain reaction (RT-PCR),respectively.Results Immunohistochemistry showed that the expressing rate ofc-fos protein in retina was (68.000±10.368)％,(51.000±6.519)％,(46.000±6.519)％,(31.000±7.416)％ and (25.000 ± 7.071)％ in the control group,high frequency flickering group,moderate frequency flickering group,low frequency flickering group and form deprivation group respectively 2 weeks after experiment.The expression rates of c-fos protein in retina in different frequencies of flickering light groups and form deprivation group were significantly lower than that in the control group (t =3.104,4.017,6.490,7.661,all P＜0.05),with the lowest rate in the form deprivation group (P＜0.05).The expression of c-fos detected by Western blot assay exhibited that the relative values of c-fos protein in retina (c-fos/GAPDH) was 0.804±0.050,0.687±0.047,0.667±0.036,0.558±0.036 and 0.532 ±0.056,respectively in the control group,high frequency flickering group,moderate frequency flickering group,low frequency flickering group and form deprivation group,illustrating significantly lowing in different frequencies of flickering light groups and form deprivation group compared with control group (t =2.961,3.184,6.971,6.276,all P＜0.05),whereas the c-fos in the low frequency group and form deprivation group,c-fos protein was less expressed in comparison with the higher frequency flicking group (P＜0.05).The expression level of c-fos mRNA (c-fos mRNA/GAPDH mRNA) in retina was 0.820±0.056,0.663±0.061,0.627±0.034,0.521±0.041 and 0.474 ±0.045 in the control group,high frequency flickering group,moderate frequency flickering group,low frequency flickering group and form deprivation group,respectively.These results demonstrated a significant decline in the expression of c-fos mRNA in different frequencies of flickering group and form deprivation group compared with the control group(t=3.262,5.070,7.173,8.305,all P＜0.05),and the inhibition ability of low frequency of flickering group and form deprivation group was much stronger.Conclusions The c-fos gene level in the retina has a negative relationship with the severity of myopia induced by flickering light and form deprivation.

Advance in Research of Expression of c-fos Gene in Learning and Memory and Exercise System (review) / 中国康复理论与实践

@#c-fos, as one of immediate-early gene, affected the learning and memory through influencing the transcription and translation, thereby arousing the extensive recognition and become one of the objective guideline. The author reviewed the newest literature in relation to c-fos gene in learning and memory and exercise in order to provide reference for the application in the exercise physiology.

The effects of superoxide dismutase on the expression of c-fos gene in the chronic post-ischemic pain model rats / 대한마취과학회지

BACKGROUND: Chronic post-ischemia pain (CPIP) model is reported to represent the complex regional pain syndrome type I. The administration of non-specific free radical scavengers reduced mechanical allodynia, but it is not evident which type of free radical is responsible for the development of CPIP. This study was investigated to elucidate the role of superoxide on the development of CPIP and the relationship with the expression of c-fos gene. METHODS: Male Sprague-Dawley rats weighing 290-310 g were housed in one cage with food and water ad libitum. CPIP model was made by placing a tourniquet on the left hindpaw of rats. The tourniquet maintained for 3 hours, then released to allow reperfusion. Thirty minutes before reperfusion, superoxide dismutase (SOD) or normal saline (control group) was injected. Mechanical allodynia and cold allodynia were measured at 1, 3, 5, 7, 14 and 28 days after reperfusion and compared. Also, spinal cord was harvested and the expression of c-fos gene was measured through the real time reverse transcription polymerase chain reaction. RESULTS: Superoxide dismutase reduced mechanical allodynia (1, 3, 5 and 14 day) and cold allodynia (1, 3 and 7 day) compared with control rats in left hindpaw. Expression of c-fos was significantly reduced in the SOD rats at the day 14 and 28 compare to the control rats. CONCLUSIONS: The administration of superoxide dismutase suppressed the allodynia and c-fos gene expression of CPIP model rats and it may be suggested that the superoxide has an important role in the development of CPIP.

Effects of Genistein on The Expression of Estrogen Receptor ? and c-fos Gene in Aorta in Ovariectomized Rat / 中华高血压杂志

Objective To study the effects of genistein(GST) on the expression of estrogen receptor ?(ER?) and the expression of c-fos gene in the aorta of ovariectomized rats.Methods Forty female Wistar rats were randomly divided into four groups:sham-operated(control),ovariectomized(OVX),ovariectomized with 17?-E2 replacement(OVX+E2),ovariectomized with genistein replacement(OVX+GST) group.After 8 weeks' replacement therapy,the rats were sacrificed and the expression of ER? and c-fos in the aorta was studied by immunohistochemistry.Results Ovariectomy significantly decreased ER ? expression and increased c-fos expression in aorta;while replacement therapy,GST and E2,attenuated the effect of ovariectomy manifested by increasing ER expression and decreasing c-fos expression in aorta.Conclusion GST may modulate the expression of ER? in the arteries of ovariectomized rats and play a beneficial effects on cardiovascular system which was associated with decreases in expression of c-fos gene in aorta.

Effect of Total Flavonoids of Hedysarum Polybotry on Inducing Differentiation of Leukemic Cells / 中国中医药信息杂志

Objective:To investigate the effect and mechanism of total flavonoids of Hedysarum polybotry on induction of differentiation in human leukemia HL-60 cells. Method After the treatment of HL-60 cells with total flavonoids of Hedysarum polybotry, the cell differentiation was detected with NBT reduction method. Cell cycle, CD11b and C-fos were analysed by the flow cytometry. Result The positive rate of NBT reduction and the expression of CD11b were significantly increased. Similar, the expression of C-fos gene was upregulated. The growth of HL-60 cells was arrested at G0/G1 and G2/M phase. Conclusion Total flavonoids of Hedysarum polybotry could induce differentiation of HL-60 cells. Its molecular mechanism might be related to the modulation of gene expressions associated with the proliferation and differentiation, which leads to the inhibition of DNA synthesis.

Role of the Peripheral Vestibular System on Neuroplasticity Induced by Hypergravity Stimulation

BACKGROUND AND OBJECTIVES: Altered environmental gravity, including both hypo- and hypergravity, may result in space adaptation syndrome. To explore the characteristics of this adaptive plasticity, the expression of immediate early gene c-fos mRNA in the vestibular system following an exposure to hypergravity stimulus was determined in rats. MATERIALS AND METHOD: The animals were subjected to 2 G force (two-fold earth's gravity) stimulus for 3 hours, and were examined at post-stimulus hours 0, 2, 6, 12, and 24. Real time reverse transcription-polymerase chain reaction (RT-PCR) was adopted to analyze temporal changes in the expression of c-fos mRNA. RESULTS: The hypergravity stimulation produced the expression of c-fos mRNA in the vestibular ganglion, medial vestibular nucleus, inferior vestibular nucleus, hippocampus, vestibulocerebellum, and vestibular cortex. The peak expression occurred at hour 6 in the animals hypergravity-stimulated for 3 hours. Bilateral labyrinthectomy significantly attenuated the degree of up-regulation in c-fos mRNA expression. MK-801, an NMDA receptor antagonist, also significantly attenuated the degree of up-regulation in c-fos mRNA expression. CONCLUSION: These results indicate that the adaptive neuroplasticity in response to an altered gravity occurs in the vestibular-related organs in the central nervous system, in which peripheral vestibular receptors and NMDA receptors play an important role.

The inhibition of genistein on proliferation and transcription of c-fos mRNA in human umbilical vascular smooth muscle cells induced by MCP-1 / 中国药理学通报

Aim To study the inhibition of genistein on proliferation and transcription of c-fos mRNA in human umbilical vascular smooth muscle cells(hUVSMC) induced by monocyte chemotactic protein-1(MCP-1). Methods Growth-arrested hUVSMC were stimulated with MCP-1(10 ?g?L-1) prior to co-treatment with different concentrations of genistein (10,30,90 ?mol?L-1). The response of hUVMSC to these treatments was observed in comparison with that of control group. The proliferation of hUVMSC was evaluated by cell counting. The expression of c-fos mRNA was detected by RT-PCR. Results Low concentration of genistein(10 ?mol?L-1) inhibited the proliferation of hUVSMC and high concentration of genistein(30,90 ?mol?L-1) inhibited the expression of c-fos in hUVSMC induced by MCP-1. Conclusions Genistein could suppress the proliferation of hUVSMC induced by of MCP-1. Its mechanisms may involve the down-regulation of c-fos mRNA expression.

Inhibition effects of basic fibroblast growth factor on c-fos gene expression after tractive spinal cord injury in rats / 第三军医大学学报

Objective To observe the effects of basic fibroblast growth factor on c-fos gene expression in spinal cord neurons of rats after tractive spinal cord injury. Methods The animal model of SD rats with tractive spinal cord injury was established through tracting T_ 13-L_2 spine longitudinally with a special spinal tractor that was put on parapophysis of T_ 12-L_3 vertebrae after exposing T_ 13-L_2 spinal cord via dual laminectomy, meanwhile spinal cord function was monitored by cortical somatosensory evoked potential (CSEP). Forty model rats were randomly divided into 2 groups, the bFGF treatment group (n=20) with bFGF injection into the subarachnoid cavity and the control group (n=20) with saline injection. Five SD rats without any treatment served as normal control. The c-fos gene expression was detected with immunohistochemistry and analyzed quantitatively with image system of computer. Results The c-fos gene expression in tractive spinal cord injury was higher than that in normal control group, especially increased markedly in damaged neurons. The peak value of c-fos gene appeared in 2 h after the injury, but c-fos level in the bFGF treatment group was lower evidently than that in the control group at the different sacrificed time (P

The effect of shortly inhaled isoflurane or enflurane on c-fos gene expression of limbic system in rat / 中国药理学通报

AIMTo investigate the effect of shor tly inhaled isoflurane or isoflurane on c-fos gene expression of limbic system in rat. METHODS18 SD male rats weighing 200～250 g were randomi zed into three groups, control group, isoflurane group, enflurane group.The anim als in enflurane group or isoflurane group breathed 2% enflurane or 2% isofluran e till righing reflex nearly disappear.The animals in control group underwent th e same experimental steps except inhalation of anesthesia.The effect of shortly inhaled isoflurane or enflurane on c-fos gene in limbic system was observed wit h Fos immunohistochemical staining technique. RESULTNumber of FL I positive neurons of the 10 nucleus was increased significantly in limbic syst em by isoflurane, but enflurane only influenced FLI positive neurons expression of 8 out of the 10 nucleus. CONCLUSIONNuclei with significant ch ange of c-fos expression might be related to isoflurane or enflurane anesthes ia induction.