ABSTRACT
Objective To investigate the cytotoxic constituents from the sponge Hyrtios erectus.Methods The constituents were isolated and purified by column chromatography,and their structures were identified by physicochemical data,spectral data,and comparison with data of references.The cytotoxic activity of all the compounds was determined by MTT mothod.Results Five compounds were isolated from the lipid extract of Hyrtios erectus,and their structures were elucidated as Hyrtiosal(Ⅰ),12?-Hydroxy-16-scalaren-24,25-olide(Ⅱ),5-Hydroxy-1H-indole-3-ethanol(Ⅲ),cholesterol(Ⅳ),and dibutylphthalate(Ⅴ) respectively.Conclusion Compound were isolated from Hyrtios erectus for the first time, and compound Ⅰand Ⅱ showed strong cytotoxic activity.
ABSTRACT
Objective To investigate the growth inhibitory effect of the extracts from sixteen species of mangrove plants in Hainan on HeLa cells. Methods Sixteen species of mangrove plants were collected in Wenchang county,Hainan province,and were extracted with ethanol.The ethanol extracts were dissolved in water,partitioned by ethyl acetate,and ethyl acetate and water extract were obtained.Growth inhibitory rates of the extracts on HeLa cells were determined by MTT assay.Results The ethyl acetate extracts from six species of mangrove plants showed growth inhibitory activity on HeLa cells,and the extracts from Acrostichum aureurm and Heritiera littoralis showed strong activity,the IC_(50) values of which were 6.3,9.5 ?g mL~(-1),respectively.Conclusion The ethyl acetate extracts from six species of mangrove plants showed growth inhibitory effects on HeLa cells,whereas the water extracts did not.