ABSTRACT
In this approach, a new voltammetric method for determination of norfloxacin was proposed with high sensitivity and wider detection linear range. The used voltammetric sensor was fabricated simply by coating a layer of graphene oxide (GO) and Nafion composited film on glassy carbon electrode. The advantage of proposed method was sensitive electrochemical response for norfloxacin, which was attributed to the excellent electrical conductivity of GO and the accumulating function of Nafion under optimum experimental conditions, the present method revealed a good linear response for determination of norfloxacin in the range of 1×10-8mol/L-7×10-6 mol/L with a detection limit of 5×10-9 mol/L. The proposed method was successfully applied in the determination of norfloxacin in capsules with satisfactory results...
Propos-se, por essa abordagem, novo método voltamétrico, com alta sensibilidade e faixa linear de detecção mais ampla, para a determinação de norfloxacino. O sensor voltamétrico utilizado foi fabricado simplismente por cobertura de camada de óxido de grafeno (GO) e filme de Nafion em eletrodo de cabrono vítreo. A vantagem do método proposto foi a resposta eletroquímica sensível para o norfloxacino, atribuída à condutividade elétrica excelente do GO e à função acumulada do Nafion. Sob condições experimentais ótimas, o presente método revelou boa resposta linear para a determinação do norfloxacino na faixa de limite de detecção de 1×10-8mol/L-7×10-6 mol/L. O método proposto foi aplicado com sucesso na determinação de norfloxacino em cápsulas, com resultados satisfatórios...
Subject(s)
Humans , Norfloxacin/analysis , Chemistry, Pharmaceutical/methods , Electrochemical Techniques/instrumentation , Electrochemical Techniques/methodsABSTRACT
An accurate, simple, rapid, and sensitive spectrofluorimetric method was developed for the determination of two fluoroquinolone anti-bacterial Ciprofloxacin [Cip] and Norfloxacin [Nor]. This spectrofluorimetric method is based on the direct measuring of [Cip] and [Nor] in aqueous solutions through using a Britton buffer at pH 7.0 and pH 3.0 For Cip, Nor respectively. The wavelength of both excitation and emission is: Ex=330 nm, Em=422 nm for Cip and Ex=358 nm, Em=447 nm For Nor. Calibration graphs for Cip and Nor are linear at the range 0.0331-0.464 micro g mL[-1], 0.1227-1.5326 micro g mL[-1], The detection limit is 0.0331 and 0.1227 micro g mL[-1] with a relative standard deviation not exceed 5.28% and 5.04%. For Cip and Nor respectively. The proposed method has been successfully applied to be determine either in pure form or in the drugs in their tablets, vial and eye drops formulations, and the method enables for the determination of Cip - Nor in pharmaceuticals without interference from associated substance. The suggested method has the advantage of being applicable for the determination of two drugs without prior extraction nor forming a complex. The method developed by us specifically increased sensitivity about 12 times for the determination of Cip, about 2.5 times for the determination of Nor. The new developed method is simple and rapid with high accuracy and sensitivity, and it is useful for determination Cip and Nor in dosage forms
Subject(s)
Norfloxacin/analysis , Spectrometry, Fluorescence , FluoroquinolonesABSTRACT
Norfloxacino-quimioterápico antibacteriano sintético ativo contra bactérias Gram-negativas - foi doseado quantitativamente por volumetria em meio näo-aquoso com ácido perclórico. Verificou-se que o indicador vermelho de quinaldina oferece melhores resultados juntamente com a perfeita visualizaçäo do ponto de equivalência