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1.
Vitae (Medellín) ; 30(3): 1-12, 2022-09-22. Ilustraciones
Article in English | LILACS, COLNAL | ID: biblio-1538064

ABSTRACT

Background: Many native fermented foods in Latin America are made by indigenous communities through processes that preserve their traditions. Among these products is champús, a fermented drink or dessert native to Colombia, Ecuador, and Peru. It has a characteristic flavor between sweet and sour with a low alcohol content, is made from cereals such as corn, wheat, or a mixture of these, and can include fruits and spices. Objectives: This research evaluated the microbiological, physicochemical, and sensory quality characteristics of champús to revalue the consumption of traditional foods. Methods: For this purpose, the microbiological safety criteria, fermenting microorganisms, and physicochemical and sensory parameters were evaluated for two batches of champús prepared by five producers and taking samples at different stages. Results: It was found that the average temperature and relative humidity for processing were between 16.5 ± 2.56 °C and 61.6 ± 9.14 %, respectively, and the products exceeded microbiological criteria such as total coliforms and some microbiological criteria for Escherichia coli and Staphylococcus aureus. There was also a high prevalence of lactic acid bacteria and yeasts throughout the process, with the occurrence of the genera Weissella, Leuconostoc, and Lactobacillus. The presence of organic acids (lactic, acetic, and propionic) and ethanol was evidenced. Champús seems to be a source of calcium, phosphorus, and free amino acids. Conclusions: This information provides a basis to propose improvement plans for small producers and, at the same time, to conduct more detailed studies on the native microbiota of champús


Antecedentes: Muchos de los alimentos nativos fermentados de América Latina son elaborados por las comunidades indígenas mediante procesos que preservan sus tradiciones. Entre estos productos se encuentra el "champús", una bebida o postre fermentado originario de Colombia, Ecuador y Perú. Presenta un sabor característico entre dulce y ácido con un bajo contenido de alcohol, está elaborado a base de cereales como maíz, trigo o la mezcla de estos, y puede llevar entre sus ingredientes frutas y especias. Objetivo: Esta investigación evaluó las características de calidad microbiológica, fisicoquímica y sensorial del champús para revalorizar el consumo de los alimentos tradicionales. Métodos: Se evaluaron criterios de seguridad microbiológica, microorganismos fermentadores, parámetros fisicoquímicos y sensoriales de dos lotes de champús elaborados por 5 productores, tomando muestras en diferentes etapas de proceso. Resultados: Como resultados, se encontró que la temperatura y la humedad relativa promedio de elaboración estuvieron entre 16,5 ± 2,56 °C y 61,6 ± 9,14 % y que los productos superaron los criterios microbiológicos como coliformes totales y algunos criterios microbiológicos como Escherichia coli y Staphylococcus aureus. También hubo una alta prevalencia de bacterias lácticas y levaduras a lo largo del proceso, identificándose los géneros Weissella, Leuconostoc y Lactobacillus. Se evidenció la presencia de ácidos orgánicos (láctico, acético y propiónico) y de etanol. El champús parece ser una fuente de calcio, fósforo y aminoácidos libres. Conclusión: Esta información sirve de base para proponer planes de mejora para los pequeños productores y al mismo tiempo para realizar estudios más detallados sobre la microbiota nativa del champús.


Subject(s)
Humans , Fermented Foods , Microbiological Techniques , Fermented Beverages , Latin America
2.
Acta Medica Philippina ; : 6-17, 2022.
Article in English | WPRIM | ID: wpr-988662

ABSTRACT

Background@#Tapuy is an indigenous fermented rice wine produced in the Northern areas of the Philippines. Fermented foods and drinks have gained interest due to their associated health benefits, attributable to probiotic bacteria in the food items. However, pathogenic bacteria may also be present in fermented food and pose health risks, signifying a need for standardization. Currently, there is limited knowledge on the bacterial content of tapuy. @*Objectives@#This study aimed to characterize the bacterial diversity and community structure of culturable bacteria in traditionally fermented tapuy samples, to perform standardization of tapuy fermentation, and to compare the bacterial diversity and community structure of culturable bacteria in laboratory fermented tapuy with that of the traditional samples. @*Methods@#Tapuy samples were obtained from four municipalities in Benguet, Philippines. Laboratory fermentation of tapuy was performed simultaneously with the fermentation in the sampling site using a standardized protocol. Samples were plated on de Man, Rogosa, and Sharpe (MRS) agar and NA, and the colonies were harvested for DNA extraction. DNA samples were sent for 16S rDNA metagenomic sequencing. Results. Metagenomic analysis revealed the presence of many bacterial species that were previously unreported in tapuy. Traditional tapuy samples were composed primarily of members of the genera Bacillus and Lactobacillus in varying proportions. Potential probiotic bacteria were abundant in Kapangan (97.42%) and Sablan (99.89%) field samples. B. wiedmannii was present in all samples and was identified as a harmful species. Laboratory fermentation increased the abundance of potential probiotic bacteria in Itogon and La Trinidad samples (differences of 75.36% and 78.36%, respectively). It decreased the quantity of B. wiedmannii in La Trinidad (a difference of 97.1%). Laboratory fermented samples generally exhibited higher bacterial diversity and species richness compared to field samples. @*Conclusions@#Traditionally fermented tapuy samples contained a significant proportion of potential probiotic bacteria belonging to the genera Bacillus and Lactobacillus. Laboratory fermented samples were found to have higher bacterial diversity and richness compared to field samples. The significant presence of potential probiotic bacteria suggests that tapuy is a good candidate for development into functional food and a good source of likely probiotic species that could be explored for health applications. The presence of harmful bacteria suggests the need for possible standardization of fermentation practices.


Subject(s)
Food Microbiology , Fermented Foods , Wine
3.
São Paulo; s.n; s.n; 2022. 61 p. tab, graf, ilus.
Thesis in Portuguese | LILACS | ID: biblio-1415198

ABSTRACT

Os casos de transtorno de ansiedade têm apresentado crescimento considerável desde o início do século XX, onde a terapia medicamentosa oferecida, geralmente apresenta efeito sedativo, portanto, a busca por tratamentos adjuvantes para tratar quadros de ansiedade se fazem necessários. Estudos indicam que a modulação da microbiota intestinal pode estar relacionada à regulação neural dos indivíduos através de diversas vias, incluindo a aplicação de cepas probióticas e consumo de alimentos fermentados tradicionais como iogurte e kombucha, colaborando para a melhoria da qualidade de vida destes pacientes. Este projeto teve como objetivo buscar os metabólitos e neurotransmissores presentes no kombucha a fim de verificar seu potencial psicobióticos e comparar as aplicações e metabólitos produzidos por cepas probióticas existentes no mercado e em alimentos fermentados tradicionais que atuem no eixo intestino-cérebro. Foram realizadas pesquisas em bases de dados online, como Pubmed, Web of Science, Scielo, Scopus e Google Scholar no período entre 2002 e 2022 relacionados aos possíveis efeitos dos probióticos em condições de ansiedade, bem como como os mecanismos que envolvem o eixo cérebro-intestino, seja por meio de testes em humanos e em modelos animais. As espécies mais testadas quanto ao seu potencial probiótico e ação nos transtornos de ansiedade encontradas foram Lactobacillus paracasei, L. casei, L. rhamnosus, Bifidobacterium infanti e B. longum. Cada gênero demonstra um grau diferente na redução da ansiedade dos indivíduos. Os alimentos potencialmente probióticos, incluindo alimentos fermentados tradicionais, além de atuar como complemento à terapia em quadros de ansiedade, tem relevância no setor socioeconômico


Anxiety disorder cases have shown considerable growth since the beginning of the 20th century, where the drug therapy offered usually has a sedative effect. Therefore, the search for adjuvant treatments to treat anxiety disorders is necessary. Studies indicate that the modulation of the intestinal microbiota may be related to the neural regulation of individuals in several ways, including the application of probiotic strains and consumption of traditional fermented foods such as yogurt and kombucha, contributing to the improvement of the quality of life of these patients. This project aimed to identify and compare the psychobiotic effect in the gut-brain axis of the metabolites and neurotransmitters produced by kombucha and commercial probiotic strains. The research was carried out in online databases, such as Pubmed, Web of Science, Scielo, Scopus, and Google Scholar in the period between 2002 and 2022 related to the possible effects of probiotics in anxiety conditions as the mechanisms that involve the brain-gut axis either through tests in humans or animal models. The species most tested for their probiotic potential and action on anxiety disorders were Lactobacillus paracasei, L. casei, L. rhamnosus, Bifidobacterium infanti, and B. longum. Each genus demonstrates a different degree of reducing individuals' anxiety. Potentially probiotic foods, including traditional fermented foods, acting as a complement to therapy in cases of anxiety, have relevance in the socioeconomic sector


Subject(s)
Phobic Disorders/pathology , Kombucha Tea/analysis , Kombucha Tea/adverse effects , Serotonin/analogs & derivatives , Microbiota , Fermented Foods/adverse effects , Brain-Gut Axis
4.
São Paulo; s.n; s.n; 2022. 94 p. tab, graf, ilus.
Thesis in Portuguese | LILACS | ID: biblio-1396412

ABSTRACT

Um dos maiores desafios no desenvolvimento de produtos probióticos é entender como os microrganismos interagem entre si e com o hospedeiro. Quando falamos em alimentos fermentados tradicionais, este obstáculo aumenta porque a matriz alimentar já possui um microbioma intrínseco. No entanto, também é conhecido que muitos microrganismos podem interagir e cooperar para sobreviver quando condições de estresse são encontradas. Assim, o objetivo deste trabalho foi isolar leveduras de quatro diferentes kombuchas em distintos momentos fermentativos e verificar a influência que leveduras isoladas de kombucha têm na manutenção da viabilidade da bactéria probiótica Bifidobacterium animalis subsp. lactis HN019 em condições de aerobiose. Meyerozyma guilliermondii, Candida albicans, Rhodotorula mucilaginosa e Pichia membranifaciens foram leveduras encontradas nas kombuchas, das quais as duas últimas favoreceram a manutenção da alta viabilidade de HN019 em cocultura por 14 dias. Observou-se a viabilidade da bactéria acima de 9 log ao longo de todo o experimento, o que não foi observado em monocultura. Ademais, utilizou-se de análise de autoagregação, hidrofobicidade, atividade enzimática de proteases e fosfolipases das leveuras para analisar seu potencial patogênico. Observou-se que R. mucilaginosa demonstrou características semelhantes à Saccharomyces cerevisiae subsp. boulardii, e sua interação benéfica com HN019 reforça a possibilidade de que esta levedura seja uma chave para a inserção da bactéria em uma kombucha probiótica. Análises metabólicas foram realizadas e encontrou-se uma vasta diversidade de dipeptídeos, principalmente os compostos de prolina, durante a cocultura da bactéria com as leveduras. Tais dipeptídeos apresentam importantes mecanismos de ação no controle biológico e quorum sensing de bactérias e leveduras, e supostamente regulam a manutenção das relações mutualísticas entre ambos microrganismo


One of the biggest challenges in the development of probiotic products is to understand how microorganisms interact with each other and with the host. When we talk about traditional fermented foods, this obstacle increases because the food matrix already has an intrinsic microbiome. However, it is also known that many microorganisms can interact and cooperate to survive when stressful situations are encountered. Thus, the objective of this work was to isolate yeasts from four different kombuchas at different fermentation times and to verify the influence that yeasts isolated from kombucha have on maintaining the viability of the probiotic bacterium Bifidobacterium animalis subsp. lactis HN019 under aerobic conditions. Meyerozyma guilliermondii, Candida albicans, Rhodotorula mucilaginosa and Pichia membranifaciens were yeasts found in kombuchas, of which the last two favored the maintenance of HN019 high viability in co-culture for 14 days. Bacteria viability above 9 log was observed throughout the experiment, which was not observed in monoculture. In addition, analysis of autoaggregation, hydrophobicity, enzyme activity of proteases and phospholipases of yeasts was used to analyze their pathogenic potential. It was observed that R. mucilaginosa demonstrated characteristics similar to Saccharomyces cerevisiae subsp. boulardii, and its beneficial interaction with HN019 reinforces the possibility that this yeast is a key to the insertion of the bacterium in a probiotic kombucha. Metabolic analysis were performed and a wide diversity of dipeptides, mainly proline-based, was found during the co-culture of the bacteria with the yeasts. Such dipeptides have important mechanisms of action in the biological control and quorum sensing of bacteria and yeast, and supposedly regulate the maintenance of mutualistic relationships between both microorganism


Subject(s)
Yeasts/classification , Kombucha Tea/analysis , Fermented Foods/analysis , Rhodotorula/classification , Coculture Techniques/methods , Probiotics , Dipeptides/agonists , Microbiota , Bifidobacterium animalis/pathogenicity
5.
Arch. argent. pediatr ; 119(1): 56-61, feb. 2021.
Article in English, Spanish | LILACS, BINACIS | ID: biblio-1147089

ABSTRACT

La leche materna provee microorganismos que colonizan el intestino y programan el sistema inmunológico para desarrollar tolerancia oral. Entre los 6 meses de lactancia materna exclusiva y los 2 años de lactancia prolongada recomendada, la alimentación complementaria conlleva una reducción progresiva en el ingreso de microorganismos vivos al ecosistema intestinal. Esto se debe a que los alimentos en general -a diferencia de la leche materna- o se encuentran desprovistos de microorganismos o, si los poseen, suelen inactivarse durante la cocción. Los alimentos fermentados y los probióticos podrían constituir una estrategia nutricional valiosa, dado que garantizarían la provisión de microorganismos vivos ante la reducción o interrupción anticipada de la lactancia. Los términos "alimentos fermentados" y "probióticos" no son sinónimos. La identidad microbiológica, la inocuidad y la existencia de estudios clínicos de eficacia para unos y otros son claves para entender sus diferencias y decidir una eventual recomendación alimentaria


Breast milk provides microorganisms that colonize the gut and program the immune system to develop oral tolerance. Between the 6 months of exclusive breastfeeding and the recommended 2 years of prolonged breastfeeding, complementary feeding leads to a progressive reduction in the entry of live microorganisms into the gut ecosystem. This is because foods in general -unlike breast milk- are devoid of microorganisms or, if present, they are often inactivated during cooking. Fermented foods and probiotics could be a valuable nutritional strategy, as they would ensure the supply of live microorganisms in the face of a reduction or early cessation of breastfeeding. The terms "fermented foods" and "probiotics" are not synonymous. Microbiological identity, safety, and the existence of clinical efficacy studies supporting both are key to understand their differences and decide on an eventual dietary recommendation


Subject(s)
Humans , Male , Female , Infant, Newborn , Infant , Fermented Foods , Yogurt , Probiotics , Microbiota , Immunity , Infant Nutritional Physiological Phenomena
6.
Rev. argent. microbiol ; 52(4): 41-50, dic. 2020. graf
Article in English | LILACS | ID: biblio-1340919

ABSTRACT

Abstract The health benefits attributed to probiotics generate interest in the search of competent strains adapted to several ecological niches, especially those related to traditional beverages and foods of each country. Pineapple tepache, a traditional Mexican fermented beverage, was used for the isolation of lactic acid bacteria with probiotic potential, one of which withstood the in vitro tests. The isolated strain AB-05, which exhibited the tested probiotic functional properties, was designated as Lactobacillus pentosus ABHEAU-05. The sequence was registered in GenBank under access code MK587617. This study is the first report of a lactic acid bacterium with in vitro digestion resistance isolated from pineapple tepache. The survival of L. pentosus ABHEAU-05 in a symbiotic medium was proven using fermented milk enriched with inulin. The in vitro digestion-resistant probiotic activity of lactobacilli was measured through analysis of pH and proteolysis. Results showed that L. pentosus grew properly in fermented milk; therefore, this microorganism could be used in the manufacture of this kind of products. The concentration of L. pentosus reached up to 8.5 logCFU/ml after 40 h of fermentation. In addition, the production of peptides and the decrease in pH indicated the vigorous and active metabolic state of the lactic acid bacterium tested. The activity and the concentration of this microorganism were maintained during refrigeration. The results of this research conclude that L. plantarum ABHEAU-05 is an in vitro digestion-resistant microorganism that can be used as a starter culture for the production of functional foods of dairy origin.


Resumen Los beneficios a la salud atribuidos a los probióticos generan interés en la búsqueda de cepas competentes adaptadas a varios nichos ecológicos, especialmente los relacionados con bebidas y alimentos tradicionales de cada país. En este estudio, se aisló del tepache de pina, una bebida fermentada tradicional mexicana, una bacteria láctica resistente a la digestión in vitro. Entre 5 bacterias aisladas, una de ellas soportó las pruebas simuladas de digestión gastrointestinal. Se analizó la resistencia a sales biliares, a condiciones ácidas y al ataque enz-imático con pepsina. La bacteria aislada, que exhibió las propiedades funcionales probióticas referidas, fue identificada como Lactobacillus pentosus y designada como L. pentosus ABHEAU-05. La secuencia fue depositada en GenBank (acceso MK587617). Se comprobó la supervivencia de L. pentosus ABHEAU-05 en una leche fermentada adicionada con inulina y su resistencia a la digestión in vitro mediante el análisis del pH y la proteólisis. Los resultados muestran que la leche fermentada es una matriz adecuada, donde L. pentosus ABHEAU-05 se desarrolla sin inconvenientes, alcanzando un título de 8,5 logufc/ml después de 40 h de fermentación. Además, la producción de péptidos y el descenso del pH indicaron el estado metabólico vigoroso y activo del microorganismo probiótico. Se concluye que L. pentosus ABHEAU-05 es un microorganismo resistente a la digestión in vitro, que puede servir como cultivo iniciador para la producción de alimentos de origen lácteo. Este es el primer informe acerca del aislamiento de una bacteria ácido láctica resistente a la digestión in vitro a partir del tepache de piña.


Subject(s)
Probiotics , Lactobacillus pentosus , Fermented Foods , Beverages , Lactic Acid , Digestion , Fermentation , Food Microbiology
7.
Rev. med. vet. zoot ; 67(3): 219-229, sep.-dic. 2020. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1251917

ABSTRACT

RESUMEN Una de las principales formas de contaminación de la leche con micotoxinas es el consumo de alimentos fermentados que se encuentran contaminados con mohos principalmente de Aspergillus spp., los cuales producen toxinas que pueden llegar a constituirse como un problema para la salud publica debido a su estabilidad térmica y química. El objetivo del presente trabajo fue detectar las concentraciones de aflatoxina M1 en muestras de leche de vacas en tanques de enfriamiento en cuatro municipios del departamento de Boyacá durante un año, determinando las variaciones de acuerdo con la temporada. Se realizó un estudio de corte longitudinal, descriptivo cuantitativo. Se seleccionaron aleatoriamente cuatro tanques de enfriamiento de cuatro municipios distintos del departamento; cada uno se muestreó dos veces al mes durante todo el período de estudio y se procesaron mediante metodología Charm Ez Lite . Se realizó un ANDEVA para determinar las diferencias estadísticas entre las concentraciones de la aflatoxina M1 por cada trimestre. Se determinaron diferencias estadísticas entre cada uno de los trimestres del estudio encontrando un porcentaje de positividad de 74,06% del total de muestras positivas en los trimestres de verano. 28,12% (108) de las muestras tomadas durante todo el estudio fueron positivas, con concentraciones de la toxina que oscilaron entre 0,5 y 2,0 μg/Kg de leche. Se determinó por primera vez en el departamento de Boyacá las concentraciones y variaciones estacionales de aflatoxina M1 en muestras de tanques de enfriamiento de leche, encontrando las mayores concentraciones y número de casos positivos de aflatoxina M1 en los meses de verano.


ABSTRACT One of the main forms of contamination of milk with mycotoxins is the consumption of fermented foods that are contaminated with mold, mainly Aspergillus spp, which produce toxins that can become a public health problem due to their thermal and chemical stability. The objective of the present work was to detect aflatoxin M1 concentrations in cows' milk samples in cooling tanks in four municipalities of the department of Boyacá for one year, determining the variations according to the season. A longitudinal, quantitative descriptive study was carried out, four cooling tanks from four different municipalities in the department were randomly selected, each tank, in each municipality, was sampled twice a month throughout the study period and processed using Charm methodology Ez Lite®, an ANDEVA was performed to determine the statistical differences between aflatoxin M1 concentrations for each quarter. Statistical differences were determined between each of the quarters of the study, finding a positivity percentage of 74.06% of the total positive samples in the summer quarters. 28.12% (108) of the samples taken throughout the study were positive, with toxin concentrations ranging between 0.5 and 2.0 μg/Kg of milk. Seasonal concentrations and variations of aflatoxin M1 in milk cooling tank samples were determined for the first time in the department of Boyacá, finding the highest concentrations and number of positive cases of aflatoxin M1 in the summer months.


Subject(s)
Animals , Cattle , Aspergillus , Seasons , Cattle , Public Health , Longitudinal Studies , Aflatoxin M1 , Milk , Food Contamination , Chemistry , Cold Temperature , Fermented Foods , Mycotoxins
8.
Rev. chil. nutr ; 47(3): 390-395, jun. 2020. tab, graf
Article in English | LILACS | ID: biblio-1126136

ABSTRACT

The aim of this research was to characterize a fermented yogurt-type milk drink that was elaborated from whey with the addition of aloe vera crystals and granadilla (Passiflora ligularis Juss). Four formulations for characterization were obtained: F1 (control), F2 (5% aloe vera and 15% granadilla), F3 (10% aloe vera and 10% granadilla) and F4 (15% aloe vera and 5% granadilla). Next, a sensory evaluation through a hedonic test was conducted to choose the formulation with the highest acceptance. After the best formulation was chosen, a bromatological and syneresis characterization was performed. The F4 sample had the best results in the sensory evaluation. Compared to the F1 sample, statistically significant differences (p<0.05) in all parameters except in total carbohydrates was observed for the bromatological analysis. In the percentage of syneresis, at the end of the 15 days, F4 presented a lower value in this parameter with respect to the control sample. It could be determined that the higher percentage of aloe vera and the lower percentage of granadilla had a big influence on the parameters evaluated, where it presented the best results compared to the other samples evaluated.


El objetivo de esta investigación fue caracterizar una bebida láctea fermentada tipo yogur que se elaboró a partir de lactosuero con la adición de cristales de aloe vera y granadilla (Passiflora ligularis Juss). Se obtuvieron cuatro formulaciones para la caracterización: F1 (control), F2 (5% de aloe vera y 15% de granadilla), F3 (10% de aloe vera y 10% de granadilla) y F4 (15% de aloe vera y 5% de granadilla). A continuación, se realizó una evaluación sensorial mediante una prueba hedónica para elegir la formulación de mayor aceptación. Una vez elegida la mejor formulación, se realizó una caracterización bromatológica y de sinéresis. La muestra F4 obtuvo los mejores resultados en la evaluación sensorial. En comparación con la muestra F1, se observaron diferencias estadísticamente significativas (p<0,05) en todos los parámetros, excepto en los carbohidratos totales. En el porcentaje de sinéresis, al final de los 15 días, F4 presentó un valor inferior en este parámetro con respecto a la muestra de control. Se pudo determinar que el mayor porcentaje de aloe vera y el menor porcentaje de granadilla influyeron mucho en los parámetros evaluados, en los que presentó los mejores resultados en comparación con las otras muestras evaluadas.


Subject(s)
Passiflora , Milk , Aloe , Food Analysis , Fermented Foods , Taste , Whey
9.
São Paulo; s.n; s.n; 2020. 75 p. graf, tab.
Thesis in Portuguese | LILACS | ID: biblio-1290802

ABSTRACT

O kombucha é uma bebida fermentada tradicional, originária da China, preparada pela fermentação de chá preto adoçado com cultura mista de bactérias e leveduras chamada Simbiotic Culture of Bacteria and Yeast (SCOBY). Tem sido alegado que o mesmo possui propriedades funcionais, tais como recuperação ou manutenção de peso corporal, atividade antihiperglicêmica, entre outras. Por não existirem estudos suficientes que as comprovem, este trabalho teve por objetivo avaliar a influência do consumo de kombucha como tratamento alternativo para amenizar e/ou retardar sintomas e complicações do Diabetes Mellitus e identificar as possíveis modificações metabólicas, morfológicas e imunológicas ocorridas em camundongos com diabetes tipo 1. De acordo com os resultados obtidos, observou-se que, apesar de ter havido recuperação de massa corpórea próxima daquela que se tinha antes da indução da diabetes, esse efeito não foi exclusivo do kombucha e, embora a influência no controle glicêmico tenha sido maior nos camundongos normoglicêmicos que diabéticos, acredita-se que a administração por um período prolongado pudesse indicar melhores resultados, uma vez que as avaliações histológicas e morfométricas do intestino demonstraram resultados satisfatórios quanto ao aumento da superfície de mucosa e diminuição do infiltrado inflamatório, favorecendo a modulação imunológica. Logo, considera-se necessária a realização de mais trabalhos para comprovação da capacidade funcional do kombucha e elucidação de sua eficácia enquanto tratamento exclusivo e/ou complementar do diabetes


Kombucha is a traditional Chinese fermented beverage prepared by fermenting sweetened black tea with mixed bacterial and yeast culture called Simbiotic Culture of Bacteria and Yeast (SCOBY). It has been claimed that it has functional properties such as body weight recovery or maintenance, antihyperglycemic activity, among others. Because there are not enough studies to prove them, this study aimed to evaluate the influence of kombucha consumption as an alternative treatment to alleviate and/or delay symptoms and complications of Diabetes Mellitus and to identify possible metabolic, morphological and immunological changes in mice with type 1 diabetes. According to the results obtained, it was observed that, although there was a recovery of body mass close to the one obtained before diabetes induction, this effect was not unique to kombucha, and although the influence on glycemic control was greater in normoglycemic rather than diabetic mice, it is believed that administration over a prolonged period could indicate better results, since histological and morphometric evaluations of the intestine showed satisfactory results in terms of mucosal surface enlargement and decreased inflammatory infiltrate, favoring immune modulation. . Therefore, further work is considered necessary to prove the functional capacity of kombucha and to elucidate its effectiveness as an exclusive and / or complementary treatment of diabetes


Subject(s)
Animals , Mice , Diabetes Mellitus, Type 1/diagnosis , Kombucha Tea/analysis , Efficacy/classification , Diabetes Complications/complications , Functional Food/analysis , Fermented Foods/adverse effects , Hypoglycemic Agents , Inflammation/prevention & control , Maintenance
10.
Prensa méd. argent ; 105(4): 197-204, jun 2019. graf, tab
Article in English | LILACS, BINACIS | ID: biblio-1045909

ABSTRACT

The article is dedicated to the development of a multicomponent high-protein product for the nutrition of physically active individuals. The possibility to introduce flavoring fillers and a biologically active supplement in the milk and vegetable base of fermented whipped dessert has been established, and the expediency thereof has been justified. Based on a mathematical model, the possibility of preparing dessert systems with the specified optimum parameters has been confirmed. The influence of nondairy protein components on the improvement of functional and technological properties of multicomponent mixtures for desserts has been revealed. It has been demonstrated that the inclusion of desserts with specified optimum parameters in the diet will have a positive effect on increasing the body's adaptive capacity.


Subject(s)
Humans , Food, Fortified , Dietary Supplements , Foods for Persons Engaged in Physical Activities , Flavoring Agents , Fermented Foods
11.
Hig. aliment ; 33(288/289): 515-519, abr.-maio 2019. tab, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1481987

ABSTRACT

O trabalho foi realizado com o objetivo de elaborar bebida alcoólica fermentada a partir da polpa de jabuticaba e determinar a atividade antioxidante desse fermentado alcoólico. A partir da polpa, foi preparado mosto e inoculado com Saccharomyces cerevisiae, CCMA 0200, o processo fermentativo foi conduzido em fermentadores com capacidade de 6 L a22˚C. As fermentações foram monitoradas diariamente e amostras foram retiradas para análises cromatográficas. Ao fim da fermentação foram analisados o teor de compostos fenólicos totais e atividade antioxidante. O teor de etanol, ao final do processo fermentativo foi de 67,3 g.L-1 e foram identificados 25 compostos voláteis. O fermentado alcoólico de jabuticaba além de apresentar compostos importantes para o sabor e o aroma da bebida, possuem antioxidantes que são fontes de substâncias bioativas.


Subject(s)
Fermented Foods , Antioxidants , Alcoholic Beverages/analysis , Myrtaceae , Wine/analysis , Chemical Phenomena , Saccharomyces cerevisiae
12.
Hig. aliment ; 33(288/289): 564-568, abr.-maio 2019. ilus, tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1481997

ABSTRACT

Alimentos funcionais são a nova tendência da indústria de alimentos, em consequência da comprovação científica das relações existentes entre alimentos e saúde e, sobretudo, do interesse do consumidor por novas alternativas saudáveis. Diante dessa realidade, promoveu-se uma ampla divulgação no uso dos probióticos para a manutenção da saúde do intestino, levando a um considerável aumento no seu consumo. No Brasil, os produtos mais populares, carreadores dessas bactérias, são os leites fermentados. O Kefir apresenta as mesmas características funcionais dos probióticos, mas de custo reduzido. O objetivo deste trabalho foi desenvolver uma bebida à base de extrato de arroz fermentado com kefir não lácteo. A análise sensorial mostrou que a bebida obteve 93,44% de aceitação e 65,57% de intenção de compra pelos provadores. Conclui-se que é viável o desenvolvimento e a comercialização da bebida fermentada funcional a base de extrato de arroz, podendo oferecer benefícios adicionais à saúde dos consumidores.


Subject(s)
Humans , Functional Food , Consumer Behavior , Kefir , Oryza , Probiotics , Fermented Foods , Beverages
13.
Hig. aliment ; 33(288/289): 579-583, abr.-maio 2019. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1482000

ABSTRACT

Objetivou-se analisar o comportamento fermentativo da cultura Lactobacillus plantarum CNPC 003 em leite de cabra em quatro tratamentos por 6 horas: T1 – L. plantarum CNPC 003; T2 – L. plantarum + oliogofrutose (FOS); T3 – L. plantarum + Streptococcus thermophilus; T4 – com L. plantarum + S. thermophilus + FOS. Os tratamentos estudados diferiram significativamente entre si em relação à acidez e ao pH após 6h de fermentação (p<0,05), tendo sido verificada uma influência positiva do uso de S. thermophilus e da adição de FOS sobre esses parâmetros. Do mesmo modo, a presença de FOS melhorou a viabilidade dos microrganismos estudados após 6h de fermentação. A utilização de S. thermophilus e de FOS é uma alternativa viável para o uso em leites fermentados contendo a cepa nativa L. plantarum CNPC 003.


Subject(s)
Animals , Lactobacillus plantarum/chemistry , Milk/chemistry , Oligosaccharides/administration & dosage , Oligosaccharides/adverse effects , Streptococcus thermophilus , Acidification , Fermented Foods , Goats
14.
Hig. aliment ; 33(288/289): 1304-1308, abr.-maio 2019. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1482150

ABSTRACT

Bebidas vegetais são alternativas para pessoas com alergias e intolerâncias ao leite de vaca, além de serem também consumidas pela parte da população que opta pela exclusão de leites e derivados. Em paralelo, o consumo de alimentos com propriedades probióticas está em ascensão e o Kefir apresenta uma forma artesanal na obtenção desses. Assim, o objetivo deste trabalho foi avaliar os parâmetros físico-químicos das bebidas vegetais antes e após o processo fermentativo pelo Kefir. Os parâmetros físico-químicos determinados foram: umidade, cinzas, proteínas, lipídios totais e carboidratos, pH, sólidos solúveis totais e cor. Com os resultados foi possível verificar que a determinação dos parâmetros físico-químicos é necessária para uma melhor compreensão do comportamento dos micro-organismos presentes nos processos fermentativos das bebidas vegetais para avaliar a viabilidade do produto elaborado.


Subject(s)
Fermented Foods/analysis , Plant Extracts/analysis , Chemical Phenomena , Kefir , Probiotics , Fruit and Vegetable Juices/analysis
15.
Hig. aliment ; 33(288/289): 1324-1328, abr.-maio 2019. ilus, tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1482154

ABSTRACT

O trabalho consistiu na elaboração de uma bebida alcoólica fermentada de muruci, e caracterizar os seus parâmetros físico-químicos. Para isso foram elaboradas três formulações com concentrações de polpas a 5, 15 e 25%. A fermentação foi conduzida à temperatura ambiente com acompanhamento diário da acidez e pH durante 15 dias, sendo o teor alcoólico analisado ao término da fermentação. Nos produtos finais foram analisados: pH (3,33; 3,42; 3,1); glicídios totais (57,87; 48,14; 41,20%); acidez total (33,97; 40,18; 95,39 meq/L); extrato seco (77,37; 57,65; 49,29%); densidade (1,017; 1,010; 1,005) e teor alcoólico (7,6; 8,9; 9,7 %v/v). Os resultados obtidos mostraram boa adequação do fruto para a produção de bebida alcoólica fermentada.


Subject(s)
Fermented Foods/analysis , Alcoholic Beverages/analysis , Chemical Phenomena , Malpighiaceae , Food Production , Saccharomyces cerevisiae
16.
Hig. aliment ; 33(288/289): 2230-2234, abr.-maio 2019. tab, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1482305

ABSTRACT

A soja apresenta propriedades benéficas à saúde como a redução dos níveis de colesterol e efeitos protetores contra a obesidade. Os indivíduos intolerantes à lactose podem utilizar produtos como a bebida fermentada de soja como alternativa em substituição ao leite de vaca. Diante deste contexto, este estudo almejou o desenvolvimento de uma bebida fermentada à base de soja adicionada de fibra dietética solúvel como a inulina e saborizada com polpa de goiaba. Os resultados da avaliação microbiológica das formulações estavam de acordo com os parâmetros vigentes na legislação brasileira, denotando que as boas práticas na sua produção estavam adequadas. Na análise de minerais observou-se que os teores de potássio (629mg à 652mg), ferro (1,44mg à 1,56mg) e fósforo (323mg à341,33mg) se apresentaram acima da quantidade presente no leite de vaca. Este produto desenvolvido pode ser uma alternativa saudável e atender à expectativa de consumidores intolerantes à lactose, quanto à saúde e bem-estar.


Subject(s)
Soy Foods/analysis , Soy Foods/microbiology , Food Microbiology , Nutritive Value , Fermented Foods , Food, Formulated , Inulin
17.
China Journal of Chinese Materia Medica ; (24): 4015-4020, 2019.
Article in Chinese | WPRIM | ID: wpr-1008320

ABSTRACT

The present work was launched to study the chemical constituents from fermented Noni juice. Ten compounds were isolated by using silicagel,Sephadex LH-20 and pre-HPLC. The structures of these compounds were identified by 1D and 2D NMR. Their structures were established as norimester( 1),cyclo( R-Pro-S-Phe)( 2),cyclo-( L-Pro-L-Leu)( 3),trans-cyclo-( D-tryptophanyl-Ltyrosyl)( 4),cyclo( L-Trp-N-methyl-L-Ala-)( 5),4-allyl-2-hydroxyphenyl 1-O-β-D-apiosyl-( 1 → 6)-β-D-glucopyranoside( 6),2-methoxy-4-vinylphenyl β-D-apiofuranosyl-( 1→6)-β-D-glucopyranoside( 7),( +)-lirioresinol A( 8),( ±)-syringaresinol( 9),and2-formyl-1 H-pyrrole-1-butanoic acid( 10). All compounds were tested for their antibacterial activity and α-glucosidase inhibitory activity. Compound 1 was a new compound. All compounds were isolated from fermented Noni juice for the first time. Compound 1 showed significant inhibitory activity against α-glucosidase [IC50 value of( 4. 22±0. 03) μmol·L-1].


Subject(s)
Chromatography, High Pressure Liquid , Fermented Foods/analysis , Fruit and Vegetable Juices/analysis , Magnetic Resonance Spectroscopy , Morinda/chemistry , Phytochemicals/analysis
18.
Environmental Health and Preventive Medicine ; : 25-25, 2019.
Article in English | WPRIM | ID: wpr-777616

ABSTRACT

BACKGROUND@#The dietary pattern of pregnant women is known to be associated with preterm birth (PTB). We investigated whether PTB was associated with intake of fermented food by using data from the Japan Environment and Children's Study.@*METHODS@#From a data set of 103,099 pregnancies, 77,667 cases at low risk for PTB were analyzed. The primary outcome measurements were based on PTB. Fermented food (miso soup, yogurt, cheese, and fermented soybeans) consumption was assessed by using a semi-quantitative food frequency questionnaire.@*RESULTS@#Intake of miso soup, yogurt, and fermented soybeans before pregnancy significantly reduced the risk of early PTB (< 34 weeks). The adjusted odds ratio (OR) for early PTB in women who had miso soup 1-2 days/week, 3-4 days/week, or ≥ 5 days/week were 0.58, 0.69, and 0.62, respectively, compared with those who had miso soup < 1 day/week (95% confidence interval (CI) 0.40-0.85, 0.49-0.98, and 0.44-0.87). The adjusted OR for early PTB in women who ate yogurt ≥ 3 times/week was 0.62 (95% CI, 0.44-0.87) compared to those who ate yogurt < 1 time/week. The adjusted OR for early PTB in women who ate fermented soybeans ≥ 3 times/week was 0.60 (95% CI, 0.43-0.84) compared to those who ate < 1 time/week. However, the incidence of overall PTB and late PTB (34-36 weeks) was not associated with fermented food intake.@*CONCLUSION@#PTB low-risk women with a high consumption of miso soup, yogurt, and fermented soybeans before pregnancy have a reduced risk of early PTB.


Subject(s)
Adult , Female , Humans , Pregnancy , Cohort Studies , Diet , Feeding Behavior , Fermented Foods , Gestational Age , Japan , Epidemiology , Odds Ratio , Premature Birth , Epidemiology , Protective Factors , Surveys and Questionnaires
19.
Chinese Journal of Applied Physiology ; (6): 130-133, 2018.
Article in Chinese | WPRIM | ID: wpr-773788

ABSTRACT

OBJECTIVES@#To investigate the effect of two kinds of fermented food, noni juice and natto, on blood glucose and lipids in induced diabetic mice.@*METHODS@#Female (ICR) mice were induced into diabetes by an injection of alloxan (55 mg/kg, i. v.). After 72 hours, those mice whose fasting blood glucose levels were over 12.00 mmol/L and urine sugar was strongly positive (+ + +) were regarded as diabetical model and were randomly divided into three groups (=10):diabetical model (DM) group, noni juice (NJ) group and natto (NT) group. Another ten normal female ICR mice were taken as normal control (NC) group. The mice in NJ and NT groups were gavaged with noni juice (25.0 ml/kg) and natto (0.6 g/kg) respectively. The other two groups were given normal saline (25.0 ml/kg). Continuous gavage administration was given for 30 days, the water-drinking volume and food-intake were recorded. After 1.5 h of the last administration, the glucose tolerance of mice was measured. Finally, the changes in glycated serum protein(GSP), insulin(Ins) and blood lipids of blood samples of mice, taken from the femoral artery, were determined.@*RESULTS@#Compared to the NC group, the water-drinking amount and food-intake, GSP and total cholesterol (TC), triglyceride(TG) and low density lipoprotein (LDL) in DM group were increased significantly (<0.01), while glucose tolerance, Ins and high density lipoprotein (HDL) were decreased significantly (<0.01). However, when it came to DM group, NJ and NT could significantly (<0.01 or <0.05) reduce, GSP, TG and LDL, meanwhile improve glucose tolerance, Ins and HDL (<0. 01).@*CONCLUSIONS@#Both noni juice and natto could reduce the blood glucose levels in induced diabetical mice and improve blood lipids, which suggested that they may have certain application value in prevention and treatment of diabetic mellitus.


Subject(s)
Animals , Female , Mice , Blood Glucose , Diabetes Mellitus, Experimental , Blood , Fermented Foods , Fruit and Vegetable Juices , Lipids , Blood , Mice, Inbred ICR , Random Allocation
20.
Food Sci. Technol (SBCTA, Impr.) ; 38(2): 299-309, Apr.-June 2018. tab, graf
Article in English | LILACS | ID: biblio-999781

ABSTRACT

Fermented beverages are widely used all over the country. Fermented plant beverages (FPB) are prevalent in Thailand and FPBs are believed to cure and prevent many health oriented problems. The people of Thailand produce many varieties of FPBs in small scale or large scale and consume them in their daily lives. This study is a survey conducted among the representative consumers of FPBs in Thailand to know the consumer's opinion on FPBs, effects and benefits of FPBs, and real status of consumer satisfaction in Thailand. This study revealed that the rationale for the consumption of respective FPBs was to treat their health issues and for the betterment of their health. Most of the consumers of FPBs benefited in case of improving their physical and mental health. The current survey revealed the opinion of the FPBs consumers in Thailand. This study concluded that FPBs are health promoting drink that is affordable in the daily life of Thai people. The FPBs prepared in Thailand did not report any massive adverse effects in Thailand. Till now the preparation and consumption of FPBs are followed in Thailand and not influenced by adverse effects; FPBs are considered safe for human consumption.


Subject(s)
Humans , Male , Female , Thailand , Fermented Foods , Health Promotion , Beverages , Mental Health , Interview , Consumer Behavior
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