Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 98
Filter
1.
Int. j. odontostomatol. (Print) ; 14(1): 12-18, mar. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1056495

ABSTRACT

RESUMEN: La caries dental no tratada es la enfermedad crónica más frecuente en adultos y la caries no tratada en dientes temporales es la décima enfermedad crónica más frecuente. En Chile los adultos presentan un COPD promedio entre 3,76 - 22,16, pero los estudios no son en población representativa y no existen estudios comparativos entre ceod/ COPD e ICDASTM. A nivel mundial, su prevalencia puede alcanzar el 100 % de la población estudiada. Se buscará evaluar si existen diferencias en la sensibilidad y especificidad en la detección de caries utilizando ICDASTM y ceod/COPD. Se realizó una revisión sistemática cualitativa realizando una búsqueda sobre los índices de caries ceod/COPD y sistema ICDASTM, tanto en Chile como a nivel mundial, en las bases de datos Medline, ClinicalKey y SciELO encontrando 3.581, de ellos, 21 se leyeron por completo y 14 cumplieron nuestros criterios. La sensibilidad y especificidad es mayor con ICDASTM que con el índice ceod/COPD y entrega hasta un 43 % más información al detectar lesiones no cavitadas, pero necesita de mayor tiempo para su realización y más recursos al utilizar luz, aire comprimido y una profilaxis previa al examen. Capacitaciones previas, conocimientos y experiencia en ICDASTM son fundamentales para el mejor resultado, estudios demostraron que mientras mayor experiencia, la sensibilidad y especificidad aumentaba a diferencia del índice ceod/COPD. Se deben realizar más estudios comparativos entre ambos índices e incluir ICDASTM en niños por su capacidad de detectar lesiones no cavitadas las cuales son las más prevalentes en dientes temporales.


ABSTRACT: Untreated dental caries is the most frequent chronic disease in adults and untreated caries in primary teeth is the tenth most frequent chronic disease. In Chile, adults have an average DMFT between 3.76-22.16, but the studies are not in a representative population and there are no comparative studies between dmft/DMFT and ICDASTM. Worldwide, its prevalence can reach 100 % of the population studied. The objective of the study was to evaluate if there are differences in the sensitivity and specificity in the detection of caries using ICDASTM and dmft/DMFT. A qualitative systematic review was done, carrying out a search on the dmft/DMFT index and the ICDASTM system, both in Chile and worldwide, in the Medline, ClinicalKey and SciELO databases, finding 3,581, of which, 21 were read completely and 14 met the inclusion and exclusion criteria. The sensitivity and specificity is greater with ICDASTM than with the dmft/DMFT index and delivers up to 43 % more information when detecting non-cavitated lesions, but it needs more time for its realization and more resources because it needs to use light, compressed air and prophylaxis before the examination. Previous training, knowledge and experience in ICDASTM are fundamental for the best results. Studies showed that the greater the experience, the sensitivity and specificity increased, unlike the dmft/DMFT index. More comparative studies should be conducted between both indexes and include ICDASTM in children for their ability to detect non-cavitated lesions, which are the most prevalent in temporary teeth.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Photochemotherapy/methods , DMF Index , Dental Caries/diagnostic imaging , Time Factors , Photosensitizing Agents , Dental Caries Susceptibility
2.
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 12: 505-509, jan.-dez. 2020. tab
Article in English, Portuguese | LILACS, BDENF | ID: biblio-1097201

ABSTRACT

Objetivo: avaliar a eficácia antimicrobiana da terapia fotodinâmica no tratamento da candidíase oral em pessoas vivendo com HIV/aids. Método: estudo experimental, qualitativo e descritivo com 18 pessoas vivendo com HIV/aids que manifestavam a candidíase oral, maiores de 18 anos, que estavam em tratamento no Hospital Universitário Gaffrée e Guinle. Este grupo subdividiu-se em um grupo controle, composto por sete pessoas, que recebeu tratamento com a terapia fotodinâmica e antifúngicos, e um grupo experimental, com 11, que recebeu apenas a terapia fotodinâmica. A evolução do tratamento de cada participante foi acompanhada por registros fotográficos em duas consultas, inicial e final. Esta pesquisa foi aprovada pelo Comitê de Ética em Pesquisa do hospital, parecer número 2.431.107. Resultados: a maioria dos participantes apresentou melhora clínica, ainda que discreta, e em apenas um houve piora clínica. Conclusão: a terapia fotodinâmica antimicrobiana pode ser eficaz no tratamento da candidíase oral em pessoas vivendo com HIV/Aids


Objective: evaluate the antimicrobial efficacy of photodynamic therapy in the treatment of oral candidiasis in people living with HIV/ aids. Method: experimental, qualitative and descriptive study with 18 people living with HIV/aids who presented oral candidiasis, over 18 years of age, who were being treated at the Gaffrée and Guinle University Hospital. This group was subdivided into a control group, composed of seven people, who received treatment with photodynamic and antifungal therapy, and an experimental group, with 11, who received only the photodynamic therapy. The evolution of the treatment of each participant was followed by photographic registers in two appointments, initial and final. This research was approved by the Research Ethics Committee from the hospital, dictum number 2.431.107. Results: most of the participants showed clinical improvement, albeit discrete, and in only one there was clinical worsening. Conclusion: antimicrobial photodynamic therapy may be effective in the treatment of oral candidiasis in people living with HIV/Aids


Objetivo: evaluar la eficacia antimicrobiana de la terapia fotodinámica en el tratamiento de la candidiasis bucal en personas que viven con VIH/sida. Método: estudio experimental, cualitativo y descriptivo con 18 personas viviendo con VIH/sida que manifestaban la candidiasis bucal, mayores de 18 años, que estaban en tratamiento en el Hospital Universitario Gaffrée y Guinle. Este grupo se subdividió en grupo control, compuesto por siete personas, que recibió tratamiento con la terapia fotodinámica y antifúngicos, y un grupo experimental, con 11, que recibió sólo la terapia fotodinámica. La evolución del tratamiento de cada participante fue acompañada por registros fotográficos en dos consultas, inicial y final. La investigación fue aprobada por el Comité de Ética en Investigación del lugar, dictamen número 2.431.107. Resultados: la mayoría de los participantes presentó mejoría clínica, aunque discreta, y en apenas uno hubo empeoramiento clínico. Conclusión: la terapia fotodinámica antimicrobiana puede ser eficaz en el tratamiento de la candidiasis bucal en personas que viven con el VIH/Sida


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Photochemotherapy/statistics & numerical data , Candidiasis, Oral/therapy , AIDS-Related Opportunistic Infections/therapy , Photochemotherapy/methods , Candidiasis, Oral/epidemiology , Acquired Immunodeficiency Syndrome/therapy , HIV , Drug Resistance, Fungal
3.
Braz. oral res. (Online) ; 33: e092, 2019. tab, graf
Article in English | LILACS | ID: biblio-1039296

ABSTRACT

Abstract This study evaluated the effect of antimicrobial photodynamic therapy (aPDT) on the endodontic treatment of apical periodontitis (AP). AP was induced in 48 premolars of 6 dogs. After biomechanical preparation, the teeth were divided into 4 groups: Calcium-Hydroxide (CH)/120d and CH/180d: root canals filled with CH-based dressing for 15 days before obturation; aPDT/120d and aPDT/180d: conditioning with phenothiazine photosensitizer (10 mg/mL) for 1 minute and irradiation with diode laser in the same session as obturation. Root filling was performed with AH Plus sealer. After the experimental periods, animals were euthanized and teeth were submitted for histology. HE staining was performed for descriptive analysis of the periapical region, measurement of apical periodontitis and for inflammatory cells, and blood vessels count. Immunohistochemistry was performed for osteopontin (OPN) and alkaline phosphatase (ALP). Data were analyzed statistically by two-way ANOVA and chi-square test (α = 5%). Teeth in Group CH/120d presented only a slightly enlarged periodontal ligament (PL) with advanced repair. Group aPDT/120d presented the PL moderately enlarged, with moderate inflammatory infiltrate and few collagen fibers. The same pattern was observed at 180 days. AP lesions in CH-treated groups were smaller than those in aPDT-treated groups (p < 0.001) with more blood vessels (p < 0.0001), regardless of the evaluation period, without significant differences in the number of inflammatory cells (p > 0.05). CH-treated groups showed significantly more intense immunostaining for ALP and OPN (p < 0.001) in both periods. Although aPDT stimulated angiogenesis and expression of bone formation markers, the two-session endodontic treatment with CH-based dressing promoted better apical periodontitis repair.


Subject(s)
Animals , Periapical Periodontitis/drug therapy , Photochemotherapy/methods , Root Canal Therapy/methods , Bone Cements/therapeutic use , Calcium Hydroxide/therapeutic use , Periapical Periodontitis/pathology , Time Factors , Bone Regeneration/drug effects , Immunohistochemistry , Reproducibility of Results , Treatment Outcome , Neovascularization, Physiologic/drug effects , Evaluation Study
4.
Braz. oral res. (Online) ; 33(supl.1): e071, 2019. graf
Article in English | LILACS | ID: biblio-1039316

ABSTRACT

Abstract The aim of this illustrated review is to present the new strategies and developments to treatment and diagnosis of periimplant diseases. Periimplant disease is a subject of great concern for modern dentistry. The numbers of implant exhibiting biological complications grows as implant dentistry expands thought-out the world. Diagnosis and treatment of those diseases are still controversial and difficult. We present novel treatment for infection control and biological rationale of additional use of guided bone regeneration, with an illustrative explanation of the treatments presented with two cases.


Subject(s)
Humans , Photochemotherapy/methods , Peri-Implantitis/diagnosis , Peri-Implantitis/therapy , Bone Regeneration , Dental Implants/adverse effects , Reproducibility of Results , Treatment Outcome , Guided Tissue Regeneration, Periodontal/methods , Bone-Anchored Prosthesis/adverse effects
5.
An. bras. dermatol ; 93(4): 539-545, July-Aug. 2018. tab, graf
Article in English | LILACS | ID: biblio-949933

ABSTRACT

Abstract: Background: To study the effective therapeutic concentration, drug application duration, irradiation duration and irradiation dosage of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) for treating vitiligo and observe its clinical efficacy. Objective: To assess the clinical efficacy of ALA-PDT for treating vitiligo. Methods: ALA with different drug concentrations, drug application durations, irradiation durations and irradiation dosages were adopted to treat skin lesions caused by vitiligo to determine the effective drug concentration, drug application duration, irradiation duration and irradiation dosage for treating three vitiligo volunteers and observe the therapeutic results. Results: The clinical trial suggested that ALA at a drug concentration of 1.5%, drug application duration for 3 hours, irradiation dosage of 80mw/cm2 and irradiation duration for 20 min was effective in treating vitiligo. Under these parameters, ALA-PDT was effective to the three vitiligo volunteers, with mild pain and feeling of burning but no other adverse reaction during treatment. Study limitations: Due to the small sample size in this study for the effectiveness of PDT in treating vitiligo and the potential variations in the efficacy for treating the disease at different areas, further studies shall be conducted for confirmation. Conclusions: ALA with a drug concentration at 1.5%, drug application duration for 3 hours, irradiation dosage of 80 mw/cm2 and irradiation duration for 20 min is effective in treating vitiligo. Therefore, ALA-PDT is safe and effective in treating the disease, with minor adverse events, providing a new method for treating vitiligo in the future.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Young Adult , Photochemotherapy/methods , Vitiligo/drug therapy , Photosensitizing Agents/administration & dosage , Levulinic Acids/administration & dosage , Pilot Projects , Treatment Outcome
6.
An. bras. dermatol ; 93(2): 238-241, Mar.-Apr. 2018. tab, graf
Article in English | LILACS | ID: biblio-887175

ABSTRACT

Abstract: Background: Topical agents used in combination with phototherapy or photochemotherapy may have both blocking or enhancing effects in ultraviolet rays. Objective: In this in vivo study, the effects of topical petrolatum, basis cream, glycerine, and olive oil on the transmission of ultraviolet A radiation were investigated. Methods: A test was performed to determine the minimal phototoxic dose on 29 volunteers with only psoralen plus ultraviolet A (PUVA) and then the same test was repeated with white petrolatum, basis cream, glycerine, olive oil, and sunscreen (0.3cc/25cm2). The effects of each agent on the minimal phototoxic dose were determined after 72 h. Results: When compared to pure PUVA, there was a statistically significant increase in the mean minimal phototoxic dose values by the application of white petrolatum (P = 0.011), but there was no significant increase or decrease in the mean minimal phototoxic dose values after the application of basis cream (P = 0.326), glycerine (P = 0.611) or olive oil (P = 0.799). Study limitations: Low number of patients Conclusion: The application of white petrolatum, which has a blocking effect, and also of basis cream immediately before PUVA therapy should not be recommended. Although we specify that glycerine and maybe olive oil can be used before photochemotherapy, there is a need for further research in larger series.


Subject(s)
Humans , Petrolatum/pharmacology , Photochemotherapy/methods , PUVA Therapy/methods , Skin Diseases/drug therapy , Ultraviolet Rays , Photosensitizing Agents/pharmacology , Emollients/pharmacology , Sunscreening Agents/pharmacology , Time Factors , Skin Tests , Single-Blind Method , Reproducibility of Results , Treatment Outcome , Dermatitis, Phototoxic/prevention & control , Statistics, Nonparametric , Dose-Response Relationship, Radiation , Olive Oil/pharmacology , Glycerol/pharmacology
7.
J. appl. oral sci ; 26: e20170172, 2018. tab, graf
Article in English | LILACS | ID: biblio-893733

ABSTRACT

Abstract Background: Osteoradionecrosis of the jaw (ORNJ) is the most severe and complex sequel of head and neck radiotherapy (RT) because of the bone involved, it may cause pain, paresthesia, foul odor, fistulae with suppuration, need for extra oral communication and pathological fracture. We treated twenty lesions of ORNJ using low-level laser therapy (LLLT) and antimicrobial photodynamic therapy (aPDT). The objective of this study was to stimulate the affected area to homeostasis and to promote the healing of the oral mucosa. Methods: We performed aPDT on the exposed bone, while LLLT was performed around the bone exposure (red spectrum) and on the affected jaw (infrared spectrum). Monitoring and clinical intervention occurred weekly or biweekly for 2 years. Results: 100% of the sample presented clinical improvement, and 80% presented complete covering of the bone exposure by intact oral mucosa. Conclusion: LLLT and aPDT showed positive results as an adjuvant therapy to treat ORNJ.


Subject(s)
Humans , Male , Female , Adult , Aged , Osteoradionecrosis/therapy , Photochemotherapy/methods , Jaw Diseases , Low-Level Light Therapy/methods , Chemoradiotherapy, Adjuvant/methods , Anti-Infective Agents/therapeutic use , Osteoradionecrosis/pathology , Time Factors , Wound Healing/radiation effects , Jaw Diseases/pathology , Prospective Studies , Reproducibility of Results , Treatment Outcome , Dose-Response Relationship, Radiation , Homeostasis/drug effects , Homeostasis/radiation effects , Middle Aged , Mouth Mucosa/drug effects , Mouth Mucosa/radiation effects
8.
An. bras. dermatol ; 92(5): 622-625, Sept.-Oct. 2017. tab, graf
Article in English | LILACS | ID: biblio-887046

ABSTRACT

Abstract: Background: Photodynamic therapy is a therapeutic modality that has consolidated its activity in the photooxidation of organic matter, which arises from the activity of reactive oxygen species. Objective: To evaluate the effect of red laser 660nm with the photosensitizer methylene blue on Propionibacterium acnes in vitro. Method: The experimental design was distributed into four groups (1 - control group without the application of light and without photosensitizer, 2 - application of light, 3 - methylene blue without light, and 4 - methylene blue with light). Tests were subjected to red laser irradiation 660nm by four cycles of 5 minutes at 3-minute intervals. Results: It was evidenced the prominence of the fourth cycle (20 minutes) groups 2, 3 and 4. Study limitations: Despite the favorable results, the laser irradiation time photosensitizer associated with methylene blue were not sufficient to to completely inhibit the proliferation of bacteria. Conclusion: Further studies in vitro are recommended to enable the clinical application of this photosensitizer in photodynamic therapy.


Subject(s)
Humans , Photochemotherapy/methods , Propionibacterium acnes/drug effects , Photosensitizing Agents/pharmacology , Methylene Blue/pharmacology , Propionibacterium acnes/radiation effects , Time Factors
9.
J. appl. oral sci ; 25(4): 396-403, July-Aug. 2017. tab, graf
Article in English | LILACS | ID: biblio-893636

ABSTRACT

Abstract Objective The aim of this study was to evaluate the effect of photodynamic therapy (PDT) and non-thermal plasma (NTP) on adhesion and sealer penetration in root canals. Material and Methods Sixty single-rooted premolars were used. The teeth were prepared using a crown-down technique. NaOCl and EDTA were used for irrigation and smear layer removal, respectively. The root canals were divided into three groups: control, PDT, and NTP. After treatments, the roots were filled using gutta-percha and either AH Plus (AHP) or MTA Fillapex (MTAF) sealers. Samples were sectioned at 4, 8, and 12 mm from the apex (1-mm slices)and analyzed by the push-out bond strength test (adhesion) and confocal laser scanning microscopy (sealer penetration). Data were statistically evaluated using Kruskal-Wallis, Dunn's, and Spearman's tests. Results Regarding AHP, bond strength was similar in the NTP group and in the control group, but significantly lower in the PDT group. As to MTAF, both therapies showed lower values than the control group. In the confocal analysis of AHP, maximum and mean penetration, and penetrated area were statistically higher in the control group than in the PDT and NTP groups. Penetrated perimeter was similar among groups. Regarding MTAF, all parameters yielded better results in the NTP than in the control group. The PDT and control groups showed similar results except for penetrated area. Conclusion PDT and plasma therapy affected the adhesion and sealer penetration of root canals filled with AH Plus and MTA Fillapex and there is no positive correlation between adhesion and sealer penetration.


Subject(s)
Humans , Aluminum Compounds/chemistry , Calcium Compounds/chemistry , Dental Pulp Cavity/drug effects , Epoxy Resins/chemistry , Oxides/chemistry , Photochemotherapy/methods , Plasma Gases/chemistry , Root Canal Filling Materials/chemistry , Silicates/chemistry , Dental Bonding/methods , Drug Combinations , Materials Testing , Microscopy, Confocal , Reference Values , Reproducibility of Results , Root Canal Obturation/methods , Statistics, Nonparametric
10.
An. bras. dermatol ; 92(3): 350-355, May-June 2017. tab, graf
Article in English | LILACS | ID: biblio-886948

ABSTRACT

Abstract Background: Photodynamic therapy with topical aminolevulinic acid (ALA-PDT) has been suggested to be effective in treatment of acne vulgaris. However, adverse events occur during and after treatment. Objectives: To compare the efficacy and tolerability of optical intra-tissue fiber irradiation (OFI) ALA-PDT versus traditional ALA-PDT in treatment of acne vulgaris in rabbit models. Methods: Twenty-five rabbits of clean grade were used. Twenty rabbits were randomly selected to establish acne model and the other five were used as control. Rabbits in model group (40 ears) were further divided into four groups (10 ears/group): I, OFI-ALA-PDT with the head of optical fiber inserted into the target lesion (intra-tissue); II, traditional ALA-PDT group; III, OFI group; IV, blank control group without any treatment. Uncomfortable symptoms, adverse events, and effectiveness rates were recorded on post-treatment day 14, 30, and 45. Results: On post-treatment day 14, the effectiveness rate in OFI-ALA-PDT group was obviously higher than that of the other three groups (P<0.05). However, no improved effects were observed in OFI-ALA-PDT group on day 30 and 45. During the period of treatment, the frequencies of uncomfortable symptoms in ALA-PDT group were obviously higher than those in the other three groups (P<0.05). The adverse event rate in OFI-ALA-PDT group was obviously lower than that of the ALA-PDT group (P<0.05) Study limitations: The unblindness of the study and temporary animal models of acne induced may hamper the assessment and monitoring of the results, and future studies are still needed to clarify it further. Conclusion: The OFI-ALA-PDT group (intra-tissue irradiation) showed no improved efficacy on treating rabbit ear acne but had higher safety and better tolerability.


Subject(s)
Animals , Rabbits , Photochemotherapy/methods , Photosensitizing Agents/administration & dosage , Acne Vulgaris/therapy , Aminolevulinic Acid/administration & dosage , Photochemotherapy/adverse effects , Random Allocation , Treatment Outcome , Photosensitizing Agents/adverse effects , Disease Models, Animal , Aminolevulinic Acid/adverse effects
12.
An. bras. dermatol ; 92(1): 142-144, Jan.-Feb. 2017. graf
Article in English | LILACS | ID: biblio-1038244

ABSTRACT

ABSTRACT Daylight photodynamic therapy has been used in countries with high latitudes during the summer for actinic keratoses treatment with reports of similar efficacy to conventional photodynamic therapy. We evaluate its safety in 20 patients in the city of Fortaleza, a local with low latitude and high brightness. Sixteen patients did not report any discomfort due to the procedure. Daylight photodynamic therapy is an easy application method with great tolerability by the patient and has the possibility of being performed throughout the year in these regions. It can mean a promising tool in the control of skin cancer.


Subject(s)
Humans , Photochemotherapy/methods , Scalp Dermatoses/drug therapy , Sunlight , Facial Dermatoses/drug therapy , Keratosis, Actinic/drug therapy , Time Factors , Treatment Outcome , Photosensitizing Agents/therapeutic use , Dose-Response Relationship, Radiation , Aminolevulinic Acid/analogs & derivatives , Aminolevulinic Acid/therapeutic use
13.
Bauru; s.n; 2017. 125 p. ilus, tab, graf.
Thesis in Portuguese | LILACS, BBO | ID: biblio-879882

ABSTRACT

Este estudo teve o objetivo de comparar os efeitos da biomodificação radicular por ácido cítrico associado à tetraciclina (AC) ou terapia fotodinâmica antimicrobiana (aPDT) no recobrimento de recessões gengivais pela técnica do enxerto de tecido conjuntivo subepitelial (ETCS). Para o estudo paralelo foram selecionados 60 sítios em 17 pacientes com recessões de classe I e II de Miller de 2 a 5 mm, múltiplas e isoladas, as quais foram divididas em 3 grupos: grupo controle (C) - apenas raspagem, grupo AC ­ raspagem e, aplicação de gel de ácido cítrico associado à tetraciclina (90s) e o grupo aPDT - raspagem e aplicação da terapia fotodinâmica antimicrobiana (Azul de toluidina O 100µg/ml + laser vermelho). Os parâmetros clínicos profundidade de sondagem (PS), nível clínico de inserção (NCI), altura da recessão (AR), largura mucosa ceratinizada (LMC), espessura de mucosa ceratinizada (EMC), índice de sangramento à sondagem (ISS), índice de placa (IPI) e porcentagem de recobrimento radicular (%REC) foram avaliados por um examinador cego e calibrado no baseline e 3, 6 e 12 meses de pós-operatório. Avaliou-se a hiperestesia dentinária (HIPER) e a percepção estética (EST) do paciente através de escala analógica visual no baseline e após 7 e 14 dias e 1, 3, 6 e 12 meses. A análise estatística dos dados de NCI foi feita pelo teste ANOVA complementado por Tukey. O teste de Friedman complementado por Wilcoxon foi aplicado para análise intra-grupos. O teste de Kruskal-Wallis foi aplicado para as diferenças entre os grupos, complementado por comparações múltiplas entre os três grupos. Para a %REC foi realizado o teste de Kruskal-Wallis complementado por Dunnet (p<0,05). Observou-se ganho do NCI em todos os grupos, mas este ganho foi maior para os grupos AC (0,55±1,68) e aPDT (0,80±2,11) em relação ao C (2,50±1,99) (p<0,05). Houve redução na AR em todos os grupos, porém o grupo AC (0,40±0,62) teve maior redução quando comparado C (1,15±1,04) (p<0,05). Houve aumento na LMC em todos os grupos e aumento na EMC também em todos os grupos, sendo que a EMC nos grupos aPDT (2,80±0,733) e AC (2,33±0,60) foram iguais com melhores resultados que o C (1,99±0,65) (p<0,05). Para a PS não foram observadas diferenças significantes entre os grupos e nem entre os períodos (p>0,05). A %REC foi maior nos grupos AC (82%±30%) e aPDT (82%±28%) do que no grupo C (58%±40%) (p<0,05). De maneira geral C, AC e aPDT foram semelhantes entre si e entre eles ao longo do tempo para o ISS e IPI (p>0,05). Houve redução significante da HIPER ao longo do tempo e entre os grupos, sendo que em AC (1,20±2,23) e aPDT (0,70±1,15) a diminuição da sensibilidade foi maior do que em C (2,63±2,22) (p<0,05). Houve melhora na EST ao longo do tempo e para o grupo AC (9,40±0,96) foi maior do que o C (8,58±1,07) (p<0,05), sendo que o grupo aPDT foi igual ao C. Concluiu-se que o uso de biomodificadores radiculares, como o AC e aPDT favorecem o recobrimento de recessões, resultando em redução da hipersensibilidade dentinária, maior porcentagem de recobrimento radicular e mais sítios com recobrimento completo, mantendo os resultados a longo prazo. (AU)


The aim of this study was to compare root biomodification by a combination of citric acid with tetracycline (CA) or antimicrobial photodynamic therapy (aPDT) on root coverage by subepithelial connective tissue graft (SCTG). Sixty sites with Miller's class I or II recessions were divided in three groups: Control (C) ­ scaling only, CA ­ scaling and root conditioning with a combination of citric acid and tetracycline (gel ­ 90s), aPDT ­ scaling and antimicrobial photodynamic therapy (toluidine blue O 100 µg/ml and red laser). Periodontal clinical parameters as probing depth (PD), clinical attachment level (CAL), recession height (RH), keratinized mucosa height (KMH), keratinized mucosa width (KMW), bleeding on probing (BOP), plaque index (PI) and percentage of root coverage (%RC) were evaluated by a blinded calibrated examiner at baseline, 3, 6, 12 months. Dentinal hyperestesia (HYPER) and patient esthetic perception (EST) were recorded in a visual analogic scale at baseline, 7 and 14 days, 1, 3, 6,and 12 months. Statistical analysis of CAL values was done with ANOVA complemented by Tukey. Friedman's complemented by Wilcoxon's test evaluated intragroup data, while Kruskal-Wallis was applied for intergroup data. Comparison for %RC was done by Kruskal-Wallis complemented by Dunnet (p<0.05). Reduction in CAL was observed in all groups, with higher numbers for CA (0.55±1.68) and aPDT (0.80±2.11) in relation to C (2.50±1.99) (p<0.05). A higher reduction in RH was observed for CA (0.40±0.62) compared to C (1.15±1.04) (p<0.05). There was an improvement of KMH and KMW for all groups, but with higher values of KMW for aPDT (2.80±0.733) and CA (2.33±0.60) in relation to C (1.99±0.65) (p<0.05). There were no differences for PD (p>0.05). There was a significant higher %RC for CA (82%±30%) and aPDT (82%±28%) in relation to C (58%±40%) (p<0.05). In general, BOP and PI were similar for all groups and periods (p>0.05). There was a significant reduction in hyperestesia along time, but for CA (1.20±2.23) and aPDT (0.70±1.15) the reduction was higher than C (2.63±2.22) (p<0.05). Esthetic perception was improved along time with higher values for CA (9.40±0.96) than C (9.40±0.96) (p<0.05). In conclusion, root biomodification agents as CA and aPDT favored recession coverage by STCG, resulting in reduction of dentin hypersensitivity, higher percentage of root coverage and more sites with total coverage. Additionally, these results had a long-term stability. (AU)


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Acid Etching, Dental/methods , Anti-Bacterial Agents/therapeutic use , Gingival Recession/surgery , Gingivoplasty/methods , Photochemotherapy/methods , Tetracycline/therapeutic use , Tooth Root/drug effects , Analysis of Variance , Dental Plaque Index , Gingiva/transplantation , Reproducibility of Results , Statistics, Nonparametric , Time Factors , Treatment Outcome
14.
Rev. ADM ; 73(6): 303-309, nov.-dic. 2016. ilus, tab
Article in Spanish | LILACS | ID: biblio-869341

ABSTRACT

La terapia fotodinámica es considerada como un procedimiento no invasivo dentro de la práctica periodontal, reduciendo la morbilidad e incrementando la comodidad del paciente. Objetivo: el objetivo del presente estudio fue evaluar la eficacia clínica del uso de terapia fotodinámica como adyuvante al raspado y alisado radicular en periodontitis crónica. Material y métodos: se realizó un estudio comparativo, correlacional, aplicado a 20 pacientes con periodontitis crónica moderada-avanzada (pérdida de inserción clínica >- 6 mm) durante el raspado y alisado radicular no quirúrgico, con la adición de azul de metileno, donde se administró la terapia fotodinámica en dos sesiones, evaluando los parámetros clínicos (profundidad de bolsa, nivel de inserción clínico, índice de placa, sangrado al sondeo y recesión gingival). Resultados: tras cuatro semanas de evaluación postratamiento, no se observaron diferencias significativas en los parámetros clínicos entre el grupo experimental y control, aunque se encontró un mayor aumento de recesión gingival con el uso de la terapia fotodinámica (p=0,353). Conclusión: el uso de la terapia fotodinámica como adyuvante durante el raspado y alisado radicular convencional no proporciona beneficios adicionales al tratamiento periodontal convencional.


Photodynamic therapy is considered a non-invasiveprocedure in periodontal practice, one which reduces morbidity andincreases patient comfort. Objective: The aim of this study was toevaluate the clinical effi cacy of photodynamic therapy as an adjuvantto scaling and root planing in chronic periodontitis. Material andmethods: A comparative and correlational study was carried out on 20patients with moderate to advanced chronic periodontitis (attachmentloss ≥ 6 mm) during nonsurgical scaling and root planing, including theaddition of methylene blue. The photodynamic therapy was applied intwo sessions, during which the clinical parameters (pocket depth, clinicalattachment level, plaque index, bleeding on probing, and gingivalrecession) were assessed. Results: After four weeks of post-treatmentassessment, no signifi cant diff erences were found between the clinicalparameters of the experimental and control groups, although a greaterincrease in gingival recession was found when photodynamic therapywas used (p = 0.353). Conclusion: The use of photodynamic therapyas an adjuvant in conventional scaling and root planing provides noadditional benefi ts to conventional periodontal treatmen.


Subject(s)
Humans , Male , Adult , Female , Middle Aged , Aged , Root Planing/methods , Photochemotherapy/methods , Chronic Periodontitis/therapy , Dental Scaling/methods , Analysis of Variance , Methylene Blue/therapeutic use , Periodontal Pocket/diagnosis , Periodontal Index , Statistical Analysis , Treatment Outcome
15.
Rev. Círc. Argent. Odontol ; 73(223): 4-9, oct. 2016. tab, ilus, graf
Article in Spanish | LILACS | ID: biblio-908062

ABSTRACT

El propósito de esta presentación es marcar el concepto de que la asociación entre enfermedad periodontal y diabetes es una vía bidireccional; la primera puede determinar el inicio o agravamiento de la diabetes, en tanto que ésta es uno de los factores de riesgo más estudiados que puede aumentar la gravedad de las enfermedades periodontales que son siempre iniciadas por los microorganismos patógenos.


Subject(s)
Male , Female , Humans , Diabetes Mellitus/pathology , Periodontal Diseases/etiology , Risk Factors , Anti-Bacterial Agents/therapeutic use , Dental Care for Chronically Ill/methods , Dental Plaque/prevention & control , Dental Plaque/therapy , Dental Scaling/methods , Diabetes Mellitus/drug therapy , Diabetes Mellitus/genetics , Periodontal Diseases/therapy , Photochemotherapy/methods
16.
Rev. Assoc. Paul. Cir. Dent ; 70(2): 126-130, abr.-jun. 2016. graf, ilus
Article in Portuguese | LILACS, BBO | ID: lil-797062

ABSTRACT

O presente estudo avaliou diferentes parâmetros como concentração do fotossensibilizador,tempo/energia de irradiação e uso de fibras ópticas, na a PDT para redução bacteriana intracanal.Avaliou-se, in vitro, em cubetas de quartzo contendo concentrações de 50, 100, 150 e 300μM de solução aquosa de Azul de Metileno (AM) a produção de espécies reativas de oxigênio (EROs) em irradiações adicionais de 30s com um laser de diodo emitindo em 660nm. Utilizando-se a mesma metodologia, foi avaliado os efeitos na produção de EROs com e sem o uso de fibra óptica. Concentrações de 50, 150 e 300μM de AM e análise por imagens, avaliaram a formação do fenômeno escudo óptico. Dez incisivos contaminados com P. Aeruginosas bioluminescentes, foram utilizados para analisar as energias/tempo para redução bacteriana intracanal. Imagens obtidas nos dentes avaliaram a contaminação inicial. Os canais foram preenchidos com o fotossensibilizador (PS) eirradiações de 2,4J foram realizadas. A cada irradiação, imagens foram obtidas e a redução bacteriana avaliada. A formação de EROs é maior em concentrações menores do PS, assim como a formação de escudo óptico. O uso de fibra aumenta a formação de ERO quando comparado a irradiação sem fibra. Irradiação com 7J impossibilitou a detecção de biofilme intracanal. A concentração do PS em que há maior eficiência na formação de EROs e menor formação de escudo óptico se encontra entre 50 a 100μM. O uso de fibras ópticas contribui para maior formação de EROs. Energia de irradiação mínima de 7J promove significativa redução bacteriana intracanal.


This study evaluates different approach such as photos sensitizer (PS) concentration, irradiation time/energy, and the use of optical fiber for intracanal microbial reduction. In a quartzcurvet, aqueous solution of Methylene blue at 50, 100, 150 and 300μM was tested for reactive oxygen species production (ROS) after successive irradiations of 30s with a diode laser (660nm,100mW). Using the same methodology, the ROS production was tested using an optical fiber. Image analyses evaluated the presence of optical shield in 3 different concentration of PS. Tencentral incisors were contaminated with bioluminescent P. aeruginosas to test the ideal energy/time for endodontic microbial reduction. Initial contamination was recorded by image after biofilm grown. The root canals were ful filled with Ps and irradiated with successive energiesof 2.4J. After every irradiation new images were recorded to compare the microbial reduction. ROS formation was improved using low concentration of PS, such as optical shield formation. The use of optical fiber did enhance the ROS formation when compared to irradiation with thelaser tip. Energy of 7J was the minimal energy to achieve lost of bioluminescent signal. For more efficient ROS production and minor optical shield presence, a concentration between 50 and 100μM seams to be ideal. The use of an optical fiber improves ROS production. Energy of 7J promotes significative intracanal de contamination.


Subject(s)
Humans , Male , Female , Endodontics/methods , Endodontics/standards , Endodontics , Photochemotherapy/adverse effects , Photochemotherapy/instrumentation , Photochemotherapy/methods , Lasers/adverse effects , Lasers , Microbiology/classification , Microbiology/statistics & numerical data
17.
An. bras. dermatol ; 91(2): 135-140, Mar.-Apr. 2016. tab, graf
Article in English | LILACS | ID: lil-781356

ABSTRACT

Abstract: BACKGROUND: Dermatophytes are filamentous keratinophilic fungi. Trichophyton rubrum is a prevalent infectious agent in tineas and other skin diseases. Drug therapy is considered to be limited in the treatment of such infections, mainly due to low accessibility of the drug to the tissue attacked and development of antifungal resistance in these microorganisms. In this context, Photodynamic Therapy is presented as an alternative. OBJECTIVE: Evaluate, in vitro, the photodynamic activity of four derivatives of Protoporphyrin IX by irradiation with LED 400 nm in T. rubrum. METHOD: Assays were subjected to irradiation by twelve cycles of ten minutes at five minute intervals. RESULT: Photodynamic action appeared as effective with total elimination of UFCs from the second irradiation cycle. CONCLUSION: Studies show that the photodynamic activity on Trichophyton rubrum relates to a suitable embodiment of the photosensitizer, which can be maximized by functionalization of peripheral groups of the porphyrinic ring.


Subject(s)
Photochemotherapy/methods , Protoporphyrins , Trichophyton/drug effects , Photosensitizing Agents/pharmacology , Reference Values , Time Factors , Tinea/drug therapy , Colony Count, Microbial , Reproducibility of Results , Analysis of Variance , Arthrodermataceae/drug effects , Antifungal Agents/pharmacology
18.
Braz. oral res. (Online) ; 30(1): e47, 2016. tab, graf
Article in English | LILACS | ID: biblio-951992

ABSTRACT

Abstract This study was conducted to assess the clinical effect of photodynamic therapy (PDT) in the decontamination of the deep dentin of deciduous molars submitted to partial removal of carious tissue. After cavity preparation, dentin samples were taken from the pulp wall of nineteen deciduous molars before and after PDT application. Remaining dentin was treated with 0.01% methylene blue dye followed by irradiation with an InGaAlP diode laser (λ - 660 nm; 40 mW; 120 J/cm2; 120 s). Dentin samples were microbiologically assessed for the enumeration of total microorganisms, Lactobacillus spp. and mutans streptococci. There was no significant difference in the number of colony-forming units (CFU) for any of the microorganisms assessed (p > 0.05). Photodynamic therapy, using 0.01% methylene blue dye at a dosimetry of 120 J/cm2 would not be a viable clinical alternative to reduce bacterial contamination in deep dentin.


Subject(s)
Humans , Male , Female , Child , Photochemotherapy/methods , Tooth, Deciduous/microbiology , Dental Caries/prevention & control , Dentin/drug effects , Enzyme Inhibitors/administration & dosage , Methylene Blue/administration & dosage , Streptococcus mutans/drug effects , Streptococcus mutans/radiation effects , Time Factors , Colony Count, Microbial , Treatment Outcome , Statistics, Nonparametric , Dentin/radiation effects , Dentin/microbiology , Lasers, Semiconductor/therapeutic use , Lactobacillus/drug effects , Lactobacillus/radiation effects
19.
São Paulo; s.n; s.n; 2016. 149 p. tab, graf, ilus.
Thesis in Portuguese | LILACS | ID: biblio-847515

ABSTRACT

O câncer de mama apresenta-se como um dos maiores problemas de saúde pública no mundo por ser, entre as mulheres, o primeiro em incidência e o segundo em número de óbitos. Este tipo de câncer apresenta alto grau de recorrência devido a falha no tratamento do tumor primário e o aparecimento de metástases da doença é a principal causa de mortalidade. As estratégias de tratamento nesse estágio não são específicas e comprometem a qualidade de vida das pacientes. A terapia fotodinâmica (PDT) aparece como uma alternativa terapêutica promissora. No entanto, a eficácia da PDT para o tratamento de tumores da mama, bem como os mecanismos que conduzem à morte celular permanecem obscuros. Para este propósito, neste estudo, nos propusemos a investigar mais profundamente os mecanismos moleculares envolvidos na morte celular induzida MB-PDT. Observou-se que a MB-PDT induz massiva morte celular em células tumorais preferencialmente. As células não-tumorigênicas foram significativamente mais resistentes à terapia em comparação com as células malignas. Análises morfológicas e bioquímicas das células em processo de morte apontou para mecanismos alternativos à apoptose clássica. A autofagia induzida por MB-PDT resultou em citoproteção ou citotoxicidade, dependendo do modelo de célula utilizado. No entanto, o comprometimento de uma destas vias não impediu o destino fatal das células tratadas com MB-PDT. Ao analisar os mecanismos de necrose regulada, observamos que a necroptose desempenhou um papel importante na morte celular induzida por MB-PDT. Adicionalmente, nossos resultados mostraram uma localização preferencial do MB nos lisossomos, o que nos levou a analisar o comprometimento destas organelas. Nós demonstramos por meio da atividade de catepsinas no citosol das células tratadas com MB-PDT, evidências de que a permeabilização da membrana lisossomal é outro mecanismo de necrose regulada responsável pelos efeitos citotóxicos da MB-PDT. Além de resistência à morte celular, as células tumorais também desenvolvem mecanismos de evasão do sistema imune. Neste estudo, também demonstramos que a MB-PDT tem o potencial de aumentar o número de células de linfócitos T CD4+. Por fim, também mostramos a eficácia da terapia em induzir morte celular seletiva às células tumorais em um modelo que recapitula a morfologia do epitélio glandular. Em resumo, demonstramos que vários mecanismos de morte celular são ativados por MB-PDT, uma vez que o comprometimento de uma via de morte celular não impediu o destino fatal das células tratadas com MB-PDT. No geral, os resultados obtidos nesta tese apontam a MB-PDT como uma terapia alternativa e eficaz para o tratamento do câncer de mama, exibindo uma ação de amplo espectro em células com diferentes mecanismos de resistência a vias de morte celular clássica, uma propriedade fundamental para uma terapia contra o câncer. Finalmente, as nossas observações destacam o potencial de MB-PDT como uma estratégia altamente eficaz para tratar o câncer de mama com segurança e possivelmente outros tipos de tumores


Breast cancer is the main cause of mortality among women presenting high recurrence due to primary treatment failure. Photodynamic therapy (PDT) appears as a promising therapeutic alternative. However, the efficacy of PDT to treat breast tumors as well as the mechanisms that lead to tumorigenic cell death remain unclear. For this purpose, in this study, we set out to deeper investigate the molecular mechanisms involved in MB-PDT induced cell death. We observed that MB-PDT differentially induces massive cell death of tumor cells. Non-malignant cells were significantly more resistant to the therapy compared to malignant cells. Morphological and biochemical analysis of dying cells pointed to alternative mechanisms rather than classical apoptosis. MB-PDT-induced autophagy resulted in either cytoprotection or cytotoxicity depending on the cell model used. However, impairment of one of these pathways did not prevent the fatal destination of MB-PDT treated cells. When analyzing regulated necrosis mechanisms, we observed that necroptosis played an important role in MB-PDT-induced cell death. Additionaly, our results have shown preferential lysosomal MB localization. Furthermore, we have also presented evidences that the lysosome membrane permeabilization is another regulated necrosis mechanism responsible for MB-PDT cytotoxic effects. It is known that in addition to resistance to tumor cell killing therapies, malignant cells develop molecular mechanisms in order to evade the immune system. In this study, we have also been able to show that the MB-PDT has the potential to increase the number of T lymphocytes CD4+. Additionally, when using a physiological 3D culture model that recapitulates relevant features of normal and tumor breast tissue morphology, we found that MB-PDT differential action in killing tumor cell was even higher than what was detected in 2D cultures. In summary, we demonstrated that different cell death mechanisms are being activated upon MB-PDT induction, since impairment of only one cell death pathway did not prevent the fatal destination of MB-PDT treated cells. Overall, our observations point MB-PDT as an alternative and effective therapy for breast cancer treatment, displaying a broad-spectrum action on tumors with different resistance mechanisms to classic cell death pathways, a desired property for improving an anticancer therapy. Finally, our observations underscore the potential of MB-PDT as a highly efficient strategy to safely treat breast cancer and possibly other types of tumors


Subject(s)
Breast Neoplasms/prevention & control , Cytotoxins/analysis , Photochemotherapy/methods , Cell Death , Methylene Blue/analysis
20.
An. bras. dermatol ; 90(6): 846-850, Nov.-Dec. 2015. tab, graf
Article in English | LILACS | ID: lil-769517

ABSTRACT

Abstract: BACKGROUND: Most available studies on the efficacy of topical photodynamic therapy focus on short-to medium-term results. Long-term data are scarce. OBJECTIVE: To evaluate the long-term efficacy of photodynamic therapy with topical methylaminolevulinate to treat Bowen's disease and basal cell carcinoma in the clinical practice setting of a dermato-oncology department. METHODS: The study included patients diagnosed with Bowen's disease or basal cell carcinoma, and who received photodynamic therapy from 2004 to 2008. Treatment protocol and clinical follow-up were standardized. The primary endpoint was clinically observed recurrence in a previous photodynamic therapy-treated area. Descriptive and survival analyses were performed. RESULTS: A total of 31 Bowen's disease lesions and 44 superficial basal cell carcinoma were treated, with a median follow-up of 43.5 months. Recurrence was observed in 14 Bowen's disease lesions (53.8%) and in 11 superficial basal cell carcinoma (33.3%). Significantly higher estimates for recurrence rates were found in patients with Bowen's disease (p=0.0036) or those aged under 58 years (p=0.039). The risk of recurrence was higher in patients with Bowen's disease than in those with superficial basal cell carcinoma and younger patients. CONCLUSIONS: Recurrence should be considered when choosing to treat non-melanoma skin cancer with photodynamic therapy. Younger age and Bowen's disease were independent predictors for long-term recurrence, suggesting the need to establish an extended period of follow-up for this subset of patients.


Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Aminolevulinic Acid/analogs & derivatives , Bowen's Disease/drug therapy , Carcinoma, Basal Cell/drug therapy , Neoplasm Recurrence, Local , Photochemotherapy/methods , Photosensitizing Agents/therapeutic use , Skin Neoplasms/drug therapy , Administration, Cutaneous , Age Factors , Aminolevulinic Acid/therapeutic use , Follow-Up Studies , Retrospective Studies , Risk Assessment , Statistics, Nonparametric , Time Factors , Treatment Outcome
SELECTION OF CITATIONS
SEARCH DETAIL