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1.
Arq. Inst. Biol. (Online) ; 89: e00022022, 2022. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1416790

ABSTRACT

The state of Piauí is in a Cerrado/Caatinga transition area, which is known the richness of its fauna and flora. Therefore, this work aims to know the diversity of fruit fly species in cashew orchards and native forest fragments in the south-central region of Piauí. Fruit flies were collected in McPhail traps and by sampling fruits. The material collected from the traps and the fruits in the two areas were taken to the Laboratory of Phytotechnics from Campus Prof. Cinobelina Elvas at the Universidade Federal do Piauí (UFPI/CPCE), municipality of Bom Jesus, Piauí, Brazil. In McPhail traps, six species of the genus Anastrepha: A. alveata, A. dissimilis, A. fraterculus, A. manihoti, A. obliqua, and A. zenildae were captured, of which A. fraterculus, A. obliqua, and A. zenildae were present in both areas. Anastrepha alveata, A. dissimilis and A. manihoti occurred only in the cashew orchard. In fruit sampling, A. fraterculus, A. obliqua, and A. zenildae infested wild-guava fruits, Myrcia tomentosa (Myrtaceae), in native forest. Anastrepha fraterculus, A. obliqua and A. zenildae were obtained directly from M. tomentosa fruits and from traps. The availability of host fruits is the factor that most influences the population fluctuation of fruit flies, mainly in the native forest area. Anastrepha manihoti is reported for the first time in the state of Piauí. This is the first record of A. fraterculus, A. obliqua and A. zenildae, infesting wildguava fruits, M. tomentosa.


Subject(s)
Tephritidae/classification , Psidium/parasitology , Biodiversity
2.
Pesqui. bras. odontopediatria clín. integr ; 22: e210080, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1386816

ABSTRACT

Abstract Objective: To assess the antibacterial activity of Psidium guajava fractions and their effects on adhesion of a multispecies biofilm consisting of Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis in vitro. Material and Methods: Guava leaves were obtained from the mountains of northern Peru, where they grow wild and free of pesticides. The antimicrobial activity of 25 mg/mL petroleum ether, 25 mg/mL dichloromethane and 25 mg/mL methanol fractions of P. guajava was evaluated by measuring inhibition halos, as well as the effect on the adhesion of multispecies biofilms at 4, 7 and 10 days of growth by measuring the optical density. In addition, antimicrobial susceptibility was compared using the Kruskal-Wallis test and its multiple comparison tests, and differences in mean biofilm adhesion between each fraction were assessed by repeated measures analysis and the Tukey multiple comparison test. Results: The rank-based Kruskal-Wallis test highlighted differences in the effects of the fractions on the zone of inhibition for each oral bacterium, including S. gordonii (p=0.000), F. nucleatum (p=0.000), and P. gingivalis (p=0.000), the Tukey test showed that the group treated with 0.12% chlorhexidine exhibited the least amount of adhesion, followed by the group treated with the 1.56 mg/mL methanol fraction. Conclusion: The methanol fraction of P. guajava had an antibacterial effect on S. gordonii and P. gingivalis, and the 1.56 mg/mL methanol fraction decreased biofilm adhesion.


Subject(s)
Periodontitis/microbiology , Biofilms , Psidium/chemistry , Streptococcus gordonii/pathogenicity , Anti-Bacterial Agents/pharmacology , Streptococcal Infections , In Vitro Techniques , Intervention Studies , Analysis of Variance
3.
Braz. j. biol ; 81(3): 728-736, July-Sept. 2021. tab, graf
Article in English | LILACS | ID: biblio-1153401

ABSTRACT

Abstract Xylella fastidiosa is a plant-pathogenic bacterium that lives inside host xylem vessels, where it forms biofilm which is believed to be responsible for disrupting the passage of water and nutrients. Pectobacterium carotovorum is a Gram-negative plant-specific bacterium that causes not only soft rot in various plant hosts, but also blackleg in potato by plant cell wall degradation. Chagas disease, which is caused by Trypanosoma cruzi, has been commonly treated with nifurtimox and benzonidazole, two drugs that cause several side effects. As a result, the use of natural products for treating bacterial and neglected diseases has increased in recent years and plants have become a promising alternative to developing new medicines. Therefore, this study aimed to determine, for the first time, the chemical composition of essential oil from Psidium guajava flowers (PG-EO) and to evaluate its in vitro anti-Xylella fastidiosa, anti-Pectobacterium carotovorum, anti-Trypanosoma cruzi and cytotoxic activities. PG-EO was obtained by hydrodistillation in a Clevenger apparatus while its chemical composition was determined by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Major compounds identified in PG-EO were α-cadinol (37.8%), β-caryophyllene (12.2%), nerolidol (9.1%), α-selinene (8.8%), β-selinene (7.4%) and caryophyllene oxide (7.2%). Results showed that the PG-EO had strong trypanocidal activity against the trypomastigote forms of Trypanosoma cruzi (IC50 = 14.6 μg/mL), promising antibacterial activity against X. fastidiosa (MIC = 12.5 μg/mL) and P. carotovorum (MIC = 62.5 μg/mL), and moderate cytotoxicity against LLCMK2 adherent epithelial cells in the concentration range (CC50 = 250.5 μg/mL). In short, the PG-EO can be considered a new source of bioactive compounds for the development of pesticides and trypanocide drugs.


Resumo Xylella fastidiosa é uma bactéria patogênica que vive dentro dos vasos do xilema hospedeiro, onde forma um biofilme responsável por interromper a passagem de água e nutrientes. Pectobacterium carotovorum é uma bactéria Gram-negativa que causa não só podridão macia em várias plantas hospedeiras, mas também canela-preta na batata por degradação da parede celular da planta. A doença de Chagas, causada pelo Trypanosoma cruzi, é comumente tratada com nifurtimox e benzonidazol, duas drogas que causam vários efeitos colaterais. Como resultado, o uso de produtos naturais para o tratamento de doenças bacterianas e negligenciadas aumentou nos últimos anos e as plantas continuam sendo uma alternativa promissora para o desenvolvimento de novos medicamentos. Portanto, este estudo teve como objetivo determinar, pela primeira vez, a composição química do óleo essencial de flores de Psidium guajava (PG-EO) e avaliar suas propriedades anti-Xylella fastidiosa, anti-Pectobacterium carotovorum, anti-Trypanosoma cruzi e citotóxica in vitro. PG-EO foi obtido por hidrodestilação em um aparelho Clevenger, enquanto sua composição química foi determinada por cromatografia em fase gasosa com detecção por ionização por chama (CG-DIC) e por cromatografia em fase gasosa acoplada à espectrometria de massa (CG-EM). Os principais compostos identificados no PG-EO foram α-cadinol (37,8%), β-cariofileno (12,2%), nerolidol (9,1%), α-selineno (8,8%), β-selineno (7,4%) e óxido de cariofileno (7,2%). Os resultados mostraram que o PG-EO apresentou forte atividade tripanocida contra as formas tripomastigotas de T. cruzi (CI50 = 14,6 μg/mL), promissora atividade antibacteriana contra X. fastidiosa (MIC = 12,5 μg/mL) e P. carotovorum (MIC = 62,5 μg/mL) e citotoxicidade moderada contra células epiteliais aderentes (LLCMK2) na faixa de concentração (CC50 = 250,5 μg/mL). Em suma, o PG-EO pode ser considerado uma nova fonte de compostos bioativos para o desenvolvimento de pesticidas e drogas tripanocidas.


Subject(s)
Oils, Volatile/pharmacology , Myrtaceae , Psidium , Microbial Sensitivity Tests , Plant Leaves , Flowers , Xylella
4.
Vitae (Medellín) ; 28(3): 1-7, 2021-08-11. Ilustraciones
Article in English | LILACS, COLNAL | ID: biblio-1363287

ABSTRACT

Background: the current research studies why it is effective using Anrederacordifolia, Psidium guajava, and Pogostemon cablin by the local community as a traditional medicine for diarrhea treatment caused by Escherichiacoli bacteria. Objectives: We compared the inhibitor effectiveness of three leaf extracts against Escherichia coli; we also identified the anti-bacterial substances contained in leaf extracts. Methods: We determined the bacterial test activity using the "agar diffusion" method and the thin layer chromatography (TLC) as qualitative analysis for determining the anti-bacterial substances contained in the extract. Results: The Pogostemon cablin leaf extract contained terpenoids, phenolic, and flavonoids compound as bacterial inhibitors, and the comparison showed that Pogostemon cablin leaf extract had the greatest bacterial inhibition power. Conclusion: The antibiotic substances found in the leaf extracts of Anredera cordifolia, Psidium guajava, and Pogostemon cablin can be used as traditional medicine. The breakthrough was evidenced by the ability to inhibit Escherichia coli bacteria. This research shows that traditional medicine has ancient knowledge used by this paper


Antecedentes: la presente investigación estudia la eficacia del uso de Anredera cordifolia, Psidium guajava y Pogostemoncablin por la comunidad local como medicina tradicional para el tratamiento de la diarrea causada por la bacteria Escherichia coli. Objetivos: Comparamos la eficacia inhibidora de los extractos de tres hojas contra Escherichia coli; también identificamos las sustancias antibacterianas contenidas en los extractos de hojas. Métodos: Determinamos la actividad de la prueba bacteriana mediante el método de "difusión en agar" y la cromatografía en capa fina (TLC) como análisis cualitativo para determinar las sustancias antibacterianas contenidas en el extracto. Resultados: el extracto de hoja de Pogostemoncablin contenía compuestos terpenoides, fenólicos y flavonoides como inhibidores bacterianos, y la comparación mostró que el extracto de hoja de Pogostemon cablin tenía el mayor poder de inhibición bacteriana. Conclusión: El contenido de sustancias antibióticas que se encuentran en el extracto de hoja de Anredera cordifolia, Psidium guajava y Pogostemoncablin puede utilizarse como medicina tradicional. Esto se evidencia por la capacidad de inhibir la bacteria Escherichiacoli. Esta investigación muestra que la medicina tradicional tiene un conocimiento antiguo utilizado por este artículo


Subject(s)
Humans , Anti-Bacterial Agents , Agar , Psidium , Escherichia coli , Pogostemon , Anti-Infective Agents
5.
Article in Chinese | WPRIM | ID: wpr-888112

ABSTRACT

Twenty-six compounds, including sixteen meroterpenoids(1-16), a triterpenoid(17), four terpenoid derivatives(18-21), and five aromatic compounds(22-26), were isolated from the leaves of Psidium guajava. Their structures were identified by spectroscopic analyses including NMR and MS. Compounds 21-26 were obtained from plants of Psidium for the first time. Based on the structure,(R)-2-ethylhexyl 2H-1,2,3-triazole-4-carboxylate(24 a), an α-glucosidase inhibitor recently isolated from Paramignya trimera, should be revised as compound 24. Meroterpenoids 1-16 were evaluated for their antitumor and antifungal activities. Meroterpenoids psiguajadial D(4), guapsidial A(5), 4,5-diepipsidial A(7), guadial A(14), and guadial B(15) showed cytotoxicities against five human tumor cell lines(HL-60, A-549, SMMC-7721, MCF-7, and SW-480), among which 5 was the most effective with an IC_(50) of 3.21-9.94 μmol·L~(-1).


Subject(s)
Antifungal Agents/pharmacology , Humans , Magnetic Resonance Spectroscopy , Plant Extracts , Plant Leaves , Psidium , Terpenes
6.
Cienc. tecnol. salud ; 7(3): 412-441, 26 de noviembre 2020. ^c27 cmilus
Article in Spanish | LILACS, LIGCSA, DIGIUSAC | ID: biblio-1141472

ABSTRACT

Las infecciones respiratorias constituyen una importante causa de morbilidad y mortalidad a nivel mundial, incrementándose su relevancia ante la reciente infección por SARS-CoV-2, causante de la pandemia de COVID-19. Las opciones terapéuticas para esta infección respiratoria son escasas y sin eficacia comprobada. El objetivo de esta revisión fue buscar la información sobre plantas con actividad antiviral o viricida publicada en los últimos 10 años, en las bases de datos de Google Scholar, Scopus y PubMed. La búsqueda priorizó aquellas especies disponibles en Guatemala, la cual se complementó con la búsqueda de moléculas con actividad antiviral para finalmente postular aquellas que puedan prevenir la infección o aminorar la patogénesis del SARS-CoV-2. Se detectaron más de 170 especies con actividad antiviral y se organizó la información por país o región y tipo de actividad antiviral contra virus específicos. De las especies de mayor disponibilidad en Guatemala se seleccionaron 20. La revisión culmina con 15 artículos que proponen plantas o moléculas con potencial actividad específica en el manejo de la pandemia por SARS-CoV-2. Se concluye que existen especies vegetales (Curcuma longa, Echinacea purpurea, Psidium guajava, Allium sativum, Salvia officinalis y Eucalyptus globulus) y fitocompuestos vegetales (hesperidina, rutina, diosmina, apiina, aloe-emodina, piperina, capsaicina, curcumina, oleuropeina, rhamnetina y gallato de epicatequina) que podrían contribuir al manejo de la enfermedad. Se insta a académicos y autoridades a poner más atención a estas opciones terapéuticas que nos ofrece la naturaleza y que podrían contribuir a aliviar el colapso de los sistemas de salud prevalentes.


Respiratory infections are an important cause of morbidity and mortality worldwide, increasing their relevance by the recent SARS-CoV-2 infection causing the COVID-19 pandemic. Therapeutic options for this respiratory infection are scarce and without proven effectiveness. The objective of this review was the search for information on plants with antiviral or viricidal activity published in the last 10 years in the Google Scholar, Scopus, and PubMed databases. The search prioritized those species available in Guatemala, was completed with the search of molecules with potential to prevent infection or reduce the activity of SARS-CoV-2 infection. More than 170 species with antiviral activity were detected and the information organized in surveys by country or region, activity against specific viruses and antiviral information on the 20 most commonly available species in the country. It is complemented with a summary of 15 articles that proposed plants or molecules with potential specific activity in the management of the SARS-CoV-2 pandemic. It is concluded there are plant species (Curcuma longa, Echinacea purpurea, Psidium guajava, Allium sativum, Salvia officinalis and Eucalyptus globulus) and phytocompounds isolated from these species (hesperidin, rutin, diosmin, apiine, aloe-emodin, piperine, capsaicin, curcumin, oleuropein and epicatechin gallate) that could contribute to the management of the disease. Academics and authorities are urged to pay more attention to these therapeutic options that nature offer to us and could contribute to alleviate the collapse of the prevailing health systems in the country.


Subject(s)
Humans , Plants, Medicinal/drug effects , Respiratory Tract Infections , Therapeutics , Catechin/therapeutic use , Echinacea , Curcumin/therapeutic use , Salvia officinalis , Psidium , Diosmin , Aloe , Eucalyptus , Betacoronavirus , Garlic , COVID-19 , Guatemala , Hesperidin
7.
Bol. latinoam. Caribe plantas med. aromát ; 19(1): 15-28, ene. 2020. tab, ilus
Article in English | LILACS | ID: biblio-1102865

ABSTRACT

The aim of this study was to investigate the chemical composition and acaricidal effect of two Psidium species essential oils and selected compounds on Tetranychus urticae. Essential oils from the leaves of Psidium laruotteanum and Psidium myrsinites were obtained through hydrodistillation, analyzed using CG-FID and CG-MS and evaluated for toxicity to T. urticae by fumigation and residual contact. The susceptibility of T. urticae to monoterpenes and sesquiterpenes was also investigated. The major constituents of the P. laruotteanum oil were (E)-nerolidol (9.6 ± 0.4%) and γ-terpinene (9.4 ± 0.6%) and the major constituents of the P. myrsinites oil were ß-caryophyllene (21.2 ± 0.9%) and α-humulene (10.3 ± 0.5%). Based on the LC50 estimates, no significant differences were found between the two oils regarding toxicity by fumigation or residual contact. ß-Caryophyllene and (E)-nerolidol had the highest level of toxicity, independently of the method investigated. The findings indicate that both oils and selected constituents, especially ß-caryophyllene and (E)-nerolidol, are promising as natural acaricidal agents that affect T. urticae through more than one mode of action.


Los aceites esenciales de las hojas de Psidium laruotteanum y Psidium myrsinites se obtuvieron por hidrodestilación, se analizaron por CG-FID y CG-MS, y se evaluaron sus toxicidades por fumigación y contacto residual contra Tetranychus urticae. Se investigó también la susceptibilidad del T. urticae a monoterpenos y sesquiterpenos. En el aceite esencial de P. laruotteanum, (E)-nerolidol (9.6 ± 0.4%) y γ-terpinene (9.4 ± 0.6%) se identificaron como constituyentes mayoritarios, mientras que en el aceite esencial de P. myrsinites, ß-caryophyllene (21.2 ± 0.9%) y α-humulene (10.3 ± 0.5%) se encontraron como mayoritarios. Con base en las CL50 estimadas, no se observaron diferencias significativas entre las toxicidades de los aceites por fumigación, y tampoco por contacto residual. ßCaryophyllene y (E)-nerolidol presentaron las mayores toxicidades, independientemente del método investigado. Estos resultados indican, que los dos aceites, así como los constituyentes seleccionados, entre los que se destacan ß-caryophyllene y (E)-nerolidol, son promisores agentes acaricidas naturales por actuar en el T. urticae por más de un mecanismo de acción.


Subject(s)
Animals , Oils, Volatile/pharmacology , Plant Leaves/chemistry , Tetranychidae/drug effects , Psidium/chemistry , Acaricides/pharmacology , Terpenes/analysis , Brazil , Oils, Volatile/chemistry , Pest Control , Fumigation , Acaricides/chemistry , Flame Ionization , Gas Chromatography-Mass Spectrometry
8.
Braz. arch. biol. technol ; 63: e20190196, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132166

ABSTRACT

Abstract Endodontic infections result from oral pathogenic bacteria which reach and infect dental pulp, as well as surrounding tissues, through cracks, unrepaired caries and failed caries restorations. This study aims to determine the chemical composition of essential oil from Psidium cattleianum leaves (PC-EO) and to assess its antibacterial activity against endodontic bacteria. Antibacterial activity of PC-EO was evaluated in terms of its minimum inhibitory concentration (MIC) values by the broth microdilution method on 96-well microplates. Bacteria Porphyromonas gingivalis (MIC = 20 µg/mL), Prevotella nigrescens (MIC = 62.5 µg/mL), Fusobacterium nucleatum (MIC = 12.5 µg/mL), Actinomyces naeslundii (MIC = 50 µg/mL), Bacteroides fragilis (MIC = 12.5 µg/mL), Aggregatibacter actinomycetemcomitans (MIC = 6.25 µg/mL) and Peptostreptococcus anaerobius (MIC = 62.5 µg/mL) were evaluated and compared to chlorhexidine dihydrochloride (CDH), the positive control. PC-EO was obtained by hydrodistillation with the use of a Clevenger-type apparatus whereas its chemical composition was analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Viridiflorol (17.9%), β-caryophyllene (11.8%), 1,8-cineole (10.8%) and β-selinene (8.6%) were the major constituents found in PC-EO, which exhibited high antibacterial activity against all endodontic pathogens under investigation. Therefore, PC-EO, a promising source of bioactive compounds, may provide therapeutic solutions for the field of endodontics.


Subject(s)
Oils, Volatile/pharmacology , Chlorhexidine/pharmacology , Psidium/chemistry , Anti-Bacterial Agents/pharmacology , Peptostreptococcus/drug effects , Bacteroides fragilis/drug effects , Actinomyces/drug effects , Microbial Sensitivity Tests , Fusobacterium nucleatum/drug effects , Aggregatibacter actinomycetemcomitans/drug effects , Porphyromonas gingivalis/drug effects , Prevotella nigrescens/drug effects , Gas Chromatography-Mass Spectrometry
9.
Article in English | LILACS, MTYCI | ID: biblio-1145981

ABSTRACT

La búsqueda de fuentes naturales para controlar los microorganismos es de interés en la producción de alimentos. Este estudio evaluó la composición química y la actividad antimicrobiana del aceite esencial de las hojas de Psidium cattleianum. El aceite esencial se extrajo por hidrodestilación y se identificó por GC-MS. La clase predominante de compuestos fueron los sesquiterpenos (47,6%) y los principales fueron trans-ß-cariofileno (14,7%), 1,8-cineol (11,7%) y É£-muuroleno (5,6%). Actividad antimicrobiana se realizó mediante la técnica de microdilución contra ocho hongos y ocho bacterias. Concentración inhibitoria mínima varió de 0,17 a 11,25 mg mL-1 para hongos y de 1,40 a 16,87 mg mL-1 para bacterias. Principales actividades fueron contra hongos Aspergillus fumigatus (ATCC 1022), Aspergillus ochraceus (ATCC 12066), Aspergillus versicolor (ATCC 11730) y Trichoderma viride (IAM 5061), y bacterias Pseudomonas aeruginosa (ATCC 27853), Bacillus cereus (clinical isolate) y Staphylococcus aureus (ATCC 6538) con potencial para prevenir enfermedades transmitidas por alimentos.


The search for natural sources to control microorganisms is of interest in food production. This study evaluated the chemical composition and antimicrobial activity of the essential oil from Psidium cattleianum leaves. The essential oil was extracted by hydrodistillation, and identified by GC-MS. The predominant class of compounds was sesquiterpenes (47.6%) and the major compounds were trans-ß-caryophyllene (14.7%), 1,8-cineole (11.7%) and É£-muurolene (5.6%). The antimicrobial activity was carried out by microdillution technique against eight fungi and eight bacteria. The minimum inhibitory concentration ranged from 0.17 to 11.25 mg mL-1 for fungi, and from 1.40 to 16.87 mg mL-1 for bacteria. The highest activities were against fungi Aspergillus fumigatus (ATCC 1022), Aspergillus ochraceus (ATCC 12066), Aspergillus versicolor (ATCC 11730), and Trichoderma viride (IAM 5061), and bacteria Pseudomonas aeruginosa (ATCC 27853), Bacillus cereus (clinical isolate), and Staphylococcus aureus (ATCC 6538) with potential to prevent foodborne diseases.


Subject(s)
Psidium/chemistry , Anti-Infective Agents , Aspergillus , Aspergillus fumigatus , Trichoderma , Aspergillus ochraceus , Plant Extracts
10.
Braz. j. biol ; 79(4): 697-702, Nov. 2019. tab
Article in English | LILACS | ID: biblio-1001477

ABSTRACT

Abstract This study evaluated the antibacterial and antiproliferative activities of the essential oil of Psidium guajava leaves (PG-EO), traditionally used in folk medicine. The essential oil was obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. The major PG-EO chemical constituents were identified by GC-MS and GC-FID as being β-caryophyllene (16.1%), α-humulene (11.9%), aromadendrene oxide (14.7%), δ-selinene (13.6%), and selin-11-en-4α-ol (12.5%). The antibacterial activity of the essential oil of P. guajava leaves was determined in terms of its minimum inhibitory concentrations (MIC) using the broth microdilution method in 96-well microplates. PG-EO had moderate activity against Streptococcus mutans (MIC = 200 µg/mL), S. mitis (MIC = 200 µg/mL), S. sanguinis (MIC = 400 µg/mL), S. sobrinus (MIC = 100 µg/mL), and S. salivarius (MIC = 200 µg/mL). The antiproliferative activity was evaluated against different tumor cell lines: breast adenocarcinoma (MCF-7), human cervical adenocarcinoma (HeLa), and human gliobastoma (M059J). A normal human cell line (GM07492A, lung fibroblasts) was included. The antiproliferative activity was evaluated using the XTT assay and the results were expressed as IC50. The essential oil showed significantly lower IC50 values against MCF-7 and M059J lines than that obtained for the normal line, showing selectivity. Our results suggest that the essential oil of Psidium guajava L. has promising biological activities and can be considered a new source of bioactive compounds.


Resumo Este estudo avaliou as atividades antibacteriana e antiproliferativa do óleo essencial das folhas frescas de Psidium guajava (PG-OE), tradicionalmente utilizadas na medicina popular. O óleo essencial foi obtido por hidrodestilação das folhas frescas, utilizando aparelho do tipo Clevenger. Os principais constituintes químicos de PG-OE identificados por CG-EM e CG-DIC foram: β-cariofileno (16,1%), α-humuleno (11,9%), óxido de aromadendreno (14,7%), δ-selineno (13,6%) e selin-11-en-4α-ol (12,5%). A atividade antibacteriana do óleo essencial das folhas de P. guajava foi determinada em termo de sua concentração inibitória mínima (CIM) utilizando o método de microdiluição de caldo em microplacas de 96 poços. PG-OE apresentou moderada atividade contra Streptococcus mutans (CIM = 200 μg/mL), S. mitis (CIM = 200 μg/mL), S. sanguinis (CIM = 400 μg/mL), S. sobrinus (CIM = 100 μg/mL) e S. salivarius (CIM = 200 μg/mL). A atividade antiproliferativa foi avaliada frente a diferentes linhagens de células tumorais como: adenocarcinoma de mama (MCF-7), adenocarcinoma cervical humano (HeLa) e gliobastoma humano (M059J). Foi incluída uma linhagem celular humana normal (GM07492A, fibroblastos pulmonares). A atividade antiproliferativa foi avaliada utilizando o ensaio XTT e os resultados foram expressos como CI50. As linhagens MCF-7 e M059J mostraram valores significativamente mais baixos de CI50 do que os obtidos para a linhagem normal, mostrando seletividade. Nossos resultados sugerem que o óleo essencial das folhas frescas de Psidium guajava L. possui atividades biológicas promissoras e pode ser considerado como uma nova fonte de compostos bioativos.


Subject(s)
Humans , Plant Leaves , Psidium , Cell Proliferation/drug effects , Phytochemicals/pharmacology , Anti-Bacterial Agents/pharmacology , Sesquiterpenes/pharmacology , Oils, Volatile/pharmacology , Plant Extracts/pharmacology , Microbial Sensitivity Tests , Azulenes/pharmacology , Monocyclic Sesquiterpenes , Polycyclic Sesquiterpenes , Gas Chromatography-Mass Spectrometry
11.
Biosci. j. (Online) ; 35(4): 1245-1255, july/aug. 2019. tab, ilus
Article in English | LILACS | ID: biblio-1048928

ABSTRACT

This study aimed to evaluate how the surrounding vegetation affects the assemblage of fruit flies. For this, flies were collected in guava orchards with distinct types of surrounding vegetation (exotic pasture, native forest and fruit tree intercropping). The experiment was conducted in Itaporã and Dourados, State of Mato Grosso do Sul, Brazil. Weekly samples were collected from March to April 2015. McPhail traps were baited with 5% hydrolyzed corn protein. Species richness, diversity index and abundance were evaluated for each orchard, as well as the species composition of fruit fly between the environments surrounding the orchard and, finally, the sex ratio. A total of 579 individuals were collected of the species Anastrepha fraterculus, A. montei, A. obliqua, A. sororcula, A. striata, A. turpiniae and A. zenildae. The orchard surrounded by native forest had the highest species richness and Shannon diversity. The species composition differed between environments, with the formation of three distinct groups. The environment surrounding the guava orchard influences the richness, diversity, abundance and sex ratio of fruit flies, indicating that the native forest may provide more resources for the maintenance of these insects.


Este estudo teve como objetivo avaliar como a vegetação do entorno dos pomares afeta a assembleia de moscas-das-frutas. Para isto, foram coletadas moscas em pomares de goiabeiras com distintos tipos de vegetação no entorno (pastagens exóticas, mata nativa e fruticultura). O experimento foi conduzido em Itaporã e Dourados, Estado do Mato Grosso do Sul, Brasil. Amostras semanais foram coletadas de março a abril de 2015. As armadilhas McPhail foram iscadas com 5% de proteína hidrolisada de milho. A riqueza de espécies, o índice de diversidade e a abundância foram avaliados para cada pomar, assim como a composição de espécies de moscas-das-frutas entre os ambientes do entorno do pomar e razão sexual. Um total de 579 indivíduos foram coletados das espécies Anastrepha fraterculus, A. montei, A. obliqua, A. sororcula, A. striata, A. turpiniae e A. zenildae. O pomar cercado por mata nativa apresentou maior riqueza de espécies e diversidade de Shannon. A composição das espécies diferiu entre os ambientes, com a formação de três grupos distintos. O ambiente do entorno do pomar de goiabeira influencia a riqueza, diversidade, abundância e razão sexual das moscas-das-frutas, indicando que a mata nativa pode fornecer mais recursos para a manutenção desses insetos.


Subject(s)
Ecosystem , Tephritidae , Psidium , Drosophila
12.
Biosci. j. (Online) ; 35(3): 837-848, may./jun. 2019. graf, tab
Article in English | LILACS | ID: biblio-1048690

ABSTRACT

Using nitrogen (N) to increase plant tolerance to salinity has been tested in many species. However, in addition to controversial results, most studies are conducted with annual species and/or in the initial growth stage and there are almost no studies with perennial fruit crops in the production stage. Thus, this study aimed to evaluate the production components and post-harvest quality of 'Paluma' guava irrigated with water of increasing salinity in soil fertilized with N. The experiment was carried out for two years in drainage lysimeters in an experimental area of the Federal University of Campina Grande (UFCG), Pombal, PB, Brazil. The experimental design was in randomized blocks, in 5 x 4 factorial scheme, with of five levels of water salinity ­ ECw (NaCl)(0.3, 1.1, 1.9, 2.7 and 3.5 dS m-1) and four N doses (ureia) (70, 100, 130 and 160% of the recommended dose - 541.1 mg of N dm-3 of soil per year), with three replicates. Number of fruits, mean fruit weight, production per plant, polar and equatorial diameters of fruit and contents of soluble solids and ascorbic acid (vitamin C) decreased linearly with the increase in irrigation water salinity from 0.3 dS m-1. The interaction between water salinity and N doses and the isolated action of N did not interfere statistically with the studied variables.


O uso de nitrogênio como forma de aumentar a tolerância das plantas à salinidade tem sido testado em muitas espécies. No entanto, além de resultados controversos, se observa que a maioria dos estudos são realizados com espécies anuais e, ou na fase inicial de crescimento, sendo praticamente inexistentes estudos com frutíferas perenes em fase de produção. Nessa direção, o trabalho teve como objetivo avaliar os componentes de produção e a qualidade pós-colheita de goiabeira 'Paluma' irrigada com águas de salinidade crescente no solo com nitrogênio. O experimento foi conduzido durante dois anos em lisímetros de drenagem numa área experimental da Universidade Federal de Campina Grande (UFCG), Pombal, PB. O delineamento experimental foi em blocos casualizados, em esquema fatorial 5 x 4, com salinidades de água ­ CEa (NaCl) de 0,3, 1,1, 1,9, 2,7 e 3,5 dS m-1 e quatro doses de nitrogênio (ureia) de 70, 100, 130 e 160% da dose recomendada de 541,1 mg de N dm-3 por ano, com três repetições. A interação salinidade da água x doses de nitrogênio e ação isolada de N não influenciaram estatisticamente as variáveis estudadas. O número de frutos, a massa média de frutos, a produção por planta, o diâmetro polar e equatorial dos frutos, os teores de sólidos solúveis e ácido ascórbico (vitamina C), decresceram linearmente com o aumento da salinidade da água de irrigação a partir de 0,3 dS m-1.


Subject(s)
Saline Waters , Saltpetre Soils , Psidium , Agricultural Irrigation , Nitrogen
13.
Biosci. j. (Online) ; 35(2): 377-388, mar./apr. 2019. ilus, tab
Article in English | LILACS | ID: biblio-1048592

ABSTRACT

Psidium guajava L. (guava) is an important species that presents high genetic variability due to its mixed reproductive system, which is desired in breeding programs. Repeatability is an important tool for the selection of genotypes in pre-breeding studies. When genetic variability is present, the knowledge regarding the number of samples to be used in repeatability studies is indispensable. This study aims to determine the number of necessary measures while optimizing resources and maintaining the reliability of the results for the variables evaluated in P. guajava. The experiment was carried out with genotypes from three Brazilian States: Espírito Santo, São Paulo, and Minas Gerais, and a total of 79 P. guajava genotypes were collected. The following characteristics were evaluated: young leaf length and width; developed leaf length and width; fruit length; fruit diameter and fruit cavity diameter; and fruit weight and pulp weight. For the evaluated characteristics, deviance, permanent phenotypic and temporary environment variance, coefficients of repeatability and determination, accuracy and the number of estimated measurements required were determined. We established that the number of measurements required in repeatability analysis for a coefficient of repeatability with a reliability of 80% is four, for the measurements of developed leaf width, pulp weight, fruit diameter, and fruit cavity diameter


Psidium guajava L. (goiaba) é uma espécie importante que apresenta alta variabilidade genética devido ao seu sistema reprodutivo misto, o que é desejado em programas de melhoramento. A repetibilidade é uma ferramenta importante para a seleção de genótipos em estudos de pré-melhoramento.Quando a variabilidade genética está presente, o conhecimento sobre o número de amostras a serem usadas em estudos de repetibilidade é indispensável. Este estudo tem como objetivo determinar o número de medidas necessárias, otimizando recursos e mantendo a confiabilidade dos resultados para as variáveis avaliadas em P. guajava. O experimento foi conduzido com genótipos de três estados brasileiros: Espírito Santo, São Paulo e Minas Gerais, e um total de 79 genótipos de P. guajava foram coletados. As seguintes características foram avaliadas: comprimento e largura das folhas jovens; comprimento e largura das folhas desenvolvidas; comprimento do fruto; diâmetro do fruto e diâmetro da cavidade do fruto; e peso do fruto e peso da polpa. Para as características avaliadas, foram determinados os desvios, a variância fenotípica permanente e temporária do ambiente, os coeficientes de repetibilidade e determinação, a precisão e o número estimado de medidas necessárias. Foi estabelecido que o número de medições necessárias na análise de repetibilidade para um coeficiente de repetibilidade com uma confiabilidade de 80% é igual a quatro, para as medidas de largura de folha desenvolvida, peso da polpa, diâmetro do fruto e diâmetro da cavidade do fruto.


Subject(s)
Biometry , Plant Leaves , Psidium , Plant Breeding
14.
Arq. Inst. Biol ; 86: e0242018, 2019. tab
Article in English | LILACS, VETINDEX | ID: biblio-1024604

ABSTRACT

Some species of Lonchaeidae (Diptera) are considered frugivorous and polyphagous pests, and are widely distributed in Neotropical regions. The relationship between a fly and its host plant is important for studies on behavior and distribution of frugivorous flies. The objective of this work was to identify the Lonchaeidae fly species and their host plants in the Cerrado biome, specifically in the state of Piauí, Brazil. Eighty-one adults (33 ♀ and 48 ♂) from the genus Neosilba McAlpine (Lonchaeidae), represented by the species Neosilba inesperata Strikis & Prado, Neosilba pendula Bezzi, and Neosilba zadolicha McAlpine, were collected from fruit samples. Oranges [Citrus sinensis (L.) Osbeck] and guava (Psidium guajava L.) had the highest frequencies of infestation. A Neosilba species was considered a primary invader in orange. These results are the first ones reported of Lonchaeidae species and their host plants in the state of Piauí.(AU)


Algumas espécies de Lonchaeidae (Diptera) são consideradas pragas frugívoras e polífagas, com ampla distribuição geográfica em regiões neotropicais. A relação mosca/planta hospedeira é de grande valor para estudos de comportamento e distribuição de moscas frugívoras. O objetivo deste trabalho foi identificar as espécies de Lonchaeidae e suas plantas hospedeiras no bioma Cerrado, especificamente no estado do Piauí, Brasil. Oitenta e um adultos (33 ♀ e 48 ♂) do gênero Neosilba McAlpine (Lonchaeidae), pertencentes às espécies Neosilba inesperata Strikis & Prado, Neosilba pendula Bezzi e Neosilba zadolicha McAlpine, foram coletados de amostras de frutas, sendo a laranja [Citrus sinensis (L.) Osbeck] e a goiaba (Psidium guajava L.) as de maiores frequências de infestação. Uma espécie de Neosilba foi considerada um invasor primário em laranjas. Esses resultados são os primeiros relatos de espécies de Lonchaeidae e suas plantas hospedeiras no estado do Piauí.(AU)


Subject(s)
Citrus , Psidium , Drosophila , Grassland , Diptera , Fruit
15.
Arq. Inst. Biol ; 86: e0682018, 2019. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1046002

ABSTRACT

Fruit flies are the biggest obstacle in guava cultivation, with the monitoring of population a fundamental aspect for their management. The objective of this study was to assess the effectiveness of attractive lures for fruit flies in guava trees, produced in an organic system. McPhail traps were installed with 400 mL of solution, in four repetitions, and distributed in randomized blocks in six treatments: Isca Mosca® (5%); Isca Samaritá Tradicional® (5%); Torula® yeast; Bio Anastrepha® (5%); Ceratrap® and guava juice (50%), with 3 weekly reviews. Data on capture were subjected to analysis of variance, and averages were compared (Tukey 5%). The values of fly trap per day (FTD) were computed. The correlation between flies and meteorological variables were evaluated, as well as the monthly cost of lures. A total of 37,917 individuals from the genus Anastrepha and 122 species of flies Ceratitis capitata were collected, being Anastrepha spp. the main genus of fruit fly in the region. The Ceratrap product proved to be superior, followed by Torula, Isca Mosca, and Bio Anastrepha; Isca Samaritá and guava juice presented the lowest results. The standard trapping of females was like the total. The uniformity of attraction presented a drop in all treatments, and Ceratrap was more constant after seven days. The correlations between meteorological factors and population variation were positive and significant only for precipitation. The most expensive treatments (Ceratrap and Torula) were also the most efficient and selective, and the Ceratrap product required less manpower.(AU)


A presença de mosca-das-frutas é o maior obstáculo à produção de goiabas, sendo o monitoramento populacional de fundamental importância para seu manejo. O objetivo deste trabalho foi avaliar a eficácia de iscas atrativas para mosca-das-frutas em goiabeiras, produzidas em um sistema orgânico. Foram instaladas armadilhas McPhail contendo 400 mL de solução, em quatro repetições e distribuição em blocos randomizados, em seis tratamentos: isca Mosca® (5%); isca Samaritá Tradicional® (5%); levedura Torula®; Bio Anastrepha® (5%); Ceratrap® e suco de goiaba (50%), com 3 avaliações semanais. Os dados da captura foram submetidos à análise de variância e às médias comparadas (Tukey 5%). Os valores de Mosca por Armadilha por Dia (MAD) foram calculados. A correlação entre moscas e as variáveis meteorológicas foi avaliada e o custo mensal das iscas foi calculado. Foram coletados 37.917 indivíduos do gênero Anastrepha e 122 moscas da espécie Ceratitis capitata, sendo a Anastrepha spp. o principal gênero de mosca-das-frutas da região. O produto Ceratrap mostrou-se superior, seguido pelas iscas Torula, Isca Mosca e Bio Anastrepha; a Isca Samaritá e o suco de goiaba apresentaram os menores resultados de captura. O padrão de captura de fêmeas foi semelhante ao total. Verificou-se uma queda na uniformidade de atração de todos os tratamentos, sendo o Ceratrap o que apresentou maior constância após sete dias. As correlações entre os fatores meteorológicos e a flutuação populacional foram positivas e significativas apenas para a precipitação. Os tratamentos mais onerosos (Ceratrap e Torula) também foram os mais eficientes e seletivos, sendo o produto Ceratrap o que demandou menor mão de obra.(AU)


Subject(s)
Tephritidae , Psidium , Pest Control , Meteorological Concepts
16.
Article in Chinese | WPRIM | ID: wpr-771671

ABSTRACT

The present study is to study the chemical constituents from ethanol extract of Psidium guajava leaves. The constituents were separated and purified by silica gel column chromaiographios over, macroporous resin D-101, Sephadex LH-20, and ODS. Six flavonoids compounds were isolated and identified as quercetin(1), quercetin-3---D-arabinopyranoside(2), quercetin-3---D-ribopyranoside(3), quercetin-3---D-galactopyranoside(4), quercetin-3---D-glucopyranoside(5), and quercetin-3--D-xylpyranoside(6). The antioxidant effects of six flavonoids was evaluated by scavenging ability of DPPH, superoxide anion, ABTS·⁺, and reducing effect of Fe³⁺ as well as total antioxidant capacity(FRAP). Vitamin C was used as positive control. The results indicated that six flavonoids exhibited significant antioxidant effects.


Subject(s)
Antioxidants , Chemistry , Flavonoids , Chemistry , Phytochemicals , Plant Leaves , Chemistry , Psidium , Chemistry , Quercetin , Chemistry
17.
Journal of Integrative Medicine ; (12): 350-357, 2018.
Article in English | WPRIM | ID: wpr-691053

ABSTRACT

<p><b>OBJECTIVE</b>This study examined the antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava against methicillin-resistant Staphylococcus aureus (MRSA) and used a standardized purification protocol to determine the presence and abundance of bioactive compounds in the leaf extracts.</p><p><b>METHODS</b>In vitro antimicrobial activities of the ethanolic extracts of C. sativa, T. orientalis and P. guajava were tested against MRSA. The presence of bioactive molecules in these three leaves was evaluated using biochemical assays and high-performance thin-layer chromatography (HPTLC).</p><p><b>RESULTS</b>Resistance to methicillin, penicillin, oxacillin and cefoxitin was observed in each of the clinical and nonclinical MRSA isolates. However, they were still vulnerable to vancomycin. Used individually, the 50% extract of each plant leaf inhibited MRSA growth. A profound synergism was observed when C. sativa was used in combination with T. orientalis (1:1) and when P. guajava was used in combination with T. orientalis (1:1). This was shown by larger zones of inhibition. This synergism was probably due to the combined inhibitory effect of phenolics present in the leaf extracts (i.e., quercetin and gallic acid) and catechin, as detected by HPTLC.</p><p><b>CONCLUSION</b>The leaf extracts of C. sativa, T. orientalis and P. guajava had potential for the control of both hospital- and community-acquired MRSA. Moreover, the inhibitory effect was enhanced when extracts were used in combination.</p>


Subject(s)
Anti-Bacterial Agents , Pharmacology , Cannabis , Drug Resistance , Humans , Methicillin , Pharmacology , Methicillin-Resistant Staphylococcus aureus , Microbial Sensitivity Tests , Phytotherapy , Plant Extracts , Pharmacology , Plant Leaves , Psidium , Staphylococcal Infections , Drug Therapy , Microbiology , Thuja
18.
Biosci. j. (Online) ; 33(5): 1305-1313, sept./oct. 2017. tab
Article in English | LILACS | ID: biblio-966337

ABSTRACT

Psidium myrsinites DC. is species known as "araçá", from the Cerrado (savanna) biome in Brazil. It is commonly used as a medicinal plant in the treatment of diarrhea because of its astringent properties. The aims of this study were to carry out phytochemical screening with an exploratory purpose; to investigate toxicity by brine shrimp (Artemia salina) lethality bioassay; and to evaluate antimicrobial activity against Gram-positive and Gram-negative bacteria by determining the minimum inhibitory concentration of the essential oil, acetonic and ethanolic crude extracts, and the fractions obtained with organic solvents of Psidium myrsinites DC. leaves. The phytochemical profile determined the major classes of secondary metabolites present as phenolic compounds (tannins, coumarins, flavonoids, anthraquinone glycosides and terpene compounds). The essential oil and hexane fraction demonstrated a level of strong and mild toxicity respectively, thus encouraging further research with isolated substances from them. The crude ethanolic and acetonic extracts, and hexane fractions, chloroform intermediary, ethyl acetate and aqueous fraction from crude acetonic extract showed the best inhibitory effect on bacterial growth of Gram-positive bacteria with minimum inhibitory concentrations ranging between 62.5 and 250 µg.mL-1. However, the presence of secondary compounds such as tannins, flavonoids and terpenes is probably associated with the inhibitory effect on tested microorganisms, which could justify the medicinal use of the leaves of this species.


Psidium myrsinites DC. é uma espécie do bioma Cerrado conhecida como "araçá" comumente utilizada como planta medicinal no tratamento de diarreias e na cicatrização devido as suas propriedades adstringentes. O objetivo deste trabalho foi realizar a triagem fitoquímica com propósito exploratório e a investigação da toxicidade pelo teste de letalidade em Artemia salina e da atividade antimicrobiana contra bactérias Gram-positivas e Gram-negativas pela determinação da concentração mínima inibitória do óleo essencial, dos extratos brutos acetônico e etanólico, além das frações obtidas com solventes orgânicos de polaridade crescente: hexano, clorofórmio, acetato de etila das folhas de Psidium myrsinites DC. O perfil fitoquímico determinou as principais classes de metabólitos secundários presentes como compostos fenólicos (taninos, cumarinas, flavonoides, heterosídeos antraquinônicos e compostos terpênicos. O óleo essencial e a fração hexano demonstraram nível de toxicidade forte e moderado respectivamente, incentivando assim novas pesquisas com substâncias isoladas dos mesmos. Os extratos brutos etanólico e acetônico, e as frações hexano, intermediária de clorofórmio, acetato de etila e fração aquosa do extrato bruto acetônico demonstraram melhor ação inibitória sobre o crescimento bacteriano de bactérias Gram-positivas com concentrações mínimas inibitórias variando entre 62,5 e 250 µg.mL-1. Contudo, a presença de compostos secundários tais como, taninos, flavonoides e terpenos, provavelmente, está associada ao efeito inibitório sobre os micro-organismos testados, o que poderia justificar o uso medicinal das folhas dessa espécie.


Subject(s)
Artemia , Tannins , Oils, Volatile , Psidium/toxicity
19.
Braz. dent. j ; 28(3): 372-379, May-June 2017. tab, graf
Article in English | LILACS | ID: biblio-888654

ABSTRACT

Abstract Psidium cattleianum (PC) has been displaying inhibitory effect against a variety of microorganisms, but this effect has not yet been tested against endodontic pathogens. The aim of this study was to evaluate the antimicrobial activity and biocompatibility of the aqueous (PCAE) and hydroethanolic (PCHE) extracts from Psidium cattleianum (PC) leaves. Minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) were determined using the microdilution broth method in order to analyze the antimicrobial effect against Enterococcus faecalis, Pseudomonas aeruginosa, Actinomyces israelii and Candida albicans in planktonic conditions. Biofilm assays were conducted only with the extracts that were able to determine the MLC for microorganisms in planktonic conditions. Immediate and late tissue reactions against PC extracts were evaluated using edemogenic test and histological analysis of subcutaneous implants in Wistar rats. The results showed that the MIC and MLC values ranged between 0.25 and 4 mg/mL. The MLC obtained for PCHE inhibited 100% growth of all the tested strains, except for C. albicans. PCAE had the same effect for E. faecalis and P. aeruginosa. Both PC extracts were able to eliminate E. faecalis biofilms and only the PCHE eliminated P. aeruginosa biofilms. The positive controls inhibited the growth of all tested strains in MIC and MLC essays, but no CHX tested concentrations were able to eliminate A. israelii biofilm. PCAE caused a discrete increase in the edema over time, while PCHE caused a higher initial edema, which decreased progressively. Both PCAE and PCHE extracts were biocompatible, but PCHE showed better results with slight levels of inflammation at 28 days. In conclusion, PCHE was biocompatible and presented better antimicrobial effect against important pathogens associated with persistent endodontic infections


Resumo Psidium cattleianum (PC) tem apresentado atividade inibitória frente diversos microrganismos, entretanto esse efeito ainda não foi testado contra microrganismos de interesse endodôntico. O objetivo desse estudo foi avaliar a atividade antimicrobiana e a biocompatibilidade dos extratos aquoso (EAPC) e hidroetanólico (EHPC) das folhas de Psidium cattleianum. As concentrações inibitória mínima (CIM) e letal mínima (CLM) foram determinadas pelo método de microdiluição em caldo, com o objetivo de analisar o efeito antimicrobiano frente Enterococcus faecalis, Pseudomonas aeruginosa, Actinomyces israelii e Candida albicans em condições planctônicas. Os ensaios de biofilme foram realizados somente com os extratos em que se determinou a CLM frente os microrganismos em condições planctônicas. Respostas teciduais imediata e tardia frente aos extratos de Psidium cattleianum foram avaliadas por teste edemogênico e análise histológica de implantes subcutâneos em ratos Wistar. Os resultados mostraram que CIM e CLM variaram entre 0,25 e 4 mg/mL. As CLMs determinadas pelo EHPC inibiram 100% do crescimento de todas as cepas testadas, exceto Candida albicans. EAPC apresentou o mesmo efeito para E. faecalis e P. aeruginosa. Ambos os extratos de PC conseguiram eliminar o biofilme de E. faecalis, e somente o EHPC eliminou o biofilme de P. aeruginosa. Os controles positivos inibiram o crescimento de todos os microrganismos testados nos ensaios de CIM e CLM, mas nenhuma das concentrações de clorexidina testadas foi capaz de eliminar o biofilme de A. israelii. O EAPC provocou um discreto aumento de edema com o tempo, enquanto EHPC provocou um edema inicial severo, que diminuiu progressivamente. Ambos os extratos EAPC e EHPC foram biocompatíveis, entretanto, EHPC apresentou melhores resultados com baixos níveis de inflamação em 28 dias. Pode-se concluir que EHPC foi biocompatível e apresentou melhor efeito antimicrobiano frente importantes patógenos associados a infecções endodônticas persistentes.


Subject(s)
Animals , Male , Anti-Infective Agents/pharmacology , Biocompatible Materials , Plant Extracts/pharmacology , Psidium/chemistry , Root Canal Therapy , Actinobacteria/drug effects , Biofilms , Candida albicans/drug effects , Enterococcus faecalis/drug effects , Microbial Sensitivity Tests , Pseudomonas aeruginosa/drug effects , Rats, Wistar
20.
Rev. cuba. plantas med ; 22(1)ene.-mar. 2017. tab
Article in Spanish | LILACS, CUMED | ID: biblio-901500

ABSTRACT

Introducción: el tamizaje fitoquímico de una planta brinda una percepción sobre los posibles beneficios medicinales; la literatura refiere información de esta determinación en las hojas deAnacardium occidentale L., Psidium guajava L., Morinda citrifolia L. y Moringa oleifera Lam. , no obstante, en cuanto al polvo mixto de sus hojas no se encontró información disponible. Objetivo: determinar los metabolitos secundarios mediante tamizaje fitoquímico a los polvos mixtos de hojas de A. occidentale, P. guajava, M. citrifolia y M. oleifera. Métodos: se recolectaron las hojas de las plantas en estudio, estas se deshidrataron a temperatura ambiente y artificial, seguidamente se trituraron a 1 mm de tamaño de partículas. Para la elaboración de las muestras se tomó el polvo seco obtenido de las hojas y se mezcló en cinco proporciones. Luego, se realizó el tamizaje fitoquímico a los extractos hidro-alchólicos obtenidos de las muestras. Resultados: el tamizaje fitoquímico de los polvos mixtos no detectó resinas, ni flavonoides, excepto en la muestra 4, que se observó este último metabolito. Las quinonas abundan en todas las muestras, excepto en el ensayo 1, que se detectó poca presencia. Además, las proporciones 1, 2 y 3 presentaron saponinas y los alcaloides se identificaron en las muestras 1, 4 y 5, así como, las coumarinas se observaron en las muestras con mayores proporciones de las plantas individuales. Los otros metabolitos secundarios se detectaron con baja presencia en todas las mezclas. Conclusiones: de acuerdo al análisis preliminar de los metabolitos secundarios de los polvos mixtos de hojas de P. guajava, A. occidentale, M. oleifera y M. citrifolia, se sugiere el estudio de estas mezclas medicinales como aditivo fitoquímico en las dietas de los animales, como primer estudio biológico(AU)


Introduction: Phytochemical screening of a plant provides insight into its potential medicinal uses. Phytochemical data about the leaves of Anacardium occidentale L., Psidium guajava L., Morinda citrifolia L. and Moringa oleifera Lam. may be found in the literature. However, no information was found about the mixed powder of these leaves. Objective: Perform phytochemical screening of mixed powders of leaves of A. occidentale, P. guajava, M. citrifolia and M. oleifera to determine their secondary metabolites. Methods: Leaves of the study plants were collected, dehydrated at ambient and room temperature and crushed to 1 mm particles. The study samples were formed by mixing the dry powder from the leaves at five proportions. Phytochemical screening was then conducted of the hydroalcoholic extracts obtained from the samples. Results: Phytochemical screening of the mixed powders did not detect any resins or flavonoids, except in sample 4, where the latter metabolite was found. Quinones are abundant in all samples, except for assay 1, where they were scant. Proportions 1, 2 and 3 contained saponins; alkaloids were identified in samples 1, 4 and 5; and coumarines were found in the samples with greater proportions of individual plants. The remaining secondary metabolites had a low presence in all the mixtures. Conclusions: Based on the preliminary analysis of secondary metabolites in mixed powders of leaves of P. guajava, A. occidentale, M. oleifera and M. citrifolia, it is suggested to study these medicinal mixtures as phytochemical additives in animal feed as a first biological study(AU)


Subject(s)
Plants, Medicinal , /therapeutic use , Moringa oleifera/chemistry , Psidium/chemistry , Morinda/chemistry , Plant Leaves
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