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Article in Chinese | WPRIM | ID: wpr-879874


OBJECTIVE@#To study the association of serum levels of trace elements with core symptoms in children with autism spectrum disorder (ASD).@*METHODS@#From September 2018 to September 2019, an investigation was performed for 1 020 children with ASD and 1 038 healthy children matched for age and sex in the outpatient service of grade A tertiary hospitals and special education institutions in 13 cities of China. Autism Behavior Checklist (ABC), Social Responsiveness Scale (SRS), and Childhood Autism Rating Scale (CARS) were used to assess the core symptoms of the children with ASD. The inductively coupled plasma mass spectrometry was used to measure serum levels of trace elements magnesium, iron, copper, and zinc.@*RESULTS@#The children with ASD had significantly lower serum levels of magnesium, copper, and zinc than the healthy children (@*CONCLUSIONS@#The serum levels of magnesium and zinc may be associated with core symptoms in children with ASD, which requires further studies. The nutritional status of trace elements should be monitored for children with ASD in clinical practice.

Autism Spectrum Disorder , Child , China , Copper/analysis , Humans , Trace Elements/analysis , Zinc
Article in Chinese | WPRIM | ID: wpr-888023


The effects of Chloriti Lapis on metal elements in plasma and lung tissue of acute exacerbation of chronic obstructive pulmonary disease( AECOPD) rats were studied. The rat AECOPD model with phlegm heat syndrome was established by smoking combined with Klebsiella pneumoniae infection. After the rats were treated by Chloriti Lapis,the contents of metal elements in plasma and lung tissue were determined by inductively coupled plasma-optical emission spectroscopy( ICP-OES) and inductively coupled plasma mass spectrometry( ICP-MS). The changes in the contents of metal elements were analyzed by SPSS 18. 0. Further,the correlations of differential metal elements( including Cu/Zn ratio) with differential metabolites in plasma,lung tissue and urine of AECOPD rats treated with Chloriti Lapis were analyzed. The results showed that Chloriti Lapis significantly up-regulated the contents of Fe,Al,Mn,Cu,Zn,Sn( P<0. 05),V,Co( P< 0. 01) and Cu/Zn ratio( P< 0. 05),and significantly down-regulated the contents of Ti( P< 0. 05)and Pb( P<0. 05) in the model rat plasma. It significantly increased the content of Be( P<0. 05) and decreased the contents of Mg,Ti and Al( P<0. 01) in model rat lung tissue. The element profiles of normal group,model group and Chloriti Lapis group can be well separated. Chloriti Lapis group and other groups were clustered into two categories. The taurine in plasma and phytosphingosine in lung tissue had the strongest correlations with differential metal elements. The Fe,Al,Mg,Be,Ti,V,Mn,Cu,Zn,Sn,and Co in Chloriti Lapis may directly or indirectly participate in the intervention of AECOPD rats. This group of metal elements may be the material basis of Chloriti Lapis acting on AECOPD rats,and reduce the Cu/Zn value in vivo. It was further confirmed that Chloriti Lapis could interfere with the metabolic pathways of taurine and hypotaurine in plasma and urine as well as the sphingolipid metabolism pathway in lung tissue of AECOPD rats. In addition,this study confirmed that long-term smoking can cause high-concentration Cd accumulation in the lung and damage the lung tissue.

Animals , Lung , Medicine, Chinese Traditional , Minerals , Pulmonary Disease, Chronic Obstructive , Rats , Spectrum Analysis , Trace Elements/analysis
Rev. cuba. pediatr ; 92(2): e649, abr.-jun. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1126742


Objetivo: Comparar las concentraciones de los oligoelementos cobre y zinc en la leche materna, de vaca y de cabra. Métodos: Estudio descriptivo de corte transversal. Se recogieron muestras de leche materna de madres lactantes sanas (91 mujeres) que asistieron a un centro materno infantil en la ciudad de Maracaibo-Venezuela. Previo consentimiento informado se seleccionaron madres que cumplieron con los criterios de inclusión. Las concentraciones de los oligoelementos en estudio, se determinaron mediante espectrofotometría de absorción atómica con llama. Resultados: En el análisis de la muestra de leche humana se hallaron concentraciones de cobre de 0,290 ± 0,04 mg/L y concentraciones de zinc de 1,580 ± 0,35 mg/L. Estos valores son considerados aceptables según la ingesta diaria de referencia para estos oligoelementos y fueron más elevada en muestras en leche de cabra según la literatura, mientras que las concentraciones de zinc en ambas leches (cabra y vaca), registradas por otros especialistas, fueron más elevadas que en la leche humana del presente estudio. Conclusiones: Las concentraciones de cobre y zinc halladas en la leche materna en este trabajo son las ingestas recomendadas para el desarrollo y madurez del sistema nervioso central del neonato(AU)

Objective: To compare the concentrations of trace elements like copper and zinc present in breast, cow's and goat's milk. Methods: Descriptive, cross sectional study. Samples of breast milk were collected from healthy lactating mothers (91 women) who attended a maternal and children health center in Maracaibo city, Venezuela. Mothers were selected prior informed consent who met the inclusion criteria. The concentrations of the studied trace elements were determined by atomic absorption spectrophotometry with flame. Results: In the analysis of the human milk´s sample, there were found concentrations of Cu (0.290 ± 0.04 mg/L) and concentrations of Zn (1.580 ± 0.35 mg/L). These levels are considered acceptable according to the permitted intake of trace elements and according to the literature, those are higher in goat milk´s samples, while concentrations of Zn in both milks (goat and cow), registered by other specialists were higher than in the human milk´s of the present study. Conclusions: The concentrations of copper and zinc found in breast milk are the recommended daily intake for the development and maturity of the central nervous system of the newborns(AU)

Humans , Female , Adult , Trace Elements/analysis , Dietary Minerals/administration & dosage , Milk, Human/chemistry , Goats , Epidemiology, Descriptive , Cross-Sectional Studies , Breast-Milk Substitutes
Cienc. tecnol. salud ; 6(2): 132-148, jul dic 2019. ^c27 cmilus
Article in Spanish | LILACS | ID: biblio-1095876


Los oligoelementos son importantes constituyentes nutricionales de las hierbas comestibles. Se colectaron 11 especies, nueve nativas (Amaranthus hybridus, Cnidoscolus aconitifolius, Crotalaria longirostrata, Dysphania ambrosioides, Lycianthes synanthera, Sechium edule, Solanum americanum, S. nigrescens, S. wendlandii) y dos introducidas (Moringa oleifera, Spinacea oleracea) en dos regiones de Guatemala. Se prepararon muestras de la hierba seca, cocida y de caldo de hierba fresca. Se cuantificaron por espectrofotometría de absorción atómica los macro (N, P, K) y oligoelementos (Ca, Mg, Na, Cu, Zn, Mn), taninos por espectrofotometría y oxalatos por permanganimetría. El contenido de oligoelementos es diverso, para Zn, la hierba control S. oleracea contienen buena cantidad (90-140 ppm); de las nativas D. ambrosioides (130-160 ppm) y A. hybridus (70-80 ppm) tienen la mayor cantidad. Respecto a Fe las hierbas control tienen buena composición (S. oleracea, 220-280 ppm y M. oleifera, 105-135 ppm); de las nativas A. hybridus (90-240 ppm), C. aconitifolius (75-185 ppm) y L. synanthera (75-140 ppm) tienen buenas concentraciones. Se encontraron niveles elevados de oxalatos en S. oleracea (67.30 (5.51) mg/g), L. synanthera (56.30 (9.67) mg/g) y S. nigrescens (33.6 (5.48) mg/g); en las demás hierbas se encontraron niveles menores. Los niveles de taninos fueron bajos (0.1-0.8 mg/g) para todas las especies. Se demuestra que cuatro especies nativas tienen un buen contenido de oligoelementos y presentan valores menores de antinutricionales que los controles.

Trace elements are important nutritional constituents from edible herbs. Eleven species were collected in two regions of Guatemala, nine native (Amaranthus hybridus, Cnidoscolus aconitifolius, Crotalaria longirostrata, Dysphania ambrosioides, Lycianthes synanthera, Sechium edule, Solanum americanum, S. nigrescens, S. wendlandii) and two introduced (Moringa oleifera, Spinacea oleracea). Dry, cooked and broth samples were prepared. By atomic absorption spectrometry, macro (N, P, K) and trace elements (Ca, Mg, Na, Cu, Zn, Mn) were quantified, tannins by spectrophotometry, and oxalates by permanganometry. Trace elements content is diverse, for Zn, control herb S. oleracea contained high quantity (90-140 ppm); from the natives D. ambrosioides (130-160 ppm) and A. hybridus (70-80 ppm) contained high amounts. For Fe, control herbs had high composition (S. oleracea, 220-280 ppm, M. oleifera, 105-135 ppm); from the natives A. hybridus (90-240 ppm), C. aconitifolius (75-185 ppm) and L. synanthera (75-140 ppm) had the highest amounts. High levels of oxalates were demonstrated in S. oleracea (67.30 (5.48) mg/g), L. synanthera (56.30 (9.67) mg/g), and S. nigrescens (33.6 (5.48) mg/g); from the others levels.

Trace Elements/analysis , Vegetables/classification , Tannins/analysis , Trace Elements/administration & dosage , Amaranthus/chemistry , Jatropha/chemistry , Malnutrition/prevention & control
Arq. bras. med. vet. zootec. (Online) ; 71(4): 1227-1235, jul.-ago. 2019. tab, graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1038598


O objetivo deste estudo foi avaliar o efeito metafilático de minerais traços e vitaminas A e E injetáveis, em parâmetros do estresse oxidativo e na função de neutrófilos em fêmeas da raça Holandesa no período de transição. Sessenta animais foram divididos em dois grupos: animais que receberam minerais traços e vitaminas A e E injetáveis (GMTV) (n= 30) e grupo controle (GC) (n= 30). Não houve diferença significativa entre os grupos nos parâmetros avaliados, porém observou-se diferença significativa entre tratamento e dia para os valores da SOD, com maior atividade dessa enzima em fêmeas GMTV, nas semanas próximas ao parto. Observou-se diferença de dia e para interação tratamento e dia para o TBARS, em que fêmeas GMTV mostraram menores valores de TBARS em todos os momentos, exceto sete dias após o parto. Houve efeito significativo de dia para leucócitos, interação tratamento e dia para neutrófilos e interação tratamento e dia para fagocitose de neutrófilos, em que as fêmeas GMTV apresentaram valores menores de leucócitos e neutrófilos próximo ao parto, além de maior fagocitose de neutrófilos. Pode-se observar que houve melhora no sistema oxidativo e imune de fêmeas GMTV, resposta que provavelmente está relacionada com a administração dos minerais traços e vitaminas A e E.(AU)

This study evaluates the metaphylactic of the subcutaneous administration of a trace minerals and vitamins A and E, on the oxidative stress and neutrophil function in Holsteins cows under the transition period. Sixty females were divided in two groups: group with trace minerals and vitamins (GMTV) (n= 30) and group control (GC) (n= 30). There was no significant difference between those groups; however, we find significant difference between treatment and day for Superoxide dismutase (SOD) values with higher activity of this enzyme in females GMTV on the weeks next to the parturition. Still, there was difference on day and, for interactions between treatment and day for TBARS, were females GMTV showed lower values of TBARS in all moments, except on day seven after the parturition. For leucocytes, there is a significant effect by day, interaction on treatment and day on neutrophils, and interaction treatment and day for neutrophil phagocytosis, were females GMTV showed lower values of leucocytes and neutrophils next to the parturition, and an increase of neutrophils phagocytosis. In summary, cows from the GMTV group had an improvement on the immune and oxidative systems, probably correlated with the administration of this supplement.(AU)

Animals , Female , Cattle , Trace Elements/analysis , Vitamin A/administration & dosage , Vitamin E/administration & dosage , Oxidative Stress , Neutrophils , Injections, Subcutaneous/veterinary
Arq. bras. oftalmol ; 82(2): 141-148, Mar.-Apr. 2019. tab
Article in English | LILACS | ID: biblio-989402


ABSTRACT Purpose: We aimed to compare the body mass index and vitamin and mineral status of children with and without amblyopia. Methods: Amblyopic children aged between 5 and 18 years (n=46) and age-matched control children (n=32) were evaluated in terms of anthropometric parameters, including height, weight, body mass index and demographic features. Serum vitamin B12 and folate were measured using an Advia Centaur XP (Siemens, Ireland) biochemistry analyzer. We evaluated the inorganic mineral elements from hair samples with inductively coupled plasma-mass spectrometry using a Thermo XSeries 2 analyzer (Thermo Fisher Scientific, Bremen, Germany). Results: No significant difference was found between the two groups in terms of height, weight, and body mass index or serum B12 and folate concentrations (p>0.05). Children with severe amblyopia had lower vitamin B12 and folate and higher body mass index. The levels of phosphorus (p=0.012), selenium (p=0.002), molybdenum (p<0.001), iodine (p=0.002), chromium (p=0.022), boron (p<0.001), and beryllium (p=0.005) were all significantly lower in the amblyopia group compared to the control group. All of these minerals, except phosphorus, were also significantly lower in those with severe amblyopia compared to those with milder amblyopia and controls (p<0.05). Conclusion: Amblyopic children are significantly deficient in some inorganic elements. Inorganic elements, vitamin B12, and folate may play an important role in the visual development of amblyopic children.

RESUMO Objetivo: Nosso objetivo foi comparar o índice de massa corporal e o nível de vitaminas e minerais de crianças com e sem ambliopia. Métodos: Crianças amblióticas com idades entre 5 e 18 anos (n=46) e crianças controle pareadas por idade (n=32) foram avaliadas quanto a parâmetros antropométricos, incluindo altura, peso, índice de massa corporal e características demográficas. A vitamina B12 e o folato séricos foram medidos utilizando um analisador bioquímico Advia Centaur XP (Siemens, Irlanda). Avaliamos os elementos minerais inorgânicos de amostras de cabelo com espectrometria de massa de plasma indutivamente acoplado usando um analisador Thermo XSeries 2 (Thermo Fisher Scientific, Bremen, Alemanha). Resultados: Não houve diferença significativa entre os dois grupos em relação à altura, peso e índice de massa corporal ou concentrações séricas de B12 e folato (p>0,05). Crianças com ambliopia severa tinham menor vitamina B12 e folato e maior índice de massa corporal. Os níveis de fósforo (p=0,012), selênio (p=0,002), molibdênio (p<0,001), iodo (p=0,002), cromo (p=0,022), boro (p<0,001) e berílio (p=0,005) foram todos significativamente menores no grupo com ambliopia em comparação com o grupo controle. Todos esses minerais, exceto o fósforo, também foram significativamente menores naqueles com ambliopia em comparação com aqueles com ambliopia leve e grupo controle (p<0,05). Conclusão: As crianças amblíopes são significativamente deficientes em alguns elementos inorgânicos. Elementos inorgânicos, vitamina B12 e folato podem desempenhar um papel importante no desenvolvimento visual de crianças com ambliopia.

Humans , Male , Female , Child , Vitamin B 12/blood , Amblyopia/physiopathology , Amblyopia/blood , Nutritional Status/physiology , Folic Acid/blood , Hair/chemistry , Reference Values , Trace Elements/analysis , Body Mass Index , Case-Control Studies , Anthropometry , Prospective Studies , Analysis of Variance , Statistics, Nonparametric
An. acad. bras. ciênc ; 90(1): 485-494, Mar. 2018. tab, graf
Article in English | LILACS | ID: biblio-886916


ABSTRACT Soybean (Glycine max L.) seed contains amounts of protein, lipid, carbohydrate and mineral elements, which protein and lipid have been known as a main part for soybean's trade value. In this study, in order to investigate the effect of ferrous nano-oxide particles on nutritional compounds of soybean seed, an experiment with 5 treatments and 3 replications was conducted as a randomized complete block design. Treatments were 5 concentrations of ferrous nano-oxide particles including 0, 0.25, 0.5, 0.75 and 1 g L-1 which were sprayed 3 times at 4 and 8 leaves stage and pod initiation. Lipid and protein contents, fatty acids profile, some of mineral elements such as Fe, Mg, Ca and P, chlorophyll a, b and total chlorophyll content were determined. Results showed that solution containing ferrous nano-oxide particles had significant effect on nutritional compounds of soybean seed (P<0.01) compared to control. The highest content of lipid and protein (25.4 and %33.8, respectively) observed by applying 0.75 g L-1 of ferrous nano-oxide and the lowest content was also in control. Changes in the trends of fatty acids profile (palmitic, oleic, linoleic and linolenic acids), some of mineral elements (Fe, Mg, Ca and P) and chlorophyll contents were similar to lipid and protein levels which by increasing in concentration of ferrous nano-oxide from 0 to 0.75 g L-1 all measured parameters also increased, but reduction in all parameters was observed in concentration from 0.75 to 1 g L-1. In conclusion, application of 0.75 to 1 g L-1 ferrous nano-oxide had the best effect on the nutrient composition of soybean seed.

Seeds/drug effects , Seeds/chemistry , Soybeans/drug effects , Soybeans/chemistry , Ferrous Compounds/pharmacology , Metal Nanoparticles/chemistry , Reference Values , Seeds/physiology , Soybeans/physiology , Trace Elements/analysis , Proteins/analysis , Random Allocation , Chlorophyll/analysis , Regression Analysis , Reproducibility of Results , Fatty Acids/analysis , Fertilizers , Lipids/analysis
An. acad. bras. ciênc ; 90(1): 557-571, Mar. 2018. tab, graf
Article in English | LILACS | ID: biblio-886913


ABSTRACT Native to subtropical region of South America, yerba mate is responsive to P under some conditions, but the degree of influence of genetic and soil on the growth and composition of the leaf is unknown. The aim of study was to evaluate plant growth, nutrients and potentially toxic elements in leaves of yerba mate clones in response to P application in acid soils. In greenhouse condition, two yerba mate clone seedlings were grown (210 days) in pots, each clone in a completely randomized design in factorial scheme (with and without P; four acid soils). The elemental composition of leaves and the growth of plants were determined. Phosphorus promoted plant growth, but this was not accompanied by increased P in leaf tissue in all conditions tested. The P effect on the elemental composition varied: decrease/null (N, K, Mg, Mn, Cu, Ni, B, Mo, Al, Cd); increase/null (C/N, C, Ca, Fe, V); increase/decrease/null (Zn, Ba, Pb) and; null (Cr). The soils affect the elemental composition of the leaves, especially Mn, with accumulation greater than 1000 mg kg-1. The Ba, Pb, Al and Zn in the leaves varied among clones. Yerba mate response to P was affected by edaphic and plant factors.

Phosphorus/chemistry , Soil/chemistry , Clone Cells/chemistry , Plant Leaves/chemistry , Ilex paraguariensis/growth & development , Ilex paraguariensis/chemistry , Reference Values , Seeds/growth & development , Seeds/drug effects , Time Factors , Trace Elements/analysis , Random Allocation , Plant Leaves/drug effects , Ilex paraguariensis/drug effects , Fertilizers , Plant Development/drug effects
Braz. j. biol ; 76(4): 967-974, Oct.-Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-828099


Abstract In this study we determined the concentration of metals (Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) in the water lower São Francisco River basin, to evaluate the influence of urbanization and industrialization on environmental changes in the water resource. All samples were analyzed using the IUPAC adapted method and processed in an atomic absorption spectrophotometer. The sampling stations located near the industrial areas were influenced by industrialization because they presented higher concentrations of Cd, Cr, Ni and Cu. The other sampled locations showed changes with regard the trace elements probably originating in the soil, like Fe, Zn and Pb. There was a gradual increase in the concentrations of metals, in general, in the period of highest rainfall of the hydrographic network. Overall, except for Zn and Mn, the trace elements exceeded the maximum allowed value established by national legislation (CONAMA). Lower São Francisco River basin has suffered interference from urbanization and industrialization, so awareness programs should be developed so as to control and lessen future problems.

Resumo Neste trabalho foi determinada a concentração dos metais (Cd, Cr, Cu, Fe, Mn, Ni, Pb e Zn) na água do submédio Rio São Francisco para avaliar a influência da urbanização e da industrialização nas modificações ambientais do recurso hídrico. Todas as amostras foram analisadas usando o método IUPAC adaptado e processados em um espectrofotômetro de absorção atômica. As estações de amostragem localizadas próximas às áreas industriais sofreram influência da industrialização por apresentarem concentrações mais elevadas de Cd, Cr, Ni e Cu. Os demais locais amostrados apresentaram modificações com relação aos elementos traços de provável origem sedimentar como Fe, Zn e Pb. Houve aumento gradativo nas concentrações dos metais, de forma geral, no período de maior pluviosidade da rede hidrográfica. Em geral, com exceção do Zn e Mn, os elementos traços ultrapassaram o valor máximo permitido, estabelecido pela legislação nacional (CONAMA). O submédio Rio São Francisco tem sofrido interferência da urbanização e industrialização, por isso, é preciso que, programas de conscientização sejam estabelecidos, para controlar e amenizar problemas futuros.

Seasons , Metals, Heavy/analysis , Rivers/chemistry , Spectrophotometry, Atomic , Trace Elements/analysis , Urbanization , Brazil , Environmental Monitoring , Industrial Development
Braz. j. biol ; 76(4): 871-877, Oct.-Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-828081


Abstract Concentrations of ten elements (Cd, Cr, Cu, Fe, Ni, Pb, Se, Sr, V and Zn) were determinate in muscle tissues of 13 fish species from Aratu Bay, Bahia, Brazil by inductively coupled plasma optical emission spectrometry. The accuracy and precision of our results were checked by using two certified reference materials: BCR-422 cod muscle and SRM 1566b oyster tissue. The average trace element concentrations in the fish species varied in the following ranges, in μg g–1: 0.03-0.8 for Cr; 2.0-33.7 for Cu, 2.4-135.1 for Fe, 1.6-25.6 for Se; 1.6-35.1 for Sr; and 2.8-40.5 for Zn. The Diaptereus rhombeus (carapeba) specie presented the highest concentrations of Se, Cu and Fe. Chromium and Se were present at levels above the limit of tolerance allowed by the National Agency of Sanitary Vigilance (ANVISA). The results were also evaluated using the multivariate analysis techniques: principal component analysis (PCA) and hierarchical cluster analysis (HCA).

Resumo Foram determinadas as concentrações de dez elementos (Cd, Cr, Cu, Fe, Ni, Pb, Se, Sr, V and Zn) em tecidos de músculos de treze espécies de peixes da Baía de Aratu, Bahia, Brasil, utilizando a espectrometria de emissão ótica com plasma indutivamente acoplado. A precisão e exatidão dos resultados foram analisadas utilizando dois materiais de referência certificada: BCR-422 cod muscle e o SRM 1566b oyster tissue. A concentração média dos elementos traço nas espécies de peixes variaram nas seguintes faixas, em μg g−1: Cr = 0,03-0,8; Cu = 2,0-33,7, Fe = 2,4-135,1, Se = 1,6-25,6; Sr = 1,6-35,1; Zn = 2.8-40.5. A espécie Diaptereus rhombeus (carapeba) apresentou as maiores concentrações de Se, Cu e Fe. Cromo e Se apresentaram níveis acima do limite de tolerância estabelecido pela Agência Nacional de Vigilância Sanitária (ANVISA). Os resultados ainda foram avaliados usando técnicas de análises multivariadas: análise de componente principal (PCA) e análise de agrupamento hierárquico (HCA).

Animals , Trace Elements/analysis , Seafood/analysis , Fishes , Brazil , Food Contamination , Cluster Analysis , Bays , Principal Component Analysis , Metals/analysis , Muscles/chemistry
Pesqui. vet. bras ; 34(11): 1141-1145, nov. 2014. ilus, tab
Article in English | LILACS, VETINDEX | ID: lil-736042


A number of studies has shown that antioxidants, fatty acids and trace minerals may modulate different immune cell activities, and that their deficiency may be associated with diseases and impaired immune responses. In innate immunity, natural killer (NK) cells have a central role, killing virally infected and cancerous cells, and also secreting cytokines that shape adaptive immune responses. Thus, the aim of this study was to evaluate the effect of enriched diets in selenium plus vitamin E and/or canola oil on complete blood count and on NK cell cytotoxicity from blood lymphocytes of Nellore bulls. Bulls that received selenium plus vitamin E had (P=0.0091) higher NK cell cytotoxicity than control bulls. This result positively correlated with serum selenium levels. To the best of our knowledge, this is the first study that showed immunostimulatory effects of selenium plus vitamin E on NK cell cytotoxicity of Nellore bulls.(AU)

Vários estudos demonstraram que antioxidantes, ácidos graxos e minerais podem modular a atividade de diferentes células do sistema imunológico e que as suas carências podem estar associadas a doenças e a respostas imunes comprometidas. Na imunidade inata, os linfócitos natural killer (NK) têm um papel central matando células infectadas por vírus e células cancerígenas, ao mesmo tempo em que também secretam citocinas que modulam as respostas imunes adaptativas. Assim, o objetivo deste estudo foi avaliar o efeito de dietas enriquecidas em selênio e vitamina E e/ou óleo de canola no hemograma e na citotoxicidade das células NK do sangue de bovinos da raça Nelore. Os animais que receberam selênio e vitamina E tiveram (P = 0,0091) maior citotoxicidade das células NK do que os animais do grupo controle. Este resultado foi positivamente correlacionado com os níveis de selênio no sangue. Para o melhor do nosso conhecimento, este é o primeiro estudo que mostrou efeitos imunoestimulatórios do selênio e vitamina E sobre a citotoxicidade das células NK de bovinos Nelore.(AU)

Animals , Cattle , Selenium/administration & dosage , Vitamin E/administration & dosage , Killer Cells, Lymphokine-Activated/drug effects , Cytotoxins/analysis , Trace Elements/analysis , Immunization/veterinary , Dietary Supplements/analysis , Diet/veterinary
Biol. Res ; 47: 1-14, 2014. graf, tab
Article in English | LILACS | ID: biblio-950719


BACKGROUND: In recent years, the desire to adopt a healthy diet has drawn attention to legume seeds and food products derived from them. Mash bean is an important legume crop used in Pakistan however a systematic mapping of the chemical composition of mash bean seeds is lacking. Therefore seeds of four mash bean (Vigna mungo (L.) Hepper, family Leguminoseae) cultivars (NARC-Mash-1, NARC-Mash-2, NARC-Mash-3, NARC-Mash-97) commonly consumed in Pakistan have been analyzed for their chemical composition, antioxidant potential and biological activities like inhibition of formation of advanced glycation end products (AGE) activity and tyrosinase inhibition activity. RESULTS: The investigated cultivars varied in terms of biochemical composition to various extents. Mineral composition indicated potassium and zinc in highest and lowest amounts respectively, in all cultivars. The amino acid profile in protein of these cultivars suggested cysteine is present in lowest quantity in all cultivars while fatty acid distribution pattern indicated unsaturated fatty acids as major fatty acids in all cultivars. All cultivars were found to be rich source of tocopherols and sterols. Fourier transform infrared spectroscopy (FTIR) fingerprints of seed flour and extracts indicated major functional groups such as polysaccharides, lipids, amides, amines and amino acids. Results indicated that all investigated cultivars possessed appreciable antioxidant potential. CONCLUSIONS: All cultivars are rich source of protein and possess sufficient content of dietary fiber, a balanced amino acid profile, low saturated fatty acids and antioxidant capacity that rationalizes many traditional uses of seeds of this crop besides its nutritional importance. The collected data will be useful for academic and corporate researchers, nutritionists and clinical dieticians as well as consumers. If proper attention is paid, it may become an important export commodity and may fetch considerable foreign exchange for Pakistan.

Seeds/chemistry , Crops, Agricultural/chemistry , Fabaceae/chemistry , Pakistan , Phenols/analysis , Riboflavin/analysis , Seeds/metabolism , Sterols/analysis , Tannins/analysis , Thiamine/analysis , Trace Elements/analysis , Flavonoids/analysis , Plant Extracts/chemistry , Free Radical Scavengers , Monophenol Monooxygenase/antagonists & inhibitors , Glycation End Products, Advanced/metabolism , Crops, Agricultural/metabolism , Reducing Agents/metabolism , Cysteine/analysis , Tocopherols/analysis , Fabaceae/metabolism , Niacin/analysis , Antioxidants/metabolism , Nutritive Value
Egyptian Journal of Hospital Medicine [The]. 2013; 52: 599-607
in English | IMEMR | ID: emr-170289


Acrylonitrile [a chemical pollutant] has been reported to induce harmful effects in humans. Therefore, this study was designed to evaluate the protective effects of hesperidin, a natural bioflavonoid, against the toxicity induced by acrylonitrile [AN] in rats. This study includes determination of serum total scavenger capacity "TSC", liver enzymes [aspartate transaminase "ASAT", alanine transaminase "ALAT" and alkaline phosphatase "ALP"], total proteins, albumin, glucose, creatinine, urea and lipid profile. Moreover, liver and kidney homogenate glutathione content "GSH", catalase, superoxide dismutase "SOD", glutathione peroxidase "GPx", malondialdehyde "MDA" and some minerals were estimated. Revealed that administration of AN [orally 50mg/ kg b.wt.] induced alterations in TSC level as well as liver, kidney and lipid profiles. In addition, a decrease in GSH-content and catalase, SOD and GPx activities was observed with an increase in MDA levels in both liver and kidney. There was disturbance in certain minerals such as Cu, Zn, Fe, Se, Ca, Mg and Mn. Particularly, Hesperidin administration [orally 200 mg/kg b.wt.] ameliorates the oxidative stress induced by AN, consistent with the reported antioxidant activity of hesperidin

Animals, Laboratory , Trace Elements/analysis , Liver Function Tests , Kidney Function Tests , Oxidative Stress , Hesperidin , Protective Agents , Treatment Outcome , Rats
Rev. chil. cardiol ; 32(3): 214-220, 2013. ilus, tab
Article in Spanish | LILACS | ID: lil-705224


Introducción: La población de la Región de Antofagasta se encuentra expuesta a contaminación ambiental por metales pesados (MP). Los elementos traza (ET) podrían actuar con As como agonistas o antagonistas. El presente estudio detalla la determinación de las concentraciones totales (CT) de V, Cr, Mn, Co, Ni, Cu, As, Se y Boro en aurícula derecha (AD), arteria mamaria izquierda (AMI), vena safena (VS) y tejido adiposo (TA) de personas que padecen patologías cardiovasculares (CV) y han requerido cirugía cardíaca. Métodos: Se analizaron AMI, VS, AUD, y TA en un grupo de 50 pacientes con cardiopatía coronaria de la Región de Antofagasta que han sido sometidos a cirugía cardíaca (grupo expuesto, E), y en un grupo de 20 pacientes control sometidos a cirugía cardíaca en el mismo período en Santiago (grupo no expuesto, NE). Las determinaciones de ET y MP se realizaron mediante Técnicas Ópticas y Atómicas. Resultados: En el grupo de pacientes E residentes en la Región de Antofagasta Boro presenta correlaciones interelementales significativas (p=0,05), que no se presentan o son escasas en el grupo NE. El rango de CT de B en AD fue de 24,721 - 932,032 μg/g, niveles muy superiores a los encontrados en el resto de los elementos en estudio. El rango de CT de B encontrada para AD fue de 25,205 - 56,873 μg/g. Conclusión: Individualmente, el elemento más enriquecido en todos los tipos de tejidos del grupo E de la Región de Antofagasta es B, le siguen As y Mn. Cu está empobrecido en el grupo NE, en particular en AD y AMI.

The Antofagasta population is exposed to environmental contamination with heavy metals (HM). Trace elements (TE) might interact with As either as agonists or antagonists. The total concentracions of V, Cr, Mn, Co, Ni, Cu, Se and B in the right atrium (RA), left internal mammary artery (LIMA), saphenous vein (SV) and adipose tissue (AT) from patients undergoing cardiac surgery are reported. Methods: LIMA, SV, RA and AT from 50 patients with myocardial infarction submitted to revascularization surgery in Antofagasta (exposed group, E) or Santiago (non exposed group NE, 20 patients) were analyzed. Optical and atomic techniques were used for analysis of HM and TE. Results: Significant inter elemental correlations were observed for B in group E, (p<0.05), which are not present in group NE. The RA concentrarion of B in group A ranged from 24.72 to 932.03 ug/g, substancially higher than concentracion of the other elements under study. Corresponding concentra-rions of B in the NE group were 25.21 to 56. 87 ug/g. Conclusion: B was the most enriched element in the exposed group, followed by As and Mn. Cu content was significantly lower in RA and LIMA from patients in the non exposed group.

Humans , Male , Female , Middle Aged , Environmental Pollution/adverse effects , Metals, Heavy/adverse effects , Trace Elements/analysis , Chile
Braz. j. med. biol. res ; 45(8): 716-720, Aug. 2012. tab
Article in English | LILACS | ID: lil-643659


The objective of this study was to evaluate the effect of short-term levosimendan exposure on oxidant/antioxidant status and trace element levels in the testes of rats under physiological conditions. Twenty male Wistar albino rats were randomly divided into two groups of 10 animals each. Group 1 was not exposed to levosimendan and served as control. Levosimendan (12 µg/kg) diluted in 10 mL 0.9% NaCl was administered intraperitoneally to group 2. Animals of both groups were sacrificed after 3 days and their testes were harvested for the determination of changes in tissue oxidant/antioxidant status and trace element levels. Tissue malondialdehyde (MDA) was significantly lower in the levosimendan group (P < 0.001) than in the untreated control group and superoxide dismutase and glutathione peroxidase (GSH-Px) levels were significantly higher in the levosimendan group (P < 0.001). Carbonic anhydrase, catalase and GSH levels were not significantly different from controls. Mg and Zn levels of testes were significantly higher (P < 0.001) and Co, Pb, Cd, Mn, and Cu were significantly lower (P < 0.001) in group 2 compared to group 1. Fe levels were similar for the two groups (P = 0.94). These results suggest that 3-day exposure to levosimendan induced a significant decrease in tissue MDA level, which is a lipid peroxidation product and an indicator of oxidative stress, and a significant increase in the activity of an important number of the enzymes that protect against oxidative stress in rat testes.

Animals , Male , Rats , Antioxidants/pharmacology , Hydrazones/pharmacology , Malondialdehyde/metabolism , Oxidative Stress/drug effects , Pyridazines/pharmacology , Reactive Oxygen Species/metabolism , Trace Elements/analysis , Glutathione Peroxidase/metabolism , Random Allocation , Rats, Wistar , Superoxide Dismutase/metabolism
Article in English | WPRIM | ID: wpr-119899


We measured selenium, zinc, copper and manganese concentrations in the human milk of Korean mothers who gave birth to preterm infants, and compared these measurements with the recommended daily intakes. The samples of human milk were collected postpartum at week-1, -2, -4, -6, -8, and -12, from 67 mothers who gave birth to preterm infants (< 34 weeks, or birth weight < 1.8 kg). All samples were analyzed using atomic absorption spectrophotometry. The concentrations of selenium were 11.8 +/- 0.5, 11.4 +/- 0.8, 12.7 +/- 0.9, 11.4 +/- 0.8, 10.8 +/- 0.9, and 10.5 +/- 1.3 microg/L, zinc were 7.8 +/- 0.5, 9.1 +/- 0.8, 7.2 +/- 0.9, 8.0 +/- 0.8, 7.4 +/- 0.9, and 6.6 +/- 1.2 mg/L, copper were 506 +/- 23.6, 489 +/- 29.4, 384 +/- 33.6, 356 +/- 32.9, 303 +/- 35.0, and 301 +/- 48.0 microg/L and manganese were 133 +/- 4.0, 127 +/- 6.0, 125 +/- 6.0, 123 +/- 6.0, 127 +/- 6.0, and 108 +/- 9.0 microg/L at week-1, -2, -4, -6, -8, and -12, respectively. The concentrations of selenium and zinc meet the daily requirements but that of copper is low and of manganese exceeds daily requirements recommended by the American Academy of Pediatrics, Committee on Nutrition.

Adult , Copper/analysis , Female , Humans , Infant, Newborn , Infant, Premature , Longitudinal Studies , Manganese/analysis , Milk, Human/chemistry , Postpartum Period , Republic of Korea , Selenium/analysis , Spectrophotometry, Atomic , Trace Elements/analysis , Zinc/analysis
International Journal of Occupational and Environmental Medicine. 2011; 2 (3): 143-156
in English | IMEMR | ID: emr-129900


Lead toxicity has been reported to affect hematopoietic, nervous, reproductive, cardiovascular and urinary tract systems. Many investigators have so far studied the effects of high blood lead levels on pregnancy outcomes. To investigate the effects of elevated maternal blood lead during pregnancy on some trace elements and pregnancy outcomes. Blood lead and plasma copper, iron and zinc were measured in 349 pregnant women with a mean_SD age of 27.0 +/- 4.8 years, and gestational age of 21.8 +/- 3.1 weeks, at recruitment using atomic absorption spectrophotometer. Maternal and fetal outcomes were recorded during follow-up and at delivery, respectively. A blood lead level of >10 pg/dL was considered high. Women with elevated blood lead had significantly higher plasma copper and iron and lower plasma zinc than women with low blood lead level [<10 microg/dL]. Blood lead level correlated with maternal hemoglobin concentration [r=-0.1054, p=0.051] and total white blood cell count [r=0.1045, p=0.053]. Hypertension, malaria and low birth weight were significantly higher [p<0.05] in women with elevated blood lead than in those with low blood lead level. Complications of pregnancy may be induced by a high blood lead level possibly through the alterations in trace element metabolism

Humans , Female , Adolescent , Adult , Lead/blood , Lead Poisoning , Pregnancy Outcome , Trace Elements/analysis , Nutritional Status , Trace Elements/blood
Behbood Journal. 2011; 14 (4): 316-327
in Persian | IMEMR | ID: emr-122339


Reactive oxygen species [ROS]-induced lipidperoxidation can lead to dysfunction of sperm and thereby, infertility may be occurred. So, always there is a balance between amount of ROS and anti-oxidant molecules in semen. Anti-oxidant enzymes of sperm; superoxide dismutase [SOD], glutathione peroxidase [GPX], catalse and zinc and selenium can protect it from destructive effects of ROS. Hence, the present study was designed to compare the activities of these enzymes and trace elements between fertile and idiopathic infertile men. Semen specimens were collected from 30 infertile men with proven infertility by an urologist, and 30 fertile men as control donors, with age range between 20-40 years old. Semen analysis was conducted by CASA method. Atomic absorption method was used for measuring of zinc and selenium concentration. Activity assays of SOD and GPX were performed by Randox Kits. Aebi method also was applied for evaluation of catalase activity. There was no difference between the activities of enzymes in fertile men and infertile ones. Also, it wasn't seen any difference in the selenium and zinc levels of seminal plasma. There was no relationship between evaluated items with sperm parameters. Only, in asthenoteratospermic individuals negative correlations were found between GPX and sperm motility, selenium and sperm morphology. Also, in these individuals, there was a positive correlation between SOD and catalse activity. Measuring activities of SOD, GPx, and catalase and the contents of zinc and selenium of seminal plasma do not appear to be suitable tools for determining the fertility potential of sperm

Humans , Male , Reactive Oxygen Species/metabolism , Spermatozoa/enzymology , Testis/enzymology , Glutathione Peroxidase/metabolism , Selenium/blood , Zinc/blood , Infertility, Male/etiology , Trace Elements/analysis , Antioxidants/metabolism
International Journal of Environmental Research. 2011; 5 (4): 847-854
in English | IMEMR | ID: emr-122637


A comprehensive study on the chemical composition of rainwater was carried out in Obuasi, a gold mining town in Ghana. Rainwater samples were analyzed for major ions [Ca[2+],Mg[2+], K[+], Na[+], NH[4+], Cr, NO[3-] and SO[4][2-] and trace metals [Fe, Al, Zn, Pb, Cr, and Cd]. The rainwater was typically acidic with a mean pH of 4.76 +/- 0.47, which ranged from 4.0 to 5.6. The low pH found in the water suggests that the concentration of the alkaline particles in the rainwater were not high enough to neutralize acidic species present. SO[4][2"] and CH were the main anions, while Ca[2+] and K[+] were the main cations. Concentrations of trace metals in this study were compared with those of other studies conducted at different sites in the world. The result of this study generally suggests that rainwater chemistry in the area is strongly influenced by local anthropogenic sources [mining]. The pollutants in rainwater samples were mainly derived from mining activities. Concentrations of Pb in all the samples were above the WHO permissible limit of 0.01 mg/L for drinking water

Mining , Trace Elements/analysis , Hydrogen-Ion Concentration
São Paulo; s.n; 2011. 159 p. ilus, tab, graf.
Thesis in Portuguese | LILACS | ID: lil-594177


A interação entre fragmentos de bicamada (BF) de brometo de dioctadecildimetilamônio (DODAB) e um mononucleotídeo-modelo (deoxiadenosina monofosfato, dAMP) ou um oligodeoxinucleotídeo-modelo (5'- AAAAAAAAAA-3', poli(dA)) ou um oligodeoxinucleotídeo terapêutico (5'- TTGACGTTCG -3', CpG) foi investigada por turbidimetria, espalhamento de luz dinâmico, espectroscopia de dicroísmo circular e de fluorescência e calorimetria diferencial de varredura (DSC). Respostas imunológicas foram caracterizadas com ensaio de hipersensibilidade tardia por inchamento de coxim patelar de camundongo, dosagem de anticorpos IgG1 e IgG2a e de citocinas secretadas por células de linfonodo em cultura. Poli(dA), em contraste com dAMP, induziu fusão máxima de DODAB BF a partir da neutralização de cargas, quando houve obtenção de um tamanho máximo e um potencial-zeta igual a zero para os arranjos. Para [poli(dA)] maiores do que aquela correspondente à neutralização de cargas, houve recuperação da estabilidade coloidal com reversão do potencial-zeta e com obtenção de tamanhos que foram aproximadamente o dobro daqueles determinados inicialmente para DODAB BF. A proporção molar de neutralização poli(dA): DODAB foi 1:10 para DODAB BF e 1:20 para vesículas grandes (LV) de DODAB, de acordo com as estruturas de bicamada aberta e fechada dessas duas dispersões de bicamada de DODAB. A fusão de DODAB BF induzida por poli(dA) foi extensiva aumentando o grau de empacotamento das bicamadas formadas conforme inferido a partir dos termogramas de DSC. Em condições de equivalencia de cargas, nucleotídeo não causou fusão de DODAB BF, mostrando a importância do caráter de polieletrólito do poli(dA) para induzir fusão. O sal divalente Na2HPO4 causou fusão e aumentou o empacotamento da bicamada graças à blindagem eficiente de cargas. Reestabilização coloidal como aquela induzida por poli(dA) não ocorreu em presença de Na2HPO4, NaCl ou nucleotídeo. Para complexos DODAB BF/CpG em presença de ovalbumina (OVA)...

The interaction between bilayer fragments (BF) of dioctadecyldimethylammonium bromide (DODAB) and a model nucleotide (deoxyadenosine monophosphate, dAMP) or a model oligodeoxynucleotide (5'- AAAAAAAAAA-3', poly(dA)) or a therapeutic oligodeoxynucleotide (5'- TTGACGTTCG -3', CpG) was investigated by means of turbidimetry, dynamic light scattering, circular dichroism and fluorescence spectroscopies and differential scanning calorimetry. Immune responses were characterized using footpad swelling delayed type hipersensitivity assay and antibody and cytokine measurements. In contrast to dAMP, poly(dA) induced maximal DODAB BF fusion from charge neutralization, where assemblies presented maximal size and zero zeta-potential. Above charge neutralization colloid stability was recovered with negative zeta-potentials and sizes that were about the double of those initially determined for DODAB BF. The poly(dA):DODAB molar ratio for neutralization was 1:10 for DODAB BF and 1:20 for DODAB LV, in agreement with the open and closed bilayer structures of these two DODAB bilayer dispersions. The poly(dA)-induced DODAB BF fusion was extensive and increased the packing of the formed bilayers, as inferred from DSC thermograms. In conditions of charge equivalence, nucleotide did not cause DODAB BF fusion, highlighting the importance of poly(dA)'s polyelectrolyte character to induce fusion. Divalent Na2HPO4 salt caused fusion and increased bilayer packing due to efficient BF charge shielding. Colloid restabilization as induced by poly(dA) was not observed in presence of Na2HPO4, NaCl and nucleotide. For DODAB BF/CpG complexes in presence of the ovalbumin (OVA) model antigen, the charge neutralization of DODAB BF/OVA by CpG reduced colloid stability, while charge overcompensation led to restabilization due to electrostatic repulsion, as observed for DODAB BF/poly(dA) interaction. Differences in size and neutralization proportions by CpG indicate that BF are able to load more OVA…

Animals , Mice , Adjuvants, Immunologic , Molecular Structure , Nucleotides/analysis , Trace Elements/analysis , Organic Cation Transport Proteins/immunology , Analysis of Variance , Bromides