[Serum levels of antioxidant enzymes, MDA and its relation to T-786C nitric oxide synthase gene polymorphism in patients with Coronary Artery Diseases [CAD]]

Background and Objectives: Various mutations on endothelial Nitric Oxide synthase [eNOs] gene can cause reduced production of NO and may accelerate the process of atherosclerosis. The study was designed to investigate the frequency of T-786C gene polymorphism in a group of patients suffering from CAD in North West of Iran, The association of the polymorphism with other risk factors including: Antioxidant activity indices, Lipid Peroxidation and MDA were also determined

Material and Methods: One hundred twenty subjects including 60 patients with angiographically diagnosed CAD and 60 age and sex matched normal control were studied. Exclusion criteria were; diabetes melitus smoking, preexisting malignancies and liver disease. The activities of SOD, GPX and TAC enzymes in the blood samples were determined in both groups using Randox Kits in an automated chemical analyzer [Abbott, model Alcyon 300,USA]. The concentration of MDA in the serum samples were assayed by spectrophotometerically using Thiobarbitoric acid reaction. The genotype studies were also carried out concomitantly using allele specific PCR

Results: Erythrocyte SOD, GPX activities and blood TAC level were reduced significantly in patients with CAD [P<0.05 in all cases]. The levels of serum MDA in CAD patients were significantly higher than controls [P<0.05]. The significantly higher frequency of eNOs -786C genotype was detected in CAD patients that indicate the significant association of C allels with CAD

Conclusion: The association between oxidative stress parameters and decrease Antioxidant activity may suggest their involvement in the pathogenesis of atherosclerosis. Increased frequency of T-786C polymorphism might be another risk factor in the progression of coronary artery disease

Evaluation of hs-CRP, antioxidant markers and MDA in patients of coronary artery disease [CAD] containing non-smokers and non-diabetics

Background: The oxidative stress and inflammation are cooperative events involved in atherosclerosis development. In the present study we assessed the association of MDA, antioxidant markers, high sensitive C - reactive protein [hs-CRP] and lipid status parameters in the patients with coronary artery disease [CAD]. Significant risk factors such as cigarette and diabet were excluded from the study. Methods: Oxidative stress parameters for example Malondialdehyde [MDA], antioxidant markers including: erythrocyte superoxide dismutase[SOD], Glutathion peroxidase [GPX], Total antioxidant capacity[TAC], The inflammation marker and serum lipid status parameters were measured in 120 subjects including 60 CAD patients with angiographically diagnosed CAD and 60 CAD-free subjects as a control group, also diabetics, smoking patients, patients with malignancy, renal and liver disease, and other disease were excluded from the study. Results: The serum MDA and hs-CRP levels were increased significantly as compared to controls. However, erythrocyte SOD, GPX activities and TAC level were reduced significantly in patients [P‹0.05 in all cases]. The levels of total cholesterol, Triglyceride, LDL-C were significantly higher and that of HDL-C was meaningfully lower than those of control [P‹0.05 in all cases]. Conclusion: The association between oxidative stress parameters, antioxidant markers, the inflammation index and lipid status parameters suggest their involvement in atherosclerosis development that may lead to CAD progression