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1.
West China Journal of Stomatology ; (6): 514-520, 2018.
Artículo en Chino | WPRIM | ID: wpr-772466

RESUMEN

OBJECTIVE@#This study aimed to explore the impacts of periodontitis on the visceral weight and weight percentage of obese animal models.@*METHODS@#A total of 64 C57BL/6J mice were divided into the following diet groups: high-fat diet (HFD) group (n=36), which was fed with high-fat diet to induce obesity, and low-fat diet (LFD) group (n=28), which was fed with low-fat diet as the control. After 16 weeks on diet, each diet group was divided into periodontitis (P) and control (C) groups. The P groups were induced for periodontitis by ligation with Porphyromonas gingivalis-adhered silk for 5 or 10 days, and the C groups were sham-ligated as the control. Visceral organs were resected and weighed. The organ weight percentage was calculated.@*RESULTS@#Compared with the LFD group, the HFD group significantly upregulated the weight and weight percentage of visceral adipose tissue and spleen (P<0.05), upregulated the weight of liver and kidney (P<0.05), and downregulated the weight percentage of liver and kidney (P<0.01). In the HFD group, the weight and weight percentage of spleen were downregulated in the P group (P<0.05), but were upregulated in the 10-day group compared with the 5-day group (P<0.05).@*CONCLUSIONS@#Periodontitis can affect the general morphology of the viscera (especially spleen) in obese animal models. Pathological indications in terms of immunometabolism might be present in the correlation between obesity and periodontitis.


Asunto(s)
Animales , Ratones , Dieta Alta en Grasa , Ratones Endogámicos C57BL , Ratones Obesos , Obesidad , Tamaño de los Órganos , Periodontitis
2.
Journal of Southern Medical University ; (12): 663-667, 2017.
Artículo en Chino | WPRIM | ID: wpr-273706

RESUMEN

<p><b>OBJECTIVE</b>To investigate the effect of periodontal therapy in controlling periodontitis and on insulin resistance and lipid metabolism in obese rats with periodontitis.</p><p><b>METHODS</b>Sprague-Dawley rats were randomized into normal group (group C), obese group (group O), periodontitis combined with obesity group (group P) and periodontal treatment group (group T). The obese rats in groups P and T were subjected to ligation of the maxillary second molar with silk thread to induce experimental periodontitis, and the rats in group T received periodontal therapy after the ligation. All the rats were sacrificed at the age of 24 weeks for measurement of blood lipids, insulin and blood glucose levels, and insulin resistance index (HOMA-IR) was calculated. The expressions of insulin receptor substrate-1 (IRS-1) and IRS-2 in the liver tissues were detected using real-time quantitative polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Compared with the obese rats in group O, the rats in group P showed significantly higher HOMA-IR and LDL-C and lower expressions of IRS-1 and IRS-2 mRNA expression and HDL-C level (P<0.05). Compared with those in group P, the mRNA expressions of IRS-1 and IRS-2 and HDL-C level were significantly increased and LDL-C level, TC level and HOMA-IR were all decreased in group T (P<0.05), but the level of TG was comparable between the two groups. Pathological examination revealed lessened inflammatory cell infiltration and tissue destruction in the upper jaw of the rats in group T; the rats in group P presented with the most obvious upper jaw destruction and steatosis and inflammatory cell infiltration in the liver.</p><p><b>CONCLUSION</b>Periodontal inflammation can downregulate the expression of IRS-1 and IRS-2 and increase insulin resistance and dyslipidemia in obese rats. Periodontal therapy produces a beneficial effect in improving insulin resistance and reducing dyslipidemia in obese rats.</p>

3.
Journal of Southern Medical University ; (12): 807-811, 2017.
Artículo en Chino | WPRIM | ID: wpr-360184

RESUMEN

<p><b>OBJECTIVE</b>To investigate the effects of non-surgical periodontal therapy on serum inflammatory factors and metabolism levels in obese rats with experimental periodontitis.</p><p><b>METHODS</b>Sixteen obese rats with experimental periodontitis were randomly divided into treatment group and control group with non-surgical periodontal therapy and no treatment, respectively. Oral glucose tolerance test was performed before treatment and 2 weeks after the treatment. All the rats were sacrificed 2 weeks after treatment and the orbital vein blood was taken to detect fasting blood glucose, fasting insulin, and serum level of C-reactive protein (CRP). Results Two weeks after periodontal treatment, fasting blood glucose (t=2.445, P=0.034) and beta cell function index (t=-2.543, P=0.027) were significantly lower in the treatment group than in the control group. Compared with those in the control group, CRP level (t=2.388, P=0.028) and the area under the curve in the oral glucose tolerance test (t=12.053, P=0.000) decreased significantly in the treatment group.</p><p><b>CONCLUSION</b>Non-surgical periodontal treatment can reduce serum CRP level and improve glucose metabolism in obese rats.</p>

4.
Chinese Journal of Applied Physiology ; (6): 247-250, 2013.
Artículo en Chino | WPRIM | ID: wpr-235388

RESUMEN

<p><b>OBJECTIVE</b>To investigate how transient low dose of hydroperoxide pretreatment prevents cardiac ischemia/reperfusion injury.</p><p><b>METHODS</b>SD rats were divided into 4 groups: sham operation (Sham), standard ischemia/reperfusion (I/R), ischemic preconditioning (IPC) and IR preceded by low H2O2 treatment. Cardiac function and injury parameter were compared among groups.</p><p><b>RESULTS</b>IPC protected reperfusion injury and improved cardiac function. Low H2O2 treatment played a role in cardioprotection similar to IPC. Low H2O2 was indeed generated in the early phase of simulated ischemia and attenuated cytochrome c release induced by high Ca2+ in isolated mitochondria.</p><p><b>CONCLUSION</b>Low H2O2 plays a critical role in cardioprotection probably by inhibiting mitochondrial permeability transition.</p>


Asunto(s)
Animales , Masculino , Ratas , Peróxido de Hidrógeno , Precondicionamiento Isquémico , Métodos , Ratas Sprague-Dawley , Daño por Reperfusión
5.
Chinese Journal of Stomatology ; (12): 141-145, 2010.
Artículo en Chino | WPRIM | ID: wpr-245240

RESUMEN

<p><b>OBJECTIVE</b>To investigate the prevalence of dentin hypersensitivity in Chinese urban adults aged between 20 - 69 years old and the factors related to dentin hypersensitivity.</p><p><b>METHODS</b>The Chinese national survey on dentin hypersensitivity was conducted in 20 - 69 years old adults in six representative cities, Beijing, Shanghai, Guangzhou, Wuhan, Chengdu, and Xi'an in 2008. A multi-stage stratified randomizing sampling method was used. Subjects were recruited from 36 urban survey sites in 6 cities. A structured questionnaire and a clinical examination on dentin hypersensitivity were used in the survey. The dentin hypersensitivity was diagnosed by a subject self-perceived short, sharp pain in response to a blast of cold air from a triple syringe administered to a tooth surface in 1 cm.</p><p><b>RESULTS</b>In total, 7939 twenty to sixty-nine years old subjects completed a structured interview and underwent a clinical examination on dentin hypersensitivity. Among them, 40.7% (3230/7939) of the subjects reported being suffered from teeth sensitivity. When confirmed using a blast of air from a triple syringe and by ruling out other causes of sensitivity, such as caries, the prevalence was 29.7% (2354/7939), and the mean number of sensitive teeth was 1.4. The highest prevalence of dentin hypersensitivity [39.1% (622/1592)] was found in 50 - 59 years old group. The commonest teeth affected were the premolar teeth and the commonest initiating factor was cold drinks. Female, low education level, with gingival recession, attachment loss, and with the history of acidic substances derived from the stomach was related to dentin hypersensitivity.</p><p><b>CONCLUSIONS</b>Dentin hypersensitivity was common in 20 - 69 years old Chinese urban adults. Dental professionals should give further emphasis to it.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Factores de Edad , Diente Premolar , China , Epidemiología , Sensibilidad de la Dentina , Epidemiología , Recesión Gingival , Pérdida de la Inserción Periodontal , Prevalencia , Factores de Riesgo , Encuestas y Cuestionarios , Población Urbana
6.
West China Journal of Stomatology ; (6): 185-189, 2010.
Artículo en Chino | WPRIM | ID: wpr-246626

RESUMEN

<p><b>OBJECTIVE</b>To explore the multi-differentiated capability of human periodontal ligament cell population (hPDLP), and provide a theoretical basis for the periodontal regeneration by tissue engineering technique.</p><p><b>METHODS</b>hPDLP was cultured from periodontium of human tooth by the outgrowth method. STRO-1 and CD 146 expression were investigated by flow cytometry. hPDLP was induced to odontogenic/osteogenic-like and adipogenic-like cell. The multilineage differentiation capacities of hPDLP were evaluated by alizarin red stain, oil red O stain, anti-CD146 and STRO-1 immunocytochemistry, and reverse-transcriptase polymerase chain reaction analysis.</p><p><b>RESULTS</b>hPDLP was isolated from human periodontium and most of the cells retained their fibroblastic spindle shape. hPDLP can be induced into osteoblast-like cells and adipocyte-like cells, and calcium deposition and lipid droplets were detected perspectively. And the eighth generation of hPDLP had weaker potential into adipocyte-like cells than the first passage, however, there was no difference to the aspect of calcification ability between the two passages.</p><p><b>CONCLUSION</b>hPDLP cultured in vitro can differentiate into adipocytes and osteoblasts, and the first to third passage cells may have the predominance of differentiation potential.</p>


Asunto(s)
Humanos , Adipocitos , Diferenciación Celular , Células Cultivadas , Fibroblastos , Citometría de Flujo , Técnicas In Vitro , Odontogénesis , Osteoblastos , Ligamento Periodontal , Regeneración , Ingeniería de Tejidos
7.
Journal of Southern Medical University ; (12): 433-436, 2009.
Artículo en Chino | WPRIM | ID: wpr-233770

RESUMEN

<p><b>OBJECTIVE</b>To construct an eukaryotic coexpression vector containing Mycobacterium tuberculosis heat shock protein 70 (mtHSP70) and enhanced green fluorescent protein (EGFP) controlled by cytomegalovirus promoter using pIRES-EGFP vector.</p><p><b>METHODS</b>The mtHSP70 gene fragment was amplified by PCR from pVAX-mtHSP70-HSV2gD using specific primers. The PCR product was cloned into the vector pMD 18-T vector, and the correct clone was selected according to DNA sequence analysis. The interested mtHSP70 gene fragment was subcloned into pCMV-IRES-EGFP vector with XhoI and EcoR I digestion. The recombinant plasmid was transfected into mouse melanoma B16 cell line, and the green fluorescent cells were detected by fluorescence microscopy and mtHSP70 expression was detected by Western blotting.</p><p><b>RESULTS</b>The recombinant plasmid obtained was confirmed by enzyme digestion. The transfected mouse melanoma B16 cells exhibited green fluorescence under fluorescence microscopy and expressed mtHSP70 protein as demonstrated by Western blotting.</p><p><b>CONCLUSION</b>The eukaryotic coexpression vector PCMV-mtHSP70-IRES-EGFP has been established to allow further investigation of the role of mtHSP70 gene in tumor immunotherapy.</p>


Asunto(s)
Animales , Ratones , Secuencia de Bases , Vacunas contra el Cáncer , Línea Celular Tumoral , Citomegalovirus , Genética , Metabolismo , Vectores Genéticos , Genética , Proteínas Fluorescentes Verdes , Genética , Proteínas HSP70 de Choque Térmico , Genética , Datos de Secuencia Molecular , Mycobacterium tuberculosis , Metabolismo , Proteínas Recombinantes de Fusión , Genética , Análisis de Secuencia de ADN
8.
Chinese Journal of Stomatology ; (12): 279-281, 2009.
Artículo en Chino | WPRIM | ID: wpr-346748

RESUMEN

<p><b>OBJECTIVE</b>To establish the expression and purification route for human amelogenin mature peptide in Escherichia coli and obtain the purified amelogenin (AMG) mature peptide.</p><p><b>METHODS</b>Recombined plasmid pGEX-4T-1-AMG was transformed to Escherichia coli BL21. After expression, AMG was purified with glutathione S-transferase fusion protein purification system (GSTrapFF) column.</p><p><b>RESULTS</b>Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting hybridization results showed that 45,000 GST-AMG fusing protein and 19,000 target AMG mature peptide were obtained successfully.</p><p><b>CONCLUSIONS</b>pGEX-4T-1-AMG-BL21 system is used successfully to express and purify human AMG mature peptide.</p>


Asunto(s)
Humanos , Amelogenina , Genética , Electroforesis en Gel de Poliacrilamida , Escherichia coli , Metabolismo , Glutatión Transferasa , Genética , Péptidos , Genética , Metabolismo , Proteínas Recombinantes de Fusión , Genética , Proteínas Recombinantes , Genética , Metabolismo
9.
Chinese Journal of Stomatology ; (12): 278-280, 2008.
Artículo en Chino | WPRIM | ID: wpr-235922

RESUMEN

<p><b>OBJECTIVE</b>To study the influence of lactational dioxin exposure (2, 3, 7, 8-tetrachlorodibenzo-p-dixon, TCDD) on development of alveolar bone in SD rat offspring.</p><p><b>METHODS</b>The rats of TCDD exposure group and control group were sacrificed and the alveolar bone with molars of PD60 rats in the two groups were embedded in resin. The sections were observed by fluorescent microscope. The alveolar bone formation was evaluated by histological examination, tetracycline fluorescence marker and quantitative histomorphometry. The indices of quantitative histomorphometry were compared.</p><p><b>RESULTS</b>The trabecular structure of alveolar bone was looser in TCDD exposure group than in the control group. The tetracycline fluorescence markers were more disorganized in TCDD group. The indices of quantitative histomorphometry of alveolar bone between two groups showed significantly different.</p><p><b>CONCLUSIONS</b>Lactational 2,3,7,8-TCDD exposure decreased the quality and quantity of alveolar bone in SD rat offspring. It is suggested that dioxins exposure may interrupt the spatial configuration.</p>


Asunto(s)
Animales , Femenino , Embarazo , Ratas , Lactancia , Exposición Materna , Dibenzodioxinas Policloradas , Toxicidad , Ratas Sprague-Dawley , Alveolo Dental , Patología
10.
West China Journal of Stomatology ; (6): 27-30, 2008.
Artículo en Chino | WPRIM | ID: wpr-296724

RESUMEN

<p><b>OBJECTIVE</b>To establish the expression and purification route for the gene encoding human amelongenin (AMG) mature peptide in Escherichia coli (E. coli).</p><p><b>METHODS</b>Recombined plasmid pGEX-4T-1/AMG was identified by double endonuclease digestion electrophoretogram and DNA sequence analysis. The recombined plasmid was transformed to E. coli BL21. The inducing time, isopropyl-beta-D-thiogalactopyranoside (IPTG) concentration and inducing temperature were optimized for the express system. Under the optimized condition, the target fusing protein in superatant, periplasm, plasm and inclusion body was analyzed separately. A great amount of target fusing protein was found in the dissoluble protein. AMG fusing protein was purified by the GSTrapFF affinity column.</p><p><b>RESULTS</b>Double endonuclease digestion electrophoretogram and DNA sequence analysis were done to identify the recombined vector pGEX-4T-1/AMG. The results were consistent with the anticipation. The optimum inducing time was 14.5 hours. The optimum IPTG concentration was 1.0 mmol/L. The optimum inducing temperature was 20 degrees C. Under this condition, the target protein was expressed to a maximum. Plentiful target protein was expressed in plasm and inclusion body under the optimized condition. A mount of plasm protein was obtained and purified by the GSTrapFF affinity column. The purified liquid was collected and analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PACE). The protein electrophoresis map showed that AMG fusing protein was purified successfully. After twice elution, high pure fusing protein was obtained.</p><p><b>CONCLUSION</b>pGEX-4T-1/AMG system is used successfully to express human AMG fusing protein.</p>


Asunto(s)
Humanos , Amelogenina , Escherichia coli , Vectores Genéticos
11.
Chinese Journal of Stomatology ; (12): 74-77, 2007.
Artículo en Chino | WPRIM | ID: wpr-292961

RESUMEN

<p><b>OBJECTIVE</b>To investigate the effect of inducible nitric oxide synthase (iNOS) on vascular endothelial growth factor (VEGF) expression.</p><p><b>METHODS</b>The vector containing short hairpin RNA of iNOS was transfected into Tca8113 cells using the RNA interference (RNAi) technique. The gene and protein expression of iNOS and VEGF was examined by RT-PCR and Western blot.</p><p><b>RESULTS</b>The expression of iNOS, VEGF gene in Tca8113 cells was significantly different between the experimental and control groups 24 h and 48 h after transfection (P < 0.05). The protein expression of iNOS was different between the two groups 36 h and 48 h after transfection, and of VEGF was also different between the two groups 48 h after transfection (P < 0.05).</p><p><b>CONCLUSIONS</b>The expression of VEGF could be down-regulated by silencing the iNOS gene in Tca8113 cells.</p>


Asunto(s)
Humanos , Carcinoma de Células Escamosas , Genética , Metabolismo , Línea Celular Tumoral , Regulación hacia Abajo , Regulación Neoplásica de la Expresión Génica , Óxido Nítrico Sintasa de Tipo II , Genética , Interferencia de ARN , Neoplasias de la Lengua , Genética , Metabolismo , Factor A de Crecimiento Endotelial Vascular , Genética , Metabolismo
12.
Chinese Journal of Plastic Surgery ; (6): 24-28, 2005.
Artículo en Chino | WPRIM | ID: wpr-255112

RESUMEN

<p><b>OBJECTIVE</b>To study the influence of experimentally osteoporosis to osteogenic efficiency of bone matrix gelatin(BMG) implanted into the calvarial defects of rats.</p><p><b>METHODS</b>Sixty-eight female SD rats of 12 +/- 1 weeks were randomly divided into two groups with 34 rats in each group. The ovaries were excised in the ovariectomized group (VG). The control group underwent sham surgery. Ninety days after ovariectomy, 10 rats from each group were examined to ensure the formation of postmenopausal osteoporosis by measuring bone density of the femur with single photon absorptiometric measurements. A critical-sized (8 mm in diameter) calvarial defect was created on the rest of 48 rats. Bone matrix gelatin was implanted to the defect. The rats were scarified at the 21st and 56th day after surgery respectively. The new bone forming capability of BMG was evaluated with undecalcified histological observation, tetracycline fluorescence marker, quantitative bone histomorphometry, At 90th day after ovariectomy, bone density of scanning electron microscopy and X-ray spectrometry.</p><p><b>RESULTS</b>OVG showed very significant difference compared with the control group (0.315 +/-.015) g/cm2 vs [(0.347 +/- 0.017) g/cm2, P < 0.01 ]. At the 21st day following the implantation operation, new bone formed within the bone defects in both groups. The amount of new bone in OVG was lower than the control group. The tetracycline-labeled region in the bone defect was sparser in the OVG. At the 56th day, the bone defects healed mostly in the control group but fibrous tissue filled parts of bone defect in the OVG. The distance between two fluorescent lines of incorporated tetracycline and the mean mineralization deposition were significantly lower in the OVG than the control at the 21st day and 56th day. Mineralization of callus in OVG was inferior. Significant difference was found between the OVG and the control group in the calcium to phosphate ratio of callus in bone defects at the two time-points.</p><p><b>CONCLUSIONS</b>Experimentally induced osteoporosis depressed osteogenic efficiency of BMG, suggesting that estrogen could play an important role in bone remodeling with bone substitute participating.</p>


Asunto(s)
Animales , Femenino , Ratas , Matriz Ósea , Química , Regeneración Ósea , Fémur , Metabolismo , Gelatina , Usos Terapéuticos , Osteogénesis , Osteoporosis , Cirugía General , Ovariectomía , Ratas Sprague-Dawley , Fracturas Craneales , Cirugía General
13.
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 27-30, 2005.
Artículo en Chino | WPRIM | ID: wpr-346596

RESUMEN

<p><b>OBJECTIVE</b>To investigate the effects of lead exposure at different levels in utero on the teeth eruption and enamel development of rat offsprings.</p><p><b>METHODS</b>27 pregnant SD rats were divided into three groups randomly: high level lead group (HLG), low level lead group (LLG) and control group with nine rats in each group. The three groups from the gestation day to the end of the gestation were given either deionized water in control group or deionized water containing 200 mg/L Pb2+ as lead acetate through drinking method in high level lead experimental group and 50 mg/L Pb2+ as lead acetate through drinking method in low level lead experimental group. The incisors of newborn rats were marked at the level of the gingival papilla on the 26th day after birth. On the 36th day, the incisors of newborn rats were marked again at the same level. Then the rat offsprings were killed and their blood was collected for lead analysis. The mandible incisors of rat offspring were separated and the content of Pb in incisors was determined by using a graphite furnace atomic absorption spectrometric method. The teeth of rat offspring were observed and the distance between two marks were measured by means of stereomicroscope. The ratio of calcium to phosphate of enamel of rat offspring was compared by electron probe microanalyses.</p><p><b>RESULTS</b>The level of blood lead in 200 mg/L, 50 mg/L treated rat offspring groups was higher than that in control group. The tooth lead of 200 mg/L, 50 mg/L treated rat offspring groups [(77.3 +/- 6.3), (27.8 +/- 4.5) microg/g] were higher than the control [(6.6 +/- 0.8) microg/g, P < 0.01]. Compared with the control group, the teeth of lead exposure experimental groups were smaller and severity of attrition was obvious and pulpal perforations were often observed. These appearances was more distinct in rats of high level lead experimental group. The incisors of lead-treated rat offspring erupted [(0.25 +/- 0.08), (0.30 +/- 0.09) mm/d] more slowly than control ones [(0.39 +/- 0.09) mm/d, P < 0.01]. The ratio of calcium to phosphate (Ca/P) decreased with the increase of lead exposure. It was found that Ca/P in lead exposure experimental groups (1.68 +/- 0.54), (1.37 +/- 0.47) was significantly lower than that in the control group (2.14 +/- 0.33).</p><p><b>CONCLUSION</b>Lead exposure in utero affects the normal eruption of teeth and enamel formation and the degree was related with the lead exposure level.</p>


Asunto(s)
Animales , Femenino , Embarazo , Ratas , Órgano del Esmalte , Plomo , Exposición Materna , Efectos Tardíos de la Exposición Prenatal , Patología , Ratas Sprague-Dawley , Erupción Dental
14.
West China Journal of Stomatology ; (6): 335-337, 2005.
Artículo en Chino | WPRIM | ID: wpr-300299

RESUMEN

<p><b>OBJECTIVE</b>Human soluble tumor necrosis factor receptor (sTNFR) can interfere with the biological functions of interleukin-1, which may be appropriate to the treatment of periodontitis. The eukaryote expression vector of the human sTNFR gene must be constructed prior to conducting transgene therapy of periodontitis.</p><p><b>METHODS</b>Both sTNFR gene and plasmid pcDNA 3.1 (+) DNA were digested with Kpn I and Xho I. After purification, the two fragments were ligated by TakaRa DNA Ligation Kit (Ver 2.0). This recombinant DNA was then transformed into E. Coli Competent Cells JM109 and positive clones were selected on the LB agarose plate containing ampicillin (80 microg/ul).</p><p><b>RESULTS</b>Six single clones were indentified by double digestion with kpn I and xho I and two fragments with the size of 5.4 kb and 1.0 kb were produced as expected.</p><p><b>CONCLUSION</b>The sTNFR gene was successfully inserted into the eukaryote expression vector plasmid pcDNA 3.1 (+) by recombination technology in vitro.</p>


Asunto(s)
Humanos , Escherichia coli , Eucariontes , Células Eucariotas , Vectores Genéticos , Plásmidos , Receptores del Factor de Necrosis Tumoral , Receptores Tipo I de Factores de Necrosis Tumoral , Factor de Necrosis Tumoral alfa
15.
Chinese Journal of Stomatology ; (12): 481-484, 2005.
Artículo en Chino | WPRIM | ID: wpr-303462

RESUMEN

<p><b>OBJECTIVE</b>To investigate the influence of a broad range of environmental conditions on initial rates of hydrogen peroxide produced by Streptococcus oralis (S. oralis).</p><p><b>METHODS</b>For each rate measurement, 1 ml aliquots of 10(12) cells/L mid-logarithmic phase S. oralis in TSBY were centrifuged and respectively washed by phosphate buffer containing 0.01-10 mmol/L glucose or sucrose, phosphate buffer with 5.0-7.5 pH or Bis-Tris buffer containing 0.01-100 mmol/L Ca(2+), 0.01-100 mmol/L F(-) or 0.01-100 mmol/L HFPO(3)(-). After S. oralis was cultured in respective buffer for 10, 20 and 30 min at 37 degrees C, the concentration of hydrogen peroxide in supernatant was assayed spectrophotometrically in 96-well micro-plate by ABTS-HRP at A(405).</p><p><b>RESULTS</b>Synthesis rate of hydrogen peroxide by S. oralis was 7.48 micromol/L per minute without carbohydrate, the synthesis rate of hydrogen peroxide by S. oralis increased with 0.01-10 mmol/L glucose and 0.01-10 mmol/L sucrose, but there was no statistically significant difference in synthesis rate among the carbohydrate groups. The rates of H2O2 synthesis were inhibited in the buffer at pH 5.0-6.0, compared with pH 7.0 (P < 0.05). Ca(2+) had little influence on the rate of H2O2 synthesis. IC(50) of H2O2 synthesis rates by S. oralis responded to FHPO(3)(-) and F(-) were 12.65 mmol/L and 1.90 mmol/L respectively.</p><p><b>CONCLUSIONS</b>Environmental conditions may influence the synthesis rate of H2O2 by S. oralis.</p>


Asunto(s)
Medios de Cultivo , Química , Glucosa , Peróxido de Hidrógeno , Metabolismo , Concentración de Iones de Hidrógeno , Metales Pesados , Streptococcus oralis , Metabolismo
16.
Chinese Journal of Stomatology ; (12): 50-53, 2005.
Artículo en Chino | WPRIM | ID: wpr-324109

RESUMEN

<p><b>OBJECTIVE</b>To investigate association of vitamin D receptor (VDR) gene polymorphisms with the susceptibility to chronic periodontitis (CP) of Han Nationality.</p><p><b>METHODS</b>Buccal swabs from 166 patients with severe, moderate and mild CP respectively and 80 matched control individuals were collected. DNA was extracted from these buccal swabs using Chelex-100 method. VDR BsmI, ApaI, TaqI were tested with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The distribution of the genotypes and allele frequencies in the patient and control groups were analyzed.</p><p><b>RESULTS</b>The frequency of VDR ApaI allele A was significantly higher among patients with CP than controls. Frequencies of VDR ApaI allele A were significantly higher in severe CP patients than in moderate CP and mild CP respectively. There was no significant difference in the genotype distribution or the allele frequencies of VDR BsmI and TaqI between the controls and CP patients.</p><p><b>CONCLUSIONS</b>These data indicate that VDR ApaI allele A may be related to the susceptibility to CP in Han Nationality.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Alelos , Pueblo Asiatico , Genética , Periodontitis Crónica , Genética , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Polimorfismo Genético , Receptores de Calcitriol , Genética
17.
West China Journal of Stomatology ; (6): 38-40, 2005.
Artículo en Chino | WPRIM | ID: wpr-329992

RESUMEN

<p><b>OBJECTIVE</b>To study the severity of periodontitis and risk factors in Chengdu.</p><p><b>METHODS</b>202 periodontitis patients (65 male, 137 female), aged from 25 to 60, were requested to fill a questionnaire. Probing depth (PD), clinical attachment level (CAL), gingival recession and bleeding on probing (BOP) on 6 sites of each tooth were measured and recorded.</p><p><b>RESULTS</b>The mean PD, AL, gingival recession and BOP% of 202 subjects was (3.2 +/- 0.31) mm, (3.5 +/- 0.37) mm, (0.3 +/- 0.02) mm and 21.16%. 59% of subjects missed at least one tooth. 129 subjects suffered with initial to moderate periodontitis. 73 subject suffered with advanced periodontitis. 40, 86, 55 and 21 subjects had received college education, high school education, middle school education and primary school education. 18% of subjects had smoking history, 67% subjects had tea/coffee history, 66% of subjects had psychosocial problem, and only 8% of subjects had received regular periodontal treatment. There is no relationship between the severity of periodontitis and education.</p><p><b>CONCLUSION</b>It is very important to develop an education program on oral healthy for people in Chengdu.</p>


Asunto(s)
Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Recesión Gingival , Pérdida de la Inserción Periodontal , Índice Periodontal , Periodontitis , Factores de Riesgo
18.
Chinese Journal of Stomatology ; (12): 146-148, 2004.
Artículo en Chino | WPRIM | ID: wpr-263434

RESUMEN

<p><b>OBJECTIVE</b>To investigate the relationship between Epstein-Barr virus (EBV) infection and chronic periodontitis.</p><p><b>METHODS</b>In 62 chronic periodontitis patients, subgingival plaque samples were collected from active periodontitis site, quiescent periodontitis site, and initial gingivitis sites of each subject. The nested polymerase chain reaction (nested-PCR) was used to determine the presence of EBV.</p><p><b>RESULTS</b>The EBV was detected in 58.0% of the samples from active periodontitis sites, which was significantly higher than that from quiescent periodontitis sites (58.0% vs 22.6%, P < 0.01) and from initial gingivitis sites (58.0% vs 19.4%, P < 0.01).</p><p><b>CONCLUSION</b>The results suggest that EBV infection is associated with the activity of chronic periodontitis.</p>


Asunto(s)
Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Enfermedad Crónica , Infecciones por Virus de Epstein-Barr , Herpesvirus Humano 4 , Periodontitis , Virología , Reacción en Cadena de la Polimerasa
19.
Chinese Journal of Stomatology ; (12): 185-188, 2004.
Artículo en Chino | WPRIM | ID: wpr-263420

RESUMEN

<p><b>OBJECTIVE</b>To detect the expression of recombinant plasmid PcDNA3.1-sTNFRI in vitro and evaluate the bioactivity of expressed sTNFRI.</p><p><b>METHODS</b>CHO cells were transfected with recombinant plasmid PcDNA3.1-sTNFRI by liposome. sTNFRI in cell culture supernatant was detected by ELISA and sTNFRI blockage of TNF-alpha cytotoxicity in L929 cells evaluated by MTT assay.</p><p><b>RESULTS</b>The expression of sTNFRI in transfected cell culture supernatant was higher than control groups (P < 0.001). The expressed sTNFRI could significantly neutralize TNF-alpha cytotoxicity in L929 cells.</p><p><b>CONCLUSIONS</b>These results showed that the recombinant plasmid PcDNA3.1-sTNFRI can be expressed in mammalian cells and the recombinant sTNFRI has biological function.</p>


Asunto(s)
Animales , Cricetinae , Humanos , Antígenos de Superficie , Células CHO , Clonación Molecular , ADN Complementario , Genética , Células Eucariotas , Metabolismo , Vectores Genéticos , Plásmidos , Genética , Receptores Tipo I de Factores de Necrosis Tumoral , Genética , Proteínas Recombinantes de Fusión , Genética , Farmacología , Transfección
20.
West China Journal of Stomatology ; (6): 158-161, 2004.
Artículo en Chino | WPRIM | ID: wpr-319030

RESUMEN

<p><b>OBJECTIVE</b>To investigate the association between Fc gamma receptor IIA gene polymorphism and susceptibility to chronic periodontitis in Chinese Han nationality.</p><p><b>METHODS</b>DNA samples were collected with buccal swabs from 63 patients with severe chronic periodontitis(CP), 103 patients with mild to moderate CP and 80 healthy individuals as control. Polymorphism in Fc gamma receptor IIA gene cluster was analyzed with PCR-SSP. The genotype distribution and allele frequency among different groups were compared.</p><p><b>RESULTS</b>It was found that the frequency of Fc gamma RIIA-R/R131 genotype was significantly higher in patients with severe CP (19.05%) compared to that of the healthy controls (P < 0.0125).</p><p><b>CONCLUSION</b>The Fc gamma RIIA-R/R131 genotype may be one of the contributors for the increased susceptibility to severe CP in Chinese Han nationality.</p>


Asunto(s)
Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Antígenos CD , Genética , China , Etnología , Enfermedad Crónica , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Genética , Genotipo , Periodontitis , Genética , Reacción en Cadena de la Polimerasa , Métodos , Polimorfismo Genético , Receptores de IgG , Genética
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