RESUMEN
Splenic B-cell lymphomas (SBCLs) show characteristically pronounced splenomegaly without significant lymphadenopathy. Distinguishing hairy cell leukemia (HCL) from other SBCLs (splenic marginal zone lymphoma [SMZL], variant HCL [v-HCL], and splenic diffuse red pulp small B-cell lymphoma [SDRPL]) is essential to determine suitable treatments and prognoses. With advances in diagnostic modalities and therapies, splenectomy is not commonly performed, and thus diagnosis of HCL must be based on the results obtained using blood and bone marrow samples. Annexin A1 is known as the most specific marker for HCL. There has yet been no report of the assessment of annexin A1 immunostaining from Korea. In this study we analyzed samples from 13 Korean patients with SBCLs (three HCL, three v-HCL, six SMZL, and one SDRPL) from May 2001 to December 2016. Immunohistochemical analyses for annexin A1 and CD20 were performed using bone marrow sections; molecular analyses for detection of the BRAF V600E mutation were also performed. All HCL patients showed positive results for annexin A1 immunostaining and the presence of the BRAF V600E mutation, and negative results for other SBCLs. Our results confirmed the high specificity of annexin A1 and the BRAF V600E mutation as HCL markers. Molecular analysis requires expensive equipment and substantial manpower. Annexin A1 is a better alternative as an HCL marker than the BRAF V600E mutation in terms of cost-effectiveness.
Asunto(s)
Humanos , Anexina A1 , Médula Ósea , Diagnóstico , Corea (Geográfico) , Leucemia de Células Pilosas , Enfermedades Linfáticas , Linfoma , Linfoma de Células B , Pronóstico , Sensibilidad y Especificidad , Esplenectomía , EsplenomegaliaRESUMEN
High oxygen-affinity hemoglobin (Hb) variants and a 2,3-diphosphoglycerate (2,3-DPG) deficiency could cause congenital (familial) erythrocytosis. High oxygen-affinity Hb variants and a 2,3-DPG deficiency might result in low tissue oxygen tension left-shifted oxygen dissociation curves and reduction in the standard P₅₀ value (P(50,std), oxygen tension at which haemoglobin is 50% saturated). Hence, the P(50,std) value is considered while formulating diagnostic strategies for erythrocytosis. In this study, we established a reference range for P(50,std) using an International Federation of Clinical Chemistry and Laboratory Medicine-approved equation (Hill's equation) for individual single venous/arterial blood samples. Blood gas analysis results of 243 samples with oxygen saturation ranging from 40%–90% (Hb < 16 mg/dL) were selected. The reference range of P(50,std) was in the 2.5th–97.5th percentile, and was 25.9–27.3 mm Hg. Hill's equation is a simple approved method for evaluating the P(50,std) values. Only a single sample of venous or arterial blood and a blood gas analyser are required to obtain the P(50,std). Our study provides a useful tool for the diagnostic work-up of patients with erythrocytosis.
Asunto(s)
Humanos , 2,3-Difosfoglicerato , Análisis de los Gases de la Sangre , Química Clínica , Métodos , Oxígeno , Policitemia , Valores de ReferenciaRESUMEN
BACKGROUND: Acute alcoholic intoxication patients (AAIP) are a common public health problem. The aim of this study was to perform a comprehensive laboratory analysis for these patients to investigate the co-morbid medical problem. METHODS: We retrospectively reviewed laboratory findings of AAIP who were transferred to the emergency department (ED) from January 2017 to June 2017. RESULTS: A total of 160 male patients were enrolled. Sixteen patients (16/160, 10.0%) and three patients (3/160, 1.9%) had macrocytic anemia and microcytic anemia, respectively. A total of 33 patients (33/160, 20.6%) showed thrombocytopenia ( 7.0%). Positive rates of hepatitis B surface antigen and anti-HBs antibody (anti-HBs Ab) were 3.5% (5/141) and 49.0% (68/141), respectively. CONCLUSION: Patients with AAIP who were transferred to ED had various laboratory abnormalities (anemia, thrombocytopenia, high HbA1c). They had low positive rate of anti-HBs Ab. This might be a public health problem, suggesting the need of hepatitis B virus vaccination program for AAIP. Our data suggest the need of further nationwide studies.
Asunto(s)
Humanos , Masculino , Intoxicación Alcohólica , Alcohólicos , Anemia , Anemia Macrocítica , Enfermedad Crónica , Servicio de Urgencia en Hospital , Tasa de Filtración Glomerular , Antígenos de Superficie de la Hepatitis B , Virus de la Hepatitis B , Hepatitis B , Hepatitis , Corea (Geográfico) , Salud Pública , Insuficiencia Renal Crónica , Estudios Retrospectivos , Albúmina Sérica , Trombocitopenia , VacunaciónRESUMEN
BACKGROUND: In general, internal/external quality control of special stains for diagnosis of hematological diseases may be unavailable in a clinical laboratory owing to the lack of an appropriate positive/negative control material. METHODS: We developed a protocol on positive/negative control materials for five special stains (iron, myeloperoxidase [MPO], periodic acid-Schiff [PAS], Sudan black B [SBB], and alpha-naphthyl acetate esterase [ANAE]) using a hematological malignant cell line. First, we compared stainability of seven cell lines (HL-60, THP-1, K562, Kasumi-1, KG-1, KO52, and NKM-1), then confirmed duration of stable stainability. A proficiency test using external quality control materials was conducted at eleven institutions, which participated voluntarily. RESULTS: HL-60 and THP-1 cell lines, which showed good stainability among the seven cancer cell lines, were selected as external quality control materials. The stainability of a prepared cell line fixed on control slides was stable for 3–4 weeks (MPO, SBB, and PAS) or 9–10 weeks (ANAE). The stainability of paraffin-embedded control material for iron stain was stable for 3 months. The results from 11 institutions were the same on iron, MPO, SBB, and ANAE. Nevertheless, two of 10 institutes showed discrepant results on PAS. CONCLUSIONS: In this study, we demonstrated that cell lines could serve as a standard quality control material for special stains. Most institutions showed representative results on special stains except for PAS. This protocol for special stain may be useful as an external or internal quality control in a haematology laboratory.
Asunto(s)
Academias e Institutos , Línea Celular , Colorantes , Diagnóstico , Enfermedades Hematológicas , Hematología , Hierro , Ensayos de Aptitud de Laboratorios , Naftol AS D Esterasa , Peroxidasa , Control de Calidad , SudánRESUMEN
Congenital erythrocytosis (CE) is a rare and heterogeneous disease. The high oxygen affinity hemoglobin (Hb) variants are the most common cause of CE. Herein, we report a Korean patient with isolated erythrocytosis. A 25-year-old man was referred to our hospital for evaluation of high Hb level (Hb 20.4 g/dL, hematocrit 58%, reticulocyte count 2.90%, white blood cell count 6.83×10⁹/L, and platelet count 195×10⁹/L). Bone marrow biopsy revealed normocellular marrow without myeloproliferative features. JAK2 (V617F, exon 12), CALR (exon 9), and MPL W515K/L mutations were not detected. P₅₀ (partial pressure at which Hb is half saturated with oxygen), which is an indicator of left-shift of oxygen dissociation curve (high oxygen affinity state), was 14.3 mm Hg (reference value 22.6–29.4 mm Hg). He was suspected to have CE. Mutation analysis of the HBB gene revealed the known Hb variant, Hb Heathrow [β103(G5)Phe→Leu]. This is the first report of Hb Heathrow in Asian.
Asunto(s)
Adulto , Humanos , Pueblo Asiatico , Biopsia , Médula Ósea , Exones , Hematócrito , Recuento de Leucocitos , Oxígeno , Recuento de Plaquetas , Policitemia , Recuento de ReticulocitosRESUMEN
BACKGROUND: There are few commercial quality-control (QC) materials for internal QC of flow cytometric analysis, especially for leukemia/lymphoma immunophenotyping. The purpose of this study was to investigate the current QC status of flow cytometry in Korea through a questionnaire survey, and develop new QC materials using cultured cell lines for markers which QC materials are unavailable. METHODS: The current state of internal QC of flow cytometry in Korea was investigated via a questionnaire survey. Cell lines to be used as QC materials were cultured and produced as QC materials. Cell viability and the expression of markers on the cultured cell lines were tested by flow cytometry to confirm the stability of the QC materials. Simulated quality assessment results for the cultured cell line QC materials were sent to laboratories for external proficiency testing (PT). RESULTS: Seventeen medical institutions completed the questionnaire survey. Hematopoietic stem cell count (CD34) and lymphocyte subset panel items in most of these institutions were managed using commercialized QC materials. The markers that could not be managed by QC materials were CD117, MPO (myeloperoxidase), TdT (terminal deoxynucleotidyl transferase), CD20, CD10, CD64, CD79α, FMC7, cytoCD22, CD23, CD34, and CD61. Five cell lines expressing these markers were selected and sent as QC materials. PT results for most of the markers were in concordance, except those for FMC7 and CD64. CONCLUSIONS: For the QC control of flow cytometry without commercialized QC materials, cultured cell lines are useful and can be used as an alternative for management of reagents used in flow cytometric analysis.
Asunto(s)
Línea Celular , Supervivencia Celular , Células Cultivadas , Citometría de Flujo , Células Madre Hematopoyéticas , Inmunofenotipificación , Indicadores y Reactivos , Corea (Geográfico) , Ensayos de Aptitud de Laboratorios , Subgrupos Linfocitarios , Control de CalidadRESUMEN
BACKGROUND: The clinical usefulness of flow cytometry (FCM) for the diagnosis of leptomeningeal diseases (LMD) in non-Hodgkin lymphomas has been suggested in previous studies but needs to be further validated. With this regards, we evaluated the use of FCM for LMD in a series of Korean patients with non-Hodgkin lymphoma. METHODS: FCM and cytomorphology were conducted using samples obtained from clinically suspected LMD patients, follow-up LMD patients, and those with high risk of developing tumorigenic diseases. We then compared results of FCM and cytomorphology. In total, 55 and 47 CSF samples were analyzed by FCM and cytomorphology, respectively. RESULTS: Of the samples analyzed, 25.5% (14/55) and 12.8% (6/47) were positive by FCM and cytomorphology, respectively. No samples were determined as negative by FCM but positive by cytomorphology. Seven patients were positive only by FCM and negative by cytomorphology, and six among them were clinically confirmed to have LMD either by follow-up cytomorphology or imaging study. CONCLUSIONS: We observed a high detection rate of tumor cells by FCM compared with cytomorphology. FCM study can be useful in early sensitive detection of LMD.
Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Citometría de Flujo , Glucosa/líquido cefalorraquídeo , Leucocitos/citología , Linfoma de Células B Grandes Difuso/diagnóstico , Linfoma no Hodgkin/complicaciones , Neoplasias Meníngeas/líquido cefalorraquídeo , Pronóstico , Estudios Retrospectivos , Tasa de SupervivenciaRESUMEN
No abstract available.
Asunto(s)
Preescolar , Femenino , Humanos , Citometría de Flujo , Hidroa Vacciniforme/diagnóstico , Inmunofenotipificación , Linfocitosis/complicaciones , Linfoma/diagnóstico , Receptores de Antígenos de Linfocitos T gamma-delta/genética , Factor de Transcripción STAT3/genética , Análisis de Secuencia de ADN , Piel/metabolismo , Linfocitos T/metabolismoRESUMEN
The causes of cytopenia in patients with severe fever with thrombocytopenia syndrome (SFTS) are not fully understood until now. We reviewed the bone marrow (BM) findings of patients with SFTS to unravel the cause of the cytopenia. Three Korean SFTS were enrolled in this study. Thrombocytopenia, neutropenia, and anemia were detected in all three patients. Severe hypocellular marrow (overall cellularity <5%) and a decreased number of megakaryocytes were noted in one patient, and hypo-/normocellular marrow and an increased number of hemophagocytic histiocytes were observed in two patients. Megakaryocytes were relatively preserved in two patients. Although a limited number of cases are available, our observations suggest that both BM suppression and peripheral destruction or sequestration are causes of cytopenia of patients with SFTS. To the best of our knowledge, this is the first well documented pathologic evaluation of Korean SFTS.
Asunto(s)
Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Médula Ósea/patología , Fiebre/complicaciones , Histiocitos/patología , Neutropenia/complicaciones , Pancitopenia/complicaciones , Síndrome , Trombocitopenia/complicacionesRESUMEN
BACKGROUND: Recent studies have identified a high prevalence of the MYD88 L265P mutation in lymphoplasmacytic lymphoma (LPL)/Waldenstrom macroglobulinemia (WM) cases, whereas low frequencies have been observed in other B cell non-Hodgkin lymphomas (NHLs). METHODS: We evaluated the sensitivity of the mutant enrichment 3'-modified oligonucleotide (MEMO)-PCR technique, a new detection method. We examined the MYD88 L265P mutation in a series of Korean patients with LPL/WM and other B cell NHLs in bone marrow aspirates, using the MEMO-PCR technique. RESULTS: The sensitivity of MEMO-PCR was estimated to be approximately 10-16.7%. MYD88 L265P was detected in 21 of 28 LPL cases (75%) and only three of 69 B cell NHL cases (4.3%). CONCLUSION: Although MEMO-PCR had relatively low sensitivity, we confirmed the high prevalence of the MYD88 L265P mutation in Korean LPL patients. Our study suggests the diagnostic value of MYD88 L265P for differentiating B-cell NHLs.
Asunto(s)
Humanos , Linfocitos B , Médula Ósea , Linfoma , Linfoma no Hodgkin , Métodos , Prevalencia , Macroglobulinemia de WaldenströmRESUMEN
No abstract available.
Asunto(s)
Femenino , Humanos , Adulto Joven , Análisis Mutacional de ADN , Mutación del Sistema de Lectura , Factor de Transcripción GATA2/genética , Predisposición Genética a la Enfermedad , Pérdida Auditiva Sensorineural/diagnóstico , Linfedema/diagnóstico , Síndromes Mielodisplásicos/diagnóstico , Fenotipo , República de CoreaRESUMEN
No abstract available.
Asunto(s)
Humanos , Lactante , Masculino , Médula Ósea/metabolismo , Análisis Mutacional de ADN , Reordenamiento Génico de Linfocito T , Inmunofenotipificación , Linfohistiocitosis Hemofagocítica/diagnóstico , Proteínas de la Membrana/química , Polimorfismo Conformacional Retorcido-Simple , República de Corea , Linfocitos T/inmunologíaRESUMEN
No abstract available.
Asunto(s)
Humanos , Masculino , Antineoplásicos/uso terapéutico , Secuencia de Bases , ADN/química , Análisis Mutacional de ADN , Proteínas de Fusión bcr-abl/genética , Genotipo , Mesilato de Imatinib/uso terapéutico , Cariotipificación , Reacción en Cadena de la Polimerasa Multiplex , Mutación , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnósticoRESUMEN
Quality control for genetic tests has become more important as testing volume and clinical demands have increased dramatically. The diagnostic genetics subcommittee of Korean Association of External Quality Assessment Service conducted two trials in 2014 based on cytogenetics and molecular genetics surveys. A total of 44 laboratories participated in the chromosome surveys, 33 laboratories participated in the fl uorescence in situ hybridization (FISH) surveys, and 130 laboratories participated in the molecular genetics surveys as a part of these trials. All laboratories showed acceptable results in the chromosome and FISH surveys. The molecular genetics surveys included various tests: Mycobacterium tuberculosis detection, hepatitis B and C virus detection and quantification, human papilloma virus genotyping, gene rearrangement tests for leukaemia and lymphomas, genetic tests for JAK2, FMS-like tyrosine kinase 3, nucleophosmin, cancer-associated genes (KRAS, EGFR, KIT, and BRAF), hereditary breast and ovarian cancer genes (BRCA1 and BRCA2), Li-Fraumeni syndrome (TP53), Wilson disease (ATP7B), achondroplasia (FGFR3), Huntington disease, spinocerebellar ataxia, spinal and bulbar muscular atrophy, mitochondrial encephalopathy with lactic acidosis and stroke like episodes, myoclonic epilepsy ragged red fibre, Prader-Willi/Angelman syndrome, Duchenne muscular dystrophy, spinal muscular atrophy, fragile X syndrome, nonsyndromic hearing loss and deafness (GJB2), multiple endocrine neoplasia 2 (RET), Leber hereditary optic neuropathy (major mutation), apolipoprotein E genotyping, methylenetetrahydrofolate reductase genotyping, ABO genotyping, and DNA sequencing analysis. Molecular genetic surveys showed excellent results for most of the participants. The external quality assessment program for genetic analysis in 2014 proved to be helpful for continuous education and the evaluation of quality improvement.
Asunto(s)
Humanos , Acondroplasia , Acidosis Láctica , Apolipoproteínas , Mama , Citogenética , Sordera , Educación , Epilepsias Mioclónicas , Tirosina Quinasa 3 Similar a fms , Síndrome del Cromosoma X Frágil , Reordenamiento Génico , Genética , Pérdida Auditiva , Hepatitis B , Degeneración Hepatolenticular , Enfermedad de Huntington , Hibridación in Situ , Corea (Geográfico) , Síndrome de Li-Fraumeni , Linfoma , Metilenotetrahidrofolato Reductasa (NADPH2) , Biología Molecular , Técnicas de Diagnóstico Molecular , Neoplasia Endocrina Múltiple , Atrofia Muscular Espinal , Trastornos Musculares Atróficos , Distrofia Muscular de Duchenne , Mycobacterium tuberculosis , Atrofia Óptica Hereditaria de Leber , Neoplasias Ováricas , Papiloma , Garantía de la Calidad de Atención de Salud , Control de Calidad , Mejoramiento de la Calidad , Análisis de Secuencia de ADN , Ataxias Espinocerebelosas , Accidente CerebrovascularRESUMEN
No abstract available.
Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Embarazo , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Ciclofosfamida/uso terapéutico , Doxorrubicina/uso terapéutico , Región Variable de Inmunoglobulina/genética , Leucemia de Células Pilosas/tratamiento farmacológico , Linfoma no Hodgkin/tratamiento farmacológico , MAP Quinasa Quinasa 1/genética , Mutación , Polimorfismo de Nucleótido Simple , Prednisona/uso terapéutico , Proteínas Proto-Oncogénicas B-raf/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Vincristina/uso terapéuticoRESUMEN
Protein S (PS), a vitamin K-dependent glycoprotein, performs an important role in the anticoagulation cascade as a cofactor of protein C. Because of the presence of a pseudogene and two different forms of PS in the plasma, protein S deficiency (PSD) is one of the most difficult thrombophilias to study and a rare blood disorder associated with an increased risk of thrombosis. We describe a unusual case of previously healthy 37-year-old man diagnosed with portal-splenic-mesenteric vein thrombosis secondary to PSD. The patient was admitted to the hospital due to continuous nonspecific abdominal pain and nausea. Abdominal computed tomography revealed acute venous thrombosis from inferior mesenteric vein to left portal vein via splenic vein, and laboratory test revealed decreased PS antigen level and PS functional activity. Conventional polymerase chain reaction and direct DNA sequencing analysis of the PROS1 gene demonstrated duplication of the 166th base in exon 2 resulting in frame-shift mutation (p.Arg56Lysfs*10) which is the first description of the new PROS1 gene mutation to our knowledge. Results from other studies suggest that the inherited PSD due to a PROS1 gene mutation may cause venous thrombosis in a healthy young man without any known predisposing factor.
Asunto(s)
Adulto , Humanos , Masculino , Anticoagulantes/uso terapéutico , Secuencia de Bases , Proteínas Sanguíneas/genética , Codón de Terminación , Exones , Venas Mesentéricas/diagnóstico por imagen , Polimorfismo de Longitud del Fragmento de Restricción , Vena Porta/diagnóstico por imagen , Deficiencia de Proteína S/complicaciones , Análisis de Secuencia de ADN , Vena Esplénica/diagnóstico por imagen , Tomografía Computarizada por Rayos X , Trombosis de la Vena/diagnósticoRESUMEN
No abstract available.
Asunto(s)
Adulto , Femenino , Humanos , Embarazo , Secuencia de Bases , Médula Ósea/patología , Cromosomas Humanos Par 12 , Cromosomas Humanos Par 9 , Hibridación Fluorescente in Situ , Datos de Secuencia Molecular , Proteínas de Fusión Oncogénica/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Proteínas Tirosina Quinasas/genética , Análisis de Secuencia de ADN , Translocación GenéticaRESUMEN
Quality control for genetic tests has become more important as the test volume and clinical demands increase dramatically. The diagnostic genetics subcommittee of the Korean Association of Quality Assurance for Clinical Laboratories performed two trials for cytogenetics and molecular genetics surveys in 2013. A total of 43 laboratories participated in the cytogenetic surveys, 30 laboratories participated in the fluorescent in situ hybridization surveys, and 122 laboratories participated in the molecular genetics surveys in 2013. Almost all of them showed acceptable results. However, some laboratories had unacceptable results for karyotype nomenclature, detection of complex cytogenetic abnormalities in hematologic neoplasms and constitutional anomalies. The molecular genetics surveys included various tests: Mycobacterium tuberculosis detection, hepatitis B and C virus detection and quantification, human papilloma virus genotyping, gene rearrangement tests for leukaemia and lymphomas, genetic tests for JAK2, fms-related tyrosine kinase 3, Nucleophosmin, cancer-associated genes (KRAS, EGFR and BRAF), hereditary breast and ovarian cancer genes (BRCA1 and BRCA2), Li-Fraumeni syndrome (TP53), Wilson disease (ATP7B), achondroplasia (FGFR3), Huntington disease, spinocerebellar ataxia, spinal and bulbar muscular atrophy, mitochondrial encephalopathy with lactic acidosis and stroke like episodes, myoclonic epilepsy associated with ragged-red fibers, Prader-Willi/Angelman syndrome, Duchenne muscular dystrophy, spinal muscular atrophy, Fragile X syndrome, non-syndromic hearing loss and deafness (GJB2), apolipoprotein E genotyping, methylenetetrahydrofolate reductase genotyping, ABO genotyping and DNA sequence analysis. Molecular genetic surveys showed excellent results for most of the participants. The external quality assessment program for genetic analysis in 2013 was proved to be helpful for continuous education and evaluation of quality improvement.
Asunto(s)
Humanos , Acondroplasia , Acidosis Láctica , Apolipoproteínas , Mama , Aberraciones Cromosómicas , Citogenética , Sordera , Educación , Síndrome del Cromosoma X Frágil , Reordenamiento Génico , Genética , Pérdida Auditiva , Neoplasias Hematológicas , Hepatitis B , Degeneración Hepatolenticular , Enfermedad de Huntington , Hibridación Fluorescente in Situ , Cariotipo , Corea (Geográfico) , Síndrome de Li-Fraumeni , Linfoma , Síndrome MERRF , Metilenotetrahidrofolato Reductasa (NADPH2) , Biología Molecular , Atrofia Muscular Espinal , Trastornos Musculares Atróficos , Distrofia Muscular de Duchenne , Mycobacterium tuberculosis , Neoplasias Ováricas , Papiloma , Proteínas Tirosina Quinasas , Control de Calidad , Mejoramiento de la Calidad , Análisis de Secuencia de ADN , Ataxias Espinocerebelosas , Accidente CerebrovascularRESUMEN
BACKGROUND: In support of safe and appropriate utilization of blood products, the Korean Ministry of Health and Welfare and the Korean Society of Blood Transfusion developed transfusion guidelines in 2009. We evaluated the appropriateness of blood transfusions on the basis of these proposed guidelines. METHODS: We investigated the awareness of the 2009 proposed guidelines and the transfusion guidelines currently in use through a questionnaire administered to the physicians of a tertiary care hospital. We provided the 2009 proposed transfusion guidelines through summarized pop-ups for each blood product that appeared in the hospital information system whenever a physician ordered blood products, and promoted the guidelines by posting it on the bulletin board and the hospital information system for one month. Evaluation of the appropriateness of blood transfusion was conducted by reviewing the medical records of patients who were transfused within one month before and one month after the promotion. Further, we also examined the rates of blood wastage and return. RESULTS: Rates of appropriately transfused blood products changed from 29.4% to 33.1% for red blood cells, from 16.9% to 68.9% for platelets, and from 54.8% to 33.3% for fresh frozen plasma. The decreased appropriate transfusion rate of fresh frozen plasma might be due to the small number of transfusions performed during the short study period. The rates of blood wastage and return decreased from 1.77% to 1.21% and from 3.91% to 3.00%, respectively. CONCLUSIONS: Promotion of the new transfusion guidelines lowered the incidence of inappropriate transfusions. Continuous promotion and establishment of these guidelines after adjustments according to the status of each hospital are necessary.