RESUMEN
<p><b>OBJECTIVE</b>To investigate the effect of antibody-targeted chemotherapy against human prostate cancer LNCaP cells in vitro.</p><p><b>METHODS</b>The monoclonal antibody 7E11C5.3 against human prostate cancer was conjugated to pingyangmycin (PYM), mediated by dextran T-40, and the immunoreactivity of 7E11C5.3 was determined by indirect enzyme-linked immunosorbent assay. The bacteriostatic activity of the conjugate was determined using TTC assay, and its cytotoxicity against LNCaP cells was determined by MTT assay.</p><p><b>RESULTS</b>The 7E11C5.3:PYM molar ratio was l:54 in the conjugate, and the immunoreactivity of 7E11C5.3 was decreased by approximately 10% to 20% after conjugation. The bacteriostatic activity of conjugated PYM was 25% of that of free PYM. The 50% inhibitory doses (IC50) of 7E11C5.3-PYM conjugate and free PYM against the in vitro cultured LNCaP cells were 9.41-/+1.98 microg/ml and 29.92-/+7.88 microg/ml, respectively.</p><p><b>CONCLUSION</b>7E11C5.3-PYM conjugate displays stronger cytotoxicity against anti-prostate cancer effects than free PYM.</p>
Asunto(s)
Humanos , Masculino , Antibióticos Antineoplásicos , Farmacología , Anticuerpos Monoclonales , Farmacología , Bleomicina , Farmacología , Línea Celular Tumoral , Supervivencia Celular , Citotoxicidad Inmunológica , Alergia e Inmunología , Sistemas de Liberación de Medicamentos , Inmunoconjugados , Farmacología , Neoplasias de la Próstata , Alergia e Inmunología , PatologíaRESUMEN
<p><b>OBJECTIVE</b>To evaluate the influence of Chinese drug Dahuang Zhechong pill on the hepatocellular function.</p><p><b>METHOD</b>Thirty-seven patients with hepatocirrhosis and twelve normal controls were performed the hepatobiliary scintgraphy with Tc-99m labeled ethylene hepatobiliary iminodiacetic acid (99 mTc-EHIDA), and the biochemical examination of hepatic function. There was 19 cases repeated the imaging after 6 months treated with chineses drug. By the three compartmental model configurations, the function parameters of hepatocellular extraction and excretion were calculated.</p><p><b>RESULT</b>In the hepatocirrhosis groups, the hepatocellular uptake peak time and mean residence index were higher than those in normal controls (P < 0.01). Compared to normal controls, the uptake index, uptake speed index and descendent speed index were decreased markedly (P < 0.05). After treatment for 6 months with Chinese drug, the level of serum transaminase, globulin and bilirubin was lower than that before treatment. The uptake peak time and mean residence index decreased notably after treatment for 6 months (P < 0.01), and the uptake index increased, (P < 0.05).</p><p><b>CONCLUSION</b>Chinese drug Dahuang Zhechong pill may improve the hepatocellular function and liver function status in patients with hepatocirrhosis.</p>
Asunto(s)
Adulto , Humanos , Masculino , Persona de Mediana Edad , Bilirrubina , Sangre , Medicamentos Herbarios Chinos , Farmacología , Globulinas , Metabolismo , Hepatocitos , Metabolismo , Cirrosis Hepática , Sangre , Quimioterapia , Metabolismo , Pruebas de Función Hepática , Transaminasas , SangreRESUMEN
<p><b>OBJECTIVE</b>To investigate the HLA-DRB1 allele polymorphism in Fujian Han nationality population.</p><p><b>METHODS</b>Six hundred and twenty individual samples collected from unrelated Fujian Han population were subjected to genotyping using oligonucleotide microarray technique. And the allele frequencies of HLA-DRB1 were calculated and compared with other populations.</p><p><b>RESULTS</b>Fourteen HLA-DRB1 alleles of Fujian Han population were detected. The gene frequencies ordered from high to low were HLA-DRB1*9, 12, 15, 4, 8 respectively.</p><p><b>CONCLUSION</b>The HLA-DRB1 distribution of Fujian Han population shares some genetic characters with southern Chinese Han populations, but these characters differ from northern Chinese populations.</p>
Asunto(s)
Humanos , Alelos , Pueblo Asiatico , Genética , China , Etnología , Etnicidad , Frecuencia de los Genes , Genética , Genética de Población , Geografía , Antígenos HLA-DR , Genética , Cadenas HLA-DRB1 , Polimorfismo Genético , Grupos de PoblaciónRESUMEN
Objective To investigate the renal function changes of the children with breast milk jaundice(BMJ) and effect of early interference treatment on renal function. Methods Serum bilirubin and urine - minim protein (?2-MG,?1-MG, Alb and IgG) of the 50 patients with BMJ were measured when they were in hospital within 12 hours and the last day separately , at the same time, 20 healthy newborns had been chosen to serve as control group. Results Compared with control group, the urine minim protein of treatment group increased with the rise of serum bilirubin. When serum bilirubin was 205.2 - 256.5 ?mol/L, urine ?2- MG had mild increasing (P