Morphological characteristics of Plasmodium yoelii schizonts in ghost erythrocytes / 中国医学科学院学报

<p><b>OBJECTIVE</b>To observe the morphological characteristics of Plasmodium yoelii schizogony in their ghost erythrocytes.</p><p><b>METHODS</b>Saponify, hypotonic shock, and electron microscopy were used to observe the different fashions of erythrocytic parasites and their characteristic organellae in ghost erythrocytes.</p><p><b>RESULTS</b>The malarial parasites and their fine structures were dramatically well preserved in the ghost erythrocytes, such as the ring-like early trophozoites, the brassiere-like early schizonts, the emerging buds on the surface of late schizonts, and the grape-cluster like late schizonts. The cytostome, food vacuole, and crystallized malarial pigments were found in the early trophozoites. The proliferations of nucleoplasma and nuclear membrane as well as and the clot-like nuclear division were followed by the budding during the schizogony.</p><p><b>CONCLUSION</b>The saponify technique that makes the erythrocytic malaria parasites and their fine organellae to be dramatically revealed in their ghost erythrocytes, may be a useful method in the Plasmodium biological research and anti-malaria immunological researches.</p>

Immunity of peritoneal monocytes against Plasmodium yoelii infected erythrocytes / 中国医学科学院学报

<p><b>OBJECTIVE</b>To test the immunity of peritoneal monocytes against Plasmodium yoelii infected red blood cells (target cells).</p><p><b>METHODS</b>Saponinized Plasmodium yoelii infected red blood cells (SPRBC, Ghost erythrocyte) were used to immunize mice i.p twice. Three weeks later, the infected red blood cells were injected i.p.; 90 min later, the total peritoneal cells were isolated and washed for scanning electromicroscopy to observe the effects of the peritoneal monocyte to the target cell.</p><p><b>RESULTS</b>The peritoneal cells of the immunized mice were activated after 90 min of the challenge of target cells. The size of the cell was not even and the pili on the cell surface turned to be long and densed. Cell interconnections were found among the cells. In some peritoneal monocytes, their cell plasma were scattered (omlette-like) or with the shape as "cellular bomb". The scattered or the sheeted pili and spredding cell plasma could adhere to the target cells which were perforated densely and damaged.</p><p><b>CONCLUSION</b>The protective adaptive immunity exists in the peritoneal monocytes of immunized mice.</p>

Molecular cloning of Plasmodium yoelii dynamin-like protein (PyDyn) gene and the immunological character of its domains / 中国医学科学院学报

<p><b>OBJECTIVE</b>To identify and clone a new full ORF gene of PyDyn (Plasmodium yoelii dynamin-like protein), and examine the protection of their expression products.</p><p><b>METHOD</b>Using the P. yoelii Genome technology and RT-PCR.</p><p><b>RESULTS</b>The full ORF gene of PyDyn was amplified from mRNA of the erythrocytic stage of P. yoelii., three domains of PyDyn were expressed in E. coli., and the fairly positive immunogenicity of them was showed by IFA. The full ORF gene of PyDyn was 2,433 bp and encode 811 amino acids. Its Gene Bank access number is AF458071. PyDyn belongs to the dynamin-like protein family according to its property.</p><p><b>CONCLUSION</b>The new full ORF gene of PyDyn is obtained and identified; their expressed domains are probably new candidates for malaria vaccine.</p>