In vitro regeneration of Hypericum patulum Thunb.--a medicinal plant.

A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot formation. Upon transfer to phytohormone free liquid medium following a brief exposure to auxin, root formation occurred from the micro shoots . Rooted plants were hardened and transferred to soil. Regeneration potentiality was found to be constant throughout the year in long term cultures.

Análise de vitaminas do complexo B em carnes preparadas em serviço de alimentaçäo / Complex B vitamins analyse in meats prepared in alimentation service

Apesar da evoluçäo tecnológica, estudos sobre a estabilidade de nutrientes em alimentos preparados em Serviços de Alimentaçäo têm recebido pouca atençäo. No entanto, é cada vez maior o número de pessoas que fazem suas refeiçöes nestes estabelecimentos. Uma vez que o conteúdo em vitaminas é um dos fatores mais importantes na determinaçäo da qualidade dos alimentos processados, o objetivo deste estudo foi definir métodos de análise quantitativa para tiamina, riboflavina e ácido nicotínico por CLAE em carnes, para, entäo, avaliar a influência da preparaçäo em Serviço de Alimentaçäo sobre o conteúdo destas vitaminas em diferentes cortes de carne bovina, suína e de frango. Objetivou-se também avaliar a estabilidade das vitaminas citadas após métodos de preparaçäo tradicionais comparados com métodos de preparaçäo em forno combinado (um dos equipamentos de cocçäo mais modernos utilizados em Serviço de Alimentaçäo)...

Algunos aspectos de intéres sobre la tiamina / Important aspects on thiamine

Se describen los factores que influyen en la absorción y el metabolismo de la tiamina, los antagonistas de esta vitamina y se discuten algunos resultados científicos sobre su deficiencia y la evaluación bioquímica del estado nutricional de este nutriente

Dosagem microbiológica de Tiamina (Vit.B1) em cultivares de soja (Glycine max) L. Merril

Utilizando-se métodos microbiológico, foi quantificada a vitamina tiamina (vit. B1) em cultivares de soja (Glycine Max) safras 1982(47) e 1984(96). Os valores encontrados demonstram que esta vitamina näo apresenta variaçöes quantitativas significativas entre as variedades genéticas analisadas, porém foi observada perda da atividade biológica desta vitamina durante a estocagem dos gräos

Effect of acephate (orthene) on tissue levels of thiamine, pyruvic acid, lactic acid, glycogen and blood sugar.

Effect of Acephate, an organophosphorus insecticide, on tissue levels of thiamine, pyruvic acid, lactic acid, glycogen and blood sugar, has been studied. The albino rats, injected subcutaneously with Acephate (25 mg/10 gm body wt./day) for 4 weeks and 8 weeks, showed appreciable depletion of thiamine in liver, heart, kidney, brain and blood. The depletion of thiamine was found to be more after 8 weeks of Acephate injection. There was concomitant increase in pyruvic acid and lactic acid in various tissues. There was enormous depletion of glycogen in liver and slight rise in blood sugar concentration. The animals injected thiamine (120 micrograms/100 gm body wt./day) along with Acephate, showed more or less normal levels of thiamine, pyruvic acid, lactic acid, liver glycogen and blood sugar. The increase in pyruvic acid and lactic acid in tissues has been attributed to depletion of thiamine which is required of pyruvic acid oxidation. The increase in blood sugar has been attributed to the excess breakdown of glycogen.

Riboflavin and Thiamine Absorption

For the purpose of estimating the maximum absorption of riboflavin and thiamine from the intestine, large test doses of riboflavin and thiamine were administered orally to apparently normal male subjects, receiving normal Korean diets, and their maximum absorption were estimated by determining the excretion in urine. The maximum absorption of riboflavin and thiamine were estimated 10.700 and 3.800 mg. respectively.