Differential Expression of Integrin β1 in Two Brain Injury Models of Rats / 法医学杂志

Objective To study the characteristics of positive expression of integrin β1 in the rat brain tissue of two kinds of traumatic brain injury models and to explore the feasibility of inferring the mode of traumatic brain injury using the positive expression of integrin β1. Methods The occipital region of rats was hit by hydraulic impact method and pendulum striking method to produce two closed brain injury models of linear and rotation acceleration respectively, then 120 SD rats were randomly divided into linear acceleration injury group, rotation acceleration injury group, sham operation group and normal control group. Immunohistochemistry staining and Western blotting method were used to detect the positive expression of integrin β1 in different parts of the brain tissue at 30 min, 3 h, 6 h, 12 h, 3 d and 7 d after rat injury. The data was processed statistically by SPSS 18.0 software. Results The positive expression of integrin β1 was detected 30 min after brain injury and reached the peak 6 h after brain injury. With the extension of injury time, the expression tended to enhance. At the same time points after injury, the differences in the positive expression of integrin β1 between the linear acceleration injury group and the rotation acceleration injury group in the occipital strike point and thalamus had no statistical significance （ P>0.05）, but the differences in the expression of integrin β1 in the frontal lobe and brain stem had statistical significance （P<0.05）. Conclusion The characteristics of positive expression of integrin β1 in brain tissue can be used to infer the strike point and the manner of injury and has application value for the reconstruction of craniocerebral injury process.

Expression of Fas protein in myocardiac tissue of viral myocarditis and dilated cardiomyopathy / 法医学杂志

OBJECTIVE@#To study the pathogenesis of viral myocarditis (VMC) and dilated cardiomyopathy (DCM) and their relationship.@*METHODS@#Sixty samples including 20 VMC, 20 DCM and 20 controls were collected. The expression of Fas protein in myocardium of each group was detected by modified immunohistochemistry with unequivocal brown staining in the myocardial membrane scored as positive, and the results of positive reaction were analyzed by Ridit test.@*RESULTS@#Fas protein expression increased obviously in VMC and DCM groups as compared with that of the control group. The difference of positive results between each group analyzed by Ridit test was statistically significant (P<0.005). Statistically significant differences were found between VMC and control groups as well as between DCM and control groups (P<0.05), but not between VMC and DCM groups (P>0.05) by multiple comparison Ridit test.@*CONCLUSION@#The expression of Fas protein is significantly higher in the VMC and DCM groups than in that of the control group. These results suggest that both the VMC and DCM may share a similar pathogenesis, which most likely involves cell apoptosis.

Bcl-2 expression following the brain concussion in rats / 法医学杂志

OBJECTIVE@#To evaluate the expression of Bcl-2 protein after brain concussion.@*METHODS@#Expression levels of Bel-2 protein in cortex, pontine and cerebellum of rats were investigated using immunohistochemistry.@*RESULTS@#There was no expression of Bcl-2 protein in control group seen. The expression of Bcl-2 protein in brain concussion groups was detected at l hour, and the expression level reached its peak 4 days after the concussion and then declined gradually.@*CONCLUSION@#Our findings suggest that the detection of Bel-2 protein could be an indicator for diagnosis of brain concussion and for estimation of the post injury time interval.

Apoptosis in myocardial cells after macleaya cordata total alkaloids poisoning in rats / 法医学杂志

OBJECTIVE@#To observe pathological changes and apoptosis in rats myocardial cells after Macleaya cordata total alkaloids poisoning, and to provide some references for Macleaya cordata total alkaloids poisoning detection.@*METHODS@#An experimental model of Macleaya cordata total alkaloids poisoning was established, and the technology of TUNEL staining was used.The results were analyzed by computer image analysis competitive system.@*RESULTS@#Quantities of apoptosis in myocardial cells in poisoning groups were much more than those in the control groups at different tages (P<0.01). In addition the quantities of apoptosis were different after different poisoning duration.@*CONCLUSION@#Although clinical symptoms was not obvious and could not be detected by poison analysis. Pathological changes induced by Macleaya cordata total alkaloids could be found through the apoptosis detection.