RESUMO
The effect of reserpine was studied alone and in combination with anticancer drugs on human chronic myeloid leukaemia (CML) cells. To study the effect of reserpine on anthracycline antibiotic adriamycin and anthracenedione mitoxantrone the extent of 3H-thymidine incorporation into the DNA was taken as the measure of cytotoxicity. The results indicate that reserpine enhances the cytotoxicity of mitoxantrone and adriamycin in mildly toxic concentrations (1 and 10 micrograms respectively), in CML cells. The mechanism of enhancement in drug sensitivity by reserpine in CML cells is due to enhanced intracellular accumulation of drug.
Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/uso terapêutico , Ensaios de Seleção de Medicamentos Antitumorais , Sinergismo Farmacológico , Humanos , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Mitoxantrona/uso terapêutico , Reserpina/farmacologia , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
Toxicological study was carried out in rats with chloroform-soluble fraction of the nuts of Semecarpus anacardium to determine its safe non-toxic dose. The fraction produced toxicity at all levels tested (50-400 mg/kg) but the extent of toxicity was found dose-dependent. At lower doses this fraction induced partial growth inhibition over 36 days and higher doses proved fatal within 6 days. It was observed that 230 mg/kg caused 50% mortality in rats and this value is 1380 mg/m2 when expressed for body surface area. This work will be of some use in the cancer chemotherapy study of the fraction.