Helicoacter pylori prevalence and resistance patterns in dyspeptic patients from Ismailia, Egypt

This study was aimed To determine the incidence of H. pylon with gastrointestinal disorders, to correlate its presence with endoscopic findings, and to determine the antibiotic resistance rates and patterns on isolates from dyspeptic patients in Ismailia, Egypt. Biopsy specimens were collected from 150 dyspeptic patients. One hundred and seven H. pylon isolates were isolated on selective Colombia blood agar and non-selective Brain heart infusion agar. Susceptibilities were determined for these isolates by disc diffusion methods.: The prevalence of positive H. pylon isolates among the dyspeptic patients was 71.33%. A strong association was found between H. pylon and duodenal ulcer more than gastric ulcer and gastritis. The resistance rate to metronidazole, tetracycline, clarithromycin, and amoxicillin was 86%, 7.4%, 5.6%, and 0.9%, respectively. Dual resistance rate for metronidazole-tetracycline was 4.6% and for metronidazole-clarithromycin was 3.7%. The rate of triple resistance was 1.8% for metronidazoletetracycline-clarithromycin and 0.9% for metronidazole- tetracycline- amoxicillin It may concluded that: Metronidazole resistance was a character of H. pylon isolates within most patients in Ismailia, while amoxicillin resistance is rare. Also combined resistance problem is growing due to the indiscriminate use of antibiotics. Antibiotics resistance monitoring is recommended continuously, since the successful treatment of H, pylon infection is becoming compromised by the emergence of resistance to antimicrobial. the disease

Role of plasma homocysteine concentration in type-1-diabetes with and without nephropathy

To determine the plasma level of total homocysteine [tHcy] as a marker of endothelial cell dysfunction and to investigate its possible role in type I diabetes [IDDM] with nephropathy. A randomized group comparative and cross sectional study. A total of 60 type I diabetic patients, 40 of them were complicated with nephropathy.20 patients without complication [s] and 20 healthy control subjects matched for sex and age. Suez Canal University Hospital. Fasting plasma concentration of tHcy was estimated by immunoenzymatic assay, hemoglobin was measured by affinity chromatographic method. Albumin excretion rate was determined by immunturbidometric method. Fasting blood glucose was determined by glucose oxidase method and serum creatinine was measured by alkaline picrate method. Type 1[IDDM] patients with nephropathy showed a significant increase of the fasting plasma tHcy levels compared to controls and those patients without complications. A significant positive correlation was observed between fasting tHcy and albumin excretion rate [AER] in patients with diabetic nephropathy. Also tHcy had positive significant correlation with serum creatinine and HbAlc. Meanwhile there was no significant correlation between tHcy and the following parameters, age, duration of diabetes and body mass index [BMI]. tHcy was increased in type I diabetic patients with nephropathy, tHcy was significantly correlated to AER, serum creatinine and HbAlc in type I diabetic patients with nephropathy tHcy contribute to enhanced rate of vascular complications observed in type I diabetic patients, tHcy can be an important risk factor that worsen prognosis

Role of plasma vascular endothelial growth factor [VEGF] in type-2 diabetes mellitus, with and without nephropathy, and its relation to glycemic control

To determine the plasma concentration of vascular endothelial growth factor [VEGF] and to investigate its possible role in diabetic nephropathy. A randomized, group comparative, and crossectional study. A total of 50 type-2 diabetic patients, 30 of them were complicated by nepthropathy, and other 20 diabetic patients without nephropathy, and 20-matched of non-diabetic control subjects. Suiz Canal University Hospital. Plasma concentration ol' VEGF, HbAlc, plasma glucose, albumin excretion rate [AER], and serum creatinine. The type-2 diabetic patients with and without nephropathy had plasma level of VEGF significantly higher than that of controls. Diabetic patients with nephropathy had significantly higher plasma level of VEGF than that of diabetics without nephropathy. Significant positive correlations exist between the plasma concentration of VEGF and fasting, 2-h postprandial blood glucose, glycosylated hemoglobin [HBAIc], and AER. VEGF may play an important role in mediating diabetic nephropathy