A study on genes related to cognitive dysfunction in elderly patients with atrial fibrillation / 中华老年医学杂志

Objective:To investigate the molecular genetic mechanisms of cognitive dysfunction in elderly patients with atrial fibrillation.Methods:A case-control study was conducted to analyze statistical data from a previous epidemiological survey, which used a stratified, random cluster sampling method and covered towns and communities in eastern, southern and northern Xinjiang.A total of 8529 residents aged ≥60 years were investigated, of whom, 301 patients with a history of atrial fibrillation or a definitive diagnosis of atrial fibrillation by electrocardiogram during investigation were selected.The patients with atrial fibrillation were divided into a cognitive impairment group(55 cases)and a normal cognitive group(246 cases)according to the diagnostic criteria of cognitive dysfunction.Genotypes of a GWAS on the main atrial fibrillation-related genes were analyzed for genes associated with atrial fibrillation, aging and cognitive impairment.Results:Unconditional multivariate Logistic regression analysis showed that mental work and non-solitary living were protective factors against cognitive impairment in the elderly with atrial fibrillation( OR=0.206, 95% CI: 0.048-0.873; OR=0.286, 95% CI: 0.089-0.922; all P<0.05). Klotho(rs571118)TT and the ApoE ε3/ε4 genotypes were independent risk factors for cognitive impairment in elderly patients with atrial fibrillation( OR=3.922, 95% CI: 1.326-11.595; OR=6.843, 95% CI: 1.263-37.078; all P< 0.05). The interaction between Klotho(rs571118)and ApoE ε3/ε4 was not associated with cognitive impairment in the elderly with atrial fibrillation( OR=1.552, 95% CI: 0.703-3.428; OR=1.897, 95% CI: 0.967-3.723; OR=0.496, 95% CI: 0.061-4.026; all P>0.05). Conclusions:Klotho(rs571118)TT and the ApoE ε3/ε4 genotypes may promote cognitive dysfunction in elderly patients with atrial fibrillation.The results may serve as a basis for research on the mechanisms of cognitive dysfunction in elderly patients with atrial fibrillation.

Association of KCNE1 and KCNE4 gene polymorphisms with atrial fibrillation among Uygur and Han Chinese populations in Xinjiang / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To assess the association of KCNE1 (rs1805127) and KCNE4 (rs12621643) polymorphisms with atrial fibrillation (AF) among ethnic Uygur and Han Chinese in Xinjiang.</p><p><b>METHODS</b>A case-control study was carried out. The patients and controls were selected based on ethnicity, gender and age with an 1:1 ratio. DNA was extracted from peripheral blood samples. Genotypes of KCNE1 (rs1805127) and KCNE4 (rs12621643) were determined with a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.</p><p><b>RESULTS</b>Multivariate Logistic regression analysis showed KCNE1 (rs1805127) to be an independent risk factor for AF among Uygurs, while KCNE4 (rs12621643) was a risk factor for both Uygur and Han patients with AF (P < 0.05). The population attributable risk percentage (PARc%) of obstructive sleep apnea hpoventilation syndrome, obesity, hypertension, cholesterol, Hcy, hs-CRP, IL-6, KCNE1 (rs1805127) and KCNE4 (rs12621643) were 9.68%, 12.06%, 15.76%, 6.91%, 11.37%, 17.78%, 9.31%, 11.27% and 6.46% among the Uygurs, respectively. The PARc% of drinking, hypertension, cholesterol, Hcy, hs-CRP, IL-6, and KCNE4 (rs12621643) were 12.94%, 14.48%, 7.24%, 8.49%, 17.29%, 9.49% and 7.41% among Hans.</p><p><b>CONCLUSION</b>The KCNE1 (rs1805127) appears to an independent risk factor for AF in the Uygur population. And the KCNE4 (rs12621643) was an independent risk factor for AF among both Uygurs and Hans. Management of the risk factors of AF based on testing of "risk genes" may have an impact on the prevention and treatment of AF.</p>

Epidemiological survey of atrial fibrillation among Uygur and Han elderly people in Xinjiang Uygur autonomous region / 中华流行病学杂志

Objective To investigate prevalence of atrial fibrillation (AF) in Uygur and Han elderly populations in Xinjiang Uygur autonomous region (Xinjiang).Methods Epidemiological survey was conducted among the residents selected through stratified random cluster sampling in the southern,northern and eastern Xinjiang.Results The overall AF prevalence among Uygur and Han elderly people was 3.56％.The crude prevalence of AF was 2.91％ among Uygur elderly people and 4.13％ among Han elderly people.The sex specific prevalence of AF were 3.19％ and 2.61％ among Uygur males and females respectively,and 5.01％ and 3.31％ among Han males and females respectively.The prevalence of valvular AF among Uygur ethnic group was higher than that in Han ethnic group;the prevalence of non-valvular and isolated AF in Han ethnic group were higher than those in Uygur ethnic group.The compliance of aspirin and β-blocker medication among Han ethnic group was better than that in Uygur ethnic group.The compliance of warfarin medication was poor in both Uygur ethnic group and Han ethnic group.The prevalence of ischemic stroke were 8.82％ and 0.98％ in Uygur elderly people with or without AF.The prevalence of ischemic stroke were 6.08％ and 0.70％ in Han elderly people with or without AF.Conclusion The prevalence of AF in elderly people in Xinjiang is similar to the results from other domestic studies,the prevalence of AF in Han elderly people was higher than that in Uygur elderly peoples.

Association of vitamin D receptor gene polymorphisms with mild cognitive impairment among elderly ethnic Uygurs / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To assess the association of vitamin D receptor gene (VDR) Apa I, Bsm I genotypes and allele frequencies and mild cognitive impairment (MCI) among elderly ethnic Uygurs from Xinjiang, China.</p><p><b>METHODS</b>The polymorphisms of the VDR genotypes (Apa I and Bsm I) were analyzed by the SNaPshot method in 124 MCI patients and 124 controls.</p><p><b>RESULTS</b>Factors which can increase the risk for MCI have included the A allele of the Apa I polymorphism [OR=1.62, 95%CI(1.13-2.31)] and the AA genotype [OR=3.49, 95% CI(1.57-7.74)], the T allele of the Bsm I polymorphism [OR=1.94, 95%CI(1.24-3.05)], higher triglyceride and systolic blood pressure levels.</p><p><b>CONCLUSION</b>Polymorphisms of the VDR gene including the A allele and AA genotype of Apa I, and the T allele of Bsm I are probably associated with MCI among elderly ethnic Uygurs, and so are higher levels of triglyceride and systolic blood pressure.</p>

Related factors for Alzheimer's disease in Han versus Uygur populations in Xinjiang Uygur Autonomous Region / 中华老年医学杂志

Objective To investigate the related factors for Alzheimer's disease(AD) in Han versus Uygur populations in Uygur Autonomous Region.Methods A case-control study was conducted.Firstly,people over 50 years old were involved in epidemiology inquisition in Xinjiang Uygur Autonomous Region.Then we screened for dementia by MMSE and scrutinized the person who got the score of MMSE for illiteracy(≤17),elementary school(＜20),middle school and above it(＜ 24)by Hachinski Ischemic Score(HIS) and activity of daily living scale(ADL).Finally,AD cases were diagnosed by standard of the national institute of neurologic,communicative disorders and stroke and AD-related disorders association (NINCDS-ADRDA),and controls were selected based on age roughly.Univariate and multivariate analysis of related factors for AD in the two populations were conducted by using logistic regression mode.Results There were 109 AD cases and 218 controls in Han population and 127 AD cases and 254 controls in Uygur population.Logistic regression analysis showed that the risk for AD in Han population was increased by 3.078,1.369 and 2.794 folds respectively in women,people with high level of serum total cholesterol and people with low-density lipoprotein cholesterol as compared with men,people with normal level of serum total cholesterol and people with low-density lipoprotein cholesterol(OR =3.078,1.369 and 2.794,respectively,all P＜ 0.05); and the risk for AD in Uygur population was increased by 3.279,3.447,1.407 and 1.746 folds respectively in women,people with brain trauma history,and people with high level of serum triglyceride and total cholesterol as compared with men,people without brain trauma history and with normal level of serum triglyceride and total cholesterol(OR=3.279,3.447,1.407 and 1.746,all P＜ 0.05).Conclusions The study demonstrates that female and high total cholesterol are the risk factors for AD in both of Han and Uygur populations.High level of low-density lipoprotein cholesterol is one of the risk factors for AD in Han population,and brain trauma history and high level of serum triglyceride are risk factors for AD in Uygur population.

Association between mild cognitive impairment and hypersensitive C reactive protein, interleukin-1β and interleukin-6 in Xinjiang region, China / 中华神经科杂志

Objective To explore the association between mild cognitive impairment (MCI) and hypersensitive C reactive protein (hs-CRP),interleukin-1β (IL-1β) and interleukin-6 (IL-6) among Uygurs and Hans in Xinjiang region,China.Methods From July 2008 to October 2010,the epidemiological investigation was performed in Southern,Eastern and Northern of Xinjiang.Based on the diagnostic standard of United States psychiatric society of spirit obstacles diagnosis and statistics manual Ⅳ amendment version in the mild cognitive function,483 MCI patients were diagnosed.Finally,314 MCI patients were selected from above according to the completion of data.Moreover,299 subjects were randomly selected as the control group from the investigation.General information and fasting plasma were collected,and blood glucose,blood lipid and biochemical indexes,serum hs-CRP,IL-1β,IL-6 of concentration were tested.The association between MCI and hs-CRP,IL-1β and IL-6 were analyzed with SPSS 17.0 software.Results (1) The concentrations of serum hs-CRP,IL-1β and IL-6 in MCI group (3.40 (6.53) mg/L,0.09 (0.09) ng/L,136.08(96.77) pg/L) were significantly higher than that in control group (2.99 (3.91) mg/L,0.07(0.06) ng/L,79.32(68.79) pg/L) respectively (Z =-2.525,-2.946,-9.361,all P ＜0.05).(2)The concentrations of serum hs-CRP,IL-1β,IL-6 in Han MCI patients were significantly higher than that in Han non-MCI subject; The concentrations of serum IL-1β,IL-6 in Uygur MCI patients were significantly higher than that in Uygur non-MCI subjects; However,the hs-CRP concentration between MCI and non-MCI group is not statistically different among Uygurs.(3)Non-conditional Logistic regression analysis showed that serum IL-1β(OR =1.008,95％ CI0.897-1.071,P =0.006),hs-CRP (OR =1.096,95％ CI1.056-1.137,P =0.000),IL-6 (OR =1.011,95％ CI1.008-1.014,P =0.000) were associated with MCI.Conclusion The hs-CRP,IL-1β and IL-6 were independent risk factors for MCI.

Epidemiological study of obesity in middle-aged and elderly people of Uygur and Han populations in xinjiang / 中华内分泌代谢杂志

According to the recommended diagnostic criteria of obese adults in Asia-Pacific region,8 284 cases of middle-aged and elderly people in Uygur and Han populations were collected randomly to study the status and distribution characteristics of two nationalities.Standardized prevalences of obesity were 20.1 2%in Uygurs and 23.077%in Hans.Woman in Hans reached the highest prevalence.Alcohol-drinking,abnormal giycometabolism,and serum cholesterol levels were the most relevant risk factors.

Determination of Diclofenac Sodium in Huamn Plasma by HPLC / 中国药房

OBJECTIVE:To establish a HPLC procedure to determine diclofenac sodium in human plasma.METHODS:The plasma sample was deproteinized by adding acetonitrile,A Hypersil-BDS(250?4.6mm,5?m)column with a mobile phase consisting of methanol-water-10%phosphate acid(60∶40∶1)was used,the detecting wavelength was276nm,column tem?perature was35℃.RESULTS:The linear range was20.50～2050.0ng/ml(r=0.9998,n=5),the detective limit was10ng/ml.The recovery was99.47%～105.4%with the within-day RSD from3.77%to5.81%(n=5),and the between-day RSD from4.93to8.96%(n=5)at three different concentrations.CONCLUSION:The method is simple,rapid and suitable for the pharmacokinetic study of diclofenac sodium.

Determination of magnolol and honokiol in Huoxiangzhengqi Capsule by SFE-CGC / 中成药

Objective: To determine magnolol and honokiol in Huoxiangzhengqi Capsule(oil of Herba Agastachis, oil of Perillae, Pericarpium Citri Reticulatae, Cortex Magnoliae Officinalis, etc.).Methods: A supercritical Fluid Extraction (SFE) has been used to extract magnolol and honokiol in Huoxiangzhengqi Capsule, and CGC was used to analyse the extracts.Results: The average rccovery was 96.81% ( RSD =2.81%, n =5) for magnolol, and 97.75% ( RSD =2.36%, n =5) for honokiol, respectively. Conclusion: The SFE CGC offline method for the analysis of Huoxiangzhengqi capsule is simple, quick and accurate.

Bioequiavailability of Hydrochloric Itraconazole Capsule in Human Body / 中国药房

OBJECTIVE:To evaluate the human body pharmacokinetics and bioequiavailability of two kinds of oral single dose of hydrochloric itraconazole capsules.METHODS:A randomized,crossover study of20healthy volunteers receiving sin-gle oral dose of200mg itraconazole was conducted with in vivo blood concentrations determined by HPLC-fluorescence de-tection.RESULTS:The pharmacokinetic parameters for the testing itraconazole and the reference itraconazole were as fol-lows,t 1/2 were(29.3?5.62)h and(29.3?5.81)h,respectively;C max were(81.4?60.0)?g/L and(77.8?45.2)?g/L,respec-tively;t max were(3.9?0.70)h and(4.2?0.70)h,respectively;AUC 0～72 were(1199.4?649.6)(?g?h)/L and(1174.3?701.9)(?g?h)/L,respectively;AUC 0～∞ were(1414.0?815.2)(?g?h)/L and(1386.1?735.8)(?g?h)/L,respectively.there were no significant differences in main pharmacokinetics parameters between2preparations,except in t max from analysis of variance and one-side&two-sides t-tests.The relative bioavailability of trial itraconazole capsule was(105.3?23.4)%.CONCLUSION:These two kinds of itraconazole capsules are bioequivalent.

Influence of Ciprofloxacin on the Pharmacokinetics of Tacrolimus in Rabbits / 中国药房

0.05).CONCLUS_ION:The present study in rabbits showed no significant interaction between ciprofloxacin and tacrolimus after combined administration of these two drugs in a single dose.

Determination of plasma loratadine concentration with RP-HPLC and its pharmacokinetic study / 第二军医大学学报

Objective : To establish a RP-HPLC method for the determination and pharmacokinetic study of loratadine in Chinese healthy volunteers. Methods; Chrornatography was performed on a Hypersil-BDS with acetonitrile-water (65 : 35, pH was adjusted to 3. 8 with H3PO4) as mobile phase. The flow rate was 1. 0 ml/min and the UV wavelength was set at 248 nm. Results: Good linearity was found within 0. 506 0-50. 60 ng/ml of loratadine in human plasma(r = 0. 999 5). The mean relative recovery rate was more than 95% ; intra-day and inter-day RSD were less than 15. 0%. The limit of detection was 0. 506 ng/ml. The main pharmacokinetic parameters were as follows:t12 was (1. 57 ? 0. 18) h;MRT was (2. 46?0. 19) h;Cl/ F was (583. 7?215. 0) L/h;cmax, was (37. 40?8. 92) ng/ml;tmax was (1. 0?0. 20) h;AUC0-8h was (73. 24?21. 30) ng ? h/ ml;.AUC/C0-∞ was (75. 57?21. 70) ng ? h/ml. Conclusion: Our method has a good selectivity and sensitivity and can be used for further clinical pharmacokinetic study of loratadine.