dalbinol induces apoptosis of human colon cancer cells through ROS/Dvl/GSK-3β/β-catenin pathway / 中国药理学通报

Aim To investigate the effects of dalbinol on proliferation and apoptosis of human colon cancer HCT1 16 cells and its mechanisms.Methods Anti-proliferative effect of dalbinol was evaluated by MTT assay.The morphological changes of apoptosis were observed by Hoechst33342 staining.Apoptotic rate and ROS generation were analyzed by flow cytometry.The related proteins of Wnt/β-catenin pathway and the ap-optosis-associated proteins expression were measured by Western blot.Results The growth of HCT1 16 treated with dalbinol was inhibited in a dose and time dependent manner with IC50 (4.8 ±0.53 ),(2.5 ± 0.43)and (0.6 ±0.22)μmol·L-1 at 24,48 and 72 h,respectively.Typical morphological changes of ap-optosis such as cell shrinkage,karyopyknosis and nu-clear condensation were observed by Hoechst33342 staining.Meanwhile,the apoptotic rate and intracellu-lar ROS generation of dalbinol were both increased dose-dependently. Western blot results showed that dalbinol could activate the expression of cleaved Caspase-3 and cleaved PARP by decreasing anti-apop-totic protein levels such as Bcl-2 and Mcl-1 and in-creasing pro-apoptotic protein levels such as Bax and Bim,which induced further apoptosis.Moreover,dal-binol can reduce the protein expression of the total and nuclear β-catenin,but not cytoplasmic β-catenin by suppressing the protein expression of Dvl-2 and GSK-3β(pS9 ),as well as its target proteins c-Myc and Sur-vivin.Conclusion dalbinol can induce apoptosis in colon cancer HCT1 16 cells by upregulating the intra-cellular ROS generation and suppressing Dvl/GSK-3β/β-catenin pathway.

Discussing on bringing conductive education into the compulsory course of rehabilitation ther-apy related majors / 中华医学教育探索杂志

Conductive education is an education system aiming at helping the handicapped people improve the impaired and abnormal function through education. This article started from the current status of conductive education in clinical rehabilitation and analyzed the causes for imbalance between supply and demand of conductive education professionals. Meanwhile strengthening and ex-panding the coverage and strength of general conductive education was proposed and bringing con-ductive education into the compulsory course of rehabilitation therapy major was recommended. Finally, training conductive educational professionals with high-quality was regarded as the most fundamental measure to solve these problems.

Creation and Application of Conductive Education-Rehabilitation Rhythmic Exercise for Cerebral Palsy / 中国康复理论与实践

@#Objective To introduce a set of rehabilitation approach called Conductive Education-Rehabilitation Rhythmic Exercise andits application. Methods Control group and rehabilitation group were set up with 75 cerebral palsy children in each. And it was used once aweek for 3 months. Results The scores and effective rate of movement, speech communication, perception and cognitive in the rehabilitationgroup were higher than the control group (P<0.01). Conclusion Conductive Education-Rehabilitation Rhythmic Exercise was effectiveto cerebral palsy.

Creation and Application of Conductive Education-Rehabilitation Rhythmic Exercise for Cerebral Palsy / 中国康复理论与实践

@#Objective To introduce a set of rehabilitation approach called Conductive Education-Rehabilitation Rhythmic Exercise andits application. Methods Control group and rehabilitation group were set up with 75 cerebral palsy children in each. And it was used once aweek for 3 months. Results The scores and effective rate of movement, speech communication, perception and cognitive in the rehabilitationgroup were higher than the control group (P<0.01). Conclusion Conductive Education-Rehabilitation Rhythmic Exercise was effectiveto cerebral palsy.

Study of immunogenicity of chlorogenic acid-BSA with different number of CGA / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the immunogenicity of chlorogenic acid-BSA(CGA-BSA) and the foundation for the allergization of CGA-BSA.</p><p><b>METHOD</b>The CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by ELISA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer.</p><p><b>RESULT</b>When the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest.</p><p><b>CONCLUSION</b>It has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.</p>

Study on sensitization and mechanism of CGA-BSA / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the sensitization and mechanism of artificial antigen of chlorogenic acid (CGA-BSA).</p><p><b>METHOD</b>Using intensive immunization to establish allergy animal model on guinea pig and preparing antiserum and tissue for further test. Using HE staining to observe pathology change of lungs, trachea, liver. Using passive mast cell (PMC) degranulation test to observe the immunogenicity of CGA-BSA and using ELISA to detect IgE and histamine in plasma.</p><p><b>RESULT</b>There established allergy animal model on guinea pig, which include a increase cell degranulation by a ratio (63.58 +/- 10.23)% in PMC test, increase of specific antibody IgE and increase of histamine in plasma after provocation by ELISA.</p><p><b>CONCLUSION</b>Allergen CGA-BSA could provoke allergenic response in guinea pig, and the allergic response belongs to type I allergy.</p>

Antidepressant-like effects of piperine and its neuroprotective mechanism / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the antidepressant effect of piperine and its neuroprotective mechanism.</p><p><b>METHOD</b>The behavioral studies were performed in forced swimming test (FST) and tail suspension test (TST). To further explore the mechanisms underlying their antidepressant-like activities, CORT-induced neuroblastoma SH-SY5Y cells and isolated and cultured neural progenitor cells. By using MTT assay, the effect of piperine on neural cells proliferation was observed.</p><p><b>RESULT</b>The research results indicated that after a week of administration, piperine (10, 20 mg x kg(-1)) could significantly reduce the duration of immobility in both FST and TST. Piperine has the protective effect on neuroblastoma cells and increased proliferation of hippocampus neural progenitor cells.</p><p><b>CONCLUSION</b>In the present study, we demonstrated that the antidepressant-like effects of piperine and its mechanisms might be involved by up-regulation of the progenitor cell proliferation of hippocampus and cytoprotective activity.</p>

Effects of cembrane-type diterpenes on proliferation of PC12 cells and their antagonistic effects on neurotoxicity induced by glutamate / 中西医结合学报

To investigate the effects of cembrane-type diterpenes extracted from Sinularia flexibilis on the proliferation of PC12 cells and their protective effects on PC12 cells exposed to glutamate.

Antidepressant effects of piperine and its neuroprotective mechanism in rats / 中西医结合学报

To study the antidepressant effects of piperine in chronic unpredictable mild stress (CUMS) rats and to explore the underlying mechanisms in the hypothalamic-pituitary-adrenal (HPA) axis.

Regional intra-arterial infusion chemotherapy for pancreatic cancer of dogs with the mixture of lipid emulsion-cisplatin: an experimental study / 中华放射学杂志

Objective To approach the mechanism and efficacy of regional intra-arterial infusion chemotherapy with the mixture of lipid emulsion-CDDP (LE-CDDP) for treatment of locally advanced pancreatic cancer. Methods Twenty-four health dogs were divided into four groups (group A, B, C, and D). The dosage of CDDP was used in 4 mg/kg/body weight for each animal The 20% LE, as a solvent, was used in the experimental animals with 2 ml/kg/ body weight (group A) and 1 ml/kg/bedy weight (group B), respectively. Normal sodium (NS) as a solvent was used as control with 2 ml/kg/bedy weight (group C) and 1 ml/kg/body weight (group D), respectively. The LE-CDDP mixture and the NS-CDDP mixture were infused into the proximal segment of splenic artery under the DSA, with transfemoral arterial approach. Blood samples were collected after infusion at 0,3,5,10,20,30,40,50,60 min and the tissues were obtained after the 60 min's blood sample was collected. Blood samples, absorbent gland in peripancreas, liver, spleen, kidney, heart, portal vein, the superior segment of jejunal and pancreas and parapancreatic tissues were obtained for CDDP concentration analysis and histopatholngic examination. Results The values of the area under curve (AUC), the incipient serum concentration ( C0 ) and the elimination half-life (t1/2 ) of the serum CDDP concentration-time curve in four groups were A (54. 5 ± 10.1)%,(2.6±0.5) mg/L, (16.7±3.6) min;B (18.3±6.0)%,(1.5±0.2) mg/L, (47.9 ± 11.1) min; C (116.7±20.6)%, (6.5±0.4) mg/L, (10.5±2.8) min and D (126.6±30.7)%, (5.5±0.4) mg/L, ( 10. 1±3. 1 ) min, respectively. There were significant difference among these four groups ( F(AUC) = 42. 42, F(C0) = 249. 61, F( t1/2 ) = 12. 48, P < 0. 01 ). The values of AUC and C0 in the group A were significantly lower than those in the group C (t(AUC) = 6. 64,t(C0) = 16. 34, P <0. 01 ), and the corresponding values in the group B being also significantly lower than those in the group D (t(AUC) = 8.49, t(C0) =22. 30, P<0. 01 ). The value t1/2 in the group A was significantly longer than that of in the group C ( t = 3.36, P < 0. 01 ), and that of group B was also significantly longer than that of group D ( t = 3.71, P <0. 01 ). The values of AUC and C0 in the group B were significantly lower than those in the group A (t(AUC) = 7. 57, t(C0) = 5.48, P < 0. 01 ), and the value t1/2 in the group B was significantly longer than that in the group A (t = 3.22, P < 0. 05 ). The concentrations of the left lobe and horn of pancreas were higher in the group B (0. 18, 0. 18 mg/L) than those in the group A (0. 05, 0. 05 mg/L) (t =2. 52, 2. 73, P < 0. 05). The tissue CDDP concentration of the right lobe of pancreas and spleen were no significant difference between group A ( 0. 11, 0. 29 mg/L ) and group B ( 0. 07, 0. 24 mg/L) ( P > 0. 05 ). Perivascular lymphocytic and neutrophilic infiltration, congestion and hemorrhage were found in the pancreas, parapancreatic absorbent gland, liver and spleen in the group A and group B. The micro-particles of intralipid were present in the capillary vessel of these tissues. No specific pathological changes were found in other groups and organs. Conclusions The regional intra-arterial infusion with LE-CDDP mixture could increase the pancreatic CDDP concentration, meanwhile, it also could decrease the serum CDDP concentration. The more of the CDDP concentration in the LE-CDDP mixture, the more CDDP concentration at the pancreatic tissue accordingly.

Investigation of A New Economic and Practical Modality:Six-in-One Rehabilitation Approach for All-round Rehabilitation of Children with Cerebral Palsy:50 Cases Report / 中国康复理论与实践

@#Objective To investigate an economic and practical modality for the all-round rehabilitation of children with cerebral palsy(CP).Methods The six-in-one rehabilitation approach was designed according to the principal of modern rehabilitation and conductive education.It was mainly conducted through physical therapy,occupational therapy,speech therapy,sensory integration,supplemented with individual education and a self-originated "movement rehabilitation exercise".50 CP children were treated with this approach.Results After one period of treatment by six-in-one approach,the movement function,language ability and cognitive ability of those CP children improved tremendously.The children also got much progress in sociality and activities of daily living(ADL).The effective rate of rehabilitation reached 90%～96%.Conclusion The CP children have benefited variously in areas of language,movement,cognition,behavior,sociality and ADL from six-in-one approach,which requires small financial contribution from family and is cost-effective to the rehabilitation center.It is a new approach for all-round CP children rehabilitation,which can be adopted by small and middle sized rehabilitating institution and community rehabilitation,especially for those poor families CP children more than 3 years old.