Expression and methylation status of IGFBP-rP1 gene in laryngocarcinoma / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the relationship between the promoter methylation and protein expression of insulin-like growth factor binding protein-related protein 1(IGFBP-rP1) in laryngeal squamous cell carcinoma (LSCC).@*METHOD@#Methylation specific PCR (MSP) approach and immunohistochemistry methods were used to examine the methylation status and protein expression of IGFBP-rP1 in 45 samples of laryngeal carcinoma and 18 samples of tissues beside cancer.@*RESULT@#For the promoter site, methylation frequency of IGFBP-rP1 in tumor specimens (33.3%, 15/45) was significantly higher than that in corresponding normal tissues (5.6%, 1/18) (P < 0.05). The protein expression of IGFBP-rP1 in tumor tissues was significantly higher than that in corresponding normal tissues (P < 0.05) and was inversely correlated with its methylation status of promoter.@*CONCLUSION@#Epigenetic silencing of IGFBP-rP1 gene expression by promoter hypermethylation may play an important role in LSCC.

Study on Bioequivalence of Finasteride Tablets in Human Body / 中国药房

OBJECTIVE:To study the human body bioequivalence of finasteride tablets from both domestic and im?port.METHODS:The serum concentration of18volunteers were determined by HPLC after a random cross single oral dose of10mg of finasteride tablets,the pharmacokinetic parameters and the relative bioavailability were calculated and the bioequiva?lence was evaluated as well.RESULTS:For the domestic and the import finasteride tablets,the AUC (0～t) of which were(990.80?302.80)(?g?h)/L and(1007.46?333.65)(?g?h)/L respectively,the C max of which were(119.22?18.48)?g/L and(114.08?22.97)?g/L respectively,the t max of which were(2.89?1.02)h and(2.64?0.54)h respectively,the relative bioavailability of the domestic preparation was(103.35%?29.75)%of the imported one.CONCLUSION:The domestic and import finasteride tablets were of bioequivalence.

Preparation and Quality Control of Qingyan Tea Bag / 中国药房

OBJECTIVE:To prepare Qingyang tea bag and establish the standard of quality control.METHODS:The Flos Lonicera and Radix Glycyrrhize were identified by TLC.The content of chlorogenic acid,the main active component in the tea derived from Flos Lonicera,was determined by TLCs.RESULTS:Linearity was found in the range from1.2?g～4.8?g of chlorogenic acid,r=0.9993;the average recovery was99.50%and RSD=2.39%.CONCLUSION:The method was found to be simple,precise and may be used for the quality control of the preparation.

CT Diagnosis in Tuberculosis of the Brain / 昆明医科大学学报

To evaluate CT in the diagnosis of tuber culosis of the brain． CT findings in 95 cases with tuberculosis of t he bran confirmed clinically were analyzed retrospectively． Results: The CT detectability fate was 80%． The direct CT si ngs included exudation in basal system, meningitis, enhancement and intracranial tuberculosi s． The indirect signs had hydrocephalus infarction, atrophy calcification, periventricular hypo densith and subdural fluid accumulation etc． The most usual appearance was hydrocephalus． Conclusions: (1) It was helpful to diagnose when very two kinds of direct sings or a kind of dir ect sings and more than two kinds of indirect sings appeared． (2)The plain and contrast canning s hould be routinely used in tuberculosis of the brain．

Brief Discussion Dispute in Pharmaceutical Care and Its Prevention / 中国药房

OBJECTIVE:To discuss the disputes in pharmaceutical care and the relevant preventive measures.METHODS:The disputes in pharmaceutical care and their reasons were analyzed,and the countermeasures were put forward.RESULTS&CONCLUSION:The countermeasures against disputes in pharmaceutical care are as follows.Firstly,the conception of“patient first and foremost”in pharmaceutical care should be established.The pharmaceutical service should be improved,and the mis?takes in dispensing and pricing of drugs should be prevented by establishing the particular system of two persons checking the prescription.Secondly,the drugs should be arranged in a scientific way,and the shortage of drugs should be prevented,while the control of drugs’expiration dates should be reinforced.Thirdly,the proper arrangement of human resources should be made.Last but not the least,the knowledge about rational drug use should be publicized,and the monitoring on ADR of drug should be developed.