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OBJECTIVE: To find disease biomarkers of cerebral ischemia by establishing cerebral ischemia-reperfusion injury model of rats and comparing with healthy rats. METHODS: Cerebral ischemia-reperfusion model of rats was established by modified Longa′s intraluminal suture method. After 24 h of modeling, plasma and brain tissue from the model group and the control group were collected for plasma biochemical indicators and metabolites analysis. Brain slices and plasma biochemical indicators were used to demonstrate the successful modeling. Gas chromatography-mass spectrometry was adopted to conduct targeted metabolomics studies on free fatty acids in rat plasma, tridecanoic acid (C13∶0) and 2,6-di-tert-butyl-4-methylphenol (BHT) were added as internal standard and antioxidant during sample preparation. Methylation was carried out using concentrated sulfuric acid/CH3OH as a derivatization reagent, and a non-polar stationary phase column was selected for chromatographic separation. RESULTS: Comparing the model group with the blank group, four differential metabolites were obtained, namely palmitic acid, stearic acid, oleic acid and linoleic acid, which were up-regulated throughout the metabolic pathway. The RESULTS of the relevant metabolic pathway analysis showed that the linoleic acid metabolic pathway had significant changes in the disease process. CONCLUSION: In the fatty acid anabolic pathway, the linoleic acid metabolic pathway changes significantly during the onset and treatment of cerebral ischemia-reperfusion. The effect of preventing and treating cerebral ischemia-reperfusion injury can be achieved by controlling the level of linoleic acid. This study evaluates the prognosis of cerebral ischemia reperfusion injury by measuring the plasma of model rats, explores its cause and pathogenesis, and laid a foundation for the study of disease pathology and the mechanism of drug action.
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In this paper, a case of pleural amoebic empyema and its diagnosis and treatment were reported.
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In this paper, a case of pleural amoebic empyema and its diagnosis and treatment were reported.
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Objective To investigate the effect of the correction of the position error of the abdominal tumor by cone beam scanning. Methods Six patients with abdominal tumor were selected with double-blind method, and electronical portal imaing device was used to collect the information of the patient dosimetry during radiotherapy.EDOSE tool was applied to 3D dose reconstruction so as to execute real-time 3D dose verification of the treatment plan. Each patient had 5 sets of data acquired when image guidance existed and another 5 sets when image guidance was absent. The treatment effects with or without image guidance were compared.Results The overall γ passing rate with cone beam CT image guidance gained no advantages over that without image guidance, though the setup error was corrected to some extent. Image guidance contributed nothing to increasing target area coverage while decreasing exposure dose to normal tissue. Conclusion Setup error correction by the image guidance based on bone marker has no significant effect on improving 3D dose verification and dose accuracy during the radiotherapy of the abdominal tumor patient.
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OBJECTIVES@#To observe the changes of cystathionine β-synthase （CBS） expression in the cerebral cortex after brain contusion at different times.@*METHODS@#An experimental model of traumatic brain injury （TBI） in mice was established by an improved weight-drop device. Then Western blotting and immunohistochemical examination were used to detect the CBS expression in cerebral cortex around injury at different time points （1 h, 6 h, 12 h, 1 d, 2 d, 3 d, 7 d）.@*RESULTS@#The results of Western blotting revealed that the expression level of CBS was down-regulated and reached its lowest level at the 3rd days after injury, and then restored to normal level after 7 days. The results of immunohistochemistry showed that CBS was present in the normal brain cortex. CBS expression gradually decreased at the 3rd days after injury, and then restored to normal level after 7 days.@*CONCLUSIONS@#CBS has the potential to be a reference index for time estimation after brain contusion in forensic practice.
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Animais , Masculino , Camundongos , Western Blotting , Encéfalo , Contusão Encefálica/patologia , Lesões Encefálicas/patologia , Córtex Cerebral/patologia , Cistationina beta-Sintase/metabolismo , Regulação para Baixo , Imuno-Histoquímica , Fatores de TempoRESUMO
OBJECTIVE@#To observe the time-course expression of calcium-calmodulin dependent protein kinase II delta (CaMK II delta) in cerebral cortex after traumatic brain injury (TBI).@*METHODS@#The TBI rat model was established. The expression of CaMK II delta in cerebral cortex around injured area was tested by Western blotting and immunohistochemical staining.@*RESULTS@#Western blotting revealed expression of CaMK II delta in normal rat brain cortex. It gradually increased after TBI, peaked after 3 days, and then returned to normal level. The result of immunohistochemical staining was consistent with that of Western blotting.@*CONCLUSION@#The expression of CaMK II delta around injured area after TBI increased initially and then decreased. It could be used as a new indicator for wound age determination following TBI.