RESUMO
To study the impact of the enterovirus 71(EV71) on the nuclear transport mechanism,The pGFP-NLS vector with nuclear location signal(NLS) was constructed, RD cells transfected by the pGFP-NLS vector were inoculated with the EV71 or cotransfected by EV71-2A vector. The results showed that GFP protein with NLS was expressed in the cytoplasm due to the inhibition of nuclear transport. In order to further study the mechanism of the EV71 to prevent nuclear transport,Nup62 was detected by Western blotting after RD cells were infected with EV71 or transfected by EV71-2A vector. The results showed that decreased expression of Nup62 could be detected after infection with EV71 and transfection by EV71-2A vector. This study demonstrates that the cleavage of Nup62 by EV71 2A protease may be the mechanism of nuclear transport inhibition.
Assuntos
Humanos , Transporte Ativo do Núcleo Celular , Linhagem Celular Tumoral , Núcleo Celular , Metabolismo , Enterovirus Humano A , Genética , Metabolismo , Infecções por Enterovirus , Virologia , Regulação Viral da Expressão Gênica , Vetores Genéticos , Proteínas de Fluorescência Verde , Metabolismo , Glicoproteínas de Membrana , Metabolismo , Sinais de Localização Nuclear , Metabolismo , Complexo de Proteínas Formadoras de Poros Nucleares , Metabolismo , Peptídeo Hidrolases , Metabolismo , Proteínas Recombinantes de Fusão , Metabolismo , TransfecçãoRESUMO
<p><b>OBJECTIVE</b>To study the expression of gastrin(GAS) and gastrin releasing peptide(GRP) in patients with gastric cancer and investigate the clinical significance.</p><p><b>METHODS</b>The expression of GAS and GRP in sixty patients with gastric cancer was detected by using tissue chip technique and immunohistochemical methods.</p><p><b>RESULTS</b>The positive rates of GAS and GRP were 30.0% and 11.7% respectively in 60 cases with gastric cancer. The positive rates of GAS and GRP were higher in moderately and poorly differentiated cancers than those in well differentiated cancer (P< 0.05). The positive rates of GAS and GRP were significantly higher in mucinous adenocarcinoma and signet-ring cell carcinoma than those in other types of gastric cancer (P< 0.05). The positive expression of GAS and GRP in gastric cancer was correlated with lymph node metastasis (P< 0.05).</p><p><b>CONCLUSION</b>Tissue chip technique is a feasible,rapid,economic and accurate approach for screening clinical tissue specimens on a large scale.</p>