RESUMO
A rapid gas liquid Chromatographic [GLC] method for the determination of reserpine is proposed. The method depends on the trans-esterification of trimethoxybenzoic acid moiety in reserpine molecule with Methelute where the respective methyl ester is GLC monitored. The method succeeded in separating reserpine from the accompanying components in the pharmaceutical preparations and gave linear response curves. The method was successfully applied to the micro-determination of reserpine in various pharmaceutical preparations before and after the addition of standard reserpine
Assuntos
Química FarmacêuticaRESUMO
Methoxsalen [9 methoxypsoralen] National Reference Standard was prepared from locally produced bulk after due purification. The purity of the available bulk was assessed by various analytical techniques. Purification was carried out by selective elution from a silica gel column. The purified product was compared with the current lot of U.S.P. Methoxsalen Reference Standard using UV, IR, TLC, GLC, HPLC and melting point determination. As the purified substance proved to be comparable to the U.S.P. Standard, it was recognised as National Reference Standard
Assuntos
Cromatografia em Gel , Padrões de Referência , Química FarmacêuticaRESUMO
It has been reported [1, 2] in our laboratory that the furan ring undergoes cycloaddition reaction with halo-o-benzoquinones. The quinones added readily, under very mild conditions, to the 4,5-double bond of the furyl residue of 2-vinylfurans to give adducts of the type [2] with retention of the vinyl side chain