RESUMO
The aim of the study was to determine the effects of sesame seeds on anthropometric measurements [height, weight and body mass index], blood glucose, lipid profile and liver function tests In high fructose diet [HFD] fed mice. Arandomized experimental laboratory trial. The study was conducted at National Institute of Health Sciences, Islamabad from 1[st] February 2013 till 31st January 2014. We allocated 30 female Balb/c mice into three groups. Control Group I [n=10] mice who were fed with standard laboratory diet were compared with Experimental groups; Group Ha [n=10] mice were fed on high fructose diet [HFD] for 08 weeks, Group Mb: [n=10] mice were fed with HFD plus sesame meal for 08 weeks. Anthropometric measurements [Weight, Height and BMI] and serum lipid profile, liver function tests and blood glucose were measured at baseline and after 8 weeks. The mean weight of the Balb/c mice was 23.33 +/- 1.44 grams, the mean height was 8.4510.314 cm and the mean BMI was 3.27 +/- 0.33. The anthropometric measurement of the three groups of mice was similar at the baseline. After 8 weeks there was significant weight gain in the HFD group [Ma] 35.914.5 and HFD plus Sesame diet group [lib] 3014.5 as compared to control group 29.112.84. However the weight gain in HFD plus Sesame diet group [Mb] was significantly lesser as compared to the HFD alone group, signifying that perhaps sesame seeds prevented the significant weight. The mice that were fed on HFD [Ma] had significant derangement of their liver function tests, lipid profile and blood glucose as compared to control and HFD plus Sesame diet group [Mb]. High fructose diet results in significant weight gain, elevation of liver function tests, derangement of lipid profile and hyperglycemia. Sesame diet was effective in preventing these anthropometric and biochemical derangements. Hence it is likely that sesame diet has a hepato-protective role which needs to be confirmed by studies on a larger scale to demonstrate this hepatoprotective effect of sesame seeds beyond doubt
RESUMO
To determine the effect of cinnamon bark on glucose metabolism in streptozotocin induced diabetic mice. Interventional experimental study. This study was carried out in the animal house of National Institute of Health, Islamabad in collaboration with Department of Biochemistry, Islamic International Medical College from 7[th] November 2013 till 21[th] January 2014. Fifty albino Balb/C male mice were included in study. Among them, ten mice were used for cardiac puncture for baseline biochemical analysis. While in rest of the forty mice, Type 2 diabetes was induced by intraperitoneal administration of low dose [40 mg/kg] streptozotocin [streptozotocin] injections for four consecutive days. Diabetes induction was confirmed on day 21. Two groups with twenty mice each were made. Group I was control group that was left untreated while group II received cinnamon bark diet at a dose of 5mg/day for 8 weeks. At baseline the mean BGR was 135.9 +/- 29.67 mg/dl and day 21; BGR of 336.85 +/- 46.4 mg/dl. In the experimental group the mean BGR after 08 weeks of cinnamon therapy was 184.1 +/- 24.56 mg/dl; The BGR significantly decreased after 08 weeks of cinnamon therapy as compared to Day 21 BGR; p=0.00. The mean BGR at 08 weeks was significantly decreased between the control and experimental groups; p= 0.000. At baseline the HbA1c ranged from 5.5 to 6.2% with a mean of 5.93 +/- 0.20. In the Control group after 08 weeks the mean HbA1c was 11.27 +/- 1.28%. While in the experimental group the mean HbA1c after 08 weeks of cinnamon therapy was 7.7 +/- 0.68%. The mean HbA1c at 08 weeks was significantly decreased between the control and experimental groups; p= 0.000. Cinnamon therapy was effective in improving BGR and HbA1c levels in a diabetes type 2 induced male mice