RESUMO
The biosynthetic pigment from Chromobacterium violaceum BB-78, 1,3-dihydro-2H-indol-2-one and its derivatives exhibit biological activities such as antimicrobial action, low hemolytic effects on red blood cells and in vitro trypanocide activity. A relatively high cytotoxicity on V-79 hamster fibroblast cells of the biosynthetic pigment was found, although with the methylol derivative the toxicity was almost eliminated. The methylol derivative exhibited similar toxicity as Nifurtimox, a known, commercial trypanocide compound
Assuntos
Animais , Cricetinae , Antibacterianos/toxicidade , Chromobacterium/metabolismo , Hemólise , Indóis/toxicidade , Pigmentos Biológicos/toxicidade , Antibacterianos/isolamento & purificação , Bactérias/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Chromobacterium/crescimento & desenvolvimento , Fibroblastos/efeitos dos fármacos , Indóis/isolamento & purificação , Testes de Sensibilidade Microbiana , Nifurtimox/toxicidade , Pigmentos Biológicos/isolamento & purificação , Trypanosoma cruzi/efeitos dos fármacos , Trypanosoma cruzi/crescimento & desenvolvimentoRESUMO
It has been found that the pigment-I from Chromobacterium violaceum, 3 [1,2 dihydro 5 (5 hydroxy 1H indol 3 yl) 2 oxo 3H pyrrol-3 ylidiene] 1,3 dihydro 2H indol 2 one, has trypanocide activity. The formylated derivatives, pigment-III, immobilized 100% of the Trypanosoma cruzi at a level of 46 micronM after 48h of interaction with a total growth inhibition in the same period. Pigment I exhibited low toxicity and a DNA synthesis inhibition similar to that of Nifurtimox, a known trypanocide compound