RESUMO
<p><b>OBJECTIVE</b>To investigate the effects of red orpiment on cell morphology, expression of promyelocytic leukemia (PML) mRNA and its protein localization in NB4 and HL-60 cell lines.</p><p><b>METHODS</b>Cell morphology was assayed by Wright's staining and fluorescence staining, while PML mRNA expression was determined by RT-PCR. PML protein localization by evaluated by immunofluorescence staining.</p><p><b>RESULTS</b>The typical apoptosis was found in NB4 and HL-60 cells after treatment with red orpiment. The fusion protein was no longer observed in NB4 cells, PML protein was relocated, and then degraded. In HL-60 cells, PML protein underwent a similar progress. The expression of promyelocytic leukemia (PML) mRNA was not changed in the treated cells.</p><p><b>CONCLUSION</b>Red orpiment inhibits the proliferation of leukemia cells by inducing them to undergo apoptosis.</p>
Assuntos
Humanos , Antineoplásicos , Farmacologia , Apoptose , Arsenicais , Farmacologia , Células HL-60 , Leucemia Promielocítica Aguda , Tratamento Farmacológico , Metabolismo , Patologia , Medicina Tradicional Chinesa , Proteínas de Neoplasias , Genética , Proteínas Nucleares , Proteína da Leucemia Promielocítica , RNA Mensageiro , Fatores de Transcrição , Genética , Células Tumorais Cultivadas , Proteínas Supressoras de TumorRESUMO
<p><b>OBJECTIVE</b>To investigate PML gene and protein expression and localization in leukemia cell lines.</p><p><b>METHODS</b>Cell morphology was assayed by Wright and fluorescence stain, PML mRNA expression by RT-PCR, and PML protein localization by immunofluorescence.</p><p><b>RESULTS</b>(1) Differentiation was observed by morphology in NB4 and HL-60 cells after treatment with all-trans retinoic acid (ATRA) while K562 cells did not show. Apoptosis was found in each cell line after treatment with quercetin. (2) After treatment with ATRA, the fusion protein disappeared and PML protein resumed in NB4 cells, while in HL-60 and K562 cells there was no difference from control cells. After treatment with quercetin, the fusion protein disappeared in NB4 cells, then degraded, and so did in HL-60 cells and K562 cells. (3) The expression of PML mRNA had no change in all the three cell lines after treatment with ATRA or quercetin.</p><p><b>CONCLUSION</b>PML plays a role of differentiation and apoptosis induction in leukemia cells at the translational level. PML in POD plays a role of apoptosis induction and growth control of leukemia cells.</p>
Assuntos
Humanos , Divisão Celular , Relação Dose-Resposta a Droga , Regulação Neoplásica da Expressão Gênica , Células HL-60 , Células K562 , Leucemia , Genética , Metabolismo , Patologia , Proteínas de Neoplasias , Genética , Metabolismo , Proteínas Nucleares , Proteína da Leucemia Promielocítica , Quercetina , Farmacologia , RNA Mensageiro , Genética , Metabolismo , Fatores de Tempo , Fatores de Transcrição , Genética , Metabolismo , Tretinoína , Farmacologia , Células Tumorais Cultivadas , Proteínas Supressoras de TumorRESUMO
<p><b>OBJECTIVE</b>To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.</p><p><b>METHODS</b>Two cDNA probes were made from mRNA of untreated or arsenic sulfide treated NB4 cells. The cells were labelled with Cy3 or Cy5 fluorescence dyes individually, hybridized with cDNA microarray, and scanned for fluorescent intensity. The altered gene expression was screened through the analysis of difference in gene expression profile.</p><p><b>RESULTS</b>Thirty four genes related to apoptosis, cell cycle and others expressed different after the treatment of arsenic sulfide, 28/34 were up-regulated, 6/34 down-regulated.</p><p><b>CONCLUSION</b>ABC50, PNAS-2 and cyclin G(2) might take part in the process of NB4 cell apoptosis induced by arsenic sulfide.</p>