Effects and significance of gas explosion on expression of NF-kappaB and ICAM1 in lung tissue of rats / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the expression of NF-kappaB and ICAM-1 in the gas explosion wounded lung of rats and the relationship.</p><p><b>METHODS</b>Digoxin labeled NF-kappaB was used as probe. In situ hybridization was performed to detect the NF-kappaB mRNA. Immunohistochemistry was used to detect the expression of NF-kappaB and ICAM-1.</p><p><b>RESULTS</b>The levels of NF-kappaB mRNA, the expression of NF-kappaB and ICAM-1 in the wounded rats were significantly increased and reached their peak two hours after injury. Pathology of lung tissue showed that some crachea epithelium mucosae were desquamated; congestion, edema of trachea wall and infiltration of neutrophilic granulocytes were found; hemorrhage, edema and infiltration of lots of inflammatory cells were present in alveolus cells. Electron microscope showed that type I, especially type II alveolus epithelia had degeneration and desquamation.</p><p><b>CONCLUSION</b>The injury of gas explosion can activate NF-kappaB, which has close correlation with the acute injury to lung.</p>

A study on relationship between rat brain tissues hurts of the gas explosion and the expression of protein kinase CamRNA and c-fos gene / 中华劳动卫生职业病杂志

<p><b>OBJECTIVES</b>To explore relationship between rat brain tissues hurts of gas explosion and the expression of Protein Kinase C alpha mRNA.</p><p><b>METHODS</b>Build up rat hurt model of gas explosion. In Situ Hybridization (IDH) technique was used to test Protein Kinase C alpha mRNA. Immunohistochemical Assays (IHA) was used to determine c-fos gene protein.</p><p><b>RESULTS</b>Only a little a mount expression of PKC alpha mRNA and c-fos of the control group was detected. The expression of the cerebral cortex and hippocampus of PKC alpha mRNA 24 h, 48 h and the 48 h increased obviously, and the 48 h reached the peak of expression; (t = 4.12 P < 0.01). The expression of c-fos protein of the cerebral cortex and hippocampus started to increase obviously at 0.5 h and the 4 h reached the peak, then the strength lowered gradually and the expression level came back normal level on fifth day.</p><p><b>CONCLUSION</b>The anoxia of brain tissues due to the gas explosion may promote the expression of PKCamRNA, and PKCamRNA could regulate the expression of the gene of c-fos. Both PKCamRNA and the gene of c-fos are involved in harmful processes to the nerve cells.</p>