Clinical Significance of EBV-DNA Copy Number in EBV Positive Lymphoma Patients / 中国实验血液学杂志

OBJECTIVE@#To investigate the effect of EBV-DNA copy number on the prognosis of patients with EBV positive lymphoma.@*METHODS@#Clinical data of 109 patients diagnosed as EBV positive lymphoma in Tianjin Medical University Cancer Institute and Hospital from January 2010 to January 2020 were enrolled and analyzed retrospectively. Kaplan-Meier analysis was used for survival analysis, Log-rank was used to compare the clinical characteristics between the patients in different groups, and Cox regression was used for multivariate analysis.@*RESULTS@#Among the 109 patients with EBV-positive lymphoma, the medium age were 56 (range 15 to 83) years old. 29 patients at Ann Arbor stage I-II while 80 patients at stage III-IV. The average value of EBV-DNA was 1 023 510 IU/ml, 7 patients were higher than the average value, while 102 patients were lower. KM survival analysis showed that OS and PFS in patients with EBV-DNA above average level were shorter than those in patients with EBV-DNA below average level (OS: P=0.048, PFS: P=0.001), EBV-DNA copy number was a factor affecting the prognosis of patients. In addition, LDH level showed positive correlation with EBV-DNA copy number (r=0.650), which was also one of the factors affecting OS (P=0.053).@*CONCLUSION@#EBV-DNA copy number and LDH level can influence the prognosis of EBV positive lymphoma patients. Therefore, detection of EBV-DNA copy number in peripheral blood is important for evaluate the prognosis the patients.

Application of the national diagnostic criteria of occupational mercury poisoning / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the clinical manifestation of patients with renal injury induced by chronic mercury intoxication and the application of the diagnostic criteria of occupational mercury poisoning.</p><p><b>METHODS</b>The clinical data of 8 patients with chronic occupational mercury intoxication were analysed and evaluated.</p><p><b>RESULTS</b>All the observed clinical signs of chronic mercury intoxication correspond with the items of the diagnostic criteria of occupational mercury poisoning. The increasing beta2-MG was one of the clinical manifestations of renal injury induced by chronical mercury intoxication. The renal injury obviously was dose-dependent and reversible.</p><p><b>CONCLUSIONS</b>The national diagnostic criteria of occupational mercury poisoning is practically valuable. The renal injury induced by chronic mercury intoxication should not be neglected.</p>

Roles of immunohistochemistry and detection of SYT-SSX fusion gene in diagnosis of synovial sarcoma / 中华病理学杂志

<p><b>OBJECTIVE</b>To assess the diagnostic values of immunohistochemistry and SYT-SSX fusion gene detection for synovial sarcoma.</p><p><b>METHODS</b>Based on clinical features, histological and immunohistochemical profiles, 195 cases of tumors were divided into three diagnostic categories: definitive synovial sarcoma, probable synovial sarcoma and possible synovial sarcoma. RT-PCR Detection of the SYT-SSX fusion gene was performed using paraffin embedded tissue samples. Comparison between RT-PCR and immunohistochemistry results was carried out and their diagnostic value was evaluated.</p><p><b>RESULTS</b>There were 62 (31.8%) definite synovial sarcomas, 49 (25.1%) probable synovial sarcomas and 84 cases (43.1%) possible synovial sarcomas. SYT-SSX fusion gene was detected in 140 (78.2%) cases overall, including 94.7% (54/57) definite synovial sarcomas, 86.0% (37/43) probable synovial sarcomas and 62.0% (49/79) possible synovial sarcomas. In tumors in the certain and probable synovial sarcoma categories, the positive rates of epithelial membrane antigen (EMA) were significantly higher in the SYT-SSX positive cases than SYT-SSX-negative cases (P = 0.022, P = 0.010, respectively). EMA was positively correlated with the presence of SYT-SSX (r(s) = 0.431, P = 0.001, r(s) = 0.463, P = 0.002, respectively). However, such a correlation was not seen in cytokeratin (CK), vimentin or S-100 protein immunostains (P > 0.05). In tumors of possible synovial sarcoma category, there were no significant differences of CK, EMA, vimentin or S-100 protein between SYT-SSX-positive and SYT-SSX-negative tumors.</p><p><b>CONCLUSIONS</b>SYT-SSX fusion gene detection is not needed when the conventional approaches are diagnostic. EMA positivity has a similar diagnostic value to that of SYT-SSX by RT-PCR for tumors in the probable synovial sarcoma category. However, detection of SYT-SSX is very important for diagnosis of the tumors in the category of possible synovial sarcoma.</p>

Targeted down-regulation of p53 gene expression by individual antisense RNA in vitro / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate the effect of specific blockage of mutant p53 gene by individualized antisense RNA in vitro.</p><p><b>METHODS</b>Mutation status of p53 in human breast cancer cell lines was determined by immunocytochemical staining, PCR-SSCP and sequencing. Single strand antisense transcription system targeting specific p53 mutation site (mt-p53) was constructed, and corresponding antisense RNA was prepared. The hybridization of antisense RNA with its corresponding mt-p53 gene was confirmed by in-situ hybridization. Human breast cancer cells were transfected with antisense RNA by cationic liposome-mediated method. Time course of effects of antisense RNA was investigated by immunocytochemical staining and cell growth inhibiting assay. Expression of mt-p53 protein was examined by Western blot. Cell proliferation was evaluated by MTT assay and cell cycle distribution was determined by flow cytometry (FCM). Apoptosis was determined by TUNEL assay.</p><p><b>RESULTS</b>Mutation of p53 exon 8 was found in MDA-MB-231 cells and antisense transcription system (pGEM3zf (+/-) p53exon8) was then constructed successfully. In transfected MDA-MB-231 cells, hybridization signals were observed in cytoplasm. Fourth-eight hours after transfection, the antisense RNA (ASp53exon8'RNA) had a significant retarding effect on p53 related proliferation inhibition, along with a decrease of p53 protein expression.</p><p><b>CONCLUSIONS</b>ASp53exon8'RNA specifically blocks mt-p53 gene expression, resulting in an inhibition of MDA-MB-231 cell proliferation. Such an approach may be used as a therapeutic option against human malignancy.</p>

Prognostic molecular classification of breast cancers based on gene expression profiling / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To screen a set of gene markers related to metastasis and prognosis of breast cancer by comparison of gene expression profiles of primary breast cancers with distant metastasis to the cases without distant metastasis within 3 years follow-up, and to explore the clinical significance of those gene expression in prognostic molecular classification of breast cancer patients.</p><p><b>METHODS</b>5 cases with distant metastasis and 5 cases without distant metastasis within 3 years follow-up were used as training cases to compare their gene expression profiles by Oligo microarray hybridization containing 21 329 human functional genes. K-mean supervised cluster was done for 10 training cases and additional 20 testing cases based on the set of differential genes. "Leave-one-out" was used to eliminate useless genes to obtain optimal gene set that was used for prognostic molecular classification of breast cancer patients.</p><p><b>RESULTS</b>The different genes screened out from gene expression profiling of primary breast cancers with and without distant metastasis could classify breast cancer patients into two sub-groups. All patients with distant metastasis were included in the "poor prognosis group" (7/10), whereas there were no case showing distant metastasis in the "good prognosis group" (0/20), with a statistically significant difference by exact probability test (P =0. 03). In the set of 104 optimal genes, all 5 genes involved in cell adhesion and migration were up-regulated in cases with distant metastasis, all 2 genes related to immune response of host were down-regulated, 11 genes related to cell growth and metabolism were up-regulated and 14 down-regulated, and 15 genes related to cell signal transduction were significantly changed.</p><p><b>CONCLUSION</b>A set of genes involved in cell adhesion and migration, cell growth and metabolism, immune response mechanism, cell signal transduction were screened out by comparing gene expression profiles of primary breast cancers with and without distant metastasis within 3 years follow-up, showing highlight in prognostic molecular classification of breast cancer patients and hopeful would benefit to choose patient-tailored therapy strategies.</p>

Clinical significance and relationship of expression of matrix metalloproteinase-2 mRNA with breast cancer metastasis / 中华外科杂志

<p><b>OBJECTIVE</b>To discuss the clinical significance of matrix metalloproteinase-2 (MMP-2) mRNA and the relationship of MMP-2 mRNA expression with breast cancer metastasis.</p><p><b>METHOD</b>Detect MMP-2 mRNA expression of 30 breast cancer, metastatic lymphnode and 116 clinical breast cancer samples by flurence-quantitative RT-PCR and analysis the relativity of MMP-2 mRNA expression with clinical, pathology factors.</p><p><b>RESULTS</b>MMP-2 mRNA expression in 30 metastatic lymphnode is lower than in primary breast cancer (t = -3.293, P < 0.05). The mRNA expression of MMP-2 in tumor > 2 cm is lower than in tumor </= 2 cm (t = 1.936, P < 0.05). The MMP-2 mRNA expression in stage II, III is lower than in stage I (t = 2.466, P < 0.05). The mRNA expression of MMP-2 in more than 4 lymphnodes positive is lower than in lymphnode 1 - 3 positive (t = 3.202, P < 0.05). The MMP-2 mRNA expression is irrelated with tissue grade, pathologic type, ER, PR and Her-2.</p><p><b>CONCLUSIONS</b>MMP-2 mRNA expression is up-regulated in early clinic stage, lymphnode metastatic early stage, and down regulated with tumor progressing. MMP-2 mRNA expression is related with the metastasis of breast cancer.</p>