A new flavonoid isolated from fruits of Solanum virginianum / 中草药

Objective: To study the chemical constituents from the fruits of Solanum virginianum. Methods: By means of preparative HPTLC and column chromatography over silica gel and Sephadex LH-20, compounds were isolated and purified. Their structures were elucidated by physico-chemical properties and spectral analyses. Results: Twelve compounds were obtained and identified as: 5-hydroxy-8-methoxy-6,7-methylenedioxyflavone (1), 7-hydroxy-6-methoxy coumarin (2), fraxetin (3), 5-hydroxy- 6,7,3',4'-tetramethoxyflavone (4), 5-hydroxy-4',6,7-trimethoxyflavone (5), dihydro-N-feruloyltyramine (6), N-trans-coumaroyltyramine (7), 5,3'-dihydroxy-6,7,4'-tritermethoxyflavone (8), N-[2-(3,4-dihydroxyphenyl)-2-hydroxyethyl]-3-(4-methoxyphenyl)-prop-2-enamide (9), N-trans-coumaroyloctopamine (10), 5,7,4'-trihydroxy-6-methoxyflavone (11), and 5,7,4'-trihydroxy-8-methoxyflavone (12). Conclusion: Compound 1 is a new flavonoid, named as solacarpumon, and compounds 2-12 are isolated from Solanum virginianum for the first time.

Network pharmacological analysis and mechanism prediction of Xingnaojing Injection in treatment of neurological damage caused by SARS-CoV-2 / 中草药

Objective: To explore the active compounds, targets and signaling pathways of Xingnaojing Injection (XNJI) for the treatment of neurological damage caused by SARS-CoV-2, so as to explore its mechanism. Methods: Using TCMSP, BATMAN, Swiss Target Prediction, and other databases, the chemical compounds and targets of XNJI were retrieved. Cytoscape software was used to construct XNJI efficacy network of "drug-compounds-targets" for coronavirus and neuroprotection, and the action mechanism was predicted by Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Then core compounds were verified by molecular docking with 3CL Mpro, ACE2, and 2019-nCoV RBD/ACE2-B0AT1 complex. Results: A total of 105 active compounds of XNJI, 928 drug targets, 741 targets of coronavirus, 611 targets of neuroprotection, 83 drug-disease common targets, 12 core compounds, and seven key targets were obtained. The function enrichment analysis of GO yielded 204 entries, KEGG pathway enrichment screened 120 signaling pathways, which included Hepatitis B, pathways in cancer, TNF, HIF-1, and VEGF signaling pathway, and so on. The results of molecular docking showed that core compounds of XNJI had a good bonding activity with 3CL Mpro, ACE2 and complex. The chlorogenin and kaempferol had the lowest binding energy with three proteins and might play an important role in treatment. Conclusion: The core compounds in XNJI including chlorogenin, kaempferol, 5-hydroxy- 6,7,3',4',5'-pentamethoxyflavone, 3-methylkempferol, morin, gardenin, quercetin, artemisetin, genistein, dryobalanone, curcumin, and elemicin, which might interfere with various signaling pathways by acting on key targets like PARP1, PTGS2, MMP9, CDK2, ADORA2A, ALOX5, GSK3B, and regulate the inflammatory response, apoptosis, oxidative stress, angiogenesis, and other processes to improve the neurological damage caused by SARS-CoV-2, and inhibit virus replication and prevent infection of the host cell by binding with 3CL Mpro, ACE2 and complex, which suggest that XNJI may have a positive therapeutic effect on the neurological damage caused by SARS-CoV-2.

Chemical constituents from stems and leaves of semi-mangrove plant, Barringtonia racemosa / 中草药

Objective: To study the chemical constituents of the stems and leaves of semi-mangrove plant, Barringtonia racemosa. Methods: The chemical constituents of B. racemosa were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literature. Results: A total of 17 compounds were isolated from the 90% ethanol extract of the stems and leaves of B. racemosa, which were identified as chrysin (1), ayanin (2), genkwanin (3), rhamnocitrin (4), tricin (5), dillenetin (6), 5,3'-dihydroxy-7,4'-dimethoxyflavone (7), 5,7,3',4'-tetramethoxyflavone (8), 5-hydroxy-6,7,8,3',4'- pentamethoxy flavone (9), petasitolone (10), sarmentol F (11), dehydrovomifoliol (12), blumenol A (13), 10-hydroxyaristolan-9-one (14), alphitolic acid (15), oleanolic lactone (16), and 11,12-dehydroursolic acid lactone (17). Conclusion: All compounds are isolated from the genus Barringtonia for the first time.

A new aurone glycoside from Bidens parviflora / 中草药

Objective: To study the chemical constituents of Bidens parviflora. Methods: The ethyl acetate fraction of 80% ethanol extract from B. parviflora was isolated and purified by silica, polyamide, Sephadex LH-20, and HPLC, then the structures of obtained compounds were identified by physicochemical properties and spectral data. Results: Ten compounds were isolated and identified as Z-6-O-(4″-O-acetyl-6″-O-p-coumaroyl-β-D-glucopyranosyl)-6,7,3’,4’-tetrahydroxyaurone (1), okanin-4’-O-β-D-(6″-O- acetyl)-glucoside (2), Z-6-O-(4″,6″-diacetyl-β-D-)-7,3’,4’-tetrahydroxy aurone (3), 6,7,3’,4’-tetrahydroxy aurone (4), isookanin (5), syringic acid-4-O-α-L-rhamnopyranoside (6), okanin-4’-O-β-D-(6″-trans-p-coumaroyl)-glucoside (7), okanin-4’-O-β-D-(4″-acetyl- 6″-trans-p-coumaroyl)-glucoside (8), quercetin-3,4’-dimethyl ether-7-O-rutinoside (9), and cordifolioidyne B (10). Conclusion: Compound 1 is a new compound named as bidenoside I, compounds 6 and 10 are isolated from the genus Bidens for the first time, other compounds are isolated from this plant for the first time.

Isolation of chemical ingredients from Aster ageratoides and their anticomplement and anti-inflammatory activities / 中草药

Objective To isolate and identify chemical ingredients with anti-complement activity from Aster ageratoides and investigate the key targets and anti-inflammatory activities of obtained compounds with good anti-complement activity. Methods Using silica gel column, sephadex LH-20 column, Medium Pressure Liquid Chromatography system, and Semi-preparative HPLC, chemical ingredients that displayed anti-complement activity were isolated. Their chemical structures were identified by 1H-NMR and 13C-NMR and their anti-complement activities and targets were investigated by erythrocyte hemolysis in vitro. In addition, using LPS-stimulated RAW264.7 cells, we investigated the anti-inflammatory activity of compound 2. Results A total of 14 compounds were obtained from A. ageratoides and identified as oleanolic acid (1), quercetin (2), kaempferol (3), 3,5,7,3’-tetrahydroxy- 4’-methoxyflavone (4), kaempferol-7-O-α-L-rhamnopyranoside (5), quercetin-3-O-β-D-glucopyranoside (6), kaempferol-3-O-α-L- rhamnoside (7), quercetin-3-O-α-L-rhamnoside (8), kaempferide-3-O-β-D-glucopyranoside (9), kaempferol-3-O-β-D- glucopyranoside (10), kaempferol-7-O-β-D-glucopyranoside (11), kaempferol-3-O-β-D-glucopyranoside-7-O-β-D-glucopyranoside (12), kaempferide-3-O-α-L-rhamnoside-(1→6)-β-D-glucopyranoside (13), and rutin (14). They all exhibited anti-complement activity to some certain degree and good structure-activity relationship. The targets of compounds 1 and 2 were C1q, C5, and C9 and C1q, C2, C5, and C9, respectively. The anti-inflammatory experiments indicated that compound 2 exhibited a significant biological activity, which significantly suppressed the release of NO, TNF-α, and IL-6 and expressions of iNOS and COX-2. Conclusion A total of 14 compounds were obtained and they all displayed anticomplement activity, of which compounds 1, 4, 6, 9, 12, and 13 are firstly discovered in A. ageratoides. Compound 2 exhibited a significant anti-inflammatory activity.

A new iridoid glycoside from leaves of Callicarpa nudiflora / 中草药

Objective To study the chemical constituents of the leaves of Callicarpa nudiflora. Methods The chemical constituents were isolated and purified by column chromatography on silica gel, MPLC, and PHPLC. Their structures were elucidated on the basis of physicochemical properties and spectroscopic analysis. Results Five compounds were isolated from the leaves of C. nudiflora and elucidated as 6’-O-caffeoyl-ajugol (1), luteolin (2), 5,4’-dihydroxy-3,7,3’-trimethoxyflavone (3), luteolin-4’-O-(6’’-E-caffeoyl)- β-D-glucopyranoside (4), and 2α,3α,19α,23-tetrahydroxyurs-12-en-28-ursolic acid (5). Conclusion Compound 1 is a new compound named nudifloside A1.

Chemical constituents from mangrove plant Sonneratia paracaseolaris / 中草药

Objective: To study the chemical constituents of the methanol extract from the aerial parts of the mangrove plant Sonneratia paracaseolaris. Methods: The methanol extract was isolated and purified with various chromatographic methods, including silica gel, ODS, Sephadex LH-20 columns, TLC, and HPLC. The compounds were identified by their physical chemical properties and 1H-NMR and 13C-NMR data. Results: Seventeen compounds were obtained from the methanol extract of Sonneratia paracaseolaris and identified as phytol (1), stigmasta-4-ene-3,6-dione (2), stigmata-4,22-diene-3,6-dione (3), cholesterol (4), (22E)-cholesterol-5,22-diene-3β-alcohol (5), diosmetin (6), tricin (7), 5,3′,5′-trihydroxy-7,4′-dimethoxyflavone (8), 5-hydroxy- 7,4′-dimethoxyflavone (9), 5-hydroxyl-7,3′,4′-trimethoxydihydroflavone (10), vanillin (11), p-hydroxy benzaldehyde (12), salicylic acid (13), trans-p-hydroxyl ethyl cinnamate (14), 4-hydroxy-2,6-dimethoxy-benzaldehyde (15), 3,4,5-trimethoxybenzoic acid (16), and 3,3′,4-trimethoxyellagic acid (17). Conclusion: All the compounds except 4, 11, 15, and 17 are obtained from genus Sonneratia. All compounds are isolated from S. paracaseolaris for the first time.

Chemical constituents from leaves of Camellia nitidissima var. longistyla (II) / 中草药

Objective To investigate the chemical constituents in the root of Camellia nitidissima var. longistyla. Methods The chemical constituents were separated and purified by silica gel, sephadex LH-20 column chromatography, preparative HPLC and so on. Their structures were determined by physical constants and spectral analyses. Results Sixteen compounds were obtained and identified as kaempferol (1), 3,5,6,7,4'-pentahydroxyflavone (2), 6-hydroxykaempferol (3), (-)-epiafzelechin (4), (-)-epicatechin (5), kaempferol-3-O-β-D-glucopyranoside (6), α-spinasterol (7), β-daucosterol (8), 5,7,3',4'-tetramethoxyflavone (9), 7,3',4'-trimethoxy-5- hydroxyflavone (10), 5,7,3',4',5'-pentamethoxyflavone (11), 3-O-acetyl oleanolic acid (12), n-tetratriacontanol (13), β-amyrin (14), α-spinasterol-3-O-β-D-galactopyranoside (15), and rutin (16). Conclusion All compounds are isolated from the plant for the first time. Compounds 2, 3, and 9-11 are isolated from the plants of Camellia for the first time.

Chemical constituents from seeds of Aesculus chinensis / 中草药

Objective: To study the chemical constituents from the 70% ethanolic extracts of Aesculus chinensis seeds. Methods: The compounds were isolated by D101 macroporous adsorptive resins, silica gel, Sephadex LH-20, and preparative-HPLC and their structures were identified by chemical methods and spectroscopic analyses. Results: Ten compounds were isolated from the 70% ethanolic extracts of A. chinensis seeds. They were 2R,3R-3,7,3'-trihydroxy-5'-methoxyflavane-5-O-β-glucopyranoside (1), quercetin-4'-O-β-D-glucopyranoside (2), quercetin-3'-O-β-D-glucoside (3), quercetin-3-O-[β-D-xylopyranosyl (1-2)]-β-D- glucopyranoside (4), quercetin-3-O-[β-D-glucopyranosyl (1-4)]-α-L-rhamnopyranoside (5), kaempferol-3-O-[β-D-glucopyranosyl (1-4)]-α-L-rhamnopyranoside (6), kaempferol-3-O-[β-D-xylopyranosyl (1-2)]-β-D-glucopyranoside (7), kaemferol-3-O-[β-D- xylopyranosyl (1-2)] [β-D-glucopyranosyl (1-3)]-β-D-glucopyranoside (8), (-)-epicatechin (9), and quercetin (10). Conclusion: Compounds 1 and 2 are obtained from genus Aesculus Linn. for the first time, and compounds 3-9 are isolated from A. chinensis for the first time.

HPLC fingerprint analysis and content determination of Gleditsiae Spina / 中草药

Objective: An HPLC method was developed to evaluate the quality of Gleditsiae Spina through three components determination and fingerprint analysis. Methods: The assay was performed on Waters symmetry C18 (250 mm × 4.6 mm, 5 μm) column. The mobile phase consisted of acetonitrile and 0.1% methanoic acid in water with gradient elution by PDA detection at 338 nm. Results: Thirteen batches of Gleditsiae Spina were analyzed with the established method. In the fingerprint, 14 common peaks were marked and three of them were determined. The three compounds showed good linearity (r ≥ 0.999 2) in the range of 0.091 3-5.840 0, 0.176 3-11.280 0, and 0.014 0-0.895 0 mg/mL, respectively. The average recoveries were 98.97%-99.66% with RSD < 2.5%. Conclusion: This method has good repeatability and stability and provides a new method for the quality control of Gleditsiae Spina.

Flavonoids from Gleditsiae Spina and their antitumor activities / 中草药

Objective: To investigate the flavonoids from Gleditsiae Spina (thorns of Gleditsia sinensis) and their antitumor activities. Methods: The chemical constituents in the EtOAc fraction from Gleditsiae Spina were isolated and purified by the chromatography on silica gel, Sephadex LH-20 columns, and semi-preparative HPLC. Their structures were identified by various spectroscopic analyses, and the cytotoxicity of compounds 7-16 were evaluated in vitro against MCF-7 cell lines by SRB method. Results: Sixteen compounds were isolated from the extracts of Gleditsiae Spina and identified as tricin (1), liquiritigenin (2), 7,4'-dihydroxy-5,3'-dimethoxyflavanonol (3), garbanzol (4), 7,3',5'-trihydroxyflavanone (5), 7,4'-dihydroxyflavonol (6), dihydrokaempferol (7), butein (8), (2S)-5,7,3',5'- tetrahydroxyflavanone (9), 7,3',5'-trihydroxy-5-methoxyflavanonol (10), quercetin (11), fustin (12), fisetin (13), leucorobinetinidin (14), thevetiaflavon (15), and isovitexin (16). Compound 8 showed the inhibitory effect against MCF-7 cells with IC50 value of 28.53 μmol/L. Conclusion: Compounds 1-6, 8-10, 14 and 15 are isolated from the plants of Gleditsia L. for the first time, compound 8 shows the significant cytotoxic activity against MCF-7 cells.

Saurusine B: A new lignan from Saururus chinensis / 中草药

Objective: To study the chemical constituents in the whole plants of Saururus chinensis. Methods: The chemical constituents were isolated by various column chromatographic methods. The structures of the compounds were elucidated on the basis of physiochemical properties and spectrascopic analysis. Results: Seven compounds were obtained and identified as trans-7, 8-dihydro-7-(3,4-methylenedioxyl)-phenyl-1'-(2-oxopropyl)-3'-methoxy-8-methylbenzofuran (1), 4-(3-methoxy-4-hydroxy) phenyl-3- methyl-3-buten-2-one (2), (+)-guaiacin (3), (+)-trans-1,2-dihydrodehydroguaiaretic acid (4), (-)-isolariciresinol-4-O-β-D-glucoside (5), isonectandrin B (6), and perseal F (7), respectively. Conclusion: Compound 1 is a new compound named as saurusine B, and compounds 2-7 are obtained from this plant for the first time.

Analysis on chemical constituents in Bupleuri Radix by HPLC-Q-TOF-MS / 中草药

Objective: To investigate the chemical constituents in extract of Bupleuri Radix using HPLC-Q-TOF-MS. Methods: The HPLC separation achieved on Agilent 1200 HPLC Diamonsil II C18 column (250 mm×4.6 mm, 5 μm) was used with a mobile phase of 0.05% H2O-formic acid (A) and acetonitrile (B), the flow rate was 1.0 mL/min, the column temperature was 35℃, and the detection wavelength was 200-600 nm. Positive and negative ions MS information and element analysis, via comparing MS data with those of the standard compounds and coupled with the related literature were used to analyze the main chemical constituents of BupleuriRadix. Results: Twenty-one chemical constituents were identified, including three phenolic acid, four flavonoids, and fourteen triterpenoid saponins, and isochlorogenic acid A (5), isochlorogenic acid B (6), 7,3'-di-O-methylquercetin (8), and 5-hydroxy-7-acetoxyflavone (24) were separated from Bupleuri Radix for the first time. Conclusion: It is used to quickly analyze the chemical constituents from Bupleuri Radix by establishing a rapid accurate evaluation method using HPLC-Q-TOF-MS, which could provide the references for the application development.

Flavonoids from roots and rhizomes of Rhodiola crenulata / 中草药

Objective: To study the chemical constituents from the roots and rhizomes of Rhodiola crenulata. Methods: The chemical constituents were isolated by repeated silica gel chromatography and medium pressure column chromatography. Their structures were identified by various spectroscopic data including ESI-MS, 1H-NMR, and 13C-NMR data. Results: Fourteen compounds were isolated from the ethyl acetate fractions of R. crenulata including 3,5-dihydroxy-3',4',7-trimethoxyflavone (1), 3,5,7,3'-tetrahydroxyflavone (2), 5,4'-dihydroxy-7,3'-dimethoxyflavone (3), kaemnpferol (4), kaemnpferol-3-O-β-D-glucopyranoside (5), kaemnpferol-3-O-α-L- rhamnopyranoside (6), tricin (7), tricin-7-O-β-D-glucopyranoside (8), quercetin (9), quercetin-3-O-β-D-glucopyranoside (10), quercetin-3-O-α-L-rhamnopyranoside (11), herbacetin-3-O-β-D-glucopyranoside (12), herbacetin-7-O-β-D-glucopyranoside (13), and herbacetin-7-O-α-L-rhamnoside (14). Conclusion: Compounds 1-3 are isolated from the plants in Rhodiola L. for the first time, compounds 5-6, 8, 10-13 are obtained from this plant for the first time.

Chemical constituents from Scutellaria barbata / 中草药

Objective: To investigate the constituents in the aerial parts of Scutellaria barbata. Methods: The isolation and purification of the compounds were performed by AB-8 macroporous adsorption resin, silica gel, polyamide and Sephadex LH-20 column chromatography, and their structures were determined on physicochemical characters and spectroscopic data. Results: Fourteen compounds were separated and elucidated as hispidulin-7-O-β-D-methylgluzcuronide (1), apigenin (2), scutellarin (3), scutellarein (4), luteolin (5), scutellarein-7-O-β-D-glucuronide methyl ester (6), isoscutellarein-8-O-β-D-glucuronide-6″-methyl ester (7), apigenin-7-O-β-D-glucuronide-6″-methyl ester (8), 4'-hydroxywogonin (9), 4',5-dihydroxy-3',5',6,7-tetramethoxyflavone (10), isoscutellarein (11), 6-hydroxyluteolin (12), 5-hydroxy-6,7,3',4'-tetramethoxyflavone (13), salvigenin (14). Conclusion: Compound 7 is isolated from Lamiaceae for the first time, compounds 1 and 13 are for the first time isolated from the genus Scutellaria, and compounds 6 and 12 are for the first time obtained from S. barbata.

Studies on non-alkaloid constituents from Alstonia mairei / 中草药

Objective: To study the non-alkaloid constituents from the stems and leaves of Alstonia mairei. Methods: The chemical constituents were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties, spectral data, as well as comparisons with the data in literature. Results: Eighteen compounds were isolated from the petroleum ether fraction of 90% ethanol extract from the stems and leaves of A. mairei, and identified as lupeol (1), 30-oxo-lupeol (2), lupenyl acetate (3), α-amyrin (4), α-amyrenone (5), 23-hydroxyursolic acid (6), β-amyrin (7), β-amyrenone (8), maslinic acid (9), friedelinol (10), friedelin (11), tectochrysin (12), 5,6-dihydroxy-7,4'- dimethoxyflavone (13), 5,3'-dihydroxy-7,4'-dimethoxyflavone (14), 7-hydroxy-7,5,3',4'-trimethoxyflavone (15), 5,7,3',4'- tetramethoxyflavone (16), 5-hydroxy-6,7,8,4-tetramethoxyflavone (17), and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (18). Conclusion: All compounds are isolated from A. mairei for the first time. In addition to compound 1, the other compounds are isolated from the plants of Alstonia R. Br. for the first time.

Chemical constituents from Dianthus superbus / 中草药

To investigate the chemical constituents of Dianthus superbus. The chemical constituents were isolated from 95% alcohol extract of D. superbus by silica gel column chromatography, Sephadex LH-20 column chromatography, and HPLC. Their chemical structures were elucidated on the basis of 1D NMR and ESI-MS spectroscopic analyses. Nine compounds including three flavanoids, two sterols, three triterpenes, and one γ-lactone were isolated. Their structures were identified as 5-hydroxy-7, 3', 4'-trimethoxyflavanone (1), 5, 3'-dihydroxy-7, 4'-dimethoxyflavanone (2), 5, 4'-dihydroxy-7, 3'-dimethoxyflavanone (3), β-spinasterol (4), sterculin A (5), (24R)-cycloart-25-ene-3β, 24-diol (6), (24S)-cycloart-25-ene-3β, 24-diol (7), stigmast-7-en-3β-ol (8), and hydoxydihydrobovolide (9). Conclusion All the compounds (1-9) are isolated from D. superbu for the first time.

Chemical constituents of Artemisa sacrorum ledeb / 中国药学杂志

OBJECTIVE: To study the chemical constituents of the chloroform extract from the aerial parts of Artemisa sacrorum. METHODS: The chemical constituents were isolated and purified by silica gel and LH-20 column chromatography and preparation HPLC. Their structures were identified by spectral analysis methods. RESULTS: Thirteen compounds were obtained and identified as 5-hydroxyl-7, 4'-dimethoxyflavone(1), 4-hydroxylacetophenone(2), 5, 4'-dihydroxyl-7, 3'-dimethoxyflavone(3), 5, 7-dihydroxyl-6, 4'-dimethoxyflavone(4), 5, 7-dihydroxyl-6, 4'-methoxyflavone(5), 5, 4'-dihydroxyl-7-methoxyflavone(6), caffeic acid(7), 8-hydroxyl-6, 7-dimethoxycoumarin(8), 3, 4-dihydroxylbenzoic acid (9), acetophenone-4-O-β-D-glucoside(10), 6-methoxycoumarin-7-O-β-D-glucoside(11), 6, 8-dimethoxycoumarin-7-O-β-D-glucoside(12), and 2-hydroxyl-6-methoxyacetophenone-4-O-β-D-glueoside(13). CONCLUSION: Compounds 3, 4, 5, 9, 10 and 12 are isolated from this plant for the first time.

PHYTОCHEMICAL RESEARCH OF GENTIANA BARBATA / Монголын эм зүй, эм судлал

Introduction It grows a larch forest, in its meadow, edge and glade, bank of the river and thick bushes forests covering Khuvsgul, Khentii, Khangai, Gobi Altai, mountainous and fertile pastures, Mountain range and Mongol Altaic Mountain range. Upper part of the land of Gentiana barbata, its preparation is effective to release poisoning, reduce inflammation, support liver and gall procedure internal treatment and an application external are inflammation fast healing, recover transfer activities of cell membrane. Drug preparations extracted from upper part of the land is confirming cell membrane, to jaundice. Its upper part of the land are for fast healing scald and inflammation, gallbladder diseases, to jaundice in Mongolian traditional Medicine. Goal The aim is to study of phytochemical Gentiana barbata. Materials and MethodsWe performed the research taking material assistance of pharmacognozy laboratories in Monos university from 2014-2015. The quantitative and qualitative analyses of plant bioactive compounds were evaluated by Russian XI Pharmacopeia and the method according to Mongolian National Standard, mineral substances were evaluated using roentgen fluorescence method. Result of the study was developed by SPSS 16 and End notе programme.ResultThe quantitative and qualitative analyses of plant bioactive compounds of Gentiana barbata upper part of the land were evaluated alkaloid, tannin, coumarone, flavonoid and mineral substances were found. Conclusion It was carried out phytochemical investigation of the Gentiana barbata using spectrophotometer as a result we determined that contains 6.0 % of sum flavonoid, 15.7 % coumarone. Amount of biological active compound-tannins was 3.2 %, alkaloid 0.5 %. When content of mineral substances in upper part of the land of Gentiana barbata contained K 60.2 %, Ca 25.12%, S 6.7%, CL 5.8 %, Sr 0.09%, Fe 0.83%, Ba 0.73%, Mn 0.152%, Rb 0.058%, Cu 0.069%, Zn 0.207%.Key words: Gentiana barbata, phytochemical, mineral substances, flavonoid, and 5,7,3´-Trihydroxy-4´-methoxyflavon.

Content Determination of Heptanone in Mongolian Medicine Compound Shudage-4 by HPLC / 中国药房

OBJECTIVE:To establish a method for the content determination of 1-phenyl-7-(3-methoxy-4-hydroxy) phe-nyl-5-ol-3-heptanone in active ingredients of Mongolian medicine compound shudage-4. METHODS:HPLC was performed on the column of Intersil ODS-3 with the mobile phase of acetonitrile-0.5%phosphoric acid at the flow rate of 1.0 ml/min,the detection wavelength was 210 nm,temperature was 30 ℃,and the volume was 20 μl. RESULTS:The linear range of 1-phenyl-7-(3-me-thoxy-4-hydroxy) phenyl-5-ol-3-heptanone was 0.102-1.02 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.52%;average recovery was 97.10%(RSD=1.80%,n=6). CONCLUSIONS:The method is accurate,sim-ple and reproducible,and can provide basis for the quality control of Mongolian medicine compound shudage-4.