In vitro establishment and multiplication of Echynochloa polystachya

The objective of this work was to carry out the in vitro establishment of Echynochloa polystachya aiming at obtaining a micropropagation protocol for works involving the selection of superior genotypes and the cultivation of the species. E. polystachya stems were collected in the municipality of Manaus-AM. Explants were inoculated in test tubes containing Murashige and Skoog (MS) medium. Thirty days after in vitro establishment, the rate of sprouting and contamination were evaluated. Experiments were also carried out to assess the effects of sucrose and 6-benzylaminopurine (BAP) concentrations on the tillering rate of explants. It was found that during the successive subcultures there was a decrease in internodes and the consequent loss of vigor. There were responses in the multiplication rate at concentrations starting from 45 g L-1 sucrose. In addition, BAP and sucrose interfered the development and in vitro multiplication. Sucrose in conjunction with BAP was harmful and shortened internodes. The physiological state of the explants for the species under study was intrinsically linked to the concentrations of sucrose used for the culture medium and the concentrations of BAP. However, the sucrose and BAP concentrations suggested for in vitro cultivation of E. Polystachya must be adjusted during successive subcultures. Absence of contamination in the in vitro establishment occurred at concentrations 15, 30 and 60 g L-1 sucrose. The combination of 1.5 mg L-1 BAP and 30 g L-1 sucrose promoted greater induction of sprouts. In addition, the in vitro rooting of E. polystachya was 45%.

Cutaneous Larva Migrans With Bullous Eruptions in A Young Child

@#Infection of cutaneous larva migrans is usually acquired in the tropical region and mainly affects children. It is generally diagnosed with symptoms of severe itchiness and skin lesion that appears as a migrating erythematous linear lesion. It often occurs with a history of skin exposure to soil. Atypical presentation of the infection causes difficulty in diagnosis and delays treatment for the patient. Once diagnosed, cutaneous larva migrans is treated easily with helminth medications such as albendazole. Recognition of the skin lesion of cutaneous larva migrans in the atypical presentation of the infection for prompt treatment will comfort and prevent secondary infection complications. This case describes a presentation of bullous eruption in a child infected with cutaneous larva migrans.

Correlation analysis between mesenteric creeping fat index and inflammatory intestinal stricture in Crohn disease / 中华放射学杂志

Objective:To develop a mesenteric creeping fat index (MCFI) based on CT enterography (CTE) to characterize the degree of creeping fat wrapping around the inflamed gut in Crohn disease (CD), and to assess the relationship between MCFI and the inflammatory intestinal stricture.Methods:From December 2018 to July 2019, the patients with CD who underwent surgery in the First Affiliated Hospital of Sun Yat-Sen University were prospectively collected. The extent of perienteric mesenteric vessels wrapping around the gut was reconstructed to develop MCFI based on CTE images. The intestinal stricture index was obtained by calculating the ratio of the maximal upstream luminal diameter divided by the minimum luminal diameter apparent within the stricturing region. Using region-by-region correlation between CTE and surgical specimen, creeping fat score in intestinal specimen was obtained by assessing the extent of creeping fat wrapping around the resected bowel segment, and HE staining was performed on the bowel specimen corresponding to creeping fat to obtain the pathological inflammatory score. The Spearman correlation analysis was used to evaluate the correlation between MCFI, creeping fat score in intestinal specimen, and inflammatory score, intestinal stricture index. The ROC curve analysis was used to assess the accuracy of MCFI in distinguishing moderate-severe and mild inflammatory bowel walls.Results:Totally 30 CD patients were enrolled. The creeping fat score in intestinal specimen positively correlated with pathological inflammatory score ( r s=0.403, P=0.027) and with intestinal stricture index ( r s=0.642, P<0.001). MCFI positively correlated with creeping fat score in intestinal specimen ( r s=0.840, P<0.001), with pathological inflammatory score ( r s=0.497, P=0.005), and with intestinal stricture index ( r s=0.599, P<0.001). ROC analysis showed that the area under the curve of MCFI for differentiating moderate-severely from mildly inflammatory bowel walls was 0.718 (95%CI 0.522-0.913). Using MCFI≥4 as a cutoff value, the sensitivity and specificity were 81.8% and 47.4%, respectively. Conclusions:There was a correlation between creeping fat and inflammatory intestinal strictures in CD. MCFI can non-invasively depict the degree of creeping fat wrapping around the gut and assess the inflammatory intestinal stricture.

Reservas de carbono del pasto Cenchrus clandestinus (Poaceae) en los sistemas de manejo tradicional y silvopastoril, en diferentes relieves

El aumento de las emisiones de Gases de Efecto Invernadero (GEI) derivadas de las actividades humanas, son consideradas el principal responsable del cambio climático actual y el sector ganadero es responsable del 18 % de las emisiones de GEI en CO2 equivalente. El pasto kikuyo puede optimizar tanto la captura como la fijación del carbono. El objetivo del trabajo fue identificar las existencias de carbono en el pasto kikuyo en sus diferentes compartimentos, biomasa aérea (BA) y biomasa radicular (BR), a 20 y 40 cm de profundidad del suelo, bajo los sistemas tradicional y silvopastoril en diferentes relieves. Se realizaron seis muestreos (M) sucesivos de acuerdo al sistema de pastoreo (tradicional y silvopastoril), la geoforma del terreno (flanco cóncavo (FCC), flanco convexo (FCX), flanco rectilíneo (FR) y relieve plano (RP)). Se muestrearon la biomasa aérea (BA) y de raíces (BR). El método estadístico utilizado fue un diseño en bloques incompletos aleatorizados, se evaluaron dos tratamientos (T) (T1: tradicional y T2: silvopastoril) con cuatro bloques (B) en cada uno (B1: FCC, B2: FCX, B3: FR, B4: RP). El trabajo se realizó entre junio 2016 y junio 2017 en San Pedro de los Milagros, Antioquia Colombia. Los resultados permitieron determinar que las raíces a 20 cm de profundidad, el colchón muerto y las hojas, fueron los compartimentos con mayores existencias de carbono (4.52, 3.58 y 1.9 ton de C ha-1 respectivamente). Se encontraron diferencias (P < 0.05) entre relieve plano y el relieve rectilíneo para la biomasa de hojas, y entre el relieve plano con los demás relieves evaluados para la variable raíces gruesas a 20 cm de profundidad. La biomasa producida por la planta es directamente proporcional al carbono incorporado. La biomasa radicular, tanto para raíces finas como gruesas, contribuye a capturar en promedio 2 820 y 655 kg ha-1 de carbono a 20 y 40 cm de profundidad respectivamente. El pasto kikuyo contribuye a mantener reservas de carbono en las praderas. Por la alta producción de biomasa radicular, de colchón y la alta capacidad de rebrote en condiciones adversas, se concluye que este pasto juega un papel importante en la disminución de GEI y la conservación de los suelos del trópico alto bajo sistemas de lechería especializada.

The increase of Greenhouse Gases (GHG) emissions derived from human activities are considered the main cause of current climate change and the livestock sector is responsible for 18 % of the GHG emissions in CO2 equivalent. Kikuyu grass can optimize both carbon capture and carbon fixation. The aim of this paper was to identify carbon stocks in the kikuyu grass in its different compartments, above-ground biomass (AB) and below-ground biomass (BB) at 20 and 40 cm soil depth, under the traditional and silvopastoral systems in different reliefs. Six successive samplings (M) were taken according to the grazing system (traditional and silvopastoral system), and the geoform of the terrain (concave flank (CCF), convex flank (CXF), rectilinear flank (RF) and flat relief (FR)). The above-ground biomass and below-ground biomass were sampled. The statistical method used was a design in incomplete randomized blocks, two treatments were evaluated (T) (T1: traditional system and T2: silvopastoral system) with four blocks (B) in each one (B1: CCF, B2: CXF, B3: RF, B4: FR). This experiment was done from June 2016 to June 2017 in San Pedro de los Milagros, Antioquia, Colombia. The results allowed to determine that the roots at 20 cm depth, the dead creeping stems, and the leaves were the compartments with the highest carbon stocks (4.52, 3.58 and 1.9 ton of C ha-1, respectively). Differences were found (P < 0.05) between flat and rectilinear relief for the biomass of leaves, and between the flat relief with the other reliefs evaluated for the variable thick roots at 20 cm depth. The biomass produced by the plant is directly proportional to the incorporated carbon. The root biomass, both fine and thick roots, contributes to capture on average 2 820 kg and 655 kg of carbon per hectare at of 20 and 40 cm depth respectively. Kikuyu grass contributes to keep carbon reserves in the grasslands. Due to the high production of below-ground biomass and creeping stems, and its high capacity of regrowth under adverse conditions, this grass plays an important role in the reduction of GHG and the conservation of high tropical soils under specialized dairy systems.

Crepping attachment tardio ­ relato de caso com três anos de acompanhamento / Delayed creeping attachment occurence ­ a case report with 3 years of follow-up

O objetivo desse artigo foi apresentar, através de um caso clínico, o efeito do creeping attachment com acompanhamento constante até três anos após a cirurgia de recobrimento radicular. Paciente do sexo masculino relatava sensibilidade na região inferior do lado esquerdo e buscava resolução para sua queixa. Em exame físico, foram observadas recessões gengivais dos dentes 32 ao 35. Optou-se então pelo tratamento cirúrgico. Após incisão tipo sulcular e rebatimento do retalho de espessura parcial, realizou-se regularização das raízes dos dentes 33 ao 35. As papilas foram mantidas e em seguida foi realizado o enxerto de tecido conjuntivo heterógeno; o retalho foi posicionado coronariamente e suturado nas papilas remanescentes. Foi realizado o acompanhamento pós-cirúrgico nos períodos de sete, 30, 60 e 180 dias, dois e três anos. Com 180 dias já era possível notar a presença de tecido queratinizado bem definido e fixado na linha mucogengival. O fenômeno de creeping attachment foi observado dois anos e oito meses após a cirurgia. A partir do caso clínico, concluiu-se que, quando há pouca ou nenhuma perda de papila interproximal classificada em classe I e II de Miller, o creeping attachment certamente ocorrerá, desde que haja uma manutenção adequada da região.

The purpose of this article was to present, through a clinical case, the effect of creeping attachment with constant recalls up to three years after root coverage surgery. A male patient reported tenderness in the lower left mandibular side and sought resolution for his complaint. On physical examination, gingival recessions of the teeth 32 to 35 were observed. Then, the surgical treatment was proposed. After a sulcular type incision and partial-thickness flap elevation, the roots of the teeth 33 to 35 were regularized. The papillae were maintained and the heterogeneous connective tissue graft was performed. The flap was positioned coronally and sutured in the remaining papillae. Post-surgical follow-up was performed after 7, 30, 60 and 180 days, two and three years later. At 180 days it was already possible to notice the presence of a well-defi ned keratinized tissue attached to the mucogingival line. The creeping attachment phenomenon was observed after two years and eight months. From this clinical case, it was concluded that, when there is little or no loss of interproximal papilla (Miller's Class I and II), the creeping attachment will certainly occur, provided that there is adequate maintenance of the region.

Atypical Presentation of Cutaneous Larva Migrans: A Case Report & Literature Review

Cutaneous larva migrans, also known as “migrant linear epidermitis”, “beach worm”, “migranthelminthiasis”, “dermatitis serpiginosus” or “creeping eruption”. It is a zoonosis which caused byaccidental percutaneous inoculation of helminth larvae, usually parasites of the small intestines of catsand dogs. It typically presents as an itchy, erythematous, serpiginous, cutaneous eruption. We describea case of disseminated and extensive infection of cutaneous larva migrans in a 21-year-old traveler,leading a delay in diagnosis and treatment.

Creeping attachment nas cirurgias plásticas periodontais / Creeping attachment in periodontal plastic surgery ­ a literature review

O objetivo desta revisão da literatura foi compreender melhor o fenômeno descrito na literatura como creeping attachment. Uma busca eletrônica foi realizada na base de dados PubMed compreendendo um período de 40 anos. Foram selecionados 18 trabalhos (nove relatos de casos, um RCT, um RCT duplo-cego, seis ensaios clínicos, um caso-controle). As recessões variaram de estreitas e amplas, em sua maioria envolvendo as classes II e III de Miller. A área mais operada foi a de incisivos. O enxerto gengival livre foi a técnica mais utilizada. O sucesso no recobrimento variou entre 50% e 100%. O creeping attachment ainda é um tema pouco explorado, com pouco conhecimento sobre seu mecanismo fisiológico e sua previsibilidade. Ele pode ser detectado posteriormente ao primeiro mês da intervenção mucogengival, sendo, em geral, acima de 0,5-1 mm (em alguns casos entre 5 mm e 9 mm). Apenas dois trabalhos (um ensaio clínico e um RCT) não mostraram este fenômeno. Não há correlação entre o creeping attachment e o tipo de recessão gengival (estreita, larga, rasa e profunda). Como a metodologia usada é conflitante, mais trabalhos controlados serão necessários para desvendar a natureza deste fenômeno.

The aim of this literature review was to better understand the phenomenon described as creeping attachment. An electronic search was made at the PubMed database in the last 40 years. Eighteen papers were selected (9 case reports, 1 RCT, 1 doubleblind RCT, 6 clinical trials, and 1 case-control). Recessions varied from narrow to large, with most involving Miller's class II and III. The incisor region was the most accessed area. The free gingival graft was the most utilized technique. The success varied from 50% to 100%. The creeping attachment is still a theme to be further explored since little is known about its physiological mechanism and predictability. It can be detected in the fi rst month after the mucogengival intervention being up to 0.5-1.0 mm (in some cases, 5-9 mm). Only 2 studies (1 clinical trial, 1 RCT) did not verify this outcome. There is no correlation between the creeping attachment and the recession type (narrow, large, shallow, or deep). Since the methodology applied is not similar, more controlled studies are necessary to understand the nature of this phenomenon.

Diagnosis of Cutaneous Pili Migrans Assisted by Dermoscopy / 대한피부과학회지

No abstract available.

Diagnosis of Cutaneous Pili Migrans Assisted by Dermoscopy / 대한피부과학회지

No abstract available.

Rash in a foreign worker

Hookworm-related cutaneous larva migrans (HrCLM) is a zoonosis which is endemic in many subtropic and tropical countries including Malaysia. We report a case of a 40-year old plantation worker who presented with a pruritic rash on his abdomen. It is important for clinicians to diagnose and treat HrCLM promptly as this condition results in considerable morbidity when treatment is delayed.

Guided Tissue Regeneration Based Treatment of Root Coverage using Placental Membrane Allograft: A Case Report.

Gingival recession is defi ned as the apical migration of gingival margin to the cementoenamel junction. It results in root exposure which leads to clinical problems such as root surface hypersensitivity, root caries, cervical root abrasions, diffi cult plaque control, and diminished aesthetic/cosmetic appeal. Guided tissue regeneration based root coverage has emerged as a promising treatment modality. A variety of non-resorbable and bioresorbable membranes have been successfully used. Among resorbable membranes, collagen has been extensively studied. Recently, amnion membrane, the third generation membrane which is a placental derived tissue has been introduced. On the basis of the fi ndings of the present case report, it can be concluded that recently introduced amnion membrane (third generation membrane) are equally effi cacious in the treatment of gingival recession and use of this resorbable membranes with coronally advanced fl ap is a versatile treatment modality for root coverage of isolated buccal gingival recessions. Th e more gingival tissue thickness (gingival biotype) enhancement was observed with this membrane. Amnion membrane has certain additive advantages over the collagen membrane such as better handling properties and a thin diameter enabling it to mold according to the defect anatomy and root surfaces easily.

A Case Report of Cutaneous Larvae Migrans with Associated Fungal Superinfection in Uyo, Nigeria.

Aims: To describe a case of Cutaneous Larvae Migrans (CLM) with associated fungal and bacterial superinfection seen in the Tuberculosis and Infectious Disease Unit of University of Uyo Teaching Hospital. Presentation of Case: A 31-year old male reported to the Tuberculosis and Infectious Disease Unit with a history of creeping eruptions, itching and right foot swelling following gardening. Investigations revealed associated bacterial and fungal super infection. Symptoms resolved after treatment with Albendazole, antibiotics and antifungals. Discussion: Although CLM is self-limiting, non- recognition and home treatment using local herbal remedies may lead to associated superinfection, mask the presentation and lead to delayed diagnosis. Conclusion: CLM is not frequently reported in the tropics and primary health care workers are likely unfamiliar with its presentation, which could delay the treatment. Associated use of local herbal remedies lead to superinfection as seen in the index case may lead to missed and delayed diagnosis. This calls for increased awareness of the condition and its treatment for primary care workers.

A Case of Graves' Disease Following Subacute Thyroiditis Presented with Creeping / 대한갑상선학회지

The occurrence of Graves' disease following subacute thyroiditis (SAT) is rare. The pathophysiology of it is not well known. We report a case of Graves' disease following SAT presented with creeping. A 45-year-old woman presented with neck pain, and thyrotoxic symptoms. Neck pain migrated from left lobe to right lobe of the thyroid. Thyroid scan revealed decreased uptake in the both lobes except the superior portion of the right thyroid gland. Initially, the patient was diagnosed with SAT and treated with steroid therapy. Four months later, thyroid function test showed suppressed thyroid-stimulating hormone (TSH), elevated free thyroxine (T4) and TSH receptor antibody. Thyroid scan revealed increased uptake compatible with Graves' disease. The autoimmune alteration after SAT may lead to the development of Graves' disease in the susceptible patients. These patients should be monitored for the development of Graves' disease.

Larva migrans cutánea en un paciente viajero portador de dishidrosis: a propósito de un caso clínico / Cutaneous larva migrans in a traveling patient with dyshidrosis: a case report

Larva migrans cutánea es el resultado de una infección por nematodos y muestra una erupción serpiginosa característica. Con el aumento de los viajes a zonas tropicales, muchos pacientes pueden volver con ésta infección, que es a menudo, mal diagnosticada o tratada incorrectamente. Esta enfermedad cutánea parasitaria es causada por la migración de larvas provenientes de animales en la epidermis humana.

Cutaneous larva migrans is a result of a nematode infection and shows a characteristic creeping eruption. As travel to the tropics zone increases, many patients may be returning with this infection, which is often misdiagnosed or treated incorrectly. This parasitic skin disease is caused by the migration of animal hookworm larvae in the human epidermis.

A Case of Cutaneous Larva Migrans / 대한피부과학회지

Cutaneous larva migrans (CLM) is an uncommon cutaneous creeping eruption caused by the invasion and migration of nematode larvae into the skin. Burrowing larvae produce intensely pruritic, serpiginous, raised erythematous skin lesions. Most cases of CLM reported in Korea involved travelers returning from endemic areas with tropical or subtropical climates. We report herein a case of CLM with no history of travelling to endemic area and briefly review the relevant literature.

Identification of Microdochium bolleyi Associated with Basal Rot of Creeping Bent Grass in Korea

Symptoms of basal rot occurred sporadically on creeping bent grasses growing at a golf course in Hampyeong, Korea in April 2007. Ten isolates of Microdochium sp. were obtained from leaves and crowns of the diseased bent grasses. All isolates were identified as Microdochium bolleyi based on morphological, cultural, and molecular characteristics. This is the first report on M. bolleyi associated with basal rot on creeping bent grass in Korea.

A Case of Creeping Eruption / 대한피부과학회지

Creeping eruption is a syndrome characterized by the penetration in human skin of larvae from hookworms of various animals, mainly cats and dogs. A 26-year-old male presents with pruritic erythematous serpiginous linear skin lesion on the left lower leg for 2 weeks. The skin lesion was developed after walking in bare feet along the beach of an island in Philippines. Histopathologic finding showed a burrow containing numerous eosinophils and a few mononuclear cells in the epidermis. No parasite was found in multiple histologic section.

A Case of Creeping Eruption / 대한피부과학회지

Creeping eruption is a rare cutaneous serpiginous eruption caused by fhe accidental invasion of the skin by parasites. The patient was a 26 year-old female presented with a severe pruritic erythematous serpiginous linear skin lesion on the dorsal surface of left foot for 10 days. Histopathologic finding revealed a typical burrow containing inflammatory cells and necrotic epithelial cells in the epidermis and diffuse patch infiltration of mononuclear cells and eosinophils in the dermis. Laboratory examination shows eosinophilia. The patient was treated with oral flubendazole 500mg per day for 10 days. The skin lesion disappeared without. recurrence. We described a typical case of creeping eruption never reported in Korea.

A Case of Cutaneous Larva Migrans / 대한피부과학회지

A 58-year-old male presented with a pruritic, erythematous hread-like eruption on the right chest, which appeared as a small papule since 6 months prior to his visit. Histopathologic examination showed a burrow containing numerous eosinophiles and a few mononuclear cells in the epidermis. There was focal spongiosis unde the burrow. In the dermis, there was a diffuse and occasionally dense perivascular and periadnx 1 inflammatory cell infiltration consisting of eosinophiles and some lymphocytes. Multiple histologic sections were made without success in deizo strating parasitic tissue in the epidermis, however, the clinical and histopathologic features we onsistent with cutaneous larva migrans.

Superior Rectus Recession with Modified Hang-loose Technique in Rabbits

Hang-loose technique(HLT) is a reliable recession method in strabismus surgery. However, the loop length and the parallel of the anterior muscle border may not be sustained when the muscle was recessed behing the equator. This study was designed to assess the postoperative status of the large-recessed superior rectus muscle using the HLT and a modification of HLT(M-HLT) respectively in rabbits. Some of the recessed muscles with both techniques showed forward creeping. The average creeping was 0.5 mm on M-HLT and 1.1 mm on HLT in 7 mm recession group, and the difference was statistically significant(p=0.023). In 8 mm recession group, the average creeping was 1.0 mm on M-HLT and 1.8 mm on HLT. The difference was statistically significant(p=0.012). Although the parallel of the anterior muscle border was better on HLT than on M-HLT, the difference was not significant statistically. According to our result, M-HLT may be an useful alternative method in the cases of large recession with HLT.