Research progress in the application of hybrid cell membrane biomimetic nano-drug delivery system in cancer treatment / 国际生物医学工程杂志

The biomimetic strategy of using the cell membrane-coated nanoparticles can retain the physical and chemical properties of the nanoparticles and show the biological characteristics of the source cell membrane, which can further enhance the role of the nanodrug in tumor treatment. A hybrid cell membrane is the fusion of two or more different types of cell membranes. A hybrid cell membrane can endow nanoparticles with multiple biofunctions derived from the source cells compared with a single cell membrane. Hybrid cell membranes provide a foundation to stimulate extensive research into multifunctional biomimetic nano-drug delivery system (NDDS), which is expected to broaden the application of biomimetic nanotechnology in drug delivery systems. In this review paper, the types of hybrid cell membrane used to construct nano-drug delivery systems, the preparation and characterization methods, and cancer treatment research progress in recent years were reviewed.

Autologous hybrid cell fusion vaccine in a spontaneous intermediate model of breast carcinoma

Breast cancer is among the most common malignancies affecting women and reproductively intact female dogs, resulting in death from metastatic disease if not treated effectively. To better manage the disease progression, canine mammary tumor (CMT) cells derived from malignant canine mammary cancers were fused to autologous dendritic cells (DCs) to produce living hybrid-cell fusion vaccines for canine patients diagnosed with spontaneous mammary carcinoma. The high-speed sorting of rare autologous canine patient DCs from the peripheral blood provides the autologous component of fusion vaccines, and fusion to major histocompatibility complex-unmatched CMT cells were produced at high rates. The vaccinations were delivered to each patient following a surgical resection 3 times at 3-week intervals in combination with immuno-stimulatory oligonucleotides and Gemcitabine adjunct therapy. The immunized patient animals survived 3.3-times longer (median survival 611 days) than the control patients (median survival 184 days) and also appeared to exhibit an enhanced quality of life. A comparison of vaccinated patients diagnosed with inflammatory mammary carcinoma resulted in a very short median survival (42 days), suggesting no effect of vaccination. The data showed that the development of autologous living DC-based vaccine strategies in patient animals designed to improve the management of canine mammary carcinoma can be successful and may allow an identification of the antigens that can be translatable to promote effective immunity in canine and human patients.

Strumal Carcinoid of the Ovary: Report of a case

Strumal carcinoma of the ovary is rare tumor of germ cell origin characterized by intimate mixture of thyroid tissue and carcinoid. We report a strumal carcinoid tumor associated with ipsilateral follicular cyst and contralateral mature cystic teratoma of the ovary in a 47-year-old woman. Histologically it was composed largely of trabeculae partly of insular carcinoid and focally of individual thyroid follicles. Many cells in the carcinoid areas and even some follicular areas contained abundant argyrophile granules, and they also, in the corresponding areas, demonstrated thyroglobulin. Electron microscopic findings revealed abundant neurosecretory granules and colloid material in the same cell. We agree that this tumor is derived from hybrid showing thyroid and neuroendocrine differenciation.

HISTOCHEMICAL OBSERVATION OF FUSION CELL BETWEEN HUMAN CANCER CELL LINE OF ESOPHAGUS AND CHINESE HAMSTER OVARY CELL LINE / 解剖学报

The Eca-109 cell line (human cancer cell line of the esophagus) and CHO cell line (Chinese hamster ovary cell line) were used in the present experiment. Three groups of the fusion cells induced by PEG were obtained. These groups were: (1) the cells formed by the fusion of Eca-109 and Eca-109. (2) the fusion of CHO and CHO. (3) the hybrids of Eca-109 and CHO. The third group was demonstrated by autoradiography after incorporated H~3-thymine. The results indicate that the number of megalonucleated cells increases more and more, while the number of multinucleated cells gradually decreases in 10 days after the treatment by PEG in all groups.The forms of fusion cells of Eca-109 and Eca-109 or CHO and CHO similar to those of parent cells. Heterogeneous binucleated cells have the same form as one of parent ceils or as transitional form between two parent cells. The multinucleated cells have various shapes.Histochemical observations have shown that reactions of lactate dehydrogenase (LDH), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH) in the fused cells were stronger than mononucleated cells. The level of activity of nonspecific esterase (NSE) in heterokaryon was intermediate between two parent cells i.e. higher than CHO and lower than Eca-109.