RESUMO
Objective To study the correlation between the genetic diversity and limonin compounds content of Citri reticulatae Semen germplasm resources in Sichuan to provide a reference for screening the main source varieties of C. reticulatae Semen. Methods Firstly, NTSYSpc2.10e software was used to analyze the ISSR markers results of 35 C. reticulatae Semen samples from different habitats and different varieties in Sichuan. Then, UPLC was used to determine the contents of limonin compounds (limonin, nomilin, and obacunone). Finally, all the results were analyzed by SPSS 21.0 software. Results A total of 240 clear and detectable DNA bands were generated from 33 ISSR primers, and the variation range of genetic similarity coefficient were 0.72-0.92. Citrus reticulate ‘Ponkan’ and C. reticulate ‘Dahongpao’ have the closest relationship and their Limonin compounds contents were similar, but the relationship between other varieties and Citrus reticulate ‘Dahongpao’ was far and limonin compounds contents had great difference. Conclusion The relationship of variety is closer to the C. reticulata ‘Dahongpao’, the content of limonin compounds is higher, and Citrus reticulate ‘Ponkan’ could be considered as the main source variety of Citri reticulatae Semen.
RESUMO
Objective: To study the biosynthesis mechanism and transshipment law of limonin compounds in Citri Reticulatae Semen, traditional medicinal variety Citrus reticulata "Dahongpao". Methods: In the process of seed growth and development, the contents of limonin, nomilin, and obacunone in stem, vane, peel, and seeds were determined by UPLC tangerine; Using grey relational analysis method, the correlation analysis between the contents and the cloning of squalene synthase gene (ss), squalene epoxidase gene (se), and glucose transferase (lgt) expression was performed. Results: Limonin compounds in different organs had the largest accumulation in seeds; The accumulation of limonin compounds in different organs was significantly correlated with ss, se, and lgt gene expression, but in the seeds the accumulation of limonin has the most closely correlation with ss, se, and lgt gene expression; The ss gene expression had the largest contribution on the accumulation of limonin, nomilin, and obacunone. Conclusion: The study provides reliable and scientific envidendce for the synthesis mechanism and transshipment law of limonin compounds.