A modified mouse model of orthotopic left lung transplantation based on "pendulum" anastomosis of the reverse-view plane (with video demonstration) / 器官移植

Objective To modify the mouse model of orthotopic left lung transplantation from different perspectives, aiming to establish a simpler, faster and stabler mouse model of lung transplantation. Methods Based on preliminary modified rat model of orthotopic left lung transplantation established by our team, varying extent of modifications were made regarding the tracheal intubation, cannula preparation and anastomosis procedures of orthotopic left lung transplantation in the recipient mice. Orthotopic left lung transplantation in 40 mice were performed by an operator with microsurgical experience. The dissection of the recipient's hilar structure was carried out at the plane of the hilar clamp model within the reverse-view, and the three branches (left main bronchus, pulmonary artery and pulmonary vein) of the pulmonary hilum were anastomosed in turn by the "pendulum" anastomosis method. The operation time of each procedure was recorded. The recipient mice were sacrificed at postoperative 2 weeks, and the incidence of postoperative complications was recorded. Results Lung transplantation was successfully completed in 40 mice, with no bronchial and vascular tearing or twisting, and no bleeding at the anastomosis site. The overall cardiopulmonary procurement time was (10.7±1.5) min, cannula preparation time was (16.2±1.5) min, cold ischemia time was (25.1±2.4) min, warm ischemia time was (19.4±1.6) min, and the total operation time was (57.2±2.9) min, respectively. During the follow-up from 6 to 14 days after surgery, one recipient mouse died of pleural effusion, probably caused by infection. No pneumothorax, thrombosis or atelectasis was found in the remaining recipient mice during postoperative follow-up. Conclusions The modified mouse model of orthotopic left lung transplantation based on "pendulum" anastomosis of the reverse-view plane possesses multiple advantages of short operation time, high success rate and few complications, which is expected to become an alternative model of studying pathological changes after lung transplantation and worthy of further application.

Transcriptome analysis to identify genes involved in the biosynthesis of aconitines in Aconitum pendulum / 药学学报

italic>Aconitum pendulum is a Tibetan medicine that is rich in bioactive compounds such as aconitine-type C19-diterpenoid alkaloids. To investigate the key enzymes in the aconitine biosynthesis pathway, roots, leaves and flowers of Aconitum pendulum were subjected to a high-throughput transcriptomic sequencing analysis by Illumina HiSeqTM2000. Trinity de novo assembly yielded 47 264 unigenes with an average length of 1 140 bp and N50 of 1 678 bp, of which 30 231 unigenes (63.96%) were annotated. In the KEGG database, 542 unigenes were implicated in 17 secondary metabolic pathways; the analysis showed that 44 genes encoded 20 key enzymes in the diterpene skeleton of aconitine biosynthesis and 12 BAHD acyltransferase genes were related to the acetylation modification, with differential expression among three organs. For example, ApTPS8 was the only committed enzyme in the upstream aconitine biosynthetic pathway. The high expression level of ApTPS8 in root indicated that it is the main tissue for the production of precursors of diterpene alkaloids. Consistent with the accumulation of aconitine, we propose that ApBAHD1/2/8 is involved in the biosynthesis of 2-hydroxyaconitine, dehydrated 14-benzoylaconitine, 8-O-methyl-14-benzoylaconine, benzoyldeoxyaconitine and benzoylaconitine, and ApBAHD10 is involved in the biosynthesis of acontine, lucidusculine, 14-O-acetylneoline and 14-O-acetylvirescenin. Comparative transcriptome analysis of A. pendulum and A. carmichaeli indicates significant gene loss in the family of diterpene synthases and acyltransferases in A. pendulum, which is in accordance with the significantly fewer type and quantity of aconitine compounds in this species. Therefore, A. pendulum has proved to be an ideal material for the study of the aconitine biosynthesis pathway. This work provides basic scientific data for further study of aconitine biosynthesis, the discussion of molecular mechanisms of toxicity, and the synthesis of genuine medicinal materials.

Learning curve and key procedures analysis of modified rat models of orthotopic left lung transplantation / 器官移植

Objective To summarize and analyze the key procedures of the modified rat model of orthotopic left lung transplantation, aiming to provide more experience for the establishment of rat models of lung transplantation. Methods Two surgeons (A and B) performed complete transplantation in consecutive 15 rat models, and every 5 surgeries were divided into 1 practice stage. The operating time of each transplantation procedure was recorded. The differences of overall success rate and 1-week survival rate were calculated among different practice stages. The learning curve was delineated by the cumulative sum method. Results For surgeons A and B, the number of the first successful transplantation was the 5th and 6th time, the overall success rates of transplantation were 80% and 87% respectively, and the 1-week survival rates of rats both were 92%. Along with the increasing number of surgeries, the entire cardiopulmonary procurement, cannula preparation, cold ischemia, warm ischemia, transplantation and total operation time by two surgeons showed a significantly downward trend (all P < 0.05). For surgeons A and B, 3 and 2 rats died of heart failure due to overdose anesthesia, and 1 rat died of vein distortion at postoperative 1 d and 1 died of atelectasis at postoperative 7 d, respectively. The goodness of fit (R2) of cumulative sum method was 0.992 8 and 0.976 6. The turning point of learning curve was achieved in the 7th and 8th transplantation for surgeons A and B. Conclusions The modified rat model of orthotopic left lung transplantation yields high operability and repeatability both theoretically and technologically, and has multiple advantages of short learning curve, short operation time, high survival rate of the recipients and few complications, which is worthy of application in the basic research of lung transplantation.

Study on multi-index components of Tibetan medicine Bangna of Aconitum genus according to fingerprints and chemometrics / 中草药

Objective: To establish the chemical fingerprint and multi-index components determination of Tibetan medicine Bangna of Aconitum genus, and provide references for the formulation of quality standards of multi-base original medicinal materials and clinically safe medication. Methods: HPLC fingerprint of Bangna was established and evaluated by the similarity evaluation system of TCM. In addition, the content of the seven components of Bangna from 30 batches and the difference of chemical information between the two species of Bangna was investigated by principal component analysis and orthogonal partial least squares discrimination analysis (OPLS-DA) respectively. Results: A total of 17 common peaks were identified in the fingerprint that was established by the determination of 30 batches of Bangna, and seven components of which were identified with 12-epi-napelline, songorine, benzoylmesaconitine, benzoylaconine, mesaconitine, aconitine, 3-acetylaconitine. Based on similarity results, the fingerprint had good consistency between the same origin and minor diversity between the different sources. The results of principal component analysis and OPLS-DA showed that there were some differences in the content of seven components between the two species. Based on the results of OPLS-DA and t test, it could be determined that the contents of 12-epi-napelline and aconitine of Aconitum flavum were significantly higher than those in Aconitum pendulum (P < 0.01). Conclusion: The fingerprint and multi-component quantitative analysis methods were used for the quality and clinically safe medication control of Bangna in this paper is simple, easy to operate, and informative. Moreover, it is necessary to establish and improve the limit determination of diester alkaloids.

Clinical effect of bone-anchored pendulum in treatment for class Ⅱ malocclusion / 中华医学美学美容杂志

Objective To study the clinical effect of bone-anchored pendulum in treatment for class Ⅱ malocclusion .Methods Twelve patients treated by bone-anchored pendulum were chosen .In each patient ,two microscrews were inserted in the anterior paramedian region of the median palatal suture ,6-9 mm posterior to the incisive foramen and 3 – 6 mm lateral to the median line .Pendulum that based on the microscrews was used to distalize maxillary first molar ,and the force applied was 250 g .Skeletal and dental changes were measured on cephalograms before (T1 ) and after (T2 ) distal-ization .Results Class Ⅰ molar relationship had been obtained 7 month after distalization .The aver-age distal movement of the maxillary first molars was 7 .07 mm ,and the inclination was 9 .93° .The second premolars were distalized an average of 5 .44 mm with inclinations of 5 .22° .The first premolars were distalized an average of 4 .09 mm with inclinations of 3 .95° .The maxillary anterior teeth were re-truded 0 .93 mm and palatally inclined 1 .66° .There was statistical difference in that change .Conclusions By the use of microscrew ,pendulum can distalize maxillary molar effectively without mesial move-ment of premolar and labial movement of incisor ,and no loss of dental anchorage is observed during the distal movement .

Repercussão da anquiloglossia em neonatos: diagnóstico, classificação, consequências clÍnicas e tratamento / Repercussion of ankyloglossia in neonates: diagnosis, classification, clinical consequences and treatment

Introdução: A anquiloglossia é uma anomalia congênita que envolve alterações morfofuncionais no frênulo lingual e que pode estar atrelada às condições genéticas; apresentando-se em maior frequência no sexo masculino; e caracteriza-se por limitar os movimentos linguais. Com isso, pode contribuir na disfunção do sistema estomatognático, desencadear prejuízos na amamentação e comprometer o desenvolvimento craniofacial do neonato. Objetivo: As divergentes opiniões encontradas promovem um quadro de incerteza na escolha do protocolo de avaliação ou na intervenção, impulsionando assim a realização deste artigo. O presente artigo objetivou revisar a literatura de modo a desenvolver o conhecimento nos protocolos de avaliação para o diagnóstico da anquiloglossia, analogamente verificar as interferências condicionadas por esta anomalia no sistema estomatognático e observar as formas de tratamento para liberação do frênulo lingual. Materiais e Métodos: Foi realizada uma revisão de literatura de estudos anteriores com acesso possibilitado pelo PubMED/Medline, Lilacs, Scielo, Scopus; estabelecido o critério de artigos recém-publicados, no intervalo de 2015-2019. Conclusão: Assim, a ausência de um método de identificação que se enquadre em um padrão ouro revela a necessidade de realização de mais estudos sobre um protocolo mais eficaz para diagnóstico e classificação dessa anomalia, pois um método com maior nível de exatidão evitará intervenções inoportunas, e promoverá o conhecimento básico para realização do tratamento adequado para cada quadro de anquiloglossia.

Introduction: Ankyloglossia is a congenital anomaly involving morphofunctional changes in the lingual frenulum and which may be linked to genetic conditions; presenting more frequently in males; it is characterized by limiting lingual movements, which can contribute to the dysfunction of the stomatognathic system, triggering losses in breastfeeding and compromising the craniofacial development of the newborn. Objective: The divergent opinions found promote a picture of uncertainty in the choice of the evaluation protocol or intervention, thus driving the realization of this article. This article aimed to review the literature in order to develop knowledge in the assessment protocols for the diagnosis of ankyloglossia, similarly to verify the interferences conditioned by this anomaly in the stomatognathic system and to observe the forms of treatment for release of the lingual frenulum. Materials and Methods: A literature review of previous studies with access made possible by PubMED / Medline, Lilacs, Scielo, Scopus; the criterion for newly published articles in the 2015-2019 range was established. Conclusion: Thus, the absence of a gold-standard identification method reveals the need for further studies on a more effective protocol for diagnosing and classifying this anomaly, as a more accurate method will avoid inappropriate interventions, and will promote the basic knowledge for carrying out the appropriate treatment for each ankyloglossia picture.

Analysis of effects from usage of skeletal anchorage-assisted Pendulum appliance on vertical component of craniofacial structure

PURPOSE: The purpose of this study was to evaluate distalizing effects from the Pendulum appliance on vertical component of craniofacial structures. MATERIALS AND METHODS: 20 Patients who visited for orthodontic treatments are assigned to two groups. Group I, SN-MP > 37 degrees are showing hyperdivergent pattern. Group II, 29 < SN-MP < 37 degrees are showing mesocephalic pattern. Each group are consisted of 10 people. RESULTS AND CONCLUSIONS: Differences between skeletal classifications result in significant differences at labioversion of lower incisors and distalized amount, which is larger at Group I (P <.05). Group II has only shown significant distalized molars (P < .05). Labioversion of lower incisors has not shown significant change. Skeletal anchorage-assisted Pendulum appliance doesn't deteriorate vertical component nor significantly improve.

Content Determination of Three Alkaloids in Aconitum Pendulum and Habitually Medicinal Materials / 中国中医药信息杂志

Objective To establish a method for determining benzoylaconine, aconitine and 3-deoxyaconitine in Aconitum pendulum and the habitually medicinal materials by HPLC; To provide basis for control of quality standard toxicity composition in Aconitum pendulum and difference between Aconitum pendulum and the habitually medicinal materials. Methods Agilent Eclipse XDB-C18 column (250 mm × 4.6 mm,5 μm) was used at 25 ℃ with the mobile phase consisting of acetonitrile-0.04% trimethylamine (each 1000 mL of water plus 4 mL triethylamine and 1.68 mL phosphoric acid) by gradient elution; detection wavelength was 235 nm; the flow rate was 1 mL/min; injection volume was 10 μL. Results The benzoylaconine, aconitine and 3-deoxyaconitine had good separation and linear relationship in the corresponding range (r＞0.999). The average recovery rates were 97.66%–98.47%, and RSD were 0.84%–1.60%. Conclusion The contents of 3 alkaloid were different in Aconitum pendulum and the habitually medicinal materials. Both A. polyschistum and A. sessiliflorum need separate drug names.

Hernioplastia incisional y abdominoplastia simultáneas como métodos quirúrgicos seguros y eficaces / Simultaneous incisional hernioplasty and abdominoplasty as effective and safe surgical methods

Introducción: la hernioplastia-abdominoplastia simultáneas producen resultados clínicos-estéticos favorables en pacientes con exceso de piel y tejido graso en abdomen inferior. Objetivo: evaluar los resultados de estos métodos quirúrgicos. Método: se efectuó un estudio prospectivo de 16 pacientes operadas de hernia incisional y abdomen péndulo en el Hospital General Universitario Dr Juan Bruno Zayas Alfonso de Santiago de Cuba, durante el bienio 2015-2016. Resultados: predominaron la hernia infraumbilical, el anillo de 10-15 centímetros, las técnicas de dermolipectomía y Rives, así como el seroma como complicación posoperatoria. La edad media fue de 40 años; se realizó profilaxis antimicrobiana y antitrombótica. Se usó la prótesis de polipropileno en 100,0 por ciento de las afectadas. Conclusión: la eventroplastia y abdominoplastia son métodos seguros y eficaces en pacientes adecuadamente seleccionados

Introduction: simultaneous hernioplasty-abdominoplasty provoque favorable clinic-cosmetic results in patients with excess skin and adipose tissue in the lower abdomen. Objective: to evaluate the results of these surgical methods. Method: a prospective study of 16 operated patients with incisional hernia and pendulum abdomen was carried out in Dr Juan Bruno Zayas Alfonso University General Hospital in Santiago de Cuba, during 2015-2016. Results: There was a prevalence of the imphraumbilical hernia, 10-15 cm ring, dermolipectomy and Rives technique, as well as seroma as postoperative complication. The mean age was 40 years; antimicrobian and antithrombotic prophylaxis were carried out. The polypropylene prosthesis was used in 100 percent of the affected patients. Conclusion: eventroplasty and abdominoplasty are safe and effective methods in patients adequately selected

Postharvest quality and functional compounds in "dedo-de-moça" 'BRS Mari' pepper fruit at different stages of maturity / Qualidade pós-colheita e compostos funcionais de pimenta dedo-de-moça 'BRS Mari' em diferentes estádios de maturação

ABSTRACT: The purpose of this study was to evaluate the physicochemical characteristics and functional properties of "dedo-de-moça" 'BRS Mari' pepper fruits at different maturity stages, and determine the ideal harvest stage for fresh consumption. The pepper plants were grown in the experimental field of Embrapa Hortaliças (Brasília, DF, Brazil) in 2012, and their fruits were evaluated at 20, 30, 40, 50, 60, 70, and 80 days after anthesis (DAA) to determine the soluble solid content (SS), titratable acidity (TA), SS/TA ratio, color (hue angle and lightness), a, b, and total chlorophyll, total phenolic compounds (TPC), total antioxidant activity (TAA), and capsanthin. It was observed that SS content increased until 70 DAA, and TA increased until 50 DAA, with decrease at 80 DAA. The a and total chlorophyll decreased until 60 DAA. Values for chlorophyll b were high until 30 DAA, and then decreased. The values for hue angle and lightness decreased until 60 DAA, indicating a change from green to red in fruits external color, with small changes in color from 60 to 80 DAA. The TPC content increased until 60 DAA, and then small increases occurred until 80 DAA. The AA increased as the fruit ripened, and the capsanthin content increased until 70 DAA. These results indicated that the ideal stage for harvest of the "dedo-de-moça" 'BRS Mari' pepper fruits is at 70 days after anthesis, when they also have a full development of red color in the fruit epidermis.

RESUMO: O objetivo deste trabalho foi avaliar as características físico-químicas e propriedades funcionais da pimenta dedo-de-moça 'BRS Mari', em diferentes estádios de maturação, e determinar o estádio ideal de colheita dos frutos destinados ao consumo in natura. As pimentas foram cultivadas no campo experimental da Embrapa Hortaliças (Brasília, DF, Brasil), em 2012. Seus frutos avaliados aos 20, 30, 40, 50, 60, 70 e 80 dias após a antese (DAA) quanto ao teor de sólidos solúveis (SS), acidez titulável (AT), relação SS/AT, coloração (ângulo hue e luminosidade), clorofilas a, b e total, compostos fenólicos totais (CFT), atividade antioxidante (AAT) e capsantina. Observou-se que o teor de SS aumentou até 70 DAA e a AT aumentou até os 50 DAA com decréscimo aos 80 DAA. Clorofilas a e total diminuíram até 60 DAA. Os valores de clorofila b foram mais elevados até 30 DAA e depois diminuíram. Os valores de ângulo hue e luminosidade diminuíram até 60 DAA, indicando mudança de verde para vermelho na 'coloração externa' dos frutos, com pequenas mudanças na coloração de 60 até 80 DAA. O conteúdo CFT aumentou até 60 DAA e depois pequenos aumentos ocorreram até 80 DAA. AAT aumentou à medida que os frutos amadureciam e o conteúdo de capsantina aumentou até 70 DAA. Esses resultados indicam que o estádio ideal para colheita da pimenta dedo-de-moça 'BRS Mari' é aos 70 dias após a antese, quando ela também apresenta o total desenvolvimento da coloração vermelha na epiderme.

Contrastive Study of Ranunculaceae Aconitum Pendulum Busch and Aconitum Kongboense Lauener / 中国药师

Objective: To identify Aconitum pendulum Busch. and Aconitum kongboense Lauener. Methods: Aconitum pendulum Busch. and Aconitum kongboense Lauener were identified by microscopical identification and TLC. And the contents of aconitine in the two herbs were determined by HPLC. Results:Microscopical identification showed they were different from each other, and aconitine was used as the reference component. Using the solution consisting of hexane ∶ethyl acetate ∶diethylamine (10 ∶6 ∶0. 8) as the developing solvent, the compositions in the two herbs were different in TLC. The content of aconitine in Aconitum pendulum Busch was 0. 71 mg· g-1 , while that in Aconitum kongboense Lauener was only 0. 03 mg·g-1 . Conclusion:Aconitum pendulum Busch. and Aconitum kong-boense Lauener Identified by microscopical identification, TLC and HPLC,SHOW NOTABLE differences between them, and Aconitum kongboense Lauener should not be used as Aconitum pendulum Busch.

Induction and proliferation culture of Adventitious roots of Aconitum pendulum Busch / 中国药学杂志

OBJECTIVE: To establish the induction and culture system of adventitious roots of Aconitum pendulum Busch. METHODS: By tissue culture, the influences of different explants and plant growth substances on the induction and proliferation of adventitious roots of Aconitum pendulum Busch were investigated. RESULTS: Mixture of MS+2.5 mg·L-1 indole-3-butyric acid (IBA)+0.5 mg·L-1 6-benzylaminopurine (6-BA)+1.0 g·L-1 polyvinyl pyrrolidone (PVP) was the best callus induction medium. Mixture of MS+0.5 mg·L-1 IBA+1.0 g·L-1 PVP was the optimum adventitious root induction medium. Mixture of MS+0.5 mg·L-1 IBA+1.0 g·L-1 PVP was the most suitable adventitious root proliferation medium. CONCLUSION: A culture system is preliminarily established for the induction and proliferation of adventitious roots of Aconitum pendulum, which provides a new way for the resource exploitation and utilization of Aconitum pendulum Busch.

Preliminary Study on Adventitious Root Induction and Culturing ofAconitum Pendulum Busch / 中国中医药信息杂志

Objective To establish the adventitious root induction and culture system ofAconitum pendulum Busch.Methods The influence of different explants and plant growth substances on adventitious induction proliferation ofAconitum pendulumBusch were investigated through tissue culturing.Results Explants induction ability was: root>stem>leaf. 2.5 mg/L IBA was in favor of induction and multiplication adventitious root ofAconitum pendulumBusch.ConclusionThe culture system of induction and proliferation adventitious roots ofAconitum pendulum Busch was preliminary established, which provides a new way for resource exploitation and utilization of Aconitum pendulum Busch.

Changes produced by the pendulum appliance with skeletal anchorage and comparison with conventional pendulum appliance / Alterações produzidas com o aparelho pêndulo com ancoragem esquelética e comparação com o aparelho pêndulo convencional

Os propósitos destas pesquisas foram primeiramente avaliar as alterações esqueléticas, dento-alveolares e tegumentares obtidas com o aparelho pêndulo com ancoragem esquelética (BAPA) em pacientes com má oclusão Classe II e, posteriormente compará-los com os efeitos produzidos com o aparelho pêndulo com ancoragem convencional (CPA). A amostra composta de 18 pacientes (14 meninas;4 meninos) com idade média de 14.01 (D.P. 1.08) com má oclusão de Classe II tratados com BAPA como dispositivo distalizador antes do aparelho fixo, no Departamento de Ortodontia da Faculdade de Odontologia da Universidade Cidade de São Paulo. Telerradiografias laterais foram tomadas no início do tratamento (T0) e no final da distalização (T1), mensuradas e posteriormente analisadas estatisticamente. Apenas o período da distalização ativa foi avaliado. O grupo controle foi composto de 18 pacientes (14 meninas;4 meninos) com idade média de 13.61 (D.P. 1.24) com a mesma má oclusão tratados com CPA como dispositivo distalizador antes do aparelho fixo, no Departamento de Ortodontia da Faculdade de Odontologia de Bauru, Universidade de São Paulo. Para avaliar os efeitos desta amostra foi utilizada a mesma metodologia acima mencionada. Posteriormente, para comparar as alterações entre os grupos, a amostra tratada com CPA foi denominada Grupo 1 (G1), e a amostra tratada com BAPA denominada Grupo 2 (G2). O tempo médio de distalização foi 5.76 e 6.16 meses, no G1 e G2 respectivamente. Ambos os grupos foram compatibilizados em relação à idade inicial, tempo de tratamento, severidade da má oclusão de Classe II e características cefalométricas iniciais. A comparação intra-grupo foi realizada com teste t dependente, e a comparação intergrupos foi realizada com o teste t. Em G2 houve aumento significante da altura facial anteroinferior, distalização molar, inclinação distal e intrusão significante dos primeiros e segundos molares superiores. Os primeiros pré-molares apresentaram distalização e extrusão significantes. A sobremordida diminuiu e a relação molar melhoraram significativamente. Comparando as alterações do tratamento entre grupos, foram significativamente maiores a distalização, inclinação distal e intrusão dos primeiros molares no G2 comparados ao G1. Os incisivos superiores inclinaram para vestibular no G1, e ligeiramente retroinclinaram para lingual no G2, além disso os primeiros pré-molares foram distalizados no G2, porém foram mesializados no G1. O Grupo 1 apresentou aumento significativamente maior da sobressaliência, e menor correção da relação molar de Classe II do que G2. A distalização dos molares superiores foi obtida com sucesso em ambos os grupos, entretanto, utilizando o BAPA observou-se várias vantagens, tais como: Distalização exclusiva dos molares superiores; movimento distal espontâneo dos pré-molares superiores; sem efeitos colaterais indesejáveis nos incisivos superiores e nos lábios; e também o estabelecimento de uma relação molar de Classe I.(AU)

The purposes of these investigations were firstly to evaluate the dentoalveolar, skeletal and soft tissue effects obtained with the bone-anchored pendulum appliance (BAPA) in patients with Class II malocclusion and secondly, to compare these effects with the conventional pendulum appliance (CPA). A sample of 18 patients (14 females; 4 males) with a mean age of 14.01 years (S.D 1.08) with Class II malocclusion treated with BAPA as distalizer device before fixed appliance in Orthodontic Department at the Dental School, University of São Paulo City. Lateral headfilms were taken at the beginning of treatment (T0) and at the end of distalization period (T1), measured and then statistically analyzed. Only the active distalization period was evaluated. The comparison group was composed by 18 patients (14 females; 4 males) with a mean age of 13.61 years (S.D 1.24) with the same malocclusion treated with CPA before fixed appliance in Orthodontic Department at Bauru Dental School, University of São Paulo. To evaluate the effects in this sample was used the same methodology abovementioned. Then, to compare changes between groups, the sample treated with CPA was named Group 1 (G1), and the sample treated with BAPA was named Group 2 (G2). The average distalization period was 5.76 and 6.16 months, respectively for G1 and G2. Both groups were compatibilized regarding initial age, treatment time, severity of Class II malocclusion and initial cephalometric characteristics. Intragroup comparisons were performed using dependent t tests and intergroup comparisons were performed using t tests. In G2 there was significant increase in the lower anterior face height, molar distalization, distal tipping and intrusion of the first and second maxillary molars. The first premolars had significant distalization and extrusion. Overbite significantly decreased and molar relationship was significantly improved. Comparing the groups, there were significantly greater maxillary first molar distalization, distal tipping and intrusion in G2 compared to G1. The maxillary incisors were labially tipped in G1 and lingually tipped in G2. The first premolars were distalized in G2 while were mesialized in G1. G1 showed a significantly greater overjet increase and smaller Class II molar relationship correction than G2. Distalization of the maxillary molars was successfully achieved in both groups, but using the BAPA several advantages were observed including exclusive maxillary molars distalization, spontaneous distal movement of the maxillary premolars, no undesirable side effects in the maxillary incisors and lips and establishment of a Class I molar relationship.(AU)

Modification of pendulum appliance / 实用口腔医学杂志

Among several intraoral distalizing devices,the pendulum appliance is one of the most commonly used noncompliance appli-ances.It is widely used to correct Class Ⅱ molar relationship.More healthy teeth can be preserved with the using of pendulum appliance in the tooth extraction of borderline cases.The pendulum appliance was modified and used in clinic.Long-term observasion showed that the modified pendulum appliace is effective in orthodontic treatment.

Dentoalveolar and skeletal changes consequent to maxillary molar distalization with the modified pendulum appliance in classII malocclusion / 安徽医科大学学报

21 classII malocclusion adolescents (9 boys and 12 girls) were selected as the subjects, aged from 10.6 to 14.5 years, and the initial mean age was 12.8 years. All were treated with the modified Pendulum appli-ance. The duration for distalization of maxillary molars was from 3.2 to 5.7 months (4.3 months on average). Lat-eral cephalograms were obtained before and after distalization. Changes produced by the modified Pendulum appli-ance were analyzed with paired t tests. The mean space opening on lateral cephalograms was 7.31 mm, and maxil-lary first molar distalization accounted for 64.8% of the space,with a mean distal crown tipping of 18.64o. The rate of molar movement was 1.27 mm per month. The maxillary first molars intruded 0.69 mm,and the premolars extru-ded 1.02 mm. Lower anterior facial height increased 2.19 mm. The maxillary incisors had increased 3.39oof labial tipping and 1.13 mm of protrusion.

Comparación clínica y radiográfica del efecto del péndulo con anclajeesquelético vs. dentoalveolar / Clinical and radiographic comparison of the pendulum effect with skeletal

Introducción: este estudio pretende comparar clínica y radiográficamente los efectos producidos por el distalizador péndulo en dos formas de anclaje: esquelético y dentoalveolar, en pacientes con maloclusiones clase II de la Facultad de Odontología de la Universidad de Antioquia. Métodos: se hizo un estudio preexperimental comparativo. Se utilizó una muestra de 19 pacientes con edades entre los 15y 26 años, divididos en dos grupos: 9 pacientes tratados con péndulo de anclaje esquelético y 10 con péndulo de anclaje dentoalveolar. A todos los pacientes se les tomaron radiografías cefálicas laterales y modelos de estudio, al inicio y al final del tiempo de evaluación (6 meses). Resultados: los resultados clínicos y radiográficos muestran diferencias significativas en los efectos producidos a nivel de incisivos y molares. El péndulo con anclaje dentoalveolar presentó en promedio proinclinación de los incisivos de 4°, mientras que el péndulo conanclaje esquelético no mostró variación. En cuanto al efecto producido en los molares se observó mayor cantidad de distalación por inclinación (14,1°) en el péndulo con anclaje dentoalveolar que en el péndulo con anclaje esquelético (7,34°). Conclusiones: el péndulobajo las dos modalidades de anclaje mostró ser un dispositivo eficaz para distalar molares, sin embargo es importante tener en cuenta el efecto que se quiere producir sobre los incisivos, pues cada uno produce resultados diferentes.

Introduction: This study seeks to clinically and radiographically compare the effects produced by the distalization pendulum in both skeletal and dentoalveolar anchorage, in patients with Class II malocclusions at the Universidad de Antioquia’s School of Dentistry. Methods: a pre-experimental comparative study was carried out on a sample of 19 patients aged 15-26 years, divided into two groups:9 patients treated with bone anchorage pendulum and 10 with dentoalveolar anchorage pendulum. Lateral cephalic radiographs of each patient were taken and study models were done at the beginning and the end of evaluation period (6 months). Results: both clinical and radiographic results show significant differences in the effects produced at the incisors and molars. The pendulum with dentoalveolar anchorage presented an average incisor proclination of 4°, while the pendulum with skeletal anchorage showed no variation. Concerning the effect produced in molars, a greater amount of distalization as a result of inclination was observed in the pendulum with dentoalveolar anchorage (14.1°) in comparison to the one with skeletal anchorage (7.34°). Conclusions: with both modalities, the pendulum provedto be effective for molar distalization; nevertheless, it is important to bear in mind the desired effect on the incisors, as each technique produces different results.

Clinical effects of pendulum and straight wire technology in patients with fully erupted maxillary second molar / 中华医学美学美容杂志

ObjectiveTo investigate the clinical effect of pendulum and straight wire technology in patients with fully erupted second molar.MethodsPatients with Angle class Ⅱ malocclusion were chosen.Control group included patients without erupted second molar.Experimental group included patients with completely erupted second molar.Pendulums were used in two groups to distalize maxillary molars.Then straight wire orthodontic treatment had been performed until treatment was over.Cephalograms of each stage were analyzed.ResultsIn the experimental group,mesiobuccal cusp of first molar was distalized for 4.62 mm,geometric center was distalized for 3.75 mm,about 81 percent of the movement of mesiobuccal cusp.In control group,mesiobuccal cusp of first molar was dis talized for 5.78 mm,geometric center was distalized for 3.20 mm,about 55 percent of the movement of mesiobuccal cusp.It turned out first molar was distalized in both groups,but more rotation took place in control group than that in the experimental group.ConclusionsPendulum can distalize maxillary first and second molars in patients with completely erupted second molar.The pendulum and straight wire technology can treat these patients successfully.

Germinação de pimentas cambuci submetidas à superação de dormência em água quente / Germination of pepper seeds subjected to dormancy breaking in hot water

As sementes de pimenta cambuci apresentam dormência provocada talvez por impermeabilidade ou por inibidores presentes no tegumento. Esse trabalho teve como objetivo avaliar o percentual de germinação das sementes da pimenta variedade Cambuci Capsicum baccatum L. var. pendulum. O trabalho foi realizado no Laboratório de Analises de Sementes no Departamento de Fitotécnia no Centro de Ciências Agrárias da Universidade Federal da Paraíba. As sementes extraídas destas pimentas foram submetidas à quebra de dormência em água quente a 50, 60 e 70ºC por 10 minutos em seguida semeadas em sacos plásticos. Foram utilizadas 60 sementes por tratamento com 12 repetições de 5 sementes. Foram avaliadas a percentagem de germinação e o índice de velocidade de germinação para a testemunha e os tratamentos de imersão em água quente nas três temperaturas. Na testemunha ocorreu um número menor de sementes com dormência comparando com os tratamentos imersos em água quente, mais foi visto justamente ao contrario, ou seja, dois dos tratamentos imersos em água quente (50 e 60º C) proporcionaram uma maior germinação (ou seja, menor numero de sementes dormentes) comparada a testemunha.

Cambuci pepper seeds may have dormancy caused by impermeable or inhibitors present in the seed coat. This study aimed to evaluate the percentage of germination of pepper variety Cambuci Capsicum baccatum var pendulum. The work was conducted at the Laboratory of Seed Analysis in the Department of Crop Science in Agricultural Science Center, Federal University of Paraíba. The seeds from these peppers were subjected to break dormancy in hot water at 50, 60 and 70 º C for 10 minutes and then planted in plastic bags. 60 seeds were used per treatment with 12 replicates of 5 seeds. We evaluated the germination rate and speed of germination for the control and treatment of immersion in hot water at all temperatures. In witness there was a smaller number of seed dormancy compared with the treatments immersed in hot water, it has been just the opposite, ie two treatments immersed in hot water (50 and 60 degrees C) led to greater germination (ie smaller number of dormant seeds) compared to control.

Maloclusión Clase III: corrección con distalización superior e inferior / Class III malocclusion: correction with superior and inferior distalization

El tratamiento de las maloclusiones clase III incluye diferentes alternativas desde las terapias ortopédicas en edades tempranas, compensaciones dentales con ortodoncia o tratamiento ortodóncico-quirúrgico. La distalización de molares ha sido ampliamente utilizada en el arco superior para la corrección de maloclusiones clase II y el apiñamiento dental superior, y un poco menos en el arco inferior para ayudar a la corrección de leves apiñamientos y las clases III. Este reporte de caso muestra la corrección de la maloclusion clase III con el uso de la tracción cervical mandibular desde edad temprana para lograr un efecto ortopédico y distalizar molares inferiores evitando extracciones dentales, y el uso de un péndulo óseo soportado para distalizar molares superiores y corregir la discrepancia dentoalveolar. La terapia de combinación ortopédica-ortodóncica tuvo una duración de 40 meses y el uso de la distalización dental bimaxilar probó ser un método efectivo para la corrección de la clase III esquelético dental sin extracciones, con un buen resultado en la estética facial.

The treatment of Class III malocclusion includes different alternatives ranging from orthopedic therapy at early ages, orthodontic dental compensations or orthodontic-surgical treatment. Molar distalization has been widely used in the upper arch for the correction of Class II malocclusion and dental crowding; it has not been used as much in the lower arch where it is used to correct moderate crowding and Class III malocclusion. This case report shows the correction of a Class III malocclusion by means of the use of mandibular cervical headgear from an early age in order to obtain an orthopedic effect and distalization of lower molars, avoiding teeth extractions; a bone supported pendulum was usedin the upper arch to distalize molar and to correct the dentoalveolar discrepancy. The orthodontic orthopedic combined therapy lasted 40 months and the bi-maxillary dental distalization proved to be an effective method for the correction of skeletal dental Class III malocclusion, obtaining a good esthetic result.