Analysis of the clinical effects of the combination of mycophenolate mofetil with either tacrolimus or cyclophosphamide

OBJECTIVES: Here, we aimed to compare the clinical effects of mycophenolate mofetil combined with either tacrolimus or with cyclophosphamide on lupus nephritis (LN) and to analyze their influence on the expression of cystatin C and on transforming growth factor-1 (TGF-β1). METHODS: A total of 234 patients were randomly divided into two groups: group A, for mycophenolate mofetil combined with tacrolimus (n=117) and group B, for mycophenolate mofetil combined with cyclophosphamide (n=117). The enzyme-linked immunosorbent assay was adopted to detect the expression levels of serum TGF-β1 and cystatin C before and after treatment. RESULTS: The total effectiveness rate in group A was much higher than that in group B. The times of effectiveness and effect validity in group A were much lower than those in group B. The expression levels of serum TGF-β1 and cystatin C decreased slightly after treatment in the two groups, and those of group A were much lower than those of group B. CONCLUSIONS: The combination of mycophenolate mofetil and tacrolimus showed better clinical efficacy on LN and was safer than that of mycophenolate mofetil and cyclophosphamide. Moreover, the drug combination of mycophenolate mofetil and tacrolimus greatly reduced the expression levels of serum TGF-β1 and cystatin C.

Effects of brd4 gene on apoptosis and p38mapk signaling pathway of cardiomyocytes induced by tgf-pi / 吉林大学学报(医学版)

Objective: To investigate the effect of bromodomain-containing protein 4 (BRD4) gene on the apoptosis of cardiomy

Expression of transforming growth factor 1 and bone morphogenetic protein 9 in human nonunion tissues and its clinical significance / 中南大学学报(医学版)

Objective:To examine the expression of transforming growth factor I(TGF-β1) and bone morphogenetic protein-9 (BMP-9) in human nonunion tissues,and to evaluate the clinical significance.Methods:The number of hypertrophic nonunion tissue samples and atrophic nonunion tissue samples were collected from Department of Orthopedics,the Second Xiangya Hospital of Central South University and Suzhou Kowloon Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University between 2010 and 2014.Semi-quantification of SP immunohistochemical method and pathological image analysis software IPP6.0 were used to analyze the expression of TGF-β1 and BMP-9.Nonunion type,patients' age and nonunion time were statistical analyzed.Results:The absorbance values of TGF-β1 and BMP-9 in the hypertrophic nonunion tissues were 0.3236±0.0390 and 0.1337±0.0400,respectively;while the absorbance values of TGF-β1 and BMP-9 in the atrophic nonunion tissues were 0.3191±0.0369 and 0.1373±0.0423,respectively,with no significant difference between the two types of tissues (both P＞0.05).There was also no significant difference in patients' age and bone nonunion time between them (all P＞0.05).Conclusion:There is no significant difference in osteogenic potential between the hypertrophic nonunion tissues and the atrophic nonunion tissues.

Intervention effect and mechanism of polyguanylic acid on pulmonary fibrosis in silica-exposed rats / 中国职业医学

OBJECTIVE: To observe the intervention effect of polyguanylic acid( Poly G) on silicosis fibrosis in rats and to explore its possible mechanism. METHODS: Specific pathogen free adult male SD rats were randomly divided into 6 groups:control group,silicosis model group and 4 intervention groups( intervention group 1,2,3 and 4),with 5 rats in each group. Except for the control group,the other 5 groups were treated with 1. 0 m L silica suspension( 50. 0 g / L mass concentration) by intratracheal intubation,four intervention group was given by intraperitoneal injection of 1,2,3 and 4doses of Poly G at 2. 5 mg / kg body weight after establishing the model for 1 to 21 days. All rats were sacrificed 28 days after silicosis model establishment. Lung pathological changes were observed and the pulmonary fibrosis was evaluated by Ashcroft scores. The expression of Macrophage receptor with collagenous structure( MARCO),transforming growth factor-β1( TGF-β1),E-cadherin,Vimentin and α-smooth muscle actin( α-SMA) protein were detected by western blot.RESULTS: In the model group,a large number of dust cell aggregates were found in the alveolar cavity and diffuse collagen deposition appeared in the pulmonary interstitial,indicating that silicosis model was successfully constructed. The alveolar structure of the single dose intervention group was integral and the degree of fibrosis was significantly less than that of the silicosis model group. Compared with the control group,MARCO,TGF-β1,Vimentin and α-SMA expression levels of silicosis model group were increased,the expression level of E-cadherin decreased, the difference was statistically significant( P < 0. 05). Compared with the silicosis model group,TGF-β1,Vimentin and α-SMA expression levels of single dose intervention group decreased,E-cadherin expression level increased,the difference was statistically significant( P < 0. 05). The dose-response relationship could not perceived between the Poly G intervention times and the Ashcroft scores or the protein expression levels of MARCO,TGF-β1,E-cadherin,Vimentin and α-SMA respectively. CONCLUSION: Poly G can effectively reduce lung inflammation and fibrosis in rats. The effect of single dose intervention( 2. 5 mg / kg body weight,intraperitoneal injection) at the first day after silica exposure is the best. The mechanism of action may be related to the low-does Ply G which can inhibit the epithelial-mesenchymal transition and then result in the decrease of collagen synthesis.

The role of thrombospondin-1 in corneal wound healing / 中华实验眼科杂志

Thrombospondin-1 (THBS-1),a kind of extracellular matrix proteins,whose biological action played an important role in corneal wound healing has became a research highlight.The currently research findings showed that THBS-1 could promote the healing of epithelium,stroma and endothelium through activating the transforming growth factor-β1 (TGF-β1) which can accelerate cell proliferation,promote stroma forming and inducing cell migration.It is worthful in clinical treatment of all kinds of corneal wound healing.Now we summarized the research developments which have been acquired in the field recently in this article.

Interleukin 10 (IL10) and transforming growth factor 1 (TGF1) gene polymorphisms in persistent IgE-mediated cow's milk allergy

OBJECTIVES: The aim of this cross-sectional study was to evaluate whether interleukin 10 (IL10) and transforming growth factor β1 (TGFβ1) gene polymorphisms were associated with persistent IgE-mediated cow's milk allergy in 50 Brazilian children. The diagnostic criteria were anaphylaxis triggered by cow's milk or a positive double-blind, placebo-controlled food challenge. Tolerance was defined as the absence of a clinical response to a double-blind, placebo-controlled food challenge or cow's milk exposure. METHOD: The genomic DNA of the 50 patients and 224 healthy controls (HCs) was used to investigate five IL10 gene polymorphisms (-3575A/T, -2849A/G, -2763A/C, -1082G/A, -592C/A) and one TGFβ1 polymorphism (-509C/T). RESULTS: Among the five IL10 polymorphisms analyzed, homozygosis for the G allele at the -1082 position was significantly higher in the patients compared with the healthy controls (p = 0.027) and in the persistent cow's milk allergy group compared with the healthy controls (p = 0.001). CONCLUSIONS: Homozygosis for the G allele at the IL10 -1082G/A polymorphism is associated with the persistent form of cow's milk allergy. .

Hepatocyte growth factor-induced amelioration in renal interstitial fibrosis is associated with reduced expression of -smooth muscle actin and transforming growth factor-1.

Several studies have shown that hepatocyte growth factor (HGF) ameliorates renal interstitial fibrosis, but the mechanism is not fully clear. This study was designed to examine whether HGF can relieve renal interstitial injury in 5/6 nephrectomized rats, and to confirm whether this function was associated with decrease in -smooth muscle actin (-SMA) and transforming growth factor-beta1 (TGF-1) expression. The animals were randomized into 8 groups comprising 6 animals (n = 6) each: control (group I), PCI-neo (group II, 900 μg), sham-operation (group III，not nephrectomy), model or 5/6 nephrectomy group (group IV), lotensin group (an angiotensin converting enzyme inhibitor, group V, 0.6 mg/100 g/day for 5 weeks), low-dose PCI-neo-HGF group (group VI, 690 μg), high-dose PCI-neo-HGF group (group VII, 1380 μg) and lotensin + high-dose PCI-neo-HGF group (group VIII, 0.6 mg/100 g/day for 5 weeks, 1380 μg). The animals were sacrificed in the 5th week after 5/6 nephrectomy. The specimens of kidneys were used for pathological examination (hematoxylin-eosin staining), detection of -SMA and TGF-1 mRNA (Reverse transcriptase-polymerase chain reaction) and protein (Western blot and immunohistochemistry) expression. The results showed that in 5/6 nephrectomized rats blood urea nitrogen (BUN), serum creatinine (CRE) and 24 h urinary albumin excretion (UAE) were increased, renal interstitium was injured seriously and -SMA, TGF-1 mRNA and protein expression were elevated compared with those of control. The above changes were ameliorated and -SMA and TGF-1 expression was reduced by both PCI-neo-HGF and lotensin. The lotensin + high-dose PCI-neo-HGF group rats exhibited the most significant therapeutic effect both in decreasing the BUN, CRE and 24 h UAE and in relieving renal interstitial injury. In conclusion, the study demonstrated that HGF can relieve renal interstitial injury and this protection was associated with down-regulation of –SMA and TGF-1 expressions.

Study on the effect of microparticle platelet frozen-powder on the healing of skin wound in mouse / 中国输血杂志

0.05). Levels of TGF-?1 and PDGF in microparticle platelet frozen powder were higher than those in fresh platelet(P

Protective Effect of the Extract of Ginkgo biloba on the Kidney in Diabetic Rats / 中国药房

OBJECTIVE:To investigate the effect of extract of Ginkgo biloba(EGb) on levels of Endothelin-1(ET-1) and transforming growth factor ?1(TGF-?1) in renal tissue of diabetic rats.METHODS:Twenty-four male Sprague-Dawley rats were randomly divided into three groups:normal control group,diabetic model group and EGb-treated group.The levels of blood glucose,insulin,total cholesterol(TC),total triglycerides(TG),creatinine clearance(Ccr),urinary albumin excretion(UAE),and urinary ?2-MG were measured after 8-week corresponding treatment.Expression of SET-1,UET-1,and RET-1 was examined by radioimmunoassay technique.Expression of STGF-?1 and UTGF-?1 in serum and urine was examined by enzyme linked immunosorbent assay(ELISA).RESULTS:The concentrations of blood glucose,blood insulin,TC and TG increased significantly in diabetic group,which were down-regulated by EGb.Levels of ET-1 and TGF-?1.in both blood and urine and the ET-1 level in renal tissues were significantly higher in diabetic model group than in normal control group(P

The Role of Transforming Growth Factor-?_1 in the Proliferation of Cardiac Fibroblasts Induced by Chymase / 中华高血压杂志

Objective To investigate the effect of chymase on the proliferation of rat cardiac fibroblasts (CFs) and the role of transforming growth factor-?1 (TGF-?_1).Methods Cultured CFs of neonatal SD rats were isolated by trypsinization.Cell number and DNA synthesis were evaluated by MTT assay (A_(490) value) and [~3H]-deoxythy- midine [~3H]-TdR incorporation.The mRNA expression of TGF-?_1 in CFs was determined by RT-PCR.Results Chymase increased CFs numbers and [~3H]-TdR incorporation in a dose-dependent manner.The A_(490) value of CFs stimulated by 15,30 and 60 ng/mL chymase was 0.263?0.033,0.348?0.031 and 0.387?0.026,respectively, which were all significantly higher than that of control (0.201?0.019,P

Effect of Shuxuetong injection on the expressions of TGF-?_1 and Col-IV in renal tissues in diabetic rats / 重庆医科大学学报

Objective:To investigate the effect of Shuxuetong injection on the urine protein excretion changes and expressions of transforming growth factor ?(1TGF-?1)and collegen type Ⅳ(Col-Ⅳ)in renal tissues in diabetic rats.Methods:Diabetic nephropathy was induced by intraperitoneal injection of streptozotocin(STZ).Rats were randomly divided into control group,untreated DN group,Shuxuetong group and Benazepril group.Urinary albumin excretion rate(UAER),?2-microglobulin(?2-MA)and KW/BW(kidney weight/body weigh)were determined 8 weeks later.The expressions of TGF-?1 and Col-Ⅳ in renal tissues were determined by using immunohistochemical methods.Results:After interference,compared with the untreated DN group,UAER,?2-MA,KW/BW in the Shuxuetong group were significantly down-regulated(P

Up-expression of vascular endothelial growth factor in human peritoneal mesothelial cells induced by ovarian carcinoma cells / 基础医学与临床

Objective To investigate the impact of ovarian carcinoma cells on the expression of vascular endothelial growth factor(VEGF) in human peritoneal mesothelial cells(HPMC). Methods HPMC was incubated with ovarian carcinoma cell line in SKOV3 conditioned medium(SKOV3-CM) for(3～ 72 hrs ). RT-PCR was used to detect VEGF gene expression. VEGF protein expression in cell lysates was assessed by ELISA. HPMC was cultured for 24 hrs in SKOV3-CM or SKOV3-CM with anti-transforming growth factor?1(TGF-?1)neutralizing antibody,and the changes in VEGF gene and protein expression were examined. Results VEGF mRNA and protein were detected in HPMC without stimulation of SKOV3-CM. SKOV3-CM significantly induced HPMC VEGF mRNA and protein expression in a time-dependent manner and the induction started 6h(for mRNA) and 12 h (for protein) after the treatment(P

The effects of ammonium perchlorate (AP) on pulmonary fibrosis / 中华急诊医学杂志

Objective To explore the effects of AP on pulmonary fibrosis. Methods Alveolar macrophages (AM) were treated by AP for 24 hours. Pulmonary fibroblasts (FB) were cultured with the supernatant of AM medium. The protein of transforming growth factor beta 1 (TGF-?1) of AM, the proliferative activity and hydroxyproline (Hyp) content of FB were examined. Rats were treated with AP by intratracheal instillation and sacrificed at 3 days. The TGF-?1 mRNA content in the lung was examined. Results The positive staining macrophages in low and high AP groups and the quantity of TGF-?1 in high AP group were obviously higher than those in the control group (P

Expression of transforming growth factor-1 in bone regeneration after the implantation of particulate dentin and plaster of paris

PURPOSE: This study was performed to investigate the expression of the transforming growth factor (TGF)-1, in a rat calvarium defect model using particulate dentin and/or plaster of Paris, and correlate the bone regeneration process with the histologic events. MATERIALS AND METHODS: Thirty-two Sprague-Dawley rats were divided into 4 groups of 8 animals each. A 1.0 cm-sized calvarial defects were made and the defect was filled with different graft materials as follows : Group A, the defects were filled with a mixture of particulate dentin and plaster of Paris with a 2:1 ratio; Group B, the defects were filled with plaster of Paris only; Group C, defects were filled with particulate dentin only; Group D, untreated control group. The animals were sacrificed by 1, 2, 4, 8 weeks after implantation. Excised wound tissues were processed for histology, immunohistochemistry and RT-PCR for the analysis of TGF-1 expression. RESULTS: Gene expression of TGF-1 was detected for all experimental groups. The highest gene expression was observed in the specimen taken at the first week after implantation in Group A. According to the histologic and immunohistochemical studies, TGF- 1 positive osteoblast-like cells were found in the early stage of healing after the implantation of particulate dentin and plaster of Paris. CONCLUSION: These findings suggest that TGF-1 may be related to new bone formation at the early healing process after the implantation of particulate dentin and plaster of Paris.

Effect of allergen on function of peripheral blood CD4+T cells in patients with asthma / 上海第二医科大学学报

Objective To investigate the relationship among the proliferation of CD4+T cells, the intracellular levels of interleukin-10 (IL-10) and transforming growth factor-?1(TGF-?1), and allergic bronchial asthma. Methods Dermatophagoides farinae antigen were prepared as allergen. Twenty-five patients with asthma and 15 healthy individuals were enrolled and divided into blank control group, allergen group and self control group, respectively, after venous blood sample collection. The proliferation of CD4+T cells and the distributions of CD4+/IL-10+, CD4+/TGF-?+1 and CD4+/IL-10+/TGF-?+1 were measuredby flow cytometry (FCM). Results The distributions of CD4+/IL-10+, CD4+/TGF-?+1 and CD4+/IL-10+/TGF-?+1 could hardly be detected in the peripheral blood samples of the blank controls of the patients with asthma and healthy ones. In the allergen group of the healthy individuals, the peripheral blood CD4+T cells were significantly proliferated, and the proportions of CD4+T cells andCD4+/IL-10+ cells were much higher than the self control group, while there was no significantly increase in the proportions of CD4+/TGF-?+1 and CD4+/IL-10+/TGF-?+1 subgroups. In the allergen group of those with asthma, the proportions of peripheral blood CD4+, CD4+/IL-10+, CD4+/TGF-?+1 and CD4+/IL-10+/TGF-?+1 cells were not found significantly increased compared with those self controls. After being activated by allergen, the proportion of peripheral blood CD4+/IL-10+ cells was significantly lower in the patients with asthma than the healthy individuals(P

Effect of IFN-? on Phenotypic Transition of Human Tenon's Conjunctival Capsular Fibroblast / 上海第二医科大学学报

Objective To explore the effect of interferon-?(IFN-?)on phenotypic transition of human Tenon’s conjunctival capsular fibroblast(HTCF). Methods Cultured HTCF derived from 4 operated human cataracts was induced for 48 hours in absence or presence of IFN-? and/or transforming growth factor-?1(TGF-?1). Then immunocytochemistry and Western blot technology were used to detect the ?-smooth muscle actin(?-SMA)expression and identificate the cell phenotype. Results In contrast to normal HTCF, IFN-?(10 ng/mL) inhibited the expression of ?-SMA(P

Study Progress on Relationship of Transforming Growth Factor-?_1 and Airway Remodelig in Asthma / 实用儿科临床杂志

Transforming growth factor-?1 (TGF-?1) was reported to play an important role in the pathogenesis of asthma in many stu-dies.It can stimulate the growth of fibroblast and give rise to fibrosis of lung tissues.Many scholars think that TGF-?1 is closely related to airway remodeling.It is a breakthrough to aim at TGF-?1 to find a way to intervene remodeling in asthma.Glucocorticoid (Dexamethasone and Budesonide) can have an impact in some extent to airway remodeling.In addition,interferon-? and some traditional chinese medicine such as ligustrazine,tripterygium wilfordii and ginkgo maybe effective,they all may ameliorate the progression of airway remodeling following redu-cing partial airway TGF-?1 expression.

Expressions of Transforming Growth Factor-?1 and Connective Tissue Growth Factor in Viral Myocarditis and Intervention Effect of Valsarta on Them / 实用儿科临床杂志

Objective To explore the expressions of transforming growth factor(TGF)-?1 and connective tissue growth factor(CTGF) in viral myocarditis(VM) and the intervention effect of valsarta on them.Methods Fifty-four male Balb/c mice were randomly divided into 3 groups:control group,treatment group and model group.Model group and treatment group established VM model by intraperitoned injecting 0.1 ml CVB3.They were killed on days 7,14 and 28,respectively.Histological cross sections of heart were stained with hematoxylin-eosin and myocardial histopathplogic scores were counted under optical microscope.The expressions of typeⅠand Ⅲ myocardial collagen,TGF-?1 and CTGF were examined by immunohistochemical method and analyzed by using SPSS 11.5 software.Results Compared with control group,histopathplogic scores,the expressions of type Ⅰand Ⅲ myocardial collagen,TGF-?1 and CTGF increased markedly in model group (Pa

Intervention Effect of Silymarin Attenuate Tubulointerstitial Fibrosis in Rats with Unilateral Ureteral Obstruction / 实用儿科临床杂志

Objective To observe the influence of silymarin on expressions of transforming growth factor-?1(TGF-?1) and collagen type Ⅲ(Col Ⅲ) in rats with experimental tubulointerstitial fibrosis(TIF) induced by unilateral ureteral obstruction(UUO).Methods Se-venty-two Sprague-Dawley rats were randomly divided into 3 groups:sham operation group(n=24),model group(n=24) and silymarin treatment group(n=24).The rats in model group and treatment group were operated by ligation of left-ureter.The rats in sham opera-tion group operated by dissociating left-ureter were taken as controls.Rats in treatment group were fed with silymarin [30 mg/(kg?d)]24 h before operation,and rats in model group and sham operation group were treated by intragastric administration with the same volume nomal saline at the same time.Eight rats were killed at day 7,14,and 21,respectively.The score of TIF changes,histologically,was evaluated under light microscope.Expressions of TGF-?1 and Col Ⅲ in tubulointerstitial tissue in 3 groups were investigated by immunohistochemistry.Results 1.The TIF pathological index in treatment group was less than that in model group,but more than sham operation group at day 14 and 21(Pa

Effect of Adenovirus Type 3I,7b on Expressions of mRNA and Protein of Transforming Growth Factor-Beta 1 in Human Embryonic Lung Fibroblast Cells / 实用儿科临床杂志

Objective To study the effect of adenovirus type 3I,7b on the expressions of mRNA and protein of transforming growth factor-beta 1(TGF-?1) in human embryonic lung fibroblast cells.Method The expression of mRNA and protein of TGF-?1 were determined in human embryonic lung fibroblast cells before and after being infected by adenovirus type 3I,7b and in normal fibroblast cells with enzyme-linked immunosorbent assay and in situ hybridization.Results The mRNA and protein of TGF-?1 expression in human embryonic lung fibroblast cells increased siginificantly after being infected by adenovirous type 3I,7b compared with those in normal fibroblast cells(Pa0.05).Conclusion Lung fibroblast cells and TGF-?1 may play some roles in pathophysiological processes of viral pneumonia.