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1.
J Ethnobiol Ethnomed ; 19(1): 50, 2023 Nov 02.
Article in English | MEDLINE | ID: mdl-37919763

ABSTRACT

BACKGROUND: The hot-cold classification system for things and concepts is widely used by many human groups in Mexico. We conducted a comprehensive review to understand the history, themes, and distribution of this system. METHODS: We analyzed publications based on field work in Mexico, considering publication date, research approach, study depth, and conceptual domains. We identified the ethnic groups that use the system and the places where they live. A map illustrates the geographic and cultural distribution of the system. RESULTS: The hot-cold system has been documented in 101 academic publications spanning almost a century, particularly for traditional medicine and food. Initially dominated by anthropological studies, ethnobotanists have increasingly contributed to the research. The hot-cold system is utilized by at least 56 indigenous ethnic groups (81% of the total) and mestizos (whose primary or sole language is Spanish) across most of Mexico. DISCUSSION: Anthropologists laid the foundation for understanding the hot-cold system, on which current ethnobotany builds. However, there are still knowledge gaps, for example on some domains (human beings, landscape) and on patterns by regions or linguistic families. The geographic and cultural distribution presented here is approximate, as group ethnicity is imprecise. CONCLUSIONS: The hot-cold system is widely applied in Mexico, although some variations exist. Further exploration of understudied domains, and variations between ethnic groups and regions, would contribute to a comprehensive explanation of this interconnected worldview.


Subject(s)
Plants, Medicinal , Humans , Mexico , Medicine, Traditional , Ethnobotany , Traditional Medicine Practitioners
2.
Ecol Food Nutr ; 62(5-6): 308-333, 2023 Nov 02.
Article in English | MEDLINE | ID: mdl-37791736

ABSTRACT

Traditional foods (TFs) hold increasing global relevance due to their potential to address health and dietary challenges. This study explores TF consumption and patterns in a middle-income country's general population. Using 2017 Ecuadorian highlands survey data, we identified four consumption clusters with distinct TF preferences. Chi-square tests identified variations in independent variables across clusters. Poisson regression models highlighted city, age, education, and food habits as independent predictors of TF-based clusters. Our findings broaden TF importance to nutrition beyond specific populations. Understanding consumption patterns and socioeconomic links supports nuanced public health strategies to tackle contemporary health, social equity, and sustainability issues.


Subject(s)
Feeding Behavior , Food Preferences , Humans , Ecuador , Diet , Food , Consumer Behavior
3.
Foods ; 10(10)2021 Oct 09.
Article in English | MEDLINE | ID: mdl-34681439

ABSTRACT

Mexico is one of the main regions of the world where the domestication of numerous edible plant species originated. Its cuisine is considered an Intangible Cultural Heritage of Humanity and ferments are important components but have been poorly studied. Traditional fermented foods are still diverse, but some are endangered, requiring actions to promote their preservation. Our study aimed to (1) systematize information on the diversity and cultural history of traditional Mexican fermented beverages (TMFB), (2) document their spatial distribution, and (3) identify the main research trends and topics needed for their conservation and recovery. We reviewed information and constructed a database with biocultural information about TMFB prepared and consumed in Mexico, and we analyzed the information through network approaches and mapped it. We identified 16 TMFB and 143 plant species involved in their production, species of Cactaceae, Asparagaceae, and Poaceae being the most common substrates. Microbiological research has been directed to the potential biotechnological applications of Lactobacillus, Bacillus, and Saccharomyces. We identified a major gap of research on uncommon beverages and poor attention on the cultural and technological aspects. TMFB are dynamic and heterogenous foodscapes that are valuable biocultural reservoirs. Policies should include their promotion for conservation. The main needs of research and policies are discussed.

4.
Food Res Int ; 147: 110482, 2021 09.
Article in English | MEDLINE | ID: mdl-34399478

ABSTRACT

In Mexico, close to 200 fermented products have been described, of which, approximately 20 are beverages. They were obtained through rustic and ancestral fermentation methods by different indigenous Mexican communities; most of them were used in ceremonies, agricultural work, and other occasions. For their elaboration, different substrates obtained from plants are used, where uncontrolled and low-scale spontaneous anaerobic fermentation occurs. In Mexico, some of these products are considered as nutritional sources and functional beverages; the study of those products has revealed the presence of multiple compounds of biological importance. Additionally, elder generations attribute healing properties against diverse illnesses to these beverages. The aim of this review is to highlight the available information on twelve traditional Mexican fermented beverages, their traditional uses, and their fermentation processes along with toxicological, chemical, nutritional, and functional studies as seen from different areas of investigation. In the literature, pulque, cocoa, and pozol were the beverages with the greatest amount of described health properties; sendechó and guarapo were less characterized. Polyphenols, gallic and ferulic acid, anthocyanins and saponins were the most abundant molecules in all beverages. Finally, it is important to continue this research in order to determine the microorganisms that are involved in the fermentation process, as well as the organoleptic and beneficial properties they lend to the traditional Mexican fermented beverages.


Subject(s)
Anthocyanins , Fermented Foods , Beverages , Fermentation , Mexico
5.
Ann Hum Biol ; 48(2): 110-118, 2021 Mar.
Article in English | MEDLINE | ID: mdl-33779426

ABSTRACT

BACKGROUND: Dietary changes, especially declines in traditional food diversity and increases in the consumption of processed foods, have previously been shown to increase the risk of developing cardiovascular diseases. AIM: We evaluated the prevalence and risk factors for metabolic syndrome in four Awajún communities in the Peruvian Amazon. SUBJECTS AND METHODS: A total of 222 participants answered a socioeconomic questionnaire, a 24-h food recall, and completed a physical examination for metabolic syndrome diagnosis. A Poisson regression with robust variance was used in the statistical analysis of risk factors for metabolic syndrome. RESULTS: We found an overall prevalence of 24% for metabolic syndrome. Being female, increased age and body mass index were significant risk factors for metabolic syndrome. Surprisingly, risk more than doubled with the ratio of traditional foods. However, this study revealed that there are fewer traditional foods being consumed in this study compared to prior studies among the Awajún. CONCLUSION: We suggest that the unexpected relationship between traditional food consumption and metabolic syndrome may be due to substantial reductions in the diversity of traditional foods. Currently available traditional foods are primarily high in carbohydrates and are supplemented with cheap, non-perishable, and carbohydrate heavy market food items.


Subject(s)
Diet/classification , Health Status , Indians, South American/statistics & numerical data , Metabolic Syndrome/epidemiology , Adult , Female , Humans , Male , Metabolic Syndrome/etiology , Middle Aged , Peru/epidemiology , Prevalence , Risk Factors , Young Adult
6.
Food Chem ; 350: 129241, 2021 Jul 15.
Article in English | MEDLINE | ID: mdl-33601092

ABSTRACT

The Araucaria araucana kernels are a traditional food in southern Chile and Argentina. The aim of this work was to determine the composition of the phenolic-enriched extracts (PEEs) of the boiled kernels as well as their antioxidant capacity, inhibitory activity on metabolic syndrome-associated enzymes and effect on postprandial oxidative stress in a simulated gastric digestion model. The PEEs composition was assessed by HPLC-DAD-MS/MS. The main PEEs constituents were catechin and epicatechin in the unbound form, while hydroxybenzoic acids occurred mainly in the bound form. The unbound phenolics from boiled kernels showed significant correlations with DPPH, FRAP, TEAC (Pearson's r of 0.481, 0.331 and 0.417, respectively) and lipid peroxidation (r = 0.381) and were more active than the bound phenolics. The extracts were highly active against α-glucosidase (IC50: 0.33-3.15 µg/mL) and reduced lipoperoxidation. Traditional processing increases the flavan-3-ol content. Our results suggest that this traditional food has potential health promoting properties.


Subject(s)
Antioxidants/pharmacology , Araucaria araucana/chemistry , Glycoside Hydrolase Inhibitors/pharmacology , Antioxidants/chemistry , Chile , Flavonoids/analysis , Oxidative Stress , Tandem Mass Spectrometry
7.
Ciênc. Saúde Colet. (Impr.) ; Ciênc. Saúde Colet. (Impr.);25(7): 2519-2528, Jul. 2020.
Article in English, Portuguese | LILACS, Coleciona SUS, Sec. Est. Saúde SP | ID: biblio-1133062

ABSTRACT

Resumo Este artigo analisa as dimensões sociais e culturais da alimentação abordadas nas diretrizes alimentares da segunda edição do Guia Alimentar para a População Brasileira (GAPB), contrapondo-o com a sua primeira edição. Trata-se de um estudo qualitativo. Empregamos a análise de discurso Pecheutiana. O universo de estudo são a primeira e a segunda edição do GAPB. A análise dos dados utilizou três etapas: identificação de textos similares e diferentes dos materiais; elucidação das dimensões sociais e culturais da alimentação nos materiais; e análise sistemática com contraposição dos discursos emergentes em ambos os GAPBs. Enfatizamos que na segunda edição os referenciais teóricos - estudos epidemiológicos, clínicos, sociológicos, antropológicos e saberes populares - e a utilização da classificação de alimentos NOVA favoreceram o desenvolvimento de diretrizes alimentares mais holísticas que abordam os padrões de alimentação e refeição, as práticas culinárias, o ato de comer e a comensalidade. Conclui-se que a segunda edição do GAPB possibilita aos profissionais de saúde e à população uma compreensão do alimento enquanto parte concreta da vida dos indivíduos e coletividade, extrapolando sua dimensão fisiológica e biológica.


Abstract This article analyzes the social and cultural dimensions of food addressed in the dietary guidelines of the second edition of the Dietary Guideline for the Brazilian Population (DGBP) in contrast to its first edition. This is a qualitative study. We employ the Pecheutian discourse analysis. The study universe is the first and second edition of DGBP. Data analysis used three steps: identification of similar and different texts of these materials; elucidation of the social and cultural dimensions of food in these materials; and systematic analysis with contrasting emerging discourses in both DGBPs. We emphasize that in the second edition the theoretical references—epidemiological, clinical, sociological, anthropological studies, and popular knowledge—and the use of the NOVA food classification favored the development of more holistic dietary guidelines that address food and eating patterns, culinary practices, the act of eating and the commensality. It is concluded that the second editions of DGPB allows health professionals and the population an understanding of food as something concrete in the life of individuals and collectivity, extrapolating its physiological and biological dimension.


Subject(s)
Humans , Nutrition Policy , Food , Brazil , Qualitative Research , Feeding Behavior
8.
Curr Dev Nutr ; 4(5): nzaa070, 2020 May.
Article in English | MEDLINE | ID: mdl-32352043

ABSTRACT

BACKGROUND: Over the past several decades, rural and indigenous populations in Latin America have experienced abrupt and profound transformations in their lifestyles and economies, many having remarkable health consequences. Yet, these changes have had heterogeneous effects on the population's biology in different local contexts. OBJECTIVES: The primary goal was to characterize the nutrition transition and biomarkers of noncommunicable diseases (NCD) risk in 2 Chilean indigenous populations that have had divergent histories of subsistence strategies (agropastoralism compared with hunter-gathering) in the last few millennia and live in contrasting environments, and to identify context-specific factors driving the nutrition and epidemiological transitions. METHODS: One-hundred-and-ninety (90 Pehuenche and 100 Atacameño) participants aged 18-87 y completed demographic, food-frequency, and physical activity questionnaires as well as measurements of some NCD risk biomarkers: blood pressure, weight, height, body fat percentage, waist circumference, blood total cholesterol, HDL cholesterol, triglycerides, and glucose. Framingham risk scores (FRSs) were calculated based on age, sex, total cholesterol, HDL cholesterol, systolic blood pressure, smoking, diabetes status, and hypertension medication. RESULTS: Few differences in dietary composition and physical activity patterns were observed between the 2 populations. Multivariate analyses showed no differences between the 2 populations in any of the individual NCD risk biomarkers or FRSs after adjusting for age, sex, time since last meal, food insecurity in childhood, ultraprocessed food consumption, and physical activity. CONCLUSIONS: Despite contrasting ecological and historical contexts, the 2 groups are converging into similar processes of market and wage-labor integration and transitioning to a Western diet high in processed and nonlocal foods, although some aspects of their "traditional" foodways are still in practice. The frequency of individuals exhibiting NCD biomarkers "at-risk" is relatively high and corresponds to other populations that have gone through nutrition transition. Furthermore, none of these biomarkers or FRSs differed between the 2 populations, suggesting a homogenization in the NCD risk factors.

9.
Crit Rev Food Sci Nutr ; 60(14): 2393-2404, 2020.
Article in English | MEDLINE | ID: mdl-31366209

ABSTRACT

Due to rapid international market development, there is a strong and urgent need to assess the safety of the novel food, Ilex guayusa. Guayusa has been consumed for centuries in the western Amazon as an herbal tea, and novel food regulation provide a detailed framework for safety assessment of novel foods with such a history of use. This study reviews guayusa's taxonomy, chemical composition, toxicology, ethnobotany, and history of safe use as key elements of a robust novel food safety assessment. Guayusa is a product of traditional agricultural systems with a continuous history of consumption in Ecuador. Its known chemical composition appears to present no greater risk to human health than existing teas such as green tea or yerba mate, although our understanding of guayusa's chemical profile is still nascent, requiring further investigation. Broad consumption of guayusa is not associated with a history of adverse effects or product safety notifications. Biochemical and phytochemical studies have profiled its nutritional content, metabolite composition, and bioactivity, validating guayusa's antioxidant and stimulant properties. In conclusion, guayusa leaves have a well-documented chemical composition and history of safe use, which are key considerations for authorization as a novel food in the EU.


Subject(s)
Antioxidants/chemistry , Antioxidants/pharmacology , Food Safety , Ilex guayusa/chemistry , Humans , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Leaves/chemistry
10.
Rev. chil. nutr ; 46(5): 593-605, oct. 2019. tab
Article in Spanish | LILACS | ID: biblio-1042700

ABSTRACT

Sólo un 30% de los hogares en México, presentan seguridad alimentaria, es decir, un gran porcentaje de los hogares y por ende de la población no satisfacen las necesidades básicas diarias de macro y micronutrimentos. Los quelites, que se definen como plantas silvestres comestibles (hojas, tallos y flores) son una fuente accesible, continua, económica y adecuada de nutrimentos. Sin embargo, su consumo ha disminuido por modificaciones en las preferencias alimentarias, derivadas de los cambios en estilos de vida. El objetivo del presente trabajo fue valorizar a los quelites como fuente de alimento. Los quelites, aportan proteínas, aminoácidos, minerales (Ca, Mg, Zn), vitaminas (E, C) y fibra. Además, son una excelente fuente de compuestos bioactivos, como ácidos fenólicos (ácido cafeico, ferúlico) y flavonoides (quercetina, kaempferol, espinacetina), carotenoides, ácido α-linolénico y betalainas, que presentan elevada actividad antioxidante. Su consumo habitual se ha relacionado con beneficios a la salud, tales como efectos antitumorales, antihiperlipidémicos y antidiabéticos. Los quelites, además de estar disponibles en forma silvestre, forman parte de las tradiciones culinarias de México, incorporados de forma cruda y cocida en los platillos regionales. Por lo tanto, la revalorización y reincorporación de los quelites en la dieta, puede coadyuvar a cubrir las necesidades nutrimentales, en poblaciones con poco acceso o inseguridad alimentaria, además de contribuir a proporcionar efectos adicionales a través de sus compuestos bioactivos.


Only 30% of households in Mexico present food security, which means a large percentage of households and the population does not meet their needs in terms of macro and micronutrients. Thus, quelites, which are defined as wild edible plants, are an accessible, continuous, economical and adequate source of nutrients. However, quelite consumption in Mexico has been decreasing in response to changes in food preferences and lifestyles, including increased exposure to hypercaloric foods. Therefore, the aim of this work was to discuss the value of quelites as a food source rich in nutrients, proteins, amino acids, minerals (Ca, Mg, Zn), vitamins (E, C) and fiber. We also discuss how quelites are good source of bioactive compounds, such as phenolic acids (caffeic acid, ferulic acid) and flavonoids (quercetin, kaempferol, spinacetin), carotenoids, α-linolenic acid and betalaines. There use have been mainly related to the anti-tumor, antihyperlipidemic and antidiabetic benefits. We further discuss topics related to the culinary traditions of Mexico and the incorporation of quelites in the raw and cooked form in regional markets. The revaluation and the reincorporation of quelites in the diet can help meet nutritional needs, in addition to possibly providing additional health benefits.


Subject(s)
Humans , Plants, Edible , Chenopodium/chemistry , Portulaca/chemistry , Phytochemicals/analysis , Plants, Medicinal , Food Supply , Mexico
11.
Article in English | MEDLINE | ID: mdl-31484302

ABSTRACT

Certain components of global food security continue to be threatened. Globalization has impacted food patterns, leading to greater homogenization of diets and the standardization of processes of food transformation, both in the countryside and in the cities. In Mexico, this has led to a drop in the use of native corn landraces and in the value associated with traditional practices around their growing and the processing and consumption of tortillas. The aim of this work was to analyze the main characteristics of the handmade comal tortilla system along the rural-urban gradient taking into account: (1) The type of seed and production, (2) manufacturing processes, (3) marketing channels and purpose of sales, and (4) perceptions regarding the quality of the product. Research was conducted on 41 handmade tortilla workshops located in rural areas in the Lake Pátzcuaro Basin and in urban and peri-urban areas of a medium-sized city in Michoacán (Mexico). Results showed that the origin of the grain follows a gradient-like pattern: In rural areas, tortillas are made with local and native corn predominate, while in urban contexts most tortillas come from hybrid corn produced in Sinaloa or Jalisco. There is a generalized preference for white tortillas, but blue tortillas are used for personal consumption in rural areas and as a gourmet product in the city. 100% of the rural workshops make their own nixtamal, while almost 50% of the peri-urban and urban businesses buy pre-made nixtamal dough. Surprisingly, 50% of the rural handmade tortilla workshops admit that they add nixtamalized corn flour and/or wheat flour to their tortilla mix. We conclude that not all handmade comal tortillas are produced equally and, although in rural areas traditions are better preserved, these also have contradictions. We also conclude that it is important to promote the revaluation of agrobiodiversity, traditional gastronomy, and food security without sacrificing quality, nutrition, and flavor.


Subject(s)
Bread , Edible Grain , Food Handling/methods , Zea mays , Flour , Humans , Mexico , Rural Population , Triticum , Urban Population
12.
Antonie Van Leeuwenhoek ; 111(3): 385-399, 2018 Mar.
Article in English | MEDLINE | ID: mdl-29058140

ABSTRACT

The purpose of this work was to analyse the diversity and dynamics of lactic acid bacteria (LAB) throughout the fermentation process in Atole agrio, a traditional maize based food of Mexican origin. Samples of different fermentation times were analysed using culture-dependent and -independent approaches. Identification of LAB isolates revealed the presence of members of the genera Pediococcus, Weissella, Lactobacillus, Leuconostoc and Lactococcus, and the predominance of Pediococcus pentosaceus and Weissella confusa in liquid and solid batches, respectively. High-throughput sequencing (HTS) of the 16S rRNA gene confirmed the predominance of Lactobacillaceae and Leuconostocaceae at the beginning of the process. In liquid fermentation Acetobacteraceae dominate after 4 h as pH decreased. In contrast, Leuconostocaceae dominated the solid fermentation except at 12 h that were overgrown by Acetobacteraceae. Regarding LAB genera, Lactobacillus dominated the liquid fermentation except at 12 h when Weissella, Lactococcus and Streptococcus were the most abundant. In solid fermentation Weissella predominated all through the process. HTS determined that Lactobacillus plantarum and W. confusa dominated in the liquid and solid batches, respectively. Two oligotypes have been identified for L. plantarum and W. confusa populations, differing in a single nucleotide position each. Only one of the oligotypes was detected among the isolates obtained from each species, the biological significance of which remains unclear.


Subject(s)
Beverages/microbiology , Biodiversity , Food Microbiology , Lactobacillales/classification , Lactobacillales/genetics , Computational Biology/methods , Fermentation , High-Throughput Nucleotide Sequencing , Lactobacillales/isolation & purification , Lactobacillales/metabolism , Mexico , RNA, Ribosomal, 16S/genetics
13.
Hig. aliment ; 31(270/271): 85-91, jul.-ago. 2017. tab, ilus
Article in Portuguese | VETINDEX | ID: vti-18503

ABSTRACT

A farinha de mandioca fermentada é um alimento típico da região amazônica brasileira e sua produção ocorre com as seguintes etapas: colheita, descascamento, fermentação (tubérculos são imersos em um tanque com água ou córrego), lavagem, trituração, prensagem, moagem e torrefação. A farinha de mandioca é uma fonte importante de carboidratos na Amazônia, especialmente pelas pessoas de baixa renda. Neste trabalho estudou-se o comportamento físico-químico e microbiológico da farinha de mandioca fermentada comercializada em um mercado de rua e em supermercado. Para tal, 36 quilogramas de farinha de mandioca fermentada foram adquiridos e mantidos sob as mesmas condições ambientais do mercado de rua e do supermercado. Durante dois meses, as amostras foram analisadas quanto à umidade, cinzas acidez total titulável, pH e atividade de água. Os resultados mostraram que o pH e a acidez total titulável foram estáveis, mas a umidade e a atividade de água mudaram e aumentaram significativamente (p > 0,05).(AU)


Fermented cassava flour is a typical food of Amazonia region and its production occurs with following stages: fermentation (unpeeled cassava tubers are immersed in a water tank or stream), peeling, milling and roasting. The cassava flour is an important source of carbohydrates in Amazonia, especially for low-income people. In this work we studied the physical-chemical and microbiological behavior of the fermented cassava flour commercialized in a street market, and at a supermarket, 36 kg of the fermented cassava flour were purchased and maintained under the same environmental conditions of the street market and that of the supermarket. During two months the samples were analyzed for moisture, ash, total titratable acidity, pH and water activity. The results showed that the pH and total titratable acidity were stable, but the moisture and the water activity have changed and increased significantly (p > 0,05).(AU)


Subject(s)
Humans , Flour/analysis , Manihot/chemistry , Manihot/microbiology , Food Storage , Chemical Phenomena , Food Samples , Brazil
14.
Hig. aliment ; 31(270/271): 85-91, 29/08/2017.
Article in Portuguese | LILACS | ID: biblio-848943

ABSTRACT

A farinha de mandioca fermentada é um alimento típico da região amazônica brasileira e sua produção ocorre com as seguintes etapas: colheita, descascamento, fermentação (tubérculos são imersos em um tanque com água ou córrego), lavagem, trituração, prensagem, moagem e torrefação. A farinha de mandioca é uma fonte importante de carboidratos na Amazônia, especialmente pelas pessoas de baixa renda. Neste trabalho estudou-se o comportamento físico-químico e microbiológico da farinha de mandioca fermentada comercializada em um mercado de rua e em supermercado. Para tal, 36 quilogramas de farinha de mandioca fermentada foram adquiridos e mantidos sob as mesmas condições ambientais do mercado de rua e do supermercado. Durante dois meses, as amostras foram analisadas quanto à umidade, cinzas acidez total titulável, pH e atividade de água. Os resultados mostraram que o pH e a acidez total titulável foram estáveis, mas a umidade e a atividade de água mudaram e aumentaram significativamente (p>0,05).(AU)


Subject(s)
Humans , Manihot/microbiology , Manihot/chemistry , Food Storage , Flour/analysis , Brazil , Food Samples
15.
Matern Child Nutr ; 13 Suppl 32017 11.
Article in English | MEDLINE | ID: mdl-29359432

ABSTRACT

Traditional knowledge and practice of Indigenous Peoples related to their food use and well-being is a wealth of information for academic study and for public health nutrition. Despite unique long-evolved heritages of knowledge of ecosystem resources, Indigenous Peoples comprise 15% of the global poor, but only 5% of the world's population, and they experience poverty, discrimination, and poor nutritional health at far greater rates than mainstream populations in their nations of residence. These disparities are unacceptable in all human rights frameworks, and the call to alleviate them resonates through all human development programmes and the United Nations organizations. The scholars contributing to this special issue of Maternal and Child Nutrition describe how gender roles and the right to food for several cultures of Indigenous Peoples can be fostered to protect their unique foods and traditions, providing food sovereignty and food and nutrition security benefits, especially for women and children. Aspects of societal maternal or paternal lineality and locality, division of labour, spirituality and decision-making are described. These factors structure the impact of gender roles with Indigenous worldviews on the dynamics of family food access, its availability and use, and the use of local food biodiversity. Cultures of Indigenous Peoples in Ecuador, Nigeria, Thailand, India, Canada, Japan, and Morocco are discussed. This publication is a work of the Task Force on Traditional, Indigenous and Cultural Food and Nutrition of the International Union of Nutritional Sciences.


Subject(s)
Culture , Diet , Food Supply , Gender Identity , Population Groups , Canada , Child Health , Ecuador , Female , Health Knowledge, Attitudes, Practice , Health Status , Humans , India , Japan , Male , Malnutrition/prevention & control , Morocco , Nigeria , Nutrition Assessment , Nutrition Policy , Public Health , Thailand , Women's Health
16.
J Proteomics ; 111: 139-47, 2014 Dec 05.
Article in English | MEDLINE | ID: mdl-25009144

ABSTRACT

Pozol is a traditional fermented maize dough prepared in southeastern Mexico. Wide varieties of microorganisms have already been isolated from this spontaneously fermented product; and include fungi, yeasts, and lactic- and non-lactic acid bacteria. Pozol presents physicochemical features different from that of other food fermentation products, such as a high starch content, in addition to a low protein content. It is these qualities that make it intractable for protein recovery and characterization. The aim of this study was to develop a methodology to optimize the recovery of proteins from the pozol dough following fermentation, by reducing the complexity of the mixture prior to 2D-PAGE analysis and sequencing, to allow the characterization of the metaproteome of the dough. The proteome of 15day fermented maize dough was characterized; proteins were separated and analyzed by mass spectrometry (LC-MS/MS). Subsequent sequence homology database searching, identified numerous bacterial and fungi proteins; with a predominance of lactic acid bacterial proteins, mainly from the Lactobacillus genus. Fungi are mainly represented by Aspergillus. For dominant genera, the most prevalent proteins belong to carbohydrate metabolism and energy production, which suggest that at 15days of fermentation not only fungi but also bacteria are metabolically active. BIOLOGICAL SIGNIFICANCE: Several methodologies have been employed to study pozol, with a specific focus toward the identification of the microbiota of this fermented maize dough, using both traditional cultivation techniques and culture independent molecular techniques. However to date, the dynamics of this complex fermentation is not well understood. With the purpose to gain further insight into the nature of the fermentation, we used proteomic technologies to identify the origin of proteins and enzymes that facilitate substrate utilization and ultimately the development of the microbiota and fermentation. In this paper we overcome the first general challenge for such studies, obtaining a protein sample with adequate quality capable of representing the system.


Subject(s)
Food Analysis/methods , Proteins/isolation & purification , Proteomics , Starch/chemistry , Zea mays/chemistry , Aspergillus/chemistry , Chromatography, Liquid , Fermentation , Food Microbiology , Lactobacillus/chemistry , Mexico , Microbiota , Proteins/chemistry , Proteome , Tandem Mass Spectrometry , Trypsin/chemistry
17.
Perspect. nutr. hum ; 12(1): 61-74, ene.-jun. 2010. tab
Article in Spanish | LILACS | ID: lil-591518

ABSTRACT

Objetivo: interpretar los aspectos socioculturales y técnico-nutricionales de la alimentación de un grupo de adultos mayores del centro gerontológico Colonia de Belencito. Materiales y métodos: el estudio se fundamentó en el enfoque de investigación cualitativa y se utilizó como perspectiva metodológica la etnografía de tipo particularista o enfocada. Las técnicas para la recolección de la información fueron la observación participante, la entrevista en profundidad, la entrevista semiestructurada y los grupos de discusión. Resultados: entre los principales hallazgos se encontró que el concepto de adulto mayor y los aspectos socioculturales de las directrices políticas que rigen la alimentación en la institución, no corresponden a lo que se propone para un proceso de envejecimiento activo; las especificaciones técnicas para el diseño de la minuta alimentaria se enmarcan en un modelo medicalizado de la alimentación; los adultos mayores manifestaron un gusto marcado por la alimentación tradicional, la cual dentro de los procesos de socialización marca una identidad y un entorno de significaciones alimentarias.


Objective: The main purpose of this research was to determine social and cultural characteristics influencing alimentary and nutritional aspects of elderly people, living in a center care called Colonia de Belencito Medellin. Methods: This is a qualitative study that including different techniques to collect data like direct observational process, semi structured surveys and focus groups method. Results: The main results showed in this study was that elderly concept and socio-cultural characteristics influencing nutrition behaviors in this population, don’t fit with the proposed model for an active and healthy elderly process; the specific techniques to design menus for this specific group of population are submitted to a classical medical nutrition model: elderly people affirmed that they prefer traditional food, because of it is a socializing process that establish cultural identity of alimentary meaning.


Subject(s)
Aged , Feeding Behavior , Nutrition Programs and Policies , Aged , Collective Feeding
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