RESUMO
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.
Assuntos
Aminobutiratos/farmacologia , Herbicidas/farmacologia , Plantas Geneticamente Modificadas , Triticum/genética , Southern Blotting , DNA de Plantas , Resistência a Medicamentos , Glucuronidase/metabolismo , Modelos Genéticos , Plasmídeos/metabolismo , Reação em Cadeia da Polimerase , Fatores de TempoRESUMO
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.(AU)
Assuntos
Aminobutiratos/farmacologia , Herbicidas/farmacologia , Plantas Geneticamente Modificadas , Triticum/genética , Southern Blotting , DNA de Plantas , Resistência a Medicamentos , Glucuronidase/metabolismo , Modelos Genéticos , Plasmídeos/metabolismo , Reação em Cadeia da Polimerase , Fatores de TempoRESUMO
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.
Assuntos
Aminobutiratos/farmacologia , Herbicidas , Plantas Geneticamente Modificadas , Triticum , Southern Blotting , DNA de Plantas , Glucuronidase , Modelos Genéticos , Plasmídeos/metabolismo , Reação em Cadeia da Polimerase , Resistência a Medicamentos , Fatores de TempoRESUMO
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which [quot ]model[quot ] cultivar was used.
