Uso da miltefosina no tratamento clínico de cães com leishmaniose visceral: revisão de literatura / Use of miltefosin in the clinical treatment of dogs with visceral leishmaniosis / Uso de miltefosina en el tratamiento clínico de perros con leishmaniosis visceral: revisión de la literatura

A leishmaniose visceral canina é uma doença de caráter zoonótico, acometendo os seres humanos e diversas espécies de animais silvestres e domésticos. Objetivou-se com o presente estudo realizar uma revisão de literatura sobre o uso da miltefosina no tratamento clínico de cães com leishmaniose visceral. Trata- se de uma revisão de literatura, a qual foi realizada por meio de consultas à periódicos e livros presentes na biblioteca do Cesmac. Foram utilizadas bases de dados como: portal Capes, SCIELO, Google Acadêmico; pesquisa em monografias, teses e dissertações. Causada pelo protozoário Leishmania chagasi, sendo o cão doméstico o principal reservatório desse protozoário. Por representar um problema grave de saúde pública e ser considerada uma doença potencialmente fatal (quando não tratada precocemente e adequadamente), faz- se importante que o clínico esteja familiarizado com os sinais clínicos, exames complementares e principais protocolos terapêuticos, em especial a utilização da miltefosina no tratamento da leishmaniose visceral em cães. Por ser uma zoonose que causa graves problemas de saúde pública e que vem crescendo cada vez mais no Brasil, cabe aos médicos veterinários assumirem o compromisso na conscientização sobre a importância do diagnóstico precoce além de promoverem o bem-estar animal e a saúde pública.(AU)

Canine visceral leishmaniasis is a zoonotic disease, affecting humans and several species of wild and domestic animals. The objective of the present study was to carry out a literature review on the use of miltefosine in the clinical treatment of dogs with visceral leishmaniasis. This is a literature review, which was carried out through consultations with periodicals and books present in the Cesmac library. Databases such as: Capes portal, SCIELO, Google Scholar; research in monographs, theses and dissertations. Caused by the protozoan Leishmania chagasi, with the domestic dog being the main reservoir of this protozoan. As it represents a serious public health problem and is considered a potentially fatal disease (when not treated early and properly), it is important that the clinician is familiar with the clinical signs, complementary exams and main therapeutic protocols, especially the use of miltefosine in the treatment of visceral leishmaniasis in dogs. As it is a zoonosis that causes serious public health problems and that has been growing more and more in Brazil, it is up to veterinarians to make a commitment to raise awareness of the importance of early diagnosis in addition to promoting animal welfare and public health.(AU)

La leishmaniosis visceral canina es una enfermedad zoonótica que afecta a los seres humanos y a varias especies de animales salvajes y domésticos. El objetivo de este estudio fue realizar una revisión bibliográfica sobre el uso de la miltefosina en el tratamiento clínico de perros con leishmaniosis visceral. Se trata de una revisión bibliográfica, que se realizó mediante consultas a publicaciones periódicas y libros presentes en la biblioteca del Cesmac. Se utilizaron bases de datos como: portal Capes, SCIELO, Google Académico; investigación en monografías, tesis y disertaciones. Causada por el protozoo Leishmania chagasi, siendo el perro doméstico el principal reservorio de este protozoo. Dado que representa un grave problema de salud pública y se considera una enfermedad potencialmente mortal (cuando no se trata de forma temprana y adecuada), es importante que el clínico esté familiarizado con los signos clínicos, las pruebas adicionales y los principales protocolos terapéuticos, especialmente el uso de miltefosina en el tratamiento de la leishmaniosis visceral en perros. Siendo una zoonosis que causa graves problemas de salud pública y que viene creciendo cada vez más en Brasil, corresponde a los veterinarios asumir el compromiso de concienciar sobre la importancia del diagnóstico precoz y promover el bienestar animal y la salud pública.(AU)

Utilização da fisioterapia na reabilitação de bezerro com poliartrite e paresia neuromuscular: relato de caso / Use of physiotherapy in the rehabilitation of calves with polyarthritis and neuromuscular paresis: case report / Uso de la fisioterapia en la rehabilitación de un ternero con poliartritis y parisia neuromuscular: reporte de caso

A reabilitação animal na medicina veterinária é um campo recente, porém crescente a cada dia. Neste sentido, a fisioterapia veterinária atua trazendo inúmeros benefícios, como melhoria dos movimentos, redução da dor, edema e outras. Interfere ainda no tempo de recuperação, redução de custos para o proprietário, podendo ser hoje utilizada como um tratamento na recuperação pós-cirúrgica. Desta forma, objetivou-se relatar o uso da fisioterapia em uma bezerra com poliartrite e paresia neuromuscular. O animal apresentou um aumento de volume nas articulações cárpicas e társicas após histórico de onfalite, permanecendo em decúbito esternal por vários dias. O proprietário resolveu aplicar ferro dextrano na região glútea do animal, o que causou uma lesão de nervo isquiático. Foram instituídos protocolos medicamentosos e fisioterápicos, que culminaram no estímulo da marcha, propriocepção, além de hipertrofia muscular. Porém, em virtude de complicações resultantes de onfalite, o animal veio a óbito.(AU)

Animal rehabilitation in veterinary medicine is a recent field, but growing every day. In this sense, veterinary physiotherapy works bringing numerous benefits, such as improved movements, reduced pain, edema and others. Reducing the recovery time, reducing costs for the owner, and today it can be used as a treatment in post-surgical recovery. In this sense, the objective was to report the use of physiotherapy in a heifer with polyarthritis and neuromuscular paresis. The animal showed an increase in volume in the carpal and tarsal joints after a history of omphalitis, remaining in sternal decubitus for several days. The owner decided to apply iron dextran to the animal's gluteal region, which caused an injury to the sciatic nerve. Medicinal and physical therapy protocols were instituted, which culminated in the stimulation of gait, proprioception, in addition to muscle hypertrophy. However, due to complications resulting from omphalitis, the animal died.(AU)

La rehabilitación animal en medicina veterinaria es un campo reciente, pero en crecimiento cada día. En este sentido, la fisioterapia veterinaria actúa aportando numerosos beneficios, como mejora de los movimientos, reducción del dolor, edemas y otros. Disminución del tiempo de recuperación, reducción de costos para el propietario, pudiendo ser utilizado hoy en día como tratamiento en la recuperación posquirúrgica. En ese sentido, el objetivo fue reportar el uso de fisioterapia en una vaquilla con poliartritis y paresia neuromuscular. El animal presentó un aumento de volumen en las articulaciones del carpo y del tarso tras un antecedente de onfalitis, permaneciendo en decúbito esternal durante varios días. El propietario decidió aplicar hierro dextrano en la región glútea del animal, lo que provocó una lesión en el nervio ciático. Se instauraron protocolos farmacológicos y de fisioterapia, que culminaron con estimulación de la marcha, propiocepción, además de hipertrofia muscular. Sin embargo, debido a complicaciones derivadas de la onfalitis, el animal falleció.(AU)

Uso da miltefosina no tratamento clínico de cães com leishmaniose visceral: revisão de literatura

A leishmaniose visceral canina é uma doença de caráter zoonótico, acometendo os seres humanos e diversas espécies de animais silvestres e domésticos. Objetivou-se com o presente estudo realizar uma revisão de literatura sobre o uso da miltefosina no tratamento clínico de cães com leishmaniose visceral. Trata-se de uma revisão de literatura, a qual foi realizada por meio de consultas à periódicos e livros presentes na biblioteca do Cesmac. Foram utilizadas bases de dados como: portal Capes, SCIELO, Google Acadêmico; pesquisa em monografias, teses e dissertações. Causada pelo protozoário Leishmania chagasi, sendo o cão doméstico o principal reservatório desse protozoário. Por representar um problema grave de saúde pública e ser considerada uma doença potencialmente fatal (quando não tratada precocemente e adequadamente), faz-se importante que o clínico esteja familiarizado com os sinais clínicos, exames complementares e principais protocolos terapêuticos, em especial a utilização da miltefosina no tratamento da leishmaniose visceral em cães. Por ser uma zoonose que causa graves problemas de saúde pública e que vem crescendo cada vez mais no Brasil, cabe aos médicos veterinários assumirem o compromisso na conscientização sobre a importância do diagnóstico precoce além de promoverem o bem-estar animal e a saúde pública.

Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models.

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.

Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.(AU)

Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.

Avaliação biométrica e termográfica de cascos de equinos de tração urbana de Maceió, Estado de Alagoas - Brasil / Biometric and termographyc evaluation of equine hoves used to pull wagons in Maceió city/Alagoas state, Brazil / Evaluación biométrica y termográfica de caballos de tracción urbana en Maceió, Estado de Alagoas, Brasil

O presente artigo realizou a avaliação biométrica e termoqráfica dos cascos de equinos de tração urbana de Maceió/AL, atendidos pelo Projeto de Extensão Pró-Carroceiros da UFAL, buscando identificar possíveis desequilíbrios podais em 30 equinos hígidos do município. Desses, 12 eram fêmeas e 18 machos, sendo mensuradas o comprimento da parede lateral do casco, comprimento dorsal da pinça, ângulo da pinça, largura e comprimento de ranilha, largura e comprimento do casco nos quatro membros. A incidência de desequilíbrio do casco nos equinos de tração de Maceió/AL foi alta, sendo relevante a baixa angulação da pinça tanto em membros torácicos quanto pélvicos, além da contração de ranilha. Provavelmente, as técnicas inadequadas de casqueamento e falta de ferrageamento sejam os principais fatores etiológicos dos desequilíbrios nessa população. Em relação à termografia, nota-se valores médios próximos aos encontrados na literatura. Essa pesquisa traz dados inéditos sobre biometria e termografia podal em equinos de tração urbana na realidade da região Nordeste do Brasil, porém são necessários mais estudos, visando a avaliar o efeito da distribuição desigual do peso nos cascos em relação às principais enfermidades do aparelho locomotor, além do estudo termográfico de animais com afecções do casco.(AU)

The present paper carried out the biometric and thermographic evaluation of the horses of urban traction horses of Maceió/AL, attended by the Pro-Carroceiros Extension Project of Federal University of Alagoas, seeking to identify possible foot imbalances in 30 healthy horses of the municipality. Of these, 12 were females and 18 males, the length of the lateral wall of the hull, the dorsal length of the forceps, the angle of the forceps, the width and length of the hull, width and length of the hull in the four limbs were measured. The incidence of hull imbalance in the Maceió/AL traction horses was high, being relevant the low angulation of the tweezers in both thoracic and pelvic limbs, in addition to the contraction of the ranch. Inadequate techniques of casqueamento and lack of feathering are probably the main etiological factors of imbalances in this population. In relation to thermography, we can see average values close to those found in the literature. This research brings unpublished data on biometrics and pedal thermography in urban traction horses in the Northeastern region of Brazil, but further studies are needed to evaluate the effect of unequal weight distribution on hoofs in relation to the main diseases of the locomotor system, besides the thermographic study of animals with hull affections.(AU)

El presente artículo llevó a cabo la evaluación biométrica y termográfica de los caballos de tracción urbana de Maceió/AL, a la que asistió el Proyecto de Extensión Pro-Carroceiros de la UFAL, que busca identificar posibles desequilibrios del pie en 30 caballos sanos del municipio. De estos, 12 eran hembras y 18 machos, se midieron la longitud de la pared lateral del casco, la longitud dorsal de las pinzas, el ángulo de las pinzas, la anchura y la longitud del casco, la anchura y la longitud del casco en las cuatro extremidades. La incidencia de desequilibrio en el casco en los caballos de tracción Maceió/AL fue alta, siendo relevante la baja angulación de las pinzas en las extremidades torácicas y pélvicas, además de la contracción del rancho. Las técnicas inadecuadas de casqueamento y la falta de plumaje son probablemente los principales factores etiológicos de los desequilibrios en esta población. En relación a la termografía, podemos ver valores promediocercanos a los encontrados en la literatura. Esta investigación aporta datos no publicados sobre biometría y termografía de pedal en caballos de tracción urbana en la región noreste de Brasil, pero se necesitan más estudios para evaluar el efecto de la distribución desigual del peso en los cascos en relación con las principales enfermedades del sistema locomotor. Además del estudio termográfico de animales con afecciones del casco.(AU)

Avaliação biométrica e termográfica de cascos de equinos de tração urbana de Maceió, Estado de Alagoas - Brasil / Biometric and termographyc evaluation of equine hoves used to pull wagons in Maceió city/Alagoas state, Brazil / Evaluación biométrica y termográfica de caballos de tracción urbana en Maceió, Estado de Alagoas, Brasil

O presente artigo realizou a avaliação biométrica e termoqráfica dos cascos de equinos de tração urbana de Maceió/AL, atendidos pelo Projeto de Extensão Pró-Carroceiros da UFAL, buscando identificar possíveis desequilíbrios podais em 30 equinos hígidos do município. Desses, 12 eram fêmeas e 18 machos, sendo mensuradas o comprimento da parede lateral do casco, comprimento dorsal da pinça, ângulo da pinça, largura e comprimento de ranilha, largura e comprimento do casco nos quatro membros. A incidência de desequilíbrio do casco nos equinos de tração de Maceió/AL foi alta, sendo relevante a baixa angulação da pinça tanto em membros torácicos quanto pélvicos, além da contração de ranilha. Provavelmente, as técnicas inadequadas de casqueamento e falta de ferrageamento sejam os principais fatores etiológicos dos desequilíbrios nessa população. Em relação à termografia, nota-se valores médios próximos aos encontrados na literatura. Essa pesquisa traz dados inéditos sobre biometria e termografia podal em equinos de tração urbana na realidade da região Nordeste do Brasil, porém são necessários mais estudos, visando a avaliar o efeito da distribuição desigual do peso nos cascos em relação às principais enfermidades do aparelho locomotor, além do estudo termográfico de animais com afecções do casco.

The present paper carried out the biometric and thermographic evaluation of the horses of urban traction horses of Maceió/AL, attended by the Pro-Carroceiros Extension Project of Federal University of Alagoas, seeking to identify possible foot imbalances in 30 healthy horses of the municipality. Of these, 12 were females and 18 males, the length of the lateral wall of the hull, the dorsal length of the forceps, the angle of the forceps, the width and length of the hull, width and length of the hull in the four limbs were measured. The incidence of hull imbalance in the Maceió/AL traction horses was high, being relevant the low angulation of the tweezers in both thoracic and pelvic limbs, in addition to the contraction of the ranch. Inadequate techniques of casqueamento and lack of feathering are probably the main etiological factors of imbalances in this population. In relation to thermography, we can see average values close to those found in the literature. This research brings unpublished data on biometrics and pedal thermography in urban traction horses in the Northeastern region of Brazil, but further studies are needed to evaluate the effect of unequal weight distribution on hoofs in relation to the main diseases of the locomotor system, besides the thermographic study of animals with hull affections.

El presente artículo llevó a cabo la evaluación biométrica y termográfica de los caballos de tracción urbana de Maceió/AL, a la que asistió el Proyecto de Extensión Pro-Carroceiros de la UFAL, que busca identificar posibles desequilibrios del pie en 30 caballos sanos del municipio. De estos, 12 eran hembras y 18 machos, se midieron la longitud de la pared lateral del casco, la longitud dorsal de las pinzas, el ángulo de las pinzas, la anchura y la longitud del casco, la anchura y la longitud del casco en las cuatro extremidades. La incidencia de desequilibrio en el casco en los caballos de tracción Maceió/AL fue alta, siendo relevante la baja angulación de las pinzas en las extremidades torácicas y pélvicas, además de la contracción del rancho. Las técnicas inadecuadas de casqueamento y la falta de plumaje son probablemente los principales factores etiológicos de los desequilibrios en esta población. En relación a la termografía, podemos ver valores promediocercanos a los encontrados en la literatura. Esta investigación aporta datos no publicados sobre biometría y termografía de pedal en caballos de tracción urbana en la región noreste de Brasil, pero se necesitan más estudios para evaluar el efecto de la distribución desigual del peso en los cascos en relación con las principales enfermedades del sistema locomotor. Además del estudio termográfico de animales con afecciones del casco.

Carcinoma de células escamosas em dorso de bovino / Carcinoma of squamous cells in back bovine

Um boi de carroça, criado no Semi-Árido potiguar, foi atendido no HOVET-UFERSA, com aumento de volume localizado na região dorsal. A neoplasia foi diagnosticada clinicamente e confirmada após estudo histopatológico em carcinoma de células escamosas (CCE). Foi tratado por exérese cirúrgica. Para a remoção da massa tumoral utilizou-se bisturi elétrico com de fio de aço acoplado, seguida de uso de serra de Giglle. O paciente permaneceu internado durante 7 dias, sendo medicado, com cicatrização ocorrida por segunda intenção. Recuperado o paciente, com excelente retração cicatricial da ferida cirúrgica, teve alta hospitalar. A conduta de exérese cirúrgica do CCE foi rápida e de fácil execução, contudo,é fundamental a avaliação prévia, visando delinear e identificar a técnica de tratamento mais eficiente e menos invasiva.(AU)

A wagon bull, raised in the potiguar Semi-Arid, was admitted to the HOVET-UFERSA, with a swelling located on its back. This neoplasm was clinically diagnosed as squamous cell carcinoma and was treated by surgical procedure of exeresis. To remove the tumor mass, an electric scalpel was used with a coupled steel wire and then an electric scalpel coupled with a saw wire. The animal remained hospitalized for 7 days, being medicated. After the patient's recovery, which presented excellent scar retraction of the surgical site, the patient was discharged. The surgical conduct of ECC excision is easy to perform. However, prior assessment is essential to identify the most effective treatment technique without relapse.(AU)

Carcinoma de células escamosas em dorso de bovino / Carcinoma of squamous cells in back bovine

Um boi de carroça, criado no Semi-Árido potiguar, foi atendido no HOVET-UFERSA, com aumento de volume localizado na região dorsal. A neoplasia foi diagnosticada clinicamente e confirmada após estudo histopatológico em carcinoma de células escamosas (CCE). Foi tratado por exérese cirúrgica. Para a remoção da massa tumoral utilizou-se bisturi elétrico com de fio de aço acoplado, seguida de uso de serra de Giglle. O paciente permaneceu internado durante 7 dias, sendo medicado, com cicatrização ocorrida por segunda intenção. Recuperado o paciente, com excelente retração cicatricial da ferida cirúrgica, teve alta hospitalar. A conduta de exérese cirúrgica do CCE foi rápida e de fácil execução, contudo, é fundamental a avaliação prévia, visando delinear e identificar a técnica de tratamento mais eficiente e menos invasiva.

A wagon bull, raised in the potiguar Semi-Arid, was admitted to the HOVET-UFERSA, with a swelling located on its back. This neoplasm was clinically diagnosed as squamous cell carcinoma and was treated by surgical procedure of exeresis. To remove the tumor mass, an electric scalpel was used with a coupled steel wire and then an electric scalpel coupled with a saw wire. The animal remained hospitalized for 7 days, being medicated. After the patient's recovery, which presented excellent scar retraction of the surgical site, the patient was discharged. The surgical conduct of ECC excision is easy to perform. However, prior assessment is essential to identify the most effective treatment technique without relapse.

Equine chorionic gonadotrophin improved vascularization of feline ovarian tissue xenografted into immunosuppressed mice.

The objective of this study was to evaluate effects of eCG on vascularization and development of feline ovarian tissue xenografted to immunosuppressed mice. Feline ovarian fragments (∼1 mm3) were transplanted under the renal capsule of 20 adult, ovariectomized, C57BL/6 SCID female mice. At 45 d after transplantation, 10 mice (controls) were euthanized and the remainder given 10 IU of eCG (and sacrificed 48 h later). Transplants were recovered immediately after death, fixed, sectioned, and stained with periodic acid-Schiff (PAS). Fragment volume (Cavallieri principle) and vascularization were assessed. Mean xenotransplant volume for control and treatment groups was 0.17 ±â€¯0.03 and 0.37 ±â€¯0.13 mm3, respectively (P = 0.0952); vascular volume density, 30.3 ±â€¯11.3 and 49.1 ±â€¯8.9% (P = 0.0281); surface density, 4.1 ±â€¯2.4 and 6.2 ±â€¯1.7 µm-1 (P = 0.2222); and vessel total surface, 0.63 ±â€¯0.24 µm2 and 2.28 ±â€¯1.05 µm2 (P = 0.0079). In conclusion, eCG significantly increased vascular volume density of xenotransplanted ovarian tissue and improved its development.

Valores hematológicos e bioquímicos de camundongos imunossuprimidos BALB/c NUDE E C57BL/6 SCID / Haematological and biochemical values of immunosuppressed BALB/c NUDE and C57BL/6 SCID mice

Background: The emergence of the NUDE and SCID immunosuppressed mice lineages generated knowledge on various mechanisms of lymphocyte maturation and human autoimmune diseases. Information on haematological and biochemical parameters of these lineages is still scarce, making it impossible to infer homeostasis by comparing data, or to detect genetic influences on the parameters for these species. Haematological and biochemical tests were carried out on Balb/c NUDE and C57BL/6 SCID mice of both sexes, aiming to analyse the presence of genetic influence on possible variations of such parameters and to verify reference values for both lineages.Materials, Methods & Results: One hundred and forty mice (Mus musculus) of the Balb/C NUDE and C57BL/6 SCID lineages were used in the present study. The animals were previously anesthetized, the blood collection procedure was performed by cardiac puncture and the samples were collected in the presence of heparin and intended for haematological and biochemical evaluation, under standardized conditions. The haematological evaluation consisted of red blood cell count, leukocyte counts, platelet counts, haematocrit, haemoglobin concentration, mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC). The quantified biochemical parameters were: urea, creatinine, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST) and alkaline phosphatase (ALP). While analysing the obtained data, it was possible to observe that only females presented divergences (P < 0.05) in the red blood cell series, in haemoglobin and in mean haemoglobin concentration (MCH). Regarding the analysis of the white blood cell series, females only presented differences (P < 0.05) in the leukocyte count. For males, there were variations (P < 0.05) in the counts of leukocytes, eosinophils, lymphocytes and neutrophils.[...]

Valores hematológicos e bioquímicos de camundongos imunossuprimidos BALB/c NUDE E C57BL/6 SCID / Haematological and biochemical values of immunosuppressed BALB/c NUDE and C57BL/6 SCID mice

Background: The emergence of the NUDE and SCID immunosuppressed mice lineages generated knowledge on various mechanisms of lymphocyte maturation and human autoimmune diseases. Information on haematological and biochemical parameters of these lineages is still scarce, making it impossible to infer homeostasis by comparing data, or to detect genetic influences on the parameters for these species. Haematological and biochemical tests were carried out on Balb/c NUDE and C57BL/6 SCID mice of both sexes, aiming to analyse the presence of genetic influence on possible variations of such parameters and to verify reference values for both lineages.Materials, Methods & Results: One hundred and forty mice (Mus musculus) of the Balb/C NUDE and C57BL/6 SCID lineages were used in the present study. The animals were previously anesthetized, the blood collection procedure was performed by cardiac puncture and the samples were collected in the presence of heparin and intended for haematological and biochemical evaluation, under standardized conditions. The haematological evaluation consisted of red blood cell count, leukocyte counts, platelet counts, haematocrit, haemoglobin concentration, mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC). The quantified biochemical parameters were: urea, creatinine, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST) and alkaline phosphatase (ALP). While analysing the obtained data, it was possible to observe that only females presented divergences (P < 0.05) in the red blood cell series, in haemoglobin and in mean haemoglobin concentration (MCH). Regarding the analysis of the white blood cell series, females only presented differences (P < 0.05) in the leukocyte count. For males, there were variations (P < 0.05) in the counts of leukocytes, eosinophils, lymphocytes and neutrophils.[...](AU)

Perfil hematológico e bioquímico de camundongos da linhagem Balb-c / Haematological and biochemical profile of Balb-c Mice

Background: Animal models are widely used in scientific research because of the ability to generate information from an organism like everything under a given experimental condition. Hematological and biochemical tests in laboratory animals are essential for the validation of several scientific studies. In addition, it standardizes physiological values for these animals according to their sex, age, lineage, environment, and nutritional status. The present work aims to establish reference values for biochemical and hematological standards in Balb/c mice, for males and females.Materials, Methods & Results: A total of 50 male and female mice were used at reproductive age. The procedures for collecting, processing, and analyzing the samples were standardized. The collected blood samples were immediately transferred to eppendorf tubes containing heparin, and intended for hematological and biochemical evaluation. The hematological evaluation consisted of Red blood cell count (RBC), Leukocyte counts (WBC), Platelet counts (PLT), Hematocrit (HCT), Hemoglobin concentration (HGB), Mean corpuscular volume (MCV), and Mean corpuscular hemoglobin concentration (MCHC). Already the quantified biochemical parameters were: urea, creatinine, alanina aminotransaminase (ALT), aspartato aminotransaminase (AST) and fosfatase alcalina (FAL). The differential leukocyte count was also performed. Hematological results obtained for males and females were: 9.19 ± 3.35 (106/mm³) and 7.3 ± 2.01(106/mm³) of RBC; 35.8 ± 6.7% and 38.44 ± 3.93% of HCT; 11.51 ± 2.17 g/dL and 11.85 ± 1.56 g/dL of HGB; 45.83 ± 15.03 fL and 60.26 ± 18.25 fL of VCM; 31.80 ± 1.15% and 31.88 ± 0.99% of MCHC; and, 5380 ± 1994.21(10³/mm³) and 3564 ± 1071(10³/mm³) of WBC. The platelet counts were 878.92 ± 84.19 and 678.28 ± 227.21, for males and females respectively.[...](AU)

Perfil hematológico e bioquímico de camundongos da linhagem Balb-c / Haematological and biochemical profile of Balb-c Mice

Background: Animal models are widely used in scientific research because of the ability to generate information from an organism like everything under a given experimental condition. Hematological and biochemical tests in laboratory animals are essential for the validation of several scientific studies. In addition, it standardizes physiological values for these animals according to their sex, age, lineage, environment, and nutritional status. The present work aims to establish reference values for biochemical and hematological standards in Balb/c mice, for males and females.Materials, Methods & Results: A total of 50 male and female mice were used at reproductive age. The procedures for collecting, processing, and analyzing the samples were standardized. The collected blood samples were immediately transferred to eppendorf tubes containing heparin, and intended for hematological and biochemical evaluation. The hematological evaluation consisted of Red blood cell count (RBC), Leukocyte counts (WBC), Platelet counts (PLT), Hematocrit (HCT), Hemoglobin concentration (HGB), Mean corpuscular volume (MCV), and Mean corpuscular hemoglobin concentration (MCHC). Already the quantified biochemical parameters were: urea, creatinine, alanina aminotransaminase (ALT), aspartato aminotransaminase (AST) and fosfatase alcalina (FAL). The differential leukocyte count was also performed. Hematological results obtained for males and females were: 9.19 ± 3.35 (106/mm³) and 7.3 ± 2.01(106/mm³) of RBC; 35.8 ± 6.7% and 38.44 ± 3.93% of HCT; 11.51 ± 2.17 g/dL and 11.85 ± 1.56 g/dL of HGB; 45.83 ± 15.03 fL and 60.26 ± 18.25 fL of VCM; 31.80 ± 1.15% and 31.88 ± 0.99% of MCHC; and, 5380 ± 1994.21(10³/mm³) and 3564 ± 1071(10³/mm³) of WBC. The platelet counts were 878.92 ± 84.19 and 678.28 ± 227.21, for males and females respectively.[...]

Síndrome hipereosinofílico idiopático en el perro: una descripción poco común en el noreste de Brasil / Idiopathic hypereosinophilic syndrome in dog: an unsual description in Northeastern Brazil / Síndrome hipereosinofílica idiopática em cão: uma insólita descrição no nordeste do Brasil

Síndrome hiperosinofílica idiopática (SHI) es un mieloproliferación rara en perros con presencia intensiva de los eosinófilos y los órganos de circulación. Hay pocos informes de canino SHI, y sin antecedentes de la enfermedad en el noreste de Brasil. El objetivo fue informar el SHI en un perro semiárido del noreste, haciendo hincapié en la importancia en el diagnóstico del síndrome de otras causas de eosinofilia. Un canino, macho, cuatro años mestizo, fue remitido al Hospital Veterinario de la Universidad Federal Rural de la Zona Semiárida (Mossoro, Rio Grande do Norte, Brasil). Tenía una historia de eosinofilia crónica (por anterior hematología historia). Desparasitación actualiza. Solicitada para completar el recuento sanguíneo, la citología de la médula ósea, la serología para la leishmaniasis visceral y prueba de inmunoensayo para la detección de Dirofilaria immitis. Ocurrió eosinofilia sistémica y la cantidad marcada de eosinófilos en la médula ósea. No fue positivo para las enfermedades infecciosas. La terapia se instituyó-prednisolona. Después de 15 días, hematología mostró eosinófilos en los valores de referencia, así como el recuento de eosinófilos intramedular. Después de 112 días en el tratamiento, el paciente volvió a expresar eosinofilia suero y el compartimiento intraóseo mediante la sustitución de deflazacort prednisolona. Después de 63 días del inicio de [...](AU)

Idiopathic hypereosinophilc syndrome (HES) is a rare canine mioproliferation, with high quantity of eosinophils in peripheral blood and organs. There are few case reports of this disease in dogs, and no record of the disease in the Northeast of Brazil. This paper aimed to report the HES in a semi-arid Northeast dog, emphasizing the importance in the differential diagnosis of the other causes of eosinophilia. A 4 years old mixed breed male dog was referred to the Rural Federal University of Semi-Árid Hospital (Mossoró, Rio Grande do Norte, Brazil) present cronic eosinophilia (previously hematologic test). The deworming has been done. Complete blood count, marrow bone cytology, Leishmania serological test and immunochromatographic assay for the detection of Dirofilaria immitis were requested. The laboratorial results showed systemic eosinophilia and marked amount of eosinophils in bone marrow. There was negative to infectious disease. Treatment with predinisolone was established. After 15 days, eosinophils count had returns to reference values as well as the bone marrow count. Lather than 112 days of treatment, the animal manifested serum and intraosseous compartment eosinophilia again, so prednisolona was replaced by deflazacort. After 63 days of the start this new treatment, the dog presented normal eosinophils count in blood and bone marrow. [...](AU)

A síndrome hiperosinofílica idiopática (SHI) é uma mieloproliferação rara em cães, com intensa presença de eosinófilos circulantes e em órgãos. Há poucos relatos da SHI canina, e sem registro da doença no Nordeste do Brasil. Objetivou-se relatar a SHI em um cão do semiárido nordestino brasileiro, ressaltando a importância no diagnóstico da síndrome para outras causas de eosinofilia. Um canino, macho, quatro anos, sem raça definida, foi encaminhado ao Hospital Veterinário da Universidade Federal Rural do Semi-Árido (Mossoró, Rio Grande do Norte, Brasil). Possuía histórico de eosinofilia crônica (por exames hematológicos pregressos). A vermifigução estava atualizada. Solicitaram-se hemograma completo, citologia da medula óssea, sorologia para leishmaniose visceral e ensaio imunocromatográfico para detecção de Dirofilaria immitis. Ocorria eosinofilia sistêmica e acentuada quantidade de eosinófilos na medula óssea. Não houve positividade para doenças infecciosas. Instituiu-se terapia com prednisolona. Transcorridos 15 dias, a hematologia exibiu eosinófilos nos valores de referência, assim como a contagem de eosinófilos intramedular. Após 112 dias do tratamento, o paciente retornou a manifestar eosinofilia sérica e no compartimento intraósseo, substituindo a prednisolona pelo deflazacort. Após 63 dias do início desse último, o cão possuía normalidade nos índices de eosinófilos [...](AU)

Síndrome hipereosinofílico idiopático en el perro: una descripción poco común en el noreste de Brasil / Idiopathic hypereosinophilic syndrome in dog: an unsual description in Northeastern Brazil / Síndrome hipereosinofílica idiopática em cão: uma insólita descrição no nordeste do Brasil

Síndrome hiperosinofílica idiopática (SHI) es un mieloproliferación rara en perros con presencia intensiva de los eosinófilos y los órganos de circulación. Hay pocos informes de canino SHI, y sin antecedentes de la enfermedad en el noreste de Brasil. El objetivo fue informar el SHI en un perro semiárido del noreste, haciendo hincapié en la importancia en el diagnóstico del síndrome de otras causas de eosinofilia. Un canino, macho, cuatro años mestizo, fue remitido al Hospital Veterinario de la Universidad Federal Rural de la Zona Semiárida (Mossoro, Rio Grande do Norte, Brasil). Tenía una historia de eosinofilia crónica (por anterior hematología historia). Desparasitación actualiza. Solicitada para completar el recuento sanguíneo, la citología de la médula ósea, la serología para la leishmaniasis visceral y prueba de inmunoensayo para la detección de Dirofilaria immitis. Ocurrió eosinofilia sistémica y la cantidad marcada de eosinófilos en la médula ósea. No fue positivo para las enfermedades infecciosas. La terapia se instituyó-prednisolona. Después de 15 días, hematología mostró eosinófilos en los valores de referencia, así como el recuento de eosinófilos intramedular. Después de 112 días en el tratamiento, el paciente volvió a expresar eosinofilia suero y el compartimiento intraóseo mediante la sustitución de deflazacort prednisolona. Después de 63 días del inicio de [...]

Idiopathic hypereosinophilc syndrome (HES) is a rare canine mioproliferation, with high quantity of eosinophils in peripheral blood and organs. There are few case reports of this disease in dogs, and no record of the disease in the Northeast of Brazil. This paper aimed to report the HES in a semi-arid Northeast dog, emphasizing the importance in the differential diagnosis of the other causes of eosinophilia. A 4 years old mixed breed male dog was referred to the Rural Federal University of Semi-Árid Hospital (Mossoró, Rio Grande do Norte, Brazil) present cronic eosinophilia (previously hematologic test). The deworming has been done. Complete blood count, marrow bone cytology, Leishmania serological test and immunochromatographic assay for the detection of Dirofilaria immitis were requested. The laboratorial results showed systemic eosinophilia and marked amount of eosinophils in bone marrow. There was negative to infectious disease. Treatment with predinisolone was established. After 15 days, eosinophils count had returns to reference values as well as the bone marrow count. Lather than 112 days of treatment, the animal manifested serum and intraosseous compartment eosinophilia again, so prednisolona was replaced by deflazacort. After 63 days of the start this new treatment, the dog presented normal eosinophils count in blood and bone marrow. [...]

A síndrome hiperosinofílica idiopática (SHI) é uma mieloproliferação rara em cães, com intensa presença de eosinófilos circulantes e em órgãos. Há poucos relatos da SHI canina, e sem registro da doença no Nordeste do Brasil. Objetivou-se relatar a SHI em um cão do semiárido nordestino brasileiro, ressaltando a importância no diagnóstico da síndrome para outras causas de eosinofilia. Um canino, macho, quatro anos, sem raça definida, foi encaminhado ao Hospital Veterinário da Universidade Federal Rural do Semi-Árido (Mossoró, Rio Grande do Norte, Brasil). Possuía histórico de eosinofilia crônica (por exames hematológicos pregressos). A vermifigução estava atualizada. Solicitaram-se hemograma completo, citologia da medula óssea, sorologia para leishmaniose visceral e ensaio imunocromatográfico para detecção de Dirofilaria immitis. Ocorria eosinofilia sistêmica e acentuada quantidade de eosinófilos na medula óssea. Não houve positividade para doenças infecciosas. Instituiu-se terapia com prednisolona. Transcorridos 15 dias, a hematologia exibiu eosinófilos nos valores de referência, assim como a contagem de eosinófilos intramedular. Após 112 dias do tratamento, o paciente retornou a manifestar eosinofilia sérica e no compartimento intraósseo, substituindo a prednisolona pelo deflazacort. Após 63 dias do início desse último, o cão possuía normalidade nos índices de eosinófilos [...]

Effect of different receptor sites on the development after ovarian autograft balb/c female mice / Efeito de diferentes sítios receptores no desenvolvimento do autotransplante ovariano em camundongas balb/c

The aim of this study was to compare two receptor sites in ovarian autologous transplantation in mice. Twelve Balb-c mice were randomly divided into three groups of four animals each: G1 - non-transplanted animals, G2 - animals which received the ovarian fragment in the perimetrium, G3 - animals which had the abdominal subcutaneous tissue as a receptor site. For transplantation, mice were subjected to bilateral salpingectomia. The left ovary was submitted for histological processing. The right ovarian cortex was fragmented and reimplanted in the specified places according to the groups. Transplants were recovered in 3/4 of the mice in each treatment group. In vaginal washes, the return of the ovarian activity in half of the females of G3 and 3/4 females of G2 was observed. It was detected the presence of corpora lutea in 1/4 of G3 transplants and half in G2. In follicular population all stages of development were observed. Comparing the receptor sites, it was observed a higher percentage of return to cyclicity, as well as of follicles, in animals which had the perimetrium as the site of transplantation. In the subcutaneous, there was a lower follicular growth and lower quantity of corpus luteum. Thus, ovarian autologous transplantation allocated in the subcutaneous tissue and perimetrium allowed both tissue maintenance and follicular development; however, the site of choice will depend on the final goal one aims with the technique(AU)

Objetivou-se comparar dois sítios receptores no transplante autólogo ovariano em camundongas. Doze camundongas BALB/c foram divididas aleatoriamente em três grupos experimentais com quatro animais em cada. O G1 - animais não submetidos ao transplante, G2 - animais que receberam o fragmento ovariano no perimétrio, G3 - animais que tiveram o tecido subcutâneo abdominal como sítio receptor. Para o transplante, as fêmeas foram submetidas à ovariossalpingectomia bilateral. O ovário esquerdo foi encaminhado para processamento histológico. O córtex do ovário direito foi fragmentado e reimplantado nos locais especificados de acordo com os grupos. Os transplantes foram recuperados em 3/4 das camundongas em cada grupo tratado. No lavado vaginal, observou-se em metade das fêmeas do G3 e em 3/4 das fêmeas do G2 retorno da ciclicidade. Observou-se presença de corpos lúteos em 1/4 dos transplantes do G3 e metade nos do G2. Na população folicular, foram observados todos os estádios de desenvolvimento. Comparando os sítios receptores observou-se maior porcentagem de retorno à ciclicidade, bem como de folículos, nos animais que tiveram o perimétrio como local do transplante. Já no subcutâneo houve menor crescimento folicular e menor quantidade de corpos lúteos. Dessa forma, conclui-se que o transplante autólogo ovariano alocado no tecido subcutâneo e no perimétrio permitiu tanto a manutenção tecidual quanto o desenvolvimento folicular, porém, o sítio de escolha vai depender do objetivo final que se almeja com a técnica(AU)

Morphometric alteration of intestinal epithelium of rats (Rattus norvegicus) submitted to the technique of enteropexy

Background: Due to the numerous complications that enteropexy may cause in domestic animals and humans, this study aimed at investigating the microscopic level, the damage generated by this technique in mice, in order to investigate the morphometric changes caused by enteropexy technique in rats. Materials, Methods & Results: Eighteen Wistar rats were submitted to surgical technique of enteropexy. To evaluate intestinal disorders they were euthanized at 30 (Group I), 60 (group II) and 90 (group III) days after the procedure. The animals were perfused with 2.5% glutaraldehyde and 4% paraformaldehyde for the removal of intestinal fragments for analysis in conventional microscopy and scanning electron microscopy. In classical histopathology, group I presented mild lesions with necrosis of the epithelium and infiltration of mononuclear cells. After 60 days, extensive ischemic necrosis area was observed, characterized by the denudation of epithelium cells and cellular debris in the lumen surface and loss of intestinal crypts. After 90 days (group III) lesions were evident and were characterized by strong coagulative necrosis of epithelial cells and intestinal crypts. Under electron microscopy, group I showed early fusion of the adjacent intestinal walls. After 60 days there was adherence of luminal stenosis and intestinal walls. In group III loss of epithelium and substitution [...]

Morphometric alteration of intestinal epithelium of rats (Rattus norvegicus) submitted to the technique of enteropexy

Background: Due to the numerous complications that enteropexy may cause in domestic animals and humans, this study aimed at investigating the microscopic level, the damage generated by this technique in mice, in order to investigate the morphometric changes caused by enteropexy technique in rats. Materials, Methods & Results: Eighteen Wistar rats were submitted to surgical technique of enteropexy. To evaluate intestinal disorders they were euthanized at 30 (Group I), 60 (group II) and 90 (group III) days after the procedure. The animals were perfused with 2.5% glutaraldehyde and 4% paraformaldehyde for the removal of intestinal fragments for analysis in conventional microscopy and scanning electron microscopy. In classical histopathology, group I presented mild lesions with necrosis of the epithelium and infiltration of mononuclear cells. After 60 days, extensive ischemic necrosis area was observed, characterized by the denudation of epithelium cells and cellular debris in the lumen surface and loss of intestinal crypts. After 90 days (group III) lesions were evident and were characterized by strong coagulative necrosis of epithelial cells and intestinal crypts. Under electron microscopy, group I showed early fusion of the adjacent intestinal walls. After 60 days there was adherence of luminal stenosis and intestinal walls. In group III loss of epithelium and substitution [...](AU)