ABSTRACT
Leishmania donovani-infected Syrian hamsters were treated intraperitoneally with 0.23 mmoles/kg/day of EDTA; EGTA; HEEDTA and 100 mg/kg/day of Pentostam R. The control group received 0.1 ml of phosphate buffered saline. After 30 days of treatment; the animals were sacrificed. Of the Pentostam-treated animals; 5 out 6 had negative spleen cultures; while all the chelator and PBS-treated ones yielded parasites. While all the Pentostam-treated hamsters yielded had negative bone marrow cultures; only 1 out of 6 HEEDTA-treated hamsters yielded parasites. Spleen; liver and bone marrow parasite-loads calculated from chelator-treated animals were consistently significantly higher than for Pentostam-treated animals. These results suggest that although metal ion chelators have some antileishmanial potential; their in vivo activity against L. donovani is low compared to Pentostam
Subject(s)
Animals , Chelating Agents , Leishmaniasis , Leishmaniasis/drug therapy , MesocricetusABSTRACT
The objective of this study was to undertake a dose response study to determine the optimal Pentostam and Ethylenediamine tetraacetic acid (EDTA) dose that could be used in the treatment of leishmania-infected golden hamsters or BALB/c mice for a period of 30 days. This pilot experiment was done using only one chelator; EDTA and the toxicity results obtained from this experiment formed the basis for the selection of a suitable chelator dose of this class for the future treatment of leishmania-infected laboratory animal rodent models. It is concluded that Pentostam concentrations beyond 600 mg/kg are highly toxic to mice and therefore unsuitable for use. Although Pentostam have been used to treat leishmania-infected BALB/c mice; this study has shown that a concentration of 100 mg/KG/day is the most suitable dose for use in the treatment of rodent animal models
Subject(s)
Animals , Chelation Therapy , Leishmaniasis , Leishmaniasis/drug therapyABSTRACT
Previous in vitro experiments by Mbati et al. have shown that Ethylenediamine tetraacic acid (EDTA) and Ethyleneglycol-bis (B-aminoethyl ether) N;N;N1;N1; tetraacetic acid (EGTA) substantially reduce parasite burdens of leishmania donovani in either cell free media or when engulfed in mouse peritoneal macrophages. The objective of this study was to compare the activity of the same chelators against Leishmania donovani in BALB/c mice infected with a much lower parasite inoculum
Subject(s)
Animals , Chelation Therapy , Iron Chelating Agents , Leishmaniasis , Leishmaniasis/drug therapyABSTRACT
Les poussees de fievre rhumatismale et l'endocardite infectieuse sont parmi les principales causes d'une fievre au long cours chez un cardiaque. Parfois; la non confirmation des diagnostics habituels par le bilan complementaire fait evoquer d'autres etiologies. Le contexte epidemiologique de certaines affections febriles; est; dans ce cas; essentiel dans l'orientation de discussion diagnostique. Les auteurs rapportent un cas d'association forfuite d'une valvulopathie rhumastimale et de leishmaniose viscerale chez un adulte jeune et en discutent les aspects epidemiologique; diagnostique et therapeutique