The effect of thermo-radiotherapy on multidrug resistance in tongue squamous cell carcinoma cell line Tca 8113 / 中华物理医学与康复杂志

Objective To study the effect of thermo-radiotherapy on multidrug resistance (MDR) and levels of intracellular adriamycin (ADM) in tongue squamous cell carcinoma cell line Tca 8113 and its MDR cell line Tca 8113/CBDEA. Methods Samples of the two cell lines were treated with thermo-radiotherapy (42℃ for 0.5 h and 2 Gy of radiation). Four and 24 hours later, the expression of the MDR relative proteins P-glycoprotein (P-gp),multidrug resistance associate protein 1 (MRP1) and glutathione s-tranferase-π (GST-π) were detected using immu-nohistochemistry. Intracellular ADM concentrations were measured using an HTS 7000 Plus bioassay reader. Re-sults No change in the expression of P-gp was observed in the Tca 8113/CBDEA and Tca 8113 cell lines after 4 or 24 hours. Expression of MRP1 was not significantly altered in the Tca 8113/CBDEA cell line, but there was a signifi-cant drop in the Tca 8113 cell line 24 hours post-thermo-radiotherapy. Expression of GST-π was not altered in either the Tca 8113/CBDEA or the Tca 8113 cell line at 4 hours post-thermo-radiotherapy, but there was a significant de-crease at 24 hours. At both 4 and 24 hours, drug tolerance had decreased and intracellular drug concentration had in-creased significantly in both cell lines. Conclusions Thermo-radiotherapy can enhance the effects of chemotherapy and suppress the expression of MDR factors induced by radiation. The combination of hyperthermia and radiotherapy does not induce MDR.

A biomechanic study on the relapse after sagittal split and oblique osteotomy of Ramus / 生物医学工程学杂志

The relapse after orthognathic surgery was frequently reported in recent years. But up to now researches concerning bio-mechanic mechanism of relapse are rare. The purpose of this study, by imitating the operation on monkeys, was to examine the biomechanical properties and histological characteristics after operation in order to investigate the mechanism of the relapse after orthognathic surgery. 20 rhesus monkeys divided into two groups were subjected to bilateral sagittal split and oblique osteotomy, respectively. The broken strength, stress and strain of the operated sites were examined with the use of biomechanical methods, and the healing scab was observed using histological method and SEM at 3, 6, 12, 24 weeks after operation. The results obtained demonstrated that bone healing and biomechanical properties reached certain level after 6 to 12 weeks' recovery in both operation groups; the biomechanical properties and histological characteristics recovered faster in sagittal split group than in oblique osteotomy group. Histological examination also demonstrated similar results. From the data above it is suggested that the higher relapse rate in sagittal split may more closely related to the mechanical effect different from the scab healing, although 8 weeks' fixation may meet the requirement of clinical treatment, accessory fixation method should be adopted after the removal of inter-maxilla fixation.

Clinical characters, diagnosis and treatment of parapharyngeal region neoplasms: a report of 115 cases / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study was to analyze the clinical characters, diagnosis and treatment of primary neoplasms in the parapharyngeal regions.</p><p><b>METHODS</b>A total of 115 cases of primary neoplasms in the parapharyngeal regions were included in this study.</p><p><b>RESULTS</b>Disorders of swallowing, breath or speaking were the common symptoms of neoplasms in parapharyngeal regions. The majority of neoplasms parapharyngeal regions were benign. 46 cases were from the deep lobe of the parotid, and 42 cases were neurogenic. CT or MRI provides useful information such as their sizes, shapes and extents. The relationship between the tumor and vessel was shown using super-selective angiography. All the tumors were resected completely by extra-oral approach through the posteroinferior incision of the mandible. The follow-up was carried out from 1 to 10 years, and the data indicated that 2 patients died, 18 cases were lost, and others had no recurrence.</p><p><b>CONCLUSION</b>CT and/or MRI are essentially useful for diagnosis and presurgical planning. Super-selective angiography can be used if the lesions involved carotid artery. Posteroinferior incision of the mandible is a simple, effective and safe approach for excision of neoplasms parapharyngeal regions.</p>

Induction of multidrug resistance in Tca8113 cells by transient exposure to different chemotherapeutic drugs / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to determine the effect of transient exposure to chemotherapeutic drugs on multidrug resistance of Tca8113 cells.</p><p><b>METHODS</b>The MDR1 and MRP gene expressions in Tca8113 and K562/ADM cells lines were analyzed using reverse transcription polymerase chain reaction (RT-PCR), after the cells were treated with different cytotoxic drugs. The function and expressions of P-glycoprotein 170 and multidrug resistant associated protein were studied using fluorescence photometric assays.</p><p><b>RESULTS</b>The inhibitive rate of Tca8113 cells was higher than that of K562/ADM, after exposure to chemotherapeutic drugs. The transient exposure to cytotoxic drugs weakly induced MDR1 and multidrug resistant associated protein expression in Tca8113 cells. The intracellular drug concentration in K562/ADM was lower than that in Tca8113 cells.</p><p><b>CONCLUSION</b>Induction of MDR1 and multidrug resistant associated protein gene expression response to cytotoxic drugs may be related with the increased multidrug resistance in drug-treated human tumor cells.</p>

The clinical application and evaluation of combined chemotherapy in comprehensive treatment for oral squamous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study and evaluate the clinical effects of combined preoperative chemotherapy and their relations with multi drug resistance (MDR).</p><p><b>METHODS</b>102 cases with oral squamous cell carcinoma(OSCC) were included in the study (63 males and 39 females, aged 22 to 67 years). Among the subjects there were 57 cases with cancer of tongue and 45 cases with cancer of buccal mucosa. 27 cases in the group were classified as stage II, 55 as stage III and 20 cases as stage IV according to TNM standard. All cases accepted PYM + 5-Fu + DDP combined chemotherapy pre-operatively. The total given dose was PYM 48 mg, 5-Fu 7.5 g and DDP 300 mg. After the chemotherapy, radical surgery were performed within 2 weeks. The diagnosis of all cases were proved as OSCC by biopsy.</p><p><b>RESULTS</b>Total effective rate of the combined chemotherapy was 82.4%. All of the cases were followed up and their 3 years' survival rate was 67.6%.</p><p><b>CONCLUSION</b>The combined chemotherapy of PYM + 5-Fu + DDP is effective in using as one of comprehensive treatment for OSCC.</p>

Reversal effect of hyperthemia on multidrug resistant phenomena / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was investigate the effect of hyperthemia on multidrug resistance in K562/ADM cell.</p><p><b>METHODS</b>The MDR1 (mulitdrug resistance gene) and MRP (multidrug resistant associated gene) gene expressions in Tca8113 and K562/ADM cell lines were analyzed by RT-PCR after treated with different cytotoxic drugs and different temperature (37 degrees C and 41 degrees C). The function and expression of Pgp and MRP were detected by fluorescence photometeric assays.</p><p><b>RESULTS</b>Inhibition rate of both cells was significantly enhanced by exposure to chemotherapeutic drugs and 41 degrees C temperature; Exposing to 41 degrees C hyperthemia reduced MDR1 and MRP expression and enhanced intracellular drug concentration as well in K562/ADM.</p><p><b>CONCLUSION</b>41 degrees C hyperthemia could effectively enhance the inhibition rate of chemotherapeutic drugs and partially reverse the multidrug resistance. It is suggested that hyperthemia could be used as a method to overcome multidrug resistance.</p>

Regulation of XW630 on estrogen receptor messenger RNA expression in osteoblasts / 生物医学工程学杂志

This study was performed to investigate the mechanism of an anti-osteoporosis new drug XW630 for promoting the osteogenic action. Osteoblast clone was cultivated from SD adult rat calvaria in vitro, the estrogen receptor messenger RNA(ERmRNA) expression in the osteoblasts of rats was detected directly with high-sensitive RT-PCR firstly. The results indicated that XW630 can significantly promote ERmRNA expression in osteoblasts in the time-dependent manner. Being superior to other two kinds of estrogen in the same concentration (10(-6) mol/L), XW630 probably plays an important role in the bone pathogenesis by means of ER gene regulatory functions.

Distraction osteogenesis for repair of cleft palate: an experimental study / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To observe the repair of cleft palate (CP) bone defect under the application of distraction osteogenesis (DO); to investigate the correction mechanism in thin bone of palatal shelf and to search a possible new treatment approach for repair of CP.</p><p><b>METHODS</b>14 cats were assigned randomly to 3 groups: (1) Empty control, 2 cats unoperated with euthanasia at the end of 2 weeks; (2) Experimental control, 2 cats underwent 8 mm x 18 mm palatal osteoectomy in saggital direction as the experimental model of CP with euthanasia in 6 weeks; (3) Experimental group, 10 cats were performed the same palatal osteoectomy as group (2), the newly designed and individual manufactured intraoral distractors were fixed to teeth by brackets and to bilateral palate bone across the defect with titanium miniscrews. 4 weeks later the transport disc (TD) osteotomy were performed and gradual distraction was started after 6 days latency period at the rate of 0.4 mm x 2/day till the TD reached the opposite edge of the CP. Roentgenographic, gross meterage and ultrastructural observation were performed 2, 4, 6, 8, 12 weeks after completion of distraction.</p><p><b>RESULTS</b>All the examinations revealed the TD recombination to the opposite bone edge of the CP, simultaneously, the proportional expansion of overlay mucoperiosteal flap was achieved. The bone defect were filled with de move osteogenesis. Roentgenographically, the new bone mineralization was along the DO vector, developed from the bone cut edges bilaterally to the central translucent zone until turned to thin fissure. Comparison of the plaster casts showed no change in the occlusion or dentomaxillofacial framework.</p><p><b>CONCLUSIONS</b>The intraoral distractor is able to get both effective distraction and steady fixation. The examination results suggest that the CP bone and soft tissue defect could be repaired and acheived functional reconstruction under DO, which could be a new treatment approach for repair of CP.</p>

Study of XW630 in promoting estrogen receptor expression in ovariectomized rats / 生物医学工程学杂志

We adopted firstly the dextran-coated charcoal(DCC) and SP methods to detect estrogen receptor (ER) expression of bone tissue in ovariectomized(OVX) rats. The results demonstrate that in OVX rats, XW630 can significantly promote ER expression in bone tissue and increase the ER content. XW630 is superior to estrone in effectiveness. The results also reveal that the ER expression in OVX rat bone tissue decreases with the lapse of time, indicating that the expression of ER depends on the existence of estrogen.

Effects of epidermal growth factors on the proliferation and metabolism of A/J mouse embryonic palatal cells / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to investigate the effects of epidermal growth factors (EGFs) with different concentration on the OD, DNA, protein, and PGE2 of A/J mouse embryonic palatal shelves cells (A/J MEPC) isolated from embryonic palatal shelves.</p><p><b>METHODS</b>The mouse embryonic palatal shelves cells were grown in different 39 pores (or bottles) with 9 gradient concentrations of EGF (0.005, 0.010, 0.050, 0.100, 0.500, 1.000, 5.000, 10.000, 50.000 ng/ml), and four pores were prepared for the same concentration, then the OD, DNA, protein and PGE2 of A/J MEPC were measured after 1 day, 3 days and 5 days.</p><p><b>RESULTS</b>EGFs stimulated DNA and PGE2 synthesis of A/J MEPC, and augmented proliferation index (PIX). Their effects were very obvious in promoting the proliferation of A/J MEPC, when the concentration was 10.000 ng/ml.</p><p><b>CONCLUSION</b>EGF may be important in regulating proliferation and metabolism of embryonic palatal shelves cells.</p>

Distraction osteogenesis for the repair of cleft palate--an ultrastructural study / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study was to explore ultrastructural characters of the newly formed bone in the correction of cleft palate (CP) bone defect by distraction osteogenesis (DO).</p><p><b>METHODS</b>The CP experimental animal models (12 cats) were established surgically, and were divided randomly into the experimental group (10 cats), in which the hard palate bone defects were corrected with DO procedure at the rate of 0.4 mm x 2/day. The specimen retrieval with euthanasia was carried out at 2, 4, 6, 8, 12 weeks after completion of distraction. Ultrastructural study was then performed; the experimental control group (2 cats) was kept for 6 weeks before euthanasia without any correction, the other extra 2 cats were used as the negative control.</p><p><b>RESULTS</b>New bone formation appeared in early 2 weeks. Exclusively intramembranous bone formation was observed in all specimens. The remodeling activities were keep observed throughout the period of study, and the bone structure matured gradually till 12 weeks after the completion of DO. No repair was observed in experimental control group.</p><p><b>CONCLUSION</b>The reconstruction of CP bone defect by means of DO could get active intramembranous bone formation and remodeling, which adapted to normal functional activities.</p>

Characteristics of BMP expression and X-ray films in distraction osteogenesis for repair of cleft palate--an immunohistochemical and roentgenographic study / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to observe the roentgenographic characters of distraction osteogenesis (DO) correction of cleft palate (CP), to study the expression of BMP proportional to fixation period time intervals, and to explore the new bone formation mechanism in Cleft Palate bone shelf.</p><p><b>METHODS</b>12 cats were used to establish the CP animal model surgically, and then were assigned randomly to (1) Experimental group (12 cats): CP defects were DO repaired at the rate and rhythm of 0.4 mm x 2/day. Specimen retrieval at 2, 4, 6, 8, 12 weeks after completion of distraction, roentgenography and Anti-BMP immunohistochemistry studies were performed; (2) Experimental control group (2 cats): CP defects without any treatment procedures, and (3) Empty control group of 2 cats.</p><p><b>RESULTS</b>Anti-BMP immunohistochemistry study showed positive DAB dye in early 2 weeks and most extensively positive expression of BMP in 4 to 6 weeks. The expression of BMP wore off gradually through 8 to 12 weeks. The roentgenography showed that the newly mineralized bone was developed from the cut bone edges bilaterally to the central transparent zone, and the newly formed bone bridged the defect area completely at the end of the study. The CP bone defect was reconstructed and the distraction gap was filled with de nove osteogenesis. No new bone formation was observed in experimental control group.</p><p><b>CONCLUSION</b>The process of new bone formation in the distraction area is dynamic. Being stimulated primarily, the process was kept highly active till quiescence phase finally. The X-ray examination shows that there is distinctively low roentgenopeque. Nevertheless, roentgenography is so far a very effective and convenient method to evaluate and monitor the DO correction efficiency.</p>

Biomechanical properties of regenerated bone by mandibular distraction osteogenesis / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To study the biomechanical properties of the new bone generated by mandibular distraction osteogenesis (DO).</p><p><b>METHODS</b>A total of 11 healthy adult goats were randomly divided into 2 groups, the experimental group (n=9) and the control group (n=2). For the goats in the experimental group, the bilateral mandibles were gradually lengthened for 10 mm with distraction appliances. Three goats were sacrificed respectively at 2, 4 and 8 weeks after completion of distraction. Compressive, three-point bending and shearing tests were conducted on the standard regenerated bone samples and the whole unilateral mandibular specimens. For the goats in the cont rol group, no operation was made and the whole unilateral mandible was taken as the test specimen.</p><p><b>RESULTS</b>The compressive strength and bending stiffness of the new bone reached the normal level at 4 and 8 weeks after completion of distraction, respectively. But the shearing strength remained significantly weaker than that of the controls at 8 weeks after distraction.</p><p><b>CONCLUSIONS</b>The distraction appliance can be removed and the lengthened mandible should be exposed to adaptive functional exercise at 8 weeks after completion of distraction.</p>

Spatial and temporal features of proliferating vessels during mandibular distraction osteogenesis / 实用口腔医学杂志

Objective:To study the spatial and temporal features of p roliferating vessels during mandibular distraction osteogenesis. Methods :Bilateral mandibles of twelve adult goats were lengthened by 10 mm wit h a custom-made distractor at 1 mm/d, two animals were killed at each intervals . Two additional goats were used as normal control. SEM microvascular corrosion casting was used to examine the spatial and temporal features of proliferative v essels within distraction gap. Results:During distraction perio d, numerous vessels from bone marrow and periosteum were regenerated into distra ction gap and began to form vascular network. In the early phase of fixation per iod, the orientation of vein sinus was in accord with distraction orientation. A long with the time, more vessles were connected, regeneration of vessels vanishe d, and the vascular system within distraction gap became more mature.Con clusion:Angiogenesis during DO is closely related to the osteogenesis; the blood supply of new bone in distraction gap is from both bone marrow and per iosteum.

Experimental study on microcosmic changes of compressed premaxillary suture / 实用口腔医学杂志

Objective: To study the microcosmic and pathological changes of compressed premaxillary suture in rabbit models with the cleft in premaxilla.Method: 12 male New Zealand rabbits, at the age of 3 weeks were designed randomly into 3 groups with 4 in each. In group 1and 2 a retropress force of 2.24 and 1.12 N was applied on the maxilla of the animals respectively. The other group was the control. The experimental animals were killed and compressed premaxilla suture specimens were examined by microscopy and electron microscopy 3,6,9 and 12 weeks after operation respectively. Result:In group 1 disturbance of microstructure and degeneration of osteoblasts were observed 3 and 6 weeks after application of the compress force. In control group normal bone structure and development of maxilla were observed. The microcosmic changes in group 2 were similar to those in group 1, but slighter. The microcostructure in the compressed premaxilla tended to be normal 9 weeks after operation. Conclusion:Compress force on premaxillary suture may result in limited pathological changes of the bone during early developing stage.

Analysis of gene expression profiles in Tca8113/CBP nude mice by microarray technique / 实用口腔医学杂志

Objective:To study the differences of gene expression between Tca8113 and Tca8113/CBP tissues in nude mice. Methods:Tca8113 cells were injected subcutaneously in both sides of armpits of nude mice at the concentration of 5?106 cells/0.1 ml. Two weeks after injection, Carboplatin was used subcutaneously around the tumor 0.01 mg/g (weight) each day in Tca8113/CBP group while Tca8113 group was injected with physiologic saline as control. Mice were sacrificed 10 weeks after drug injection. The two kinds of tissues were investigated by human 16k cDNA v2.1 SBC-R-HC-100-21 gene chip. Results:Among the 16 000 target genes, there were 719 genes whose expression levels showed differences between the two kinds of tumor tissues. Conclusion:Microarray technique can simultaneously screen different genes from above-mentioned two kinds of tissues. Further analysis of the obtained genes will be helpful to understand the molecular mechanism of multidrug resistance.

Effect of radiation on multidrug resistance of tongue squamous cell carcinoma cell line Tca 8113 / 实用口腔医学杂志

Objective:To study radiation sensibility of tongue squamous cell carcinoma cell line Tca 8113 and its multidrug resistance(MDR)cell line Tca 8113/CBDEA.Methers:MTT assay was used to determine the sensitivity of the cells to radiation.Cells were treated with 2 Gy of ?-radiation for 4 or 24 h.Real-time quantitative reverse transcriptase-polymerase chain reaction(RT-PCR)was used to detecte the expressions of mdr related genes(mdr1,mrp1,gst-?)and HTS 7000 Plus Bio Assay Reader was used to measure intracellular ADM concentration.Results:Tca 8113/CBDEA cells were 1.24-fold more radiation resistant than that of Tca 8113 cells(P0.05),while increased 24 h after radiation(P

Treatment of orbital floor fracture with orbital floor mesh plate / 实用口腔医学杂志

Objective:To evaluate the effect of orbital floor mesh plate in the treatment of orbital floor fracture. Methods:27 patients with orbital margin fractures were treated by surgical operation with orbital floor mesh plates or medial wall plates. All patients were observed for 3 months after operation.Results:It was observed that all patients with enophthalmos were cured and the patients with nerve injury recovered satisfactorily.Conclusion:Orbital floor mesh plate is effective in the treatment of orbital floor fracture.

The effect of heat shock on the expression of urokinase-type plasminogen activator receptor in human tongue squamous cell carcinoma Tca 8113 cells / 实用口腔医学杂志

Objective: To observe the expression of urokinase-type plasminogen activator receptor (uPAR) in human tongue squamous cell carcinoma Tca 8113 cells at different dosages of heat shock. Methods:The expression of uPAR protein in Tca 8113 cells was examined using immunohistochemical technique (IH) and flow cytometry (FCM) after the cells had been treated at 37,40,43 or 45 ℃ for 40 min. Results:The fluorescent intensity of uPAR protein of Tca 8113 cells treated at 37,40,43 and 45 ℃ was 11.36?0.06,9.98?0.12, 10.37?0.05 and 11.31?0.10 respectively.Conclusion:Heat shock can reduce the expression of the uPAR protein of Tca 8113 cells.

TNP-40 inhibits the growth of submandibular sarcoma in KM mice / 实用口腔医学杂志

Objective: To study the effect of a potent angiogenic in hi bitor TNP-470 on the growth of sarcoma. Methods:1?10 6 sarcom a S-180 cells 0.1 ml were inoculated into submandibular region in each of 40 K M mice. The mice were divided into control and treatment groups with 10 in each group. Treatment was started 8 hours after inoculation. TNP-40 at 10 mg/kg, 30 mg/kg and 100 mg/kg was given subcutaneously every other day in the 3 treatment groups,total 6 times. On the 12th day, the mice were sacrificed, tumor and mice were weighted. Apoptosis of tumor cells was observed by TUNEL method and transmi ssion microscope. Express of VEGF, bFGF were detected by immunohistochemical sta ining. Results:Sarcoma was developed in all of the mice. The sa rcoma cells invaded deep into adjacent organs and tissues such as muscle, subman dibular gland, parotid and facial nerve. 10 mg/kg, 30 mg/kg and 100 mg/kg TNP- 470 inhibited the growth of the tumor by 27.62%,63.81% and 85.71% respective ly, increased the apoptosis cell number by 54.46%,156.69% and 432.48% respect ively (P