Clinicopathologic Implication of Ezrin Expression in Non-small Cell Lung Cancer

BACKGROUND: Ezrin, a member of the ezrin-radixin-moesin family, is implicated in tumor progression, metastatic dissemination, and adverse outcomes, in several cancer types. In this study, we explored the clinicopathological significance of ezrin expression in non-small cell lung carcinomas (NSCLCs). METHODS: Immunohistochemical analysis of tissue microarray with 112 surgically resected NSCLC specimens, was performed to examine the ezrin expression. We also correlated ezrin expression with other clinicopathological features and prognosis. RESULTS: The ezrin-positive group revealed significantly higher correlation with pleural invasion (p=0.016) and pathologic stage (p=0.050). Univariate survival analysis showed that ezrin-positive group had a significantly shorter cancer-specific survival than ezrin-negative group (p=0.016). Meanwhile, female (p=0.030), no pleural invasion (p=0.023), no lymphatic invasion (p=0.026), and early pathologic stage (p=0.008) significantly correlated with longer survival. Multivariate survival analysis showed that variables such as ezrin positivity (p=0.032), female (p=0.035), and early pathologic stage (p=0.001) were independent prognostic factors for NSCLC. CONCLUSIONS: Ezrin might be a molecular marker to predict poor prognosis of NSCLC.

Angiolipoma of the Breast: A Case Report / 한국유방암학회지

An angiolipoma is a common mixed connective tissue tumor that commonly occurs in the trunk and extremities, and has been rarely reported in the breast. It is a benign variant of a lipoma that shows various appearances on mammography. It is usually seen with a well circumscribed homogeneously hyperechoic pattern on ultrasound. We report a rare case of an angiolipoma of the breast with no definite abnormality on mammography, appearing as an irregular homogeneously hyperechoic lesion on ultrasound.

Expression of HER-2/neu and Paxillin in Ductal Carcinoma in situ, Invasive Ductal Carcinoma with Ductal Carcinoma in situ and Mucinous Carcinoma / 한국유방암학회지

PURPOSE: We purpose to determine the correlation of HER-2/neu and paxillin expression in ductal carcinoma in situ (DCIS), invasive ductal carcinoma with ductal carcinoma in situ (IDC with DCIS) and mucinous carcinoma. METHODS: To evaluate the expression of HER-2/neu and paxillin, the immunohistochemical staining was performed for 13 cases of DCIS, 13 cases of IDC with DCIS and 6 cases of mucinous carcinoma. RESULTS: The DCIS and IDC were associated with infiltration of the inflammatory cells, especially in the comedo type and solid type of tumor. In cases with infiltration of the inflammatory cells, HER-2/neu and paxillin were strongly expressed. When comparing the expression level of HER-2/neu from adjacent normal tissue between DCIS and IDC with DCIS, expression of HER-2/neu was similar to that of normal tissue adjacent to DCIS. However, in the adjoining normal ductal epithelial cells, paxillin was highly expressed in cells of all of the tumor types, and especially for IDC with DCIS. HER-2/neu and paxillin were not expressed in mucinous carcinoma cells in all cases. CONCLUSION: HER-2/neu in the DCIS and IDC with infiltration of inflammatory cells shows higher expression than non-inflammatory DCIS and IDC. If normal duct epithelial cells show a high level of HER-2/neu expression, the epithelial cells have a high probability of transformation into anaplastic cells. However, paxillin appears to have no value as a prognostic factor. The difference of expression of HER-2/neu between IDC with DCIS and DCIS suggests a different origin of tumor cells. The growth pattern of mucinous carcinoma cell is different from the that of DCIS or IDC cell, which grow slowly.

Prognostic Value of Apoptosis and the Survivin, bcl-2, and p53 Expressions in Breast Cancer Patients / 한국유방암학회지

PURPOSE: Survivin is a member of the inhibitor of apoptosis (IAP) protein family, and it is involved in the regulation of cell division. The over-expression of survivin has been reported to be associated with the parameters for a poor prognosis in most human cancers, including lung, breast, colon, stomach, esophagus, pancreas, etc. In this study, we examined the expression of a member of a novel IAP protein family, survivin, in breast cancer and its association with tumor cell apoptosis and the overall prognosis. METHODS: 80 cases of formalin-fixed paraffin-embedded breast cancer tissue were immunostained with, using polyclonal survivin (Novus Biologicals, Littleton, USA), monoclonal bcl-2 (DAKO, Carpinteria, USA), and monoclonal p53 antibodies (DAKO, Carpinteria, USA). The histochemical method used for the analysis of apoptosis was based on ApopTag. Peroxidase In Situ OligoLigation (ISOL) Apoptosis Detection Kit (CHEMICON International Inc. Temecula, USA). RESULTS: Immunohistochemical analysis showed that cytoplasmic survivin expression was positive in 43 of 80 cases (53.8%) of breast carcinomas and it was positive for 70% of the cases that showed a bcl-2 expression tumors. Statistical analysis revealed that the survivin expression was correlated with lymph node metastasis, the tumor stage, and the histological grade. Although the survivin expression was not correlated with p53 mutations, the survivin positive cases were associated with a bcl-2 expression (p=0.015) and a reduced apoptotic index (p=0.024). On the Cox proportional hazard model analysis, the apoptotic index was not identified as a significant independent predictor of overall survival (p=0.072), although the patients with a low apoptotic index ( or =0.2%). CONCLUSION: The results suggest that apoptosis inhibition of apoptosis by survivin may be a prognostic parameter for a worse outcome in breast carcinoma patients.

The Effects of Hyperosmolar Dextrose and Autologous Serum Injection in the Experimental Articular Defect of Rabbit

OBJECTIVE: Although the clinical effects of prolotherapy on osteoarthritis has been reported, there have been few previous studies showing the effects as a proliferant on articular cartilage. Also the autologous blood has been reported to used as a growth factor stimulant recently, we were trying to use dextrose and autologous serum for tissue regeneration respectively and evaluated the proliferative effect of autologous serum comparing with that of dextrose. METHOD: Twenty four rabbits were used for this study. The rabbits were divided into three groups. Group A did not get any special treatment. Group B was treated with 10% dextrose and group C with autologous serum. Six weeks later, gross appearance and histologic findings were evaluated. RESULTS: After sacrifice, the gross inspection of the knee joints revealed that group B and C were filled with the translucent tissue in defective cartilage. Group A still had defective cartilage. Histologic evaluation revealed increase of cellularity in the defect of the injected specimens when compared with the control. There was no morphological difference between group B and C. CONCLUSION: The repair process of the articular cartilage defects using dextrose and autologous serum were shown to be more effective than that of control group.

A Case of Acute Generalized Exanthematous Pustulosis / 소아알레르기및호흡기학회지

Acute generalized exanthematous pustulosis(AGEP) is a relatively rare exfoliative skin syndrome. It consists of a generalized eruption of pustules in response to medication or infection. Antibiotics are the most commonly implicated medications. Nonsteroidal anti-inflammatory drugs(NSAID) can also trigger AGEP. However, So far, a case of AGEP due to ibuprofen has not been reported in Korea. Therefore we report a case of acute generalized exanthematous pustulosis as a result of use of ibuprofen in a 10 years old female.

A Case of Periumbilical Perforating Pseudoxanthoma Elasticum / 대한피부과학회지

Perforating pseudoxanthoma elasticum is a rare disorder. It is an hereditary connective tissue disease involving progressive fragmentation and dystrophic calcification of elastic fibers. The localized lesions usually occur in a periumbilical location in obese, middle-aged, multiparous black women. It is characterized clinically by yellowish, lax, well-circumscribed, reticulated or cobblestoned patches or plaques of the periumbilical region. Herein, we report a case of periumbilical perforating pseudoxanthoma elasticum which occurred in an obese, multiparous woman with no signs of hereditary systemic pseudoxanthoma elasticum.

The Response of the Injured Articular Cartilage of Rabbit Knee after Injection of Autologous Blood

OBJECTIVE: To evaluate the effects of proliferant by injecting blood into the articular cartilage defect. METHOD: The patella of rabbits were dislocated laterally and 2 mm circular and 2 mm depth full-thickness defect was made in the articular cartilage. We injected 0.2 cc autologous blood to the right defect and normal saline to the left one at 1 week after operation for six times with a 1 week interval. After injection for six weeks, the articular cartilage defect were obtained and stained with H-E and S-100. RESULTS: The surface of the saline-injected group was easily distinguishable from the surrounding articular cartilage. But the blood-injected group had similar appearance to the surrounding cartilage, with the margin of the defect barely discerptible. Strong S-100 stained immune cartilage cells were observed in the blood-injected group. CONCLUSION: The repairing process of the injured articular cartilage using autologous blood was shown to be much better than that of saline-injected group although the observation period was short and the number of animal was small. So we found that autologous blood effectively repaired osteochondral defects.

Expression of Survivin in Patients with Breast Cancer / 한국유방암학회지

PURPOSE: Survivin is a member of the inhibitor of apoptosis (IAP) family, which is also involved in the regulation of cell division and is also overexpressed and associated with parameters of poor prognosis in most human cancers, including carcinomas of the lung, breast, colon, stomach, esophagus and pancreas. This study examined the expression patterns of survivin in normal breast tissue, atypical hyperplasia, primary breast cancer and lymph node tissues involved with breast cancer and to determined whether the expression of survivin is associated with the characteristics and prognosis of breast cancer. METHODS: Formalin-fixed paraffin-embedded samples from 80 breast cancer, 20 atypical hyperplasia and 20 malignant lymph node tissue cases were immunostained using polyclonal survivin (Novus Biologicals, CO). The degree of immunostaining was recorded on a scale of 0-3 according to the percentages of staining and distributions within the cytoplasm & nucleus. RESULTS: Survivin was expressed in 52, 14 and 17 of the 80 breast cancer (65%), atypical hyperplasia (70%) and breast cancer lymphoid (85%) specimens respectively. Among those expressing cancer, 11.3, 31.3 and 22.5% demonstrated nuclear staining only, cytoplasmic staining only and both nuclear and cytoplasmic staining respectively. A statistical analysis revealed that cytoplasmic survivin expression was correlated with the stage, histological grade and L/N metastasis. In a Cox proportional hazard model analysis, the expression of survivin was not identified as a significant independent predictor of overall survival (P=0.168), although the decrease in the survival rate of survivin-positive patients did reach statistical significance (P=0.048). CONCLUSION: Our results show that survivin is frequently overexpressed in primary breast cancer and its expression gradually increased from normal breast tissue to malignant lymph nodes. The expression of cytoplasmic survivin was common in breast cancer and could be both a useful diagnostic marker and important source of prognostic information.

Clinical Correlation of HER-2/neu Overexpression in Patients with Breast Cancer / 한국유방암학회지

PURPOSE: Breast cancer is a common malignant tumor for Korean women. Various oncogenes have been demonstrated in malignant tumor cells. There were many reports on the relationship between the prognosis and the tumor markers, and particularly for HER-2/neu; however, this relationship is controversial. The aim of this study was to perform immunohistochemical staining for the HER-2/neu antigenic protein in breast cancer patients to evaluate the expression rate of this antigenic protein, and to compare the correlations with the patients' prognosis. METHODS: The medical records of 100 patients who were diagnosed with breast cancer and who were treated with mastectomy between January 1996 and December 1999 at Soonchunhyang University Cheonan Hospital were selected according to the tissue condition of the paraffin block fixations. The prognostic factors were then investigated. The immunohistochemical expression of HER-2/neu was examined and compared with the survival rate using a Kaplan-Meier estimate and a log rank test. RESULTS: One hundred patients were included in this study, had a mean (SD) age of 47.9 (plus or minus 10.5) years and a follow-up duration of 59.9 (plus or minus 13.3) moths The overall mortality was 35% (35/100) and the mean survival time (SD) was 64.4 (plus or minus 12.0) months. The overall 5-year survival rate was 77.0%. Among the study variables, the tumor stage was a significant predictor of survival, and it as associate with a significantly low survival rate for stage III tumor. Lymph node metastasis, hormonal receptor status, histologic grade and HER-2/neu overexpression were significantly associated with the survival rate for patients with breast cancer. CONCLUSION: HER-2/neu is a very useful prognostic factor, and statistical significance was achieved for this factor.

A Case of Malignant Melanoma Presenting as a Breast Mass / 한국유방암학회지

Malignant melanomas arising in the skin, buccal mucous membranes. and retina are encountered frequently. A malignant melanoma characteristically disseminates widely but infrequently metastasizes to the breast, and primary melanoma of the breast is even rarer. Primary melanomas can arise in the glandular tissue of the breast. We report a case of malignant melanoma either primary in the breast or metastatic from an unknown primary that presented initially as a left breast mass without a detectable cutaneous lesion.

Comparison of Immunohistochemical Staining of bcl-2 and PCNA in Keratoacanthoma and Squamous Cell Carcinoma / 대한피부과학회지

BACKGROUND: The function of the bcl-2 oncogene was known to prolong cell life by inhibiting apoptosis. PCNA have been used as a cellular proliferation marker. Because there are much similarities between Keratoacanthoma(KA) and Squamous Cell Carcinoma(SCC), it is often hard to differentiate KA and SCC, clinically and histopathologically. Although a number of recent studies have been attempted to separate these two entities by immunohistochemical stains, the distinction between KA and SCC may be still debatable and a matter of speculation. OBJECTIVE: The purpose of this study was to evaluate the usefullness of the method by analyzing immunohistochemical expression of bcl-2 and PCNA in a matter of differentiating KA and SCC. METHOD: 11 cases of Keratoacanthoma and 22 cases of Squamous Cell Carcinoma which are conformed by histopathologic examination were stained with bcl-2 and PCNA immunohistochemically. RESULTS: 1. In KA, The bcl-2 was negative in 100% and the PCNA was positive in 100%. 2. In SCC, The bcl-2 was negative in 95% and the PCNA was positive in 100%. 3. In the pattern of staining of PCNA, KA have more peripheral pattern(73%) than diffuse pattern(27%), otherwise SCC have more diffuse pattern(59%) than peripheral(27%) and focal pattern(14%). CONCLUSION: bcl-2, PCNA, Keratoacanthoma(KA), Squamous Cell Carcinoma(SCC)Our research showed that KA is almost similar to SCC except that there are difference in the pattern of staining of PCNA. According to the result of our study, we think that it is hard to differentiate between KA and SCC only by immunohistochemical staining of PCNA. Althogh immunohistochemical staining of PCNA would not be a confirmative method, it will help us to distinguish KA from SCC as a supplementary measure. By the fact, more researches are necessary to differentiate KA and SCC by immunohistochemical staining.

The Expression of p53 and p21 in Intradermal Nevus, Junctional Nevus and Malignant Melanoma / 대한피부과학회지

BACKGROUND: The function of the p53 protein is known to regulate cell proliferation by inhibiting cells entering S phase, so DNA damaged cell proliferation is inhibited by apoptosis. p21 is a cyclin dependent kinase inhibitor induced by wild type p53, not mutant p53. Thus p21 is thought to mediate the signal of p53 induced by DNA damaged agents to arrest the cell cycle in G1 phase. p53 and p21 are expressed in many malignant tumors, and its role in oncogenesis, tumor progression and prognosis are important. OBJECTIVE: The purpose of this study was to analyze immunohistochemical expression of mutant p53 and p21 protein in melanocytic lesions. METHOD: 11 cases of intradermal nevus, 7 cases of junctional nevus and 6 cases of malignant melanoma were immunohistochemically stained with p53 and p21 monoclonal antibodies. RESULTS: 1. In intradermal nevus, the p53 was negative in 100% and the p21 was negative in 98%. These findings suggest that the composing cells of intradermal nevus is completely mature cell. 2. The positive rates of p53 and p21 in junctional nevus were 43% and 43%, respectively. The positive rates of p53 and p21 in malignant melanoma were 82% and 67%, respectively. CONCLUSION: If the expression of p21 is induced by p53 independent pathway, the cell cycle can be arrested in G1 phase, so the tumor cell proliferation is inhibited. But if the expressed p21 is mutated as p53, it means that the natural function of p21 disappears. More research is necessary about the nature of p21 which is expressed with mutant p53.

Age Related Morphological Study on the Spleen of Mouse / 대한해부학회지

In this study, the structural components of mouse spleen were compared during their aging processes. Splenic tissues of 1 week-, 5 weeks-, 8 weeks-, 6 months-, 12 months-, 18 months-, 24 months-, and 30 months-old ICR mice were dissected out under anesthesia. Pieces of the tissues were taken from the spleen, fixed in 10% neutral formalin for light microscopy, and some splenic tissues, were prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde followed by post-fixation with 1% osmium tetroxide for electron microscopy. Paraffin sections stained with hematoxylin-eosin, Masson's trichrome, Bielschowsky's silver impregnation or aldehyde-fuchsin, were observed with light microscope. Ultrathin sections stained with uranyl acetate and lead citrate were observed with a JEM 100CX-II electron microscope. The observed results were as follow: 1. The thickness of the splenic capsule was prominently increased between 1 week-old and 5 weeks-old ones, whereas after 5 weeks-old, the thickness was very slightly increased during aging. 2. In the 1 week-old, 5 weeks-old and 8 weeks-old mice, blood forming cells were observed more frequently than those found in older ones. 3. The collagenous fibers and elastic fibers were increased in the spleens of 12 months-old mice, whereas after 18 months-old, fibers were not increased during aging. 4. The reticular fibers were increased by 8 weeks, whereas fibers were not increased afterwards. 5. In the 1-week old, mast cells were observed frequently, whereas from 5 weeks to 6 months they were observed rarely, and after 12 months, mast cells were observed frequently. 6. In the 5-weeks old, distended cisternae of granular endoplasmic reticula were first observed within the plasma cells. 7. After 12 months, the mast cell containing phagocytosed debris were observed frequently. 8. After 18 months, the plasma cell containing irregular distended cisternae of granular endoplasmic reticula were observed frequently. 9. In 30 months, the plasma cells containing myelin figures were observed frequently. From the above results, it was suggested that spleen of the mouse matures structurally in five weeks, but the function of the spleen is suppressed around 18 months, and thereafter the functional suppression is continued on aging.

Effects of Antitumor Agents on the Spleen of Mouse Implanted with Ehrlich Carcinoma Cells: An Autoradiographic Study / 대한해부학회지

In this experiment, side effects of three anticancer drugs (5-fluorouracil, mitomycin C and AG60) on the structure of spleen were histologically studied. Each of ICR mice was inoculated with 1X10(7) Ehrlich carcinoma cells subcutaneously in the inguinal area. From next day, 0.2 ml of saline solution, 5-fluorouracil (30 mg/kg), mitomycin C (400 micorgram/kg), 5 mg/kg of AG60, or 30 mg/kg of AG60 (acriflavine (1) : guanosine (1) composition, Taerim Pharm. Co., Seoul, Korea) were injected subcutaneously every other day. The day following the 7th injection of anticancer drugs, each mouse was injected with a single dose of 0.7 microCi/gm of methyl-(3)H-thymidine (25 Ci/mmol, Amersham Lab., England) through tail vein. Seventy minutes after the thymidine injection, animals were sacrificed, and splenic tissues were collected and fixed in 10% neutral formalin. Tissue blocks were washed, dehydrated, embedded and cut into 6 micrometer-thick sections. Deparaffinized sections were coated with autoradiographic emulsion EM-1 (Amersham Lab., England) in the dark room and dried, and were kept in a light-tight box. The sections were exposured for 5 weeks in the dark room, and were deveoped in D-19 developer. The number of the labeled cells in the areas of the white pulp, the red pulp and the marginal zone (mean number of labeled cells per 0.21 mm2) were observed and calculated. The results were as follow: 1. On histological study, in the spleen of mitomycin C treated group, vacuoles containing pyknotic nuclei were observed more frequently than in those of 5-fluorouracil treated group. Whereas in the AG60 treated group, morphological changes of the spleen were not observed. 2. On autoradiographic study, most of the labeled cells were located near the marginal zone of the spleen. 3. On autoradiographic study, number of the labeled cells of normal control, experimental control, 5-fluorouracil-treated, mitomycin C-treated, AG60 (5 mg/kg)-, and AG60 (30 mg/kg)-treated groups were 214.0+/-56.87, 235.7+/-59.69, 331.0+/-67.20, 137.0+/-33.48, 124.6+/-34.28, and 64.9+/-16.26, respectively. 4. In the mitomycin C treated group and AG60 (5 mg/kg or 30 mg/kg) treated group, labeled cells containing a few silver grains of (3)H-thymidine were observed more frequently than in those of the normal and experimental control groups. But in the 5-fluorouracil treated group, number of the heavy labeled cells were observed more frequently than in those of the normal and experimental control groups. From the above results, DNA synthesis, in the cells of the marginal zone were more active than in the cells of the white pulp or the red pulp. And mitomycin C and AG60 may suppress the DNA synthesis of the splenic tissues. Especially, AG60 does not results any histological defect on the splenic tissues. These result suggest that AG60 is expected as one of most effective anticancer drugs.

Immunohistochemical Expression of p53 and Cathepsin D in Prostatic Carcinoma / 대한암학회지

PURPOSE: To evaluate the prognostic significances of p53 and cathepsin D in the prostatic carcinoma, we compared them to other prognostic factors, such as nuclear grade and clinical stage. MATERIALS AND METHODS: The material consisted of 40 paraffin-embedded, primary prostate carcinomas. We examined the expression of p53 and cathepsin D using immunohistochemical staining and compared their expression with the grade and stage. RESULTS: The expressions of p53 were noted in the nucleus of tumor cells and cathepsin D were noted in the cytoplasm of tumor cells. Thirteen of 40 tumors were positive for p53. There were more expressing p53 in samples (40%) from prostatic cancer with a high Gleason score group than in samples (28%) from prostatic cancer with low Gleason score group. The expression of p53 was 22% in clinical stage B and C groups and 35% in clinical stage D group. These results showed that p53 expression was not statistically correlated with Gleason score and clinical stage, but there were trends to increased p53 expression with high Gleason score and progressed clinical stage (p>0.05). Progressed clinical stage group showed higher expression of cathepsin D than early clinical stage group. However, there were no statistical correlations between expression of cathepsin D and Gleason score, and clinical stage (p>0.05). CONCLUSION: These results suggest that the overexpression of p53 and cathepsin D may be associated with tumor differentiation and clinical stage, but have limited prognostic value in prostatic carcinoma.

A Clinical Study of Acute Graft-versus-host disease after Allogeneic Bone Marrow Transplantation: Cutaneous Manifestation / 대한피부과학회지

BACKGROUND: Acute graft-versus-host disease(GVHD) has been a major obstacle of successful allogeneic bone marrow transplantation(BMT). Because the onset of cutaneous graft-versus-host reaction (GVHR) is usually the earliest and certainly the most common form of the disease, cutaneous manifestation of acute GVHD is important to detect as early as possible. Except for a few case reports, there has been no clinical study of acute cutaneous GVHR in Korea so far. OBJECTIVE: We investigated the clinical characteristics of acute cutaneous GVHR in recepients receiving allogeneic BMT from human leukocyte antigen(HLA)-identical sibling donor. METHODS: On the basis of the patients' charts and the records of Catholic BMT center, we investigated the incidence of acute GVHD and the clinical manifestation of acute GVHD occurring in 102 patients of whom 66 patients were confirmed by histopathology. RESULTS: 1. The incidence of acute GVHD was 23.5 %, and the incidence of male and female were 22.4 %, 25.0 % respectively. 2. The cutaneous GVHR usually developed within 10-40 days after BMT and usually the earliest and most common form of the GVHD. 3. The most common cutaneous involvement site were upper extremities and chest and 51.4 % of cutaneous GVHR patients complained pruritus, pain and tenderness. 4. The over all clinical grade was lower than that in western countries. CONCLUSION: We speculate that the lower incidence of acute GVHD and milder clinical characteristics than the reports in the western countries might be related to differences in genetic background.

Morphological Study on the Spleen following the Administration of 5-Fluorouracil or Mitomycin C / 체질인류학회지

This experiment was performed to evaluate the morphological responses of 5-fluorouracil or mitomycin C on the spleen of the mice. 5-fluorouracil (60 mg/kg) or mitomycin C (400 microgram/kg) were injected subcutaneously every other day, and the animals were sacrificed at 1 week and 2 weeks following the first injection. Pieces of the tissue were taken from the spleen, fixed in 10% neutral formalin for light microscopy. The paraffin sections stained with hematoxylin-eosin, Masson-trichrome, Bielschowsky's silver impregnation or aldehydefuchsin. For electron microscopy, the tissues were prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde followed by post-fixed with 1% osmium tetroxide. The ultrathin sections stained with uranyl acetate and lead citrate. The observed results were as follows: 1. On histological study, in the mitomycin C treated group, macrophages which contain pyknotic nuclei were observed more frequently than in those of 5-fluorouracil treated group. 2. In the 5-fluorouracil treated group, positive reactions to Masson-trichrome and Bielschowsky's silver impregnation were observed in the splenic capsule and traculae at the 1 week, and weak postive stains were observed at the 2 weeks. 3. In the mitomycin C and the 5-fluorouracil group, positive staining reaction to aldehyde-fuchsin were observed in splenic capsule, trabeculae and around artery at the 1 week and 2 weeks. 4. On the ultrastructural study, distended cisternae of granular endoplasmic reticula were observed frequently at 1 week. 5. In the mitomycin C treated group, myelin figures in the lymphocytes and reticular cells were observed more frequently than in those of 5-fluorouracil treated group. From the above results, it was concluded that lymphocytes and reticular cells of the spleen were slightly damaged by 5-fluorouracil or mitomycin C, and mitomycin C seems more harmful on the spleen than 5-fluorouracil does.

A Case of Hand-Foot-Mouth Disease in An Adult after Bone Marrow Transplantation / 대한피부과학회지

Hand-Foot-Mouth disease is a highly contagious disease most often seen in children. It is caused most commonly by the coxsackie virus A16. Other enterovirus have been implicated. A 41-year-old woman was presented with painful multiple vesicles on oral mucosa, tongue, both hands and feet. She had recieved a bone marrow transplant 16 months ago and had recieved immunosuppressive drugs. Immunosuppression may have a role in the development of Hand-Foot-Mouth disease. Prompt and accurate diagnosis is important because serious sequelaes are common in an elderly individual and immunocompromised patient.

Four Cases of Acquired Ichthyosis after Bone Marrow Transplant / 대한피부과학회지

Acquired ichthyosis is similar to ichthyosis vulgaris clinically and histologically, but it may develop in patients with several systemic disease and secondary to multiple drugs. Acquired ichthyosis was observed in four patients who had recieved bone marrow transplantation. Immunosuppression or abnormality of the immune system after bone marrow transplants may have a role in the pathogenesis of acquired ichthyosis.