Ultrastructure changes of the pituitary gland of rat after garamicin treatment

An antibiotic drug of the aminoglycoside group gentamicin [commercial name garamycin] was used in the present study. The ultrastructural changes induced in pituitary gland of female albino rat by intraperitoneal injection of daily 5 doses double therapeutic dose [1O mg/kg body weight] of garamycin were studied and examined on the 5th day post treatment. Garamycin, affected the releasing and synthesising activity of prolactin, leutinizing hormone [LH], follicular stimulating hormone [FSH], growth hormone [GH] and adrenocorticotrophic hormone [ACTH]. Prolactin and leutinizing hormones stimulate estrogen and progesterone, follicular stimulating hormone, stimulus ovary follicles production, while growth hormone for development and ACTH stimulates the functions of the adrenal cortex. Post-treatment pathological changes appeared in most secretory cells of the pituitary glands. Generally, cells appeared degranulated with dilatation of RER and Golgi apparatus, swollen and aggregation of mitochondria and irregularity of cell membrane. Nuclear changes were also prominent

Cytological study on the effect of the food preservative sodium nitrite and pyrethoid insecticide polytrin on some mammalian organs

The purpose of the present work was to study the synergestic effect of food preservative sodium nitrite and pyrethroid group of insecticide "polytrin" on the Golgi apparatus of the liver, kidney and spleen cells of male albino rat, and to study the effect of each of those chemicals separately. Polytrin was injected intraperitoneally with a single sublethal dose [1/50 LD50] for two days and sodium nitrite was given at a dose of [2 g/L of water] daily for 90 days by the oral route. The Golgi apparatus has proved to be highly sensitive to polytrin and sodium nitrite treatment. Fragmentation, atrophy and coarse of Golgi apparatus appeared to be a consequence of the destruction of the membrane systems or the suppression of the lipoprotein synthesis necessary for membrane formations

Curative role of isologous bone marrow transplantation on radiation induced histochemical changes in bone marrow of rat

This work is a comparative study of radiation injury on bone marrow enzymes; namely, alkaline phosphatase and peroxidase, and possible control by the isologous bone marrow transplantation. The present study showed increased levels of alkaline phosphatase and peroxidase enzymes in bone marrow cells after radiation exposure. The level of enzymatic activity under investigation could be significantly restored over 3 weeks post-exposure of 6 Gy of gamma irradiation. The results obtained demonstrated the efficacy of bone marrow transplantation for replacement or proliferation of injured hematopoietic tissue component and, consequently, more rapid restoration of the normal levels of enzymatic activities in the irradiated bone marrow