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Objective To observe the antidiarrheal and analgesic effects of Dingguier paste on the mouse. Methods Tho diarrhea mouse models were made by senna leaf or castor oil to detect the antidiarrheal effect of Dingguier paste combination with other drugs. The hot plate test and acetic acid test were used to observe the analgesic effect of Dingguier oral medicine and Dingguier paste in combined use with other drugs. Results Compared with senna leaf model group, Dingguier paste low-dose, middle-dose, high-dose combined with diphenoxylate, Bupi-Yichang pill and Dingguier oral medicine group could decrease the number of feces in 2 h [Number of each group feces was(0.50±1.27), (2.27±2.90), (1.18±1.83);(2.00±2.26), (3.40±2.17), (3.82±1.72);(0.73±1.56), (1.91±2.95), (2.55±2.11)]. Dingguier paste low-dose and high-dose combined with diphenoxylate could decrease the number of feces in 4h [Number of each group feces was (6.70±2.11), (6.27±3.20)]. Dingguier oral group decrease the number of feces in 6h [Number of feces was (6.91±2.77)]. Compared with castor oil model group, Dingguier paste low-dose combined with diphenoxylate could obviously decrease the number of feces in 2 h, 4 h and 6 h [Number of each group feces was(0.45±0.82), (0.45±0.82), (4.27±2.15)]. Dingguier paste middle-dose combined with diphenoxylate could decrease the number of feces in 6 h [Number of feces was (4.64±2.45)]. Dingguier oral group decrease the number of feces in 2 h, 4 h, 6 h [Number of each group feces was (0.45±0.82), (2.91±2.07), (4.27±2.15)].Compared with normal group, the threshold of pain stimulated by hot plate of the Dingguier paste each dose combined with rotundin could increased remarkable after 30 min, 60 min, 90 min and 120 min[The time of Dingguier paste low-dose was(52.32 ± 17.29)s, (56.24 ± 12.48)s, (57.28 ± 6.61)s, (58.58 ± 4.70)s; the time of Dingguier paste middle-dose was (48.27±19.35)s, (54.92±12.59)s, (55.91±11.48)s, (55.15±12.49)s;the time of Dingguier paste high-dose was (49.15±15.69)s, (56.24±11.89)s, 60 s is(59.08±2.93)s]. Compared with normal group, the eclipse time of pain irritated by acetic acid of the Dingguier paste middle-dose combined with indomethacin could increased remarkable(14.72±5.99)min, the writhing frequency could decrease of the Dingguier paste each dose combined with indomethacin[Number of each dose writhing frequency was (7.62±8.31), (3.62± 6.14), (11.25±9.46)]. Conclusion Dingguier paste in the combination use with other drugs and Dingguier oral medicine may have some antidiarrheal and analgesia effects.
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BACKGROUND: Constipation, especially that caused by spleen insufficiency in transportation, often not responses to common purgatives, however,the therapy to strengthen the spleen and regulate qi so as to promote transportation and dredge stasis may be an ideal way to resolve the problem.OBJECTIVE: To investigate the effect of zhizhu decoction, a classic famous prescription, on gastric motility of mice with spleen insufficiency constipationDESIGN: A randomized controlled trial.SETTING: Department of Traditional Chinese Medicine (TCM) of Guangdong Medical College and Spleen-stomach Institute of Guangzhou University of Traditional Chinese Medicine.MATERIALS: The experiment was completed from June to December2002 at Spleen-stomach Institute of Guangzhou University of Traditional Chinese Medicine. Totally 328 NIH mice of general grade were chosen.METHODS: Mouse models with spleen insufficiency constipation were set up by irregular diet and overstrain combined with water deprivation. The influence of zhizhu decoction in large, middle and small doses on defecation in both spleen insufficiency mice and normal mice was investigated, taking Chinese patent drug bianmi tong (bowel-relaxing) as a positive control. In observation of normal mice defecation, there were five groups of large dose,middle dose and small dose of zhizhu decoction, bianmi tong and normal control. In observation of defecation of spleen insufficiency constipation mice, there were six groups: the above five and a natural recovering. In observation of loading mice trial, besides the above five groups, there was an atropine group. After administration, the time when the mice first defecated dark stool, the number of stool grains and the stool quantity were observe;and the percentage of small intestine advance was calculated.MAIN OUTCOME MEASURES: [1] Observation of the effect of zhizhu decoction on the defecation in both normal and spleen insufficiency mice.[2] The influence of zhizhu decoction on bowel advance in both normal and atropine loading miceRESULTS: Totally 328 mice were involved in the trial and 319 entered into the final result analysis, the other 9 died because of mistake in administration by gavage. The effect of zhizhu decoction on the defecation: [1] For normal mice, compared with the distilled group, in the zhizhu decoction groups of various doses the time when the mice first defecated dark stool was obviously reduced, and the number of stool grains and stool quantity were obviously increased, but the effect in the large and middle dose groups was best. [2] Compared with the normal group, in the model group the time when the mice first defecated dark stool was obviously increased, and the number of stool grains and stool quantity were obviously decreased. Zhizhu decoction in large and middle doses could obviously reduce the time when the mice first defecated dark stool, and increase the number of stool grains and stool quantity, the effect was better than that of the control drug bianmi tong. The influence ofzhizhu decoction on intestinal advance in mice: [1]Zhizhu decoction in various doses could obviously increase the rate of intestinal advance compared with distilled water group for normal mice. [2]Adding atropine load could reduce the rate of intestinal advance compared with the normal control group, and the percentage in all zhizhu decoction groups was obviously increased compared with the atropine group.CONCLUSION: Zhizhu decoction can reduce the time when the mice first defecated dark stool, increase the number of stool grains and stool quantity were obviously increased to both normal mice and mice with spleen insufficiency mice; it can also increase the intestinal advance rate in normal mice, and had a good antagonism against the inhibition of atropine to intestinal advance. In the trials the effect of zhizhu decoction in large and middle doses was more obvious, with a certain dose-effect relationship, the effect was better than that of the control drug bianmi tong.
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【Objective】To observe the effect of Kuijieling Decoction(KD) on NF-?B p65 protein expression in colonic mucosa and serum tumor necrosis factor ?(TNF-?) content of ulcerative colitis rats.【Methods】Sixty SD rats were randomized into 6 groups: normal group,model group,salicylazosulfapyridine(SASP,0.5g?kg-1?d-1) group and three KD(18.3,9.2 and 4.6 g?kg-1?d-1 respectively) groups.Except the normal group,the rats received enteroclysis of trinitro-benzene-sulfonic acid(TNBS) to induce ulcerative colitis models.Serum content of TNF-? was detected by enzyme-linked immunosorbent assay(ELISA).Then the rats were executed to make tissue section.The tissue sections were stained by immunohistochemistry and the cells with positive expression of NF-?B p65 protein were counted under microscope.【Results】The serum level of TNF-? and the cell number of positive expression of NF-?B p65 protein in high-and moderate-dose KD groups were lower than those in the model group(P
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Achievements of the 30-year research work on Pi-Wei Doctrine were summarized. They are: (1) ratio of salivin activity and excretion rate of D-xylose in patients with spleen deficiency are different from those in the normal subjects;(2) the principle of syndrome differentiation and treatment is embodied in the methodology of herbal medicine pharmacology for complex prescription; (3) the above findings are further confirmed by the research in molecular and cellular level which indicates that TCM syndromes and pharmacological mechanisms of herbal medicines have their material basis and the regulatory effect of complex prescription on TCM syndromes is actually the pharmacological mechanism of herbal medicines. It is suggested that the research of herbal medicine should be based on the guidance of traditional Chinese medicinal theory and carried out in clinical practice.
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To observe the effect of Weiyanxiao Granule (WG) in counteracting experimental gastric ulcer,acute lesion of gastric mucosa and to investigate its anti-inflammation and analgesic actions.Acute gastric ulcer models induced by stress or indomethacin, chronic gastric ulcer models induced by acetic acid and models with acute lesion of gastric mucosa induced by hydrochloride-ethanol or 0.2 mol?L -1 sodium hydroxide were used to observe the effect of WG in counteracting experimental gastric ulcer and acute lesion of gastric mucosa. Anti-inflammation action of WG was observed on mouse models with auricular swelling induced by dimethylbenzene, rat models with pedal swelling induced by white egg and rat models with granuloma induced by embedding tampon. Hot plate method and body-torsion test were used to observe the analgesic action of WG. As compared with the control group, WG 12.3?g?kg -1 and 24.6?g?kg -1 could decrease the ulcer index in rats with acute gastric ulcer (P
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[Objective] To observe the regulatory effect of astragalosides (AST) on the binding capacity of gastrin receptor in gastric parietal cells (GPC) of rats with spleen deficiency. [Methods] SD rats were given gastric gavage of Radix et Rhizoma Rhei (Dahuang) decoction 10 g/kg to induce spleen deficiency. Gastric parietal cells were isolated and purified by Lewin gastric sac method and Percoll density gradient separation method. 2 mL suspension of GPC from rat models was incubated in vitro with AST 20 ?L at the concentrations of 10, 20 and 30 g/L respectively. Suspension of GPC from rat models incubated with phosphate buffered saline (PBS) served as the model group and suspension of GPC from the normal rats incubated with PBS as the normal group. The binding capacity of gastrin receptor in GPC was detected by radioligand receptor assay. [Results] The binding sites were 262.29?60.80 in the model group, much lower than 443.93?133.58 in the normal group (P
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Objective To observe the effect of Sijunzi Decoction(SD) and Huangqi Sijunzi Decoction(HSD) on mitochondrial succinate dehydrogenase,cytochrome oxidase activity of liver in rats with spleen asthenia,thus to reveal the pathogenesis mechanism of spleen asthenia from energy metabolism,and to clarify the therapeutic mechanism of spleen-invigorating and Qi-tonifying herbs.Methods Forty SD rats were randomly divided into 4 groups:normal control group,spleen-asthenia model group,SD(10.5 g/kg) group and HSD(18 g/kg) group,10 rats in each group.Except that the normal control group,the rats in the other groups were fed with half-full diet once every other day and with gastic gavage of Xiaochengqi Decoction 60 g/kg every day to establish animal model of spleen asthenia.After 15 days,the model rats were administered the corresponding drugs according to the experimental design for 4 continuous weeks.The changes of mitochondrial succinate dehydrogenase,cytochrome oxidase activity of liver were measured,and the general status of the rats were observed.Results Compared with the normal control group,the level of mitochondrial succinate dehydrogenase and cytochrome oxidase activity in liver of spleen asthenia group were decreased obviously(P
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Objective To establish a quality standard for Kangtai Capsule. Methods Kangtai Capsule was identified by TLC; the effective components in Kangtai Capsule were determined by HPLC. Results The relevant spots in Radix Paeoniae Alba, Radix Saposhnikoviae and Radix Aucklandlae were identified by TLC. the content of Paeoniflorin showed a good linearity in the range of 0.204～ 2.040 ? g, the average recovery rate was 97.9 % and RSD was 0.88 % . Conclusion This method is simple, feasible and repeatable, and can be used for quality control of Kangtai Capsule.
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[Objective] To observe the effects of blood-activating and stasis-removing (BASR) herbs on the proliferation and apoptosis of gastric mucosa cells (GMC) in experimental chronic atrophic gastritis (CAG) rats. [Methods] CAG rat models were established with comprehensive method and then were given BASR herbs. Argyrophilic protein of nucleolar organizer regions ( AgNOR) count was carried out after silver nitrate staining. The expression of proliferating cell nuclear antigen (PCNA) was examined with immunohistochemical method and apoptosis was detected by in-situ cell-end labeling method. [Results] The number of AgNOR and the indexes of PCNA and apoptosis were increased in CAG model rats ( P
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[Objective] To establish a determination method for isolated gastrointestinal intra-cavity perfusion pressure and to explore its application in pharmacological experiment. [Methods] Determination method for isolated gastrointestinal intra-cavity perfusion pressure was established according to motility of the isolated rat gastric muscle strip and water-sac perfusion manometry and ileum peristalsis of Guinea pigs. Intra-cavity pressure of isolated gastrointestinal canal of rat and guinea pig was observed. [Results] Gastric intra-cavity pressure was markedly increased as added with various doses of acetylcholine (1.25, 2.5, 3.25, 5, 6.25 and 7.5 ?g/mL respectively) in nutrient solution. The ileum intra-cavity pressure was also increased as added with various doses of histamine (0.125, 0.25, and 0.5 ?g/mL respectively) . The effects were in a dose-effect manner and the differences were significant as compared with distilled water group ( P
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Objective To explore the changes of proteins level of TLR2 in colonic mucosa of ulcerative colitis( UC) model rats and to observe the effects of Kuijieling Decoction( KD) on TLR2. Methods UC model rats were induced by TNBS. The rats were randomly divided into six groups as follows: normal control (NC) group, model control (MC) group, lowdose Kuijieling (KLD) group, mediumdose Kuijieling( KMD) group, high- dose Kuijieling (KHD) group and SASP group. After 10- days treatment the rats were killed to get their colonic mucosa and extract the whole- cell proteins. Western blot technique was used to detect the TLR2 protein level. ? - actin was used as the internal index. Relative expression level of target protein was calculated from the gray scale ratio of TLR2 and ? - actin. Results Relative protein expression level of TLR2 in MC group was significantly higher than that in NC group (P
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Objective To observe the therapeutic effect of Kuijieling Decoction on experimental ulcerative colitis rats.Methods Ulcerative colitis rat model was induced by enteroclysis with trinitro-benzene-sulfonic acid(TNBS).Three dosages(18.3 g/kg,9.2 g/kg,4.6 g/kg)of Kuijieling Decoction were used to treat the model rats.After treatment,the serum of rats were obtained to detect the level of IL-1? by enzyme-linked immunosorbent assay.Then the rats were killed for the observation of the colonic pathologic changes by naked eye and for the estimation of the damage scores of colon mucosa.Finally,the colon tissue was fixed to make pathological section and the damage index of colon mucosa was observed under microscope.Results The tissue damage scores of high-and moderate-dosage groups of Kuijieling Decoction were(2.41? 0.79)and(2.83? 0.71)respectively,and were significantly lower than that of model control group(3.75? 0.75;P
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Objective Effects of Weiyanxiao on basal gastric- acid secretion in anesthetized rats were investigated.Methods pH value of gastric perfusate in rat models of gastric perfusion were detected with a precise acidity meter to evaluated the effects of Weiyanxiao on basal gastric- acid secretion and gastrin- induced acid secretion.Results Small- dose Weiyanxiao (12.3 g/kg) had no significant influence on basal gastric- acid secretion and has slight inhibition on high- level gastric- acid secretion induced by gastrin ;large- dose Weiyanxiao (24.6g/kg) could restrain basal gastric- acid secretion (P
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AIM To establish radioligand binding assay of gastrin receptor in rat gastric parietal cell. METHODS Using 125 I labeled [Leu 15 ] gastrin 17 I as the radioligand, rat gastric parietal cells as the receptor preparations. RESULTS In the range of the study, a Scatchard plot of the binding was made by Ligand program with an equilibrium K d of approximately 1 249?10 -10 mol?L -1 and a maximum binding capacity of 4 4604?10 -12 mol?L -1 . The amount of gastrin binding was strongly associated with the cell concentration of rat gastric parietal cell preparations ranged from 0 25?10 9 to 2?10 9 cells?L -1 . The variant coefficent of the binding assay was 7 04% within the same sample. The competitive inhibiting analyses showed that 125 I gastrin was bound to gastric parietal cell preparations with a high specificity. CONCLUSION The radioligand binding assay of gastrin receptor in rat gastric parietal cell preparations meets the criteria for establishing receptor binding assay.